                         SEQUENCE LISTING

<110>  Danmarks Tekniske Universitet
 
<120>  Mutant RNAase E for enhancing recombinant protein expression

<130>  P3003PC00

<150>  EP 21177466.6
<151>  2021-06-02

<160>  92    

<170>  PatentIn version 3.5

<210>  1
<211>  3186
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(3186)
<223>  E. coli rne (wild type) encoding RNase E

<400>  1
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc gac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agc       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtc tgc ccg cgc tgc tcc ggt acc ggt acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tat ctg ctg aat gaa aaa cgt tct gcg gtg aat gcc att gaa acg       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtt cgc tgc gtg att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg cgt aaa ggg gaa gaa acg ccg       1488
Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro           
                485                 490                 495               

acc tta agc tac atg ctg ccg aag ctg cat gaa gaa gcg atg gcg ctg       1536
Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu           
            500                 505                 510                   

ccg tct gaa gaa gag ttc gct gaa cgt aag cgt ccg gaa caa cct gcg       1584
Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala           
        515                 520                 525                       

ctg gca acc ttt gcc atg ccg gat gtg ccg cca gcg cca acc cca gct       1632
Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala           
    530                 535                 540                           

gaa cct gcc gcg cct gtc gta gcc cca gca cct aaa tct gca ccg gca       1680
Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala           
545                 550                 555                 560           

aca cca gcc gct cct gcc caa cct ggg ctg ttg agc cgc ttc ttc ggc       1728
Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly           
                565                 570                 575               

gca ctg aaa gcg ctg ttc agc ggt ggt gaa gaa acc aaa ccg tcc gag       1776
Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu           
            580                 585                 590                   

caa cca aca ccg aaa gca gaa gcg aaa ccg gaa cgt caa cag gat cgt       1824
Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg           
        595                 600                 605                       

cgc aag cct cgt cag aac aac cgc cgt gac cgt aat gag cgc cgc gac       1872
Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp           
    610                 615                 620                           

acc cgt agt gaa cgt act gaa ggc agc gat aat cgc gaa gaa aac cgt       1920
Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg           
625                 630                 635                 640           

cgt aat cgt cgc cag gca cag cag cag act gcc gag acg cgt gag agc       1968
Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser           
                645                 650                 655               

cgt cag cag gtt gag gta acg gaa aaa gcg cgt acc acc gac gag cag       2016
Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln           
            660                 665                 670                   

caa gcg ccg cgt cgt gaa cgt agc cgc cgc cgt aat gat gat aaa cgt       2064
Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg           
        675                 680                 685                       

cag gcg caa caa gaa gcg aag gcg ctg aat gtt gaa gag caa tct gtt       2112
Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val           
    690                 695                 700                           

cag gaa acc gaa cag gaa gaa cgt gta cgt ccg gtt cag ccg cgt cgt       2160
Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg           
705                 710                 715                 720           

aaa cag cgt cag ctc aat cag aaa gtg cgt tac gag caa agc gta gcc       2208
Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala           
                725                 730                 735               

gaa gaa gcg gta gtc gca ccg gtg gtt gaa gaa act gtc gct gcc gaa       2256
Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu           
            740                 745                 750                   

cca att gtt cag gaa gcg cca gct cca cgc aca gaa ctg gtg aaa gtc       2304
Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val           
        755                 760                 765                       

ccg ctg cca gtc gta gcg caa act gca cca gaa cag caa gaa gag aac       2352
Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn           
    770                 775                 780                           

aat gct gat aac cgt gac aac ggt ggc atg ccg cgt cgt tct cgc cgc       2400
Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg           
785                 790                 795                 800           

tcg cct cgt cac ctg cgc gta agt ggt cag cgt cgt cgt cgc tat cgt       2448
Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg           
                805                 810                 815               

gac gag cgt tat cca acc cag tcg cca atg ccg ttg acc gta gcg tgc       2496
Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys           
            820                 825                 830                   

gcg tct ccg gaa ctg gcc tct ggc aaa gtc tgg atc cgc tat cca att       2544
Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile           
        835                 840                 845                       

gta cgt ccg caa gat gta cag gtt gaa gag cag cgc gaa cag gaa gaa       2592
Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu           
    850                 855                 860                           

gta cat gtg cag ccg atg gtg act gag gtc cct gtc gcc gcc gct atc       2640
Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile           
865                 870                 875                 880           

gaa ccg gtt gtt agc gcg cca gtt gtt gaa gaa gtg gcc ggt gtc gta       2688
Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val           
                885                 890                 895               

gaa gcc ccc gtt cag gtt gcc gaa ccg caa ccg gaa gtg gtt gaa acg       2736
Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr           
            900                 905                 910                   

acg cat cct gaa gtg att gct gcc gcg gta act gaa cag ccg cag gtg       2784
Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val           
        915                 920                 925                       

att acc gag tct gat gtt gcc gta gcc cag gaa gtt gca gaa caa gca       2832
Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala           
    930                 935                 940                           

gaa ccg gtg gtt gaa ccg cag gaa gag acg gca gat att gaa gaa gtt       2880
Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val           
945                 950                 955                 960           

gtc gaa act gct gag gtt gta gtt gct gaa cct gaa gtt gtt gct caa       2928
Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln           
                965                 970                 975               

cct gcc gcg cca gta gtc gct gaa gtc gca gca gaa gtt gaa acg gta       2976
Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val           
            980                 985                 990                   

gct gcg gtc gaa cct gag gtc acc  gtt gag cat aac cac  gct acc gcg     3024
Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala         
        995                 1000                 1005                     

cca atg  acg cgc gct cca gca  ccg gaa tat gtt ccg  gag gca ccg        3069
Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro            
    1010                 1015                 1020                        

cgt cac  agt gac tgg cag cgc  cct act ttt gcc ttc  gaa ggt aaa        3114
Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys            
    1025                 1030                 1035                        

ggt gcc  gca ggt ggt cat acg  gca aca cat cat gcc  tct gct gct        3159
Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala            
    1040                 1045                 1050                        

cct gcg  cgt ccg caa cct gtt  gag taa                                 3186
Pro Ala  Arg Pro Gln Pro Val  Glu                                         
    1055                 1060                                             


<210>  2
<211>  1061
<212>  PRT
<213>  E. coli

<400>  2

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu 
            580                 585                 590         


Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg 
        595                 600                 605             


Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp 
    610                 615                 620                 


Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg 
625                 630                 635                 640 


Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser 
                645                 650                 655     


Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln 
            660                 665                 670         


Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg 
        675                 680                 685             


Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val 
    690                 695                 700                 


Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg 
705                 710                 715                 720 


Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala 
                725                 730                 735     


Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu 
            740                 745                 750         


Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val 
        755                 760                 765             


Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn 
    770                 775                 780                 


Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg 
785                 790                 795                 800 


Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg 
                805                 810                 815     


Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys 
            820                 825                 830         


Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile 
        835                 840                 845             


Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu 
    850                 855                 860                 


Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile 
865                 870                 875                 880 


Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val 
                885                 890                 895     


Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr 
            900                 905                 910         


Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val 
        915                 920                 925             


Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala 
    930                 935                 940                 


Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val 
945                 950                 955                 960 


Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln 
                965                 970                 975     


Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val 
            980                 985                 990         


Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala 
        995                 1000                 1005             


Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro 
    1010                 1015                 1020             


Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys 
    1025                 1030                 1035             


Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala 
    1040                 1045                 1050             


Pro Ala  Arg Pro Gln Pro Val  Glu 
    1055                 1060     


<210>  3
<211>  3240
<212>  DNA
<213>  Pseudomonas putida


<220>
<221>  CDS
<222>  (1)..(3240)
<223>  P. putida rne (wild type) encoding RNAse E

<400>  3
atg aaa aga atg ctg att aac gca act caa ccc gaa gag ttg cgt gta         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Pro Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctg gtg gac ggc caa cgc ctc tac gac ctg gac atc gag tcc ggc         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Gly           
            20                  25                  30                    

gca cgt gag cag aaa aag gcc aac atc tac aaa ggc aag atc acc cgc        144
Ala Arg Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

atc gaa ccc agc ctc gaa gcc gcc ttc gtc gac ttc ggt tcc gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Phe Gly Ser Glu Arg           
    50                  55                  60                            

cac ggc ttc ctg ccg ctg aaa gaa atc tcc cgc gaa tac ttc aag aaa        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ser Arg Glu Tyr Phe Lys Lys           
65                  70                  75                  80            

gcc ccc gaa ggc cgg gtg aac atc aag gaa gtg ctc agc gaa ggc cag        288
Ala Pro Glu Gly Arg Val Asn Ile Lys Glu Val Leu Ser Glu Gly Gln           
                85                  90                  95                

gaa gtc atc gtc cag gtc gag aag gaa gag cgc ggc aac aaa ggc gcc        336
Glu Val Ile Val Gln Val Glu Lys Glu Glu Arg Gly Asn Lys Gly Ala           
            100                 105                 110                   

gcc ctc acc acc ttc atc agc ctg gct ggc cgc tac ctg gtg ctg atg        384
Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Arg Tyr Leu Val Leu Met           
        115                 120                 125                       

ccc aac aac ccg cgt gct ggc ggc atc tcc cgc cgc atc gaa ggc gaa        432
Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu Gly Glu           
    130                 135                 140                           

gag cgc aac gaa ctg cgc gaa gcc ctg aac ggc ctg acc gtg ccg ggc        480
Glu Arg Asn Glu Leu Arg Glu Ala Leu Asn Gly Leu Thr Val Pro Gly           
145                 150                 155                 160           

gac atg ggc ctg atc gtg cgc act gcc ggc ctt ggc cgc agc agc gaa        528
Asp Met Gly Leu Ile Val Arg Thr Ala Gly Leu Gly Arg Ser Ser Glu           
                165                 170                 175               

gaa atg cag tgg gac ctc gac tac ctg ctg cag ctg tgg acc gcc atc        576
Glu Met Gln Trp Asp Leu Asp Tyr Leu Leu Gln Leu Trp Thr Ala Ile           
            180                 185                 190                   

aag gaa gca tcc ctg gac cgc gcc gcg cca ttc ctg atc tac cag gaa        624
Lys Glu Ala Ser Leu Asp Arg Ala Ala Pro Phe Leu Ile Tyr Gln Glu           
        195                 200                 205                       

agc aac gtc atc atc cgc gcc atc cgc gac tac ctg cgc cag gac atc        672
Ser Asn Val Ile Ile Arg Ala Ile Arg Asp Tyr Leu Arg Gln Asp Ile           
    210                 215                 220                           

ggt gaa gtg ctg atc gac agc atc gac gcc cag gaa gag gcc ctg acc        720
Gly Glu Val Leu Ile Asp Ser Ile Asp Ala Gln Glu Glu Ala Leu Thr           
225                 230                 235                 240           

ttc atc cgc cag gtg atg ccg cag tac gcc agc aag gtg aaa ctg tac        768
Phe Ile Arg Gln Val Met Pro Gln Tyr Ala Ser Lys Val Lys Leu Tyr           
                245                 250                 255               

gaa gac agc gta ccg ctg ttc aac cgc ttc cag atc gaa agc cag atc        816
Glu Asp Ser Val Pro Leu Phe Asn Arg Phe Gln Ile Glu Ser Gln Ile           
            260                 265                 270                   

gaa acc gcc ttc cag cgc gtg gtc gac ctg ccg tcc ggt ggt tcg atc        864
Glu Thr Ala Phe Gln Arg Val Val Asp Leu Pro Ser Gly Gly Ser Ile           
        275                 280                 285                       

gtg atc gac ccg acc gaa gcc ctg gtg tct atc gac atc aac tcg gcg        912
Val Ile Asp Pro Thr Glu Ala Leu Val Ser Ile Asp Ile Asn Ser Ala           
    290                 295                 300                           

cgc gcc acc aaa ggc agc gat atc gaa gaa acc gcc ctg cag acc aac        960
Arg Ala Thr Lys Gly Ser Asp Ile Glu Glu Thr Ala Leu Gln Thr Asn           
305                 310                 315                 320           

ctg gaa gcg gcg gag gaa atc gcc cgc cag ctg cgc ctg cgt gac atc       1008
Leu Glu Ala Ala Glu Glu Ile Ala Arg Gln Leu Arg Leu Arg Asp Ile           
                325                 330                 335               

ggc ggc ctg atc gtg atc gac ttc atc gac atg acc ccg gcg aaa aac       1056
Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro Ala Lys Asn           
            340                 345                 350                   

cag cgc gcc gtt gaa gaa cgc gtg cgc gaa tgc ctg gaa gcg gac cgt       1104
Gln Arg Ala Val Glu Glu Arg Val Arg Glu Cys Leu Glu Ala Asp Arg           
        355                 360                 365                       

gcc cgc gtg cag gtt ggc cgc atc tcg cgc ttc ggc ctg ctg gaa atg       1152
Ala Arg Val Gln Val Gly Arg Ile Ser Arg Phe Gly Leu Leu Glu Met           
    370                 375                 380                           

tcc cgt cag cgc ctg cgc cca tcg ctg ggc gaa agc agc ggc atc gtc       1200
Ser Arg Gln Arg Leu Arg Pro Ser Leu Gly Glu Ser Ser Gly Ile Val           
385                 390                 395                 400           

tgc cca cgc tgc tcc ggc acc ggc atc atc cgt gac gtg gag tcg ctg       1248
Cys Pro Arg Cys Ser Gly Thr Gly Ile Ile Arg Asp Val Glu Ser Leu           
                405                 410                 415               

tcg ctg gcc atc ctg cgc ctg atc gaa gaa gaa gcc ctg aag gac cgc       1296
Ser Leu Ala Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu Lys Asp Arg           
            420                 425                 430                   

act gcc gaa gtc cgt gca caa gtg cca atc ccg gtg gcc gcg ttc ctg       1344
Thr Ala Glu Val Arg Ala Gln Val Pro Ile Pro Val Ala Ala Phe Leu           
        435                 440                 445                       

ctc aac gag aag cgc aac tcg atc acc aag atc gaa ctg cgc acc cgt       1392
Leu Asn Glu Lys Arg Asn Ser Ile Thr Lys Ile Glu Leu Arg Thr Arg           
    450                 455                 460                           

gcg cgc atc atc atc ctg ccg aac gat cac ctg gaa act ccg cac ttc       1440
Ala Arg Ile Ile Ile Leu Pro Asn Asp His Leu Glu Thr Pro His Phe           
465                 470                 475                 480           

gaa gtc cag cgc ctg cgc gac gac aac ccg gaa gtg ctg aac aac cag       1488
Glu Val Gln Arg Leu Arg Asp Asp Asn Pro Glu Val Leu Asn Asn Gln           
                485                 490                 495               

tcc agc tac gag atc gcc acc acc gaa gcc gaa gag gca ccg cag cag       1536
Ser Ser Tyr Glu Ile Ala Thr Thr Glu Ala Glu Glu Ala Pro Gln Gln           
            500                 505                 510                   

acc gcc acc cgc acc ctg gtt cgc cag gaa gca gcg gtc aag acc gcc       1584
Thr Ala Thr Arg Thr Leu Val Arg Gln Glu Ala Ala Val Lys Thr Ala           
        515                 520                 525                       

ccg gcc cgc acc aac gcg ccg gta ccg gtt gct gaa gag cct cag gct       1632
Pro Ala Arg Thr Asn Ala Pro Val Pro Val Ala Glu Glu Pro Gln Ala           
    530                 535                 540                           

gtt gcc ccg gcc gcc ccg gcc ccg agc gcc ccg gag cca agc ctg ttc       1680
Val Ala Pro Ala Ala Pro Ala Pro Ser Ala Pro Glu Pro Ser Leu Phe           
545                 550                 555                 560           

aaa ggc ctg gtg aag tcg ctg gtc agc ctg ttc gcc ggc aag gac gaa       1728
Lys Gly Leu Val Lys Ser Leu Val Ser Leu Phe Ala Gly Lys Asp Glu           
                565                 570                 575               

cct gca gct gct gca cct gtc gtc gcc gag aaa ccg gcc gcc gag cgc       1776
Pro Ala Ala Ala Ala Pro Val Val Ala Glu Lys Pro Ala Ala Glu Arg           
            580                 585                 590                   

agc ccg cgc aac gag gag cgc cgc aac ggc cgt caa cag agc cgc aac       1824
Ser Pro Arg Asn Glu Glu Arg Arg Asn Gly Arg Gln Gln Ser Arg Asn           
        595                 600                 605                       

cgc aac ggc cgc cgc gac gaa gag cgc aag ccg cgt gaa gag cgt gcc       1872
Arg Asn Gly Arg Arg Asp Glu Glu Arg Lys Pro Arg Glu Glu Arg Ala           
    610                 615                 620                           

gag cgc gcc ccg cgc gaa gag cgc cag cca cgc gag gag cgt gca ccg       1920
Glu Arg Ala Pro Arg Glu Glu Arg Gln Pro Arg Glu Glu Arg Ala Pro           
625                 630                 635                 640           

cgc gaa gag cgt gca cca cgc gaa gaa cgc gcc cca cgc gaa gag cgt       1968
Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg           
                645                 650                 655               

gca cca cgc cag cca cgc gaa gac cgc cgc agc aac cgc ggc gaa gag       2016
Ala Pro Arg Gln Pro Arg Glu Asp Arg Arg Ser Asn Arg Gly Glu Glu           
            660                 665                 670                   

cgc gtg cgc gaa ctg cgt gag ccg ctg gat gcc acc ccg cca gcc gaa       2064
Arg Val Arg Glu Leu Arg Glu Pro Leu Asp Ala Thr Pro Pro Ala Glu           
        675                 680                 685                       

cgc gaa gag cgc cag cca cgt gaa gag cgt gta gcc cgt gaa gaa cgc       2112
Arg Glu Glu Arg Gln Pro Arg Glu Glu Arg Val Ala Arg Glu Glu Arg           
    690                 695                 700                           

gcc cca cgt gaa gag cgt gca cct cgc gaa gaa cgt gct ccg cgt gaa       2160
Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu           
705                 710                 715                 720           

gag cgc gca cct cgc gaa gaa cgt gct ccg cgt gaa gag cgt gct cct       2208
Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro           
                725                 730                 735               

cgc gaa gaa cgt gct ccg cgt gaa gag cgc gca cct cgc gaa gaa cgt       2256
Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg           
            740                 745                 750                   

gcg cca cgc gaa gaa cgc gcc ccg cgc cca cca cgc gaa gag cgc cag       2304
Ala Pro Arg Glu Glu Arg Ala Pro Arg Pro Pro Arg Glu Glu Arg Gln           
        755                 760                 765                       

cca cgc gta gcc gaa gaa gcg gcc gag cag gct gcc gaa ctg gcc gaa       2352
Pro Arg Val Ala Glu Glu Ala Ala Glu Gln Ala Ala Glu Leu Ala Glu           
    770                 775                 780                           

gag caa ctg ccg aac gaa gag ctg ctg caa gac gaa cag gaa ggc acc       2400
Glu Gln Leu Pro Asn Glu Glu Leu Leu Gln Asp Glu Gln Glu Gly Thr           
785                 790                 795                 800           

gat ggc gag cgt ccg cgc cgc cgc tcc cgt ggc cag cgt cgt cgc agc       2448
Asp Gly Glu Arg Pro Arg Arg Arg Ser Arg Gly Gln Arg Arg Arg Ser           
                805                 810                 815               

aac cgt cgt gag cgc cag cgc aac gcc aat ggc gag ctg atc gac ggt       2496
Asn Arg Arg Glu Arg Gln Arg Asn Ala Asn Gly Glu Leu Ile Asp Gly           
            820                 825                 830                   

ggc gaa gag gaa gaa ggc agc gaa gag cag cca caa cag cac cag gcc       2544
Gly Glu Glu Glu Glu Gly Ser Glu Glu Gln Pro Gln Gln His Gln Ala           
        835                 840                 845                       

acc gag ctg ggt gcc gaa ctg gcc gcc ggc ctg gca gtg act gcc gct       2592
Thr Glu Leu Gly Ala Glu Leu Ala Ala Gly Leu Ala Val Thr Ala Ala           
    850                 855                 860                           

gtt gcc agc agc aac atc agc gcc gac gcc gaa gcc cag gcc aac cag       2640
Val Ala Ser Ser Asn Ile Ser Ala Asp Ala Glu Ala Gln Ala Asn Gln           
865                 870                 875                 880           

cag gcc gaa ctg gcc acc gcc gaa atc gct gcc gca gca gag acc gac       2688
Gln Ala Glu Leu Ala Thr Ala Glu Ile Ala Ala Ala Ala Glu Thr Asp           
                885                 890                 895               

aac agc cac gcc gct cag ccg gtc gag aag gct gaa aag gtc gag ccg       2736
Asn Ser His Ala Ala Gln Pro Val Glu Lys Ala Glu Lys Val Glu Pro           
            900                 905                 910                   

gtt gaa gcc gtc gcc aag gct gaa gac gtt gcc gta gcc cca gtg gtg       2784
Val Glu Ala Val Ala Lys Ala Glu Asp Val Ala Val Ala Pro Val Val           
        915                 920                 925                       

gag cag cct gtc agc gag ccg gtt gtc gtg gcc gag gtc act gcc gag       2832
Glu Gln Pro Val Ser Glu Pro Val Val Val Ala Glu Val Thr Ala Glu           
    930                 935                 940                           

ccg gta gtc gaa gtc gct ccg caa cac ctg gtt gaa gaa gcg cct gct       2880
Pro Val Val Glu Val Ala Pro Gln His Leu Val Glu Glu Ala Pro Ala           
945                 950                 955                 960           

gcc gaa ccg gta atc gtc gcc gaa gcc cct gtt gaa acg cct gct gtc       2928
Ala Glu Pro Val Ile Val Ala Glu Ala Pro Val Glu Thr Pro Ala Val           
                965                 970                 975               

gaa gcg ggc gaa atc gaa aaa gct ccg gcc gtg gtt gaa acc gct ccg       2976
Glu Ala Gly Glu Ile Glu Lys Ala Pro Ala Val Val Glu Thr Ala Pro           
            980                 985                 990                   

gtt gcc gag cag cct gca ccg gtt  gtc gaa gcc cag cca  gaa gta gtc     3024
Val Ala Glu Gln Pro Ala Pro Val  Val Glu Ala Gln Pro  Glu Val Val         
        995                 1000                 1005                     

gca gag  cct gca cct gtg gtc  gtc gag cct gcc ccg  gta gag gca        3069
Ala Glu  Pro Ala Pro Val Val  Val Glu Pro Ala Pro  Val Glu Ala            
    1010                 1015                 1020                        

gaa gct  gcc acc gtc atg ctg  gcc aac ggc cgt gcg  ccg aac gac        3114
Glu Ala  Ala Thr Val Met Leu  Ala Asn Gly Arg Ala  Pro Asn Asp            
    1025                 1030                 1035                        

ccg cgt  gaa gtg cgc cgc cgc  aag cgt gag gcc gag  gcc gcc gcc        3159
Pro Arg  Glu Val Arg Arg Arg  Lys Arg Glu Ala Glu  Ala Ala Ala            
    1040                 1045                 1050                        

aaa gcc  gcg cag gaa gct gct  gca gcg acc gag gag  cca gcc ctg        3204
Lys Ala  Ala Gln Glu Ala Ala  Ala Ala Thr Glu Glu  Pro Ala Leu            
    1055                 1060                 1065                        

gaa gcc  gcc gat gag cac aag  cct cat cac agt tga                     3240
Glu Ala  Ala Asp Glu His Lys  Pro His His Ser                             
    1070                 1075                                             


<210>  4
<211>  1079
<212>  PRT
<213>  Pseudomonas putida

<400>  4

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Pro Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Gly 
            20                  25                  30          


Ala Arg Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Phe Gly Ser Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ser Arg Glu Tyr Phe Lys Lys 
65                  70                  75                  80  


Ala Pro Glu Gly Arg Val Asn Ile Lys Glu Val Leu Ser Glu Gly Gln 
                85                  90                  95      


Glu Val Ile Val Gln Val Glu Lys Glu Glu Arg Gly Asn Lys Gly Ala 
            100                 105                 110         


Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Arg Tyr Leu Val Leu Met 
        115                 120                 125             


Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu Gly Glu 
    130                 135                 140                 


Glu Arg Asn Glu Leu Arg Glu Ala Leu Asn Gly Leu Thr Val Pro Gly 
145                 150                 155                 160 


Asp Met Gly Leu Ile Val Arg Thr Ala Gly Leu Gly Arg Ser Ser Glu 
                165                 170                 175     


Glu Met Gln Trp Asp Leu Asp Tyr Leu Leu Gln Leu Trp Thr Ala Ile 
            180                 185                 190         


Lys Glu Ala Ser Leu Asp Arg Ala Ala Pro Phe Leu Ile Tyr Gln Glu 
        195                 200                 205             


Ser Asn Val Ile Ile Arg Ala Ile Arg Asp Tyr Leu Arg Gln Asp Ile 
    210                 215                 220                 


Gly Glu Val Leu Ile Asp Ser Ile Asp Ala Gln Glu Glu Ala Leu Thr 
225                 230                 235                 240 


Phe Ile Arg Gln Val Met Pro Gln Tyr Ala Ser Lys Val Lys Leu Tyr 
                245                 250                 255     


Glu Asp Ser Val Pro Leu Phe Asn Arg Phe Gln Ile Glu Ser Gln Ile 
            260                 265                 270         


Glu Thr Ala Phe Gln Arg Val Val Asp Leu Pro Ser Gly Gly Ser Ile 
        275                 280                 285             


Val Ile Asp Pro Thr Glu Ala Leu Val Ser Ile Asp Ile Asn Ser Ala 
    290                 295                 300                 


Arg Ala Thr Lys Gly Ser Asp Ile Glu Glu Thr Ala Leu Gln Thr Asn 
305                 310                 315                 320 


Leu Glu Ala Ala Glu Glu Ile Ala Arg Gln Leu Arg Leu Arg Asp Ile 
                325                 330                 335     


Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro Ala Lys Asn 
            340                 345                 350         


Gln Arg Ala Val Glu Glu Arg Val Arg Glu Cys Leu Glu Ala Asp Arg 
        355                 360                 365             


Ala Arg Val Gln Val Gly Arg Ile Ser Arg Phe Gly Leu Leu Glu Met 
    370                 375                 380                 


Ser Arg Gln Arg Leu Arg Pro Ser Leu Gly Glu Ser Ser Gly Ile Val 
385                 390                 395                 400 


Cys Pro Arg Cys Ser Gly Thr Gly Ile Ile Arg Asp Val Glu Ser Leu 
                405                 410                 415     


Ser Leu Ala Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu Lys Asp Arg 
            420                 425                 430         


Thr Ala Glu Val Arg Ala Gln Val Pro Ile Pro Val Ala Ala Phe Leu 
        435                 440                 445             


Leu Asn Glu Lys Arg Asn Ser Ile Thr Lys Ile Glu Leu Arg Thr Arg 
    450                 455                 460                 


Ala Arg Ile Ile Ile Leu Pro Asn Asp His Leu Glu Thr Pro His Phe 
465                 470                 475                 480 


Glu Val Gln Arg Leu Arg Asp Asp Asn Pro Glu Val Leu Asn Asn Gln 
                485                 490                 495     


Ser Ser Tyr Glu Ile Ala Thr Thr Glu Ala Glu Glu Ala Pro Gln Gln 
            500                 505                 510         


Thr Ala Thr Arg Thr Leu Val Arg Gln Glu Ala Ala Val Lys Thr Ala 
        515                 520                 525             


Pro Ala Arg Thr Asn Ala Pro Val Pro Val Ala Glu Glu Pro Gln Ala 
    530                 535                 540                 


Val Ala Pro Ala Ala Pro Ala Pro Ser Ala Pro Glu Pro Ser Leu Phe 
545                 550                 555                 560 


Lys Gly Leu Val Lys Ser Leu Val Ser Leu Phe Ala Gly Lys Asp Glu 
                565                 570                 575     


Pro Ala Ala Ala Ala Pro Val Val Ala Glu Lys Pro Ala Ala Glu Arg 
            580                 585                 590         


Ser Pro Arg Asn Glu Glu Arg Arg Asn Gly Arg Gln Gln Ser Arg Asn 
        595                 600                 605             


Arg Asn Gly Arg Arg Asp Glu Glu Arg Lys Pro Arg Glu Glu Arg Ala 
    610                 615                 620                 


Glu Arg Ala Pro Arg Glu Glu Arg Gln Pro Arg Glu Glu Arg Ala Pro 
625                 630                 635                 640 


Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg 
                645                 650                 655     


Ala Pro Arg Gln Pro Arg Glu Asp Arg Arg Ser Asn Arg Gly Glu Glu 
            660                 665                 670         


Arg Val Arg Glu Leu Arg Glu Pro Leu Asp Ala Thr Pro Pro Ala Glu 
        675                 680                 685             


Arg Glu Glu Arg Gln Pro Arg Glu Glu Arg Val Ala Arg Glu Glu Arg 
    690                 695                 700                 


Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu 
705                 710                 715                 720 


Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro 
                725                 730                 735     


Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg Ala Pro Arg Glu Glu Arg 
            740                 745                 750         


Ala Pro Arg Glu Glu Arg Ala Pro Arg Pro Pro Arg Glu Glu Arg Gln 
        755                 760                 765             


Pro Arg Val Ala Glu Glu Ala Ala Glu Gln Ala Ala Glu Leu Ala Glu 
    770                 775                 780                 


Glu Gln Leu Pro Asn Glu Glu Leu Leu Gln Asp Glu Gln Glu Gly Thr 
785                 790                 795                 800 


Asp Gly Glu Arg Pro Arg Arg Arg Ser Arg Gly Gln Arg Arg Arg Ser 
                805                 810                 815     


Asn Arg Arg Glu Arg Gln Arg Asn Ala Asn Gly Glu Leu Ile Asp Gly 
            820                 825                 830         


Gly Glu Glu Glu Glu Gly Ser Glu Glu Gln Pro Gln Gln His Gln Ala 
        835                 840                 845             


Thr Glu Leu Gly Ala Glu Leu Ala Ala Gly Leu Ala Val Thr Ala Ala 
    850                 855                 860                 


Val Ala Ser Ser Asn Ile Ser Ala Asp Ala Glu Ala Gln Ala Asn Gln 
865                 870                 875                 880 


Gln Ala Glu Leu Ala Thr Ala Glu Ile Ala Ala Ala Ala Glu Thr Asp 
                885                 890                 895     


Asn Ser His Ala Ala Gln Pro Val Glu Lys Ala Glu Lys Val Glu Pro 
            900                 905                 910         


Val Glu Ala Val Ala Lys Ala Glu Asp Val Ala Val Ala Pro Val Val 
        915                 920                 925             


Glu Gln Pro Val Ser Glu Pro Val Val Val Ala Glu Val Thr Ala Glu 
    930                 935                 940                 


Pro Val Val Glu Val Ala Pro Gln His Leu Val Glu Glu Ala Pro Ala 
945                 950                 955                 960 


Ala Glu Pro Val Ile Val Ala Glu Ala Pro Val Glu Thr Pro Ala Val 
                965                 970                 975     


Glu Ala Gly Glu Ile Glu Lys Ala Pro Ala Val Val Glu Thr Ala Pro 
            980                 985                 990         


Val Ala Glu Gln Pro Ala Pro Val  Val Glu Ala Gln Pro  Glu Val Val 
        995                 1000                 1005             


Ala Glu  Pro Ala Pro Val Val  Val Glu Pro Ala Pro  Val Glu Ala 
    1010                 1015                 1020             


Glu Ala  Ala Thr Val Met Leu  Ala Asn Gly Arg Ala  Pro Asn Asp 
    1025                 1030                 1035             


Pro Arg  Glu Val Arg Arg Arg  Lys Arg Glu Ala Glu  Ala Ala Ala 
    1040                 1045                 1050             


Lys Ala  Ala Gln Glu Ala Ala  Ala Ala Thr Glu Glu  Pro Ala Leu 
    1055                 1060                 1065             


Glu Ala  Ala Asp Glu His Lys  Pro His His Ser 
    1070                 1075                 


<210>  5
<211>  1563
<212>  DNA
<213>  Bacillus subtilis


<220>
<221>  CDS
<222>  (1)..(1563)
<223>  B. subtilis rny (wild type) encoding RNase Y

<400>  5
atg acc cca att atg atg gtt ctc atc tcc att ttg ctg att cta ctc         48
Met Thr Pro Ile Met Met Val Leu Ile Ser Ile Leu Leu Ile Leu Leu           
1               5                   10                  15                

ggt tta gtt gtt ggc tac ttt gtt cgt aaa acc att gcc gaa gcg aaa         96
Gly Leu Val Val Gly Tyr Phe Val Arg Lys Thr Ile Ala Glu Ala Lys           
            20                  25                  30                    

att gcg ggc gca cgc ggt gca gcc gag caa att ctt gaa gat gcg aag        144
Ile Ala Gly Ala Arg Gly Ala Ala Glu Gln Ile Leu Glu Asp Ala Lys           
        35                  40                  45                        

cgt gat gct gaa gca ctg aaa aaa gaa gct ctg ctt gaa gca aag gat        192
Arg Asp Ala Glu Ala Leu Lys Lys Glu Ala Leu Leu Glu Ala Lys Asp           
    50                  55                  60                            

gaa atc cac aaa ctt cga ata gat gct gaa cag gaa gtt cgt gaa aga        240
Glu Ile His Lys Leu Arg Ile Asp Ala Glu Gln Glu Val Arg Glu Arg           
65                  70                  75                  80            

cga aat gag ctt caa aaa caa gaa aac cgt tta ctc caa aag gag gaa        288
Arg Asn Glu Leu Gln Lys Gln Glu Asn Arg Leu Leu Gln Lys Glu Glu           
                85                  90                  95                

aac ctt gat cgc aaa cat gag gga att gat aaa cgg gaa gcg atg ttg        336
Asn Leu Asp Arg Lys His Glu Gly Ile Asp Lys Arg Glu Ala Met Leu           
            100                 105                 110                   

gag aag aaa gat cat tct ctg aat gaa cga caa caa cat att gaa gag        384
Glu Lys Lys Asp His Ser Leu Asn Glu Arg Gln Gln His Ile Glu Glu           
        115                 120                 125                       

atg gaa agc aaa gtg gat gag atg att cgt atg cag cag tct gag ttg        432
Met Glu Ser Lys Val Asp Glu Met Ile Arg Met Gln Gln Ser Glu Leu           
    130                 135                 140                           

gaa cga att tcg agt ctg act cgt gac gaa gcg aaa caa atc att ctt        480
Glu Arg Ile Ser Ser Leu Thr Arg Asp Glu Ala Lys Gln Ile Ile Leu           
145                 150                 155                 160           

gag cgg gtt gaa aac gag ctt tca cat gac atc gcc atc atg aca aaa        528
Glu Arg Val Glu Asn Glu Leu Ser His Asp Ile Ala Ile Met Thr Lys           
                165                 170                 175               

gaa act gaa aac cgt gcg aaa gaa gag gcg gat aag aaa gcg aaa aac        576
Glu Thr Glu Asn Arg Ala Lys Glu Glu Ala Asp Lys Lys Ala Lys Asn           
            180                 185                 190                   

att ctt tca ctc gcc tta cag cgc tgc gca gcg gac cac gtt gcc gaa        624
Ile Leu Ser Leu Ala Leu Gln Arg Cys Ala Ala Asp His Val Ala Glu           
        195                 200                 205                       

aca acg gta tca gtt gtc aat ctt cca aat gat gag atg aaa gga cgt        672
Thr Thr Val Ser Val Val Asn Leu Pro Asn Asp Glu Met Lys Gly Arg           
    210                 215                 220                           

atc atc gga cgg gaa ggg cgt aac att cgt acg ctt gaa acg ctg aca        720
Ile Ile Gly Arg Glu Gly Arg Asn Ile Arg Thr Leu Glu Thr Leu Thr           
225                 230                 235                 240           

gga att gac ctg att att gat gat acg cct gaa gct gtc att ctt tcc        768
Gly Ile Asp Leu Ile Ile Asp Asp Thr Pro Glu Ala Val Ile Leu Ser           
                245                 250                 255               

gga ttt gat ccg atc aga cgt gag aca gcc aga att gct ctt gat aaa        816
Gly Phe Asp Pro Ile Arg Arg Glu Thr Ala Arg Ile Ala Leu Asp Lys           
            260                 265                 270                   

ctc gtt cag gat ggc cgt att cat ccg gca cgg att gaa gaa atg gtt        864
Leu Val Gln Asp Gly Arg Ile His Pro Ala Arg Ile Glu Glu Met Val           
        275                 280                 285                       

gaa aaa tct cgc cgc gag gtc gat gac tat att cgt gag atg ggt gag        912
Glu Lys Ser Arg Arg Glu Val Asp Asp Tyr Ile Arg Glu Met Gly Glu           
    290                 295                 300                           

caa acg aca ttt gag gtt ggc gtt cat ggc ctc cac cca gat ctc atc        960
Gln Thr Thr Phe Glu Val Gly Val His Gly Leu His Pro Asp Leu Ile           
305                 310                 315                 320           

aag atc ctc ggc cgc tta aag ttc cgt aca agc tac ggt caa aat gtg       1008
Lys Ile Leu Gly Arg Leu Lys Phe Arg Thr Ser Tyr Gly Gln Asn Val           
                325                 330                 335               

ctt aag cat tcc atg gaa gtc gca ttc ttg gcc ggt cta atg gca tcg       1056
Leu Lys His Ser Met Glu Val Ala Phe Leu Ala Gly Leu Met Ala Ser           
            340                 345                 350                   

gag ctt ggt gaa gac gca aag ctt gct aaa cgt gcg ggt ctt ctt cac       1104
Glu Leu Gly Glu Asp Ala Lys Leu Ala Lys Arg Ala Gly Leu Leu His           
        355                 360                 365                       

gac atc ggg aaa gca att gac cat gaa gta gaa gga agc cac gtt gag       1152
Asp Ile Gly Lys Ala Ile Asp His Glu Val Glu Gly Ser His Val Glu           
    370                 375                 380                           

atc ggg gta gag ctt gcg acc aaa tat aaa gag cac cca gtc gtg att       1200
Ile Gly Val Glu Leu Ala Thr Lys Tyr Lys Glu His Pro Val Val Ile           
385                 390                 395                 400           

aac agt att gca tca cac cac ggg gac gag gag ccg act tcc att att       1248
Asn Ser Ile Ala Ser His His Gly Asp Glu Glu Pro Thr Ser Ile Ile           
                405                 410                 415               

gct gta ctg gta gct gca gca gat gcg ctt tcc gct gca aga cct ggc       1296
Ala Val Leu Val Ala Ala Ala Asp Ala Leu Ser Ala Ala Arg Pro Gly           
            420                 425                 430                   

gca aga agt gag acg ctc gag aat tat att cga aga ctt gaa aaa ctt       1344
Ala Arg Ser Glu Thr Leu Glu Asn Tyr Ile Arg Arg Leu Glu Lys Leu           
        435                 440                 445                       

gaa gaa att tct gag tcc tac gaa ggt gtt gaa aaa tca ttt gcc att       1392
Glu Glu Ile Ser Glu Ser Tyr Glu Gly Val Glu Lys Ser Phe Ala Ile           
    450                 455                 460                           

cag gct gga cgc gaa gtg cga att atg gtg aag ccg gat tca att aat       1440
Gln Ala Gly Arg Glu Val Arg Ile Met Val Lys Pro Asp Ser Ile Asn           
465                 470                 475                 480           

gat ctt gag gct cat cga ctg gcg cga gat atc cgc aag cga att gag       1488
Asp Leu Glu Ala His Arg Leu Ala Arg Asp Ile Arg Lys Arg Ile Glu           
                485                 490                 495               

gac gag ctc gat tat cca ggt cat att aag gtg aca gta atc aga gag       1536
Asp Glu Leu Asp Tyr Pro Gly His Ile Lys Val Thr Val Ile Arg Glu           
            500                 505                 510                   

act cga gcc gta gag tat gca aaa taa                                   1563
Thr Arg Ala Val Glu Tyr Ala Lys                                           
        515                 520                                           


<210>  6
<211>  520
<212>  PRT
<213>  Bacillus subtilis

<400>  6

Met Thr Pro Ile Met Met Val Leu Ile Ser Ile Leu Leu Ile Leu Leu 
1               5                   10                  15      


Gly Leu Val Val Gly Tyr Phe Val Arg Lys Thr Ile Ala Glu Ala Lys 
            20                  25                  30          


Ile Ala Gly Ala Arg Gly Ala Ala Glu Gln Ile Leu Glu Asp Ala Lys 
        35                  40                  45              


Arg Asp Ala Glu Ala Leu Lys Lys Glu Ala Leu Leu Glu Ala Lys Asp 
    50                  55                  60                  


Glu Ile His Lys Leu Arg Ile Asp Ala Glu Gln Glu Val Arg Glu Arg 
65                  70                  75                  80  


Arg Asn Glu Leu Gln Lys Gln Glu Asn Arg Leu Leu Gln Lys Glu Glu 
                85                  90                  95      


Asn Leu Asp Arg Lys His Glu Gly Ile Asp Lys Arg Glu Ala Met Leu 
            100                 105                 110         


Glu Lys Lys Asp His Ser Leu Asn Glu Arg Gln Gln His Ile Glu Glu 
        115                 120                 125             


Met Glu Ser Lys Val Asp Glu Met Ile Arg Met Gln Gln Ser Glu Leu 
    130                 135                 140                 


Glu Arg Ile Ser Ser Leu Thr Arg Asp Glu Ala Lys Gln Ile Ile Leu 
145                 150                 155                 160 


Glu Arg Val Glu Asn Glu Leu Ser His Asp Ile Ala Ile Met Thr Lys 
                165                 170                 175     


Glu Thr Glu Asn Arg Ala Lys Glu Glu Ala Asp Lys Lys Ala Lys Asn 
            180                 185                 190         


Ile Leu Ser Leu Ala Leu Gln Arg Cys Ala Ala Asp His Val Ala Glu 
        195                 200                 205             


Thr Thr Val Ser Val Val Asn Leu Pro Asn Asp Glu Met Lys Gly Arg 
    210                 215                 220                 


Ile Ile Gly Arg Glu Gly Arg Asn Ile Arg Thr Leu Glu Thr Leu Thr 
225                 230                 235                 240 


Gly Ile Asp Leu Ile Ile Asp Asp Thr Pro Glu Ala Val Ile Leu Ser 
                245                 250                 255     


Gly Phe Asp Pro Ile Arg Arg Glu Thr Ala Arg Ile Ala Leu Asp Lys 
            260                 265                 270         


Leu Val Gln Asp Gly Arg Ile His Pro Ala Arg Ile Glu Glu Met Val 
        275                 280                 285             


Glu Lys Ser Arg Arg Glu Val Asp Asp Tyr Ile Arg Glu Met Gly Glu 
    290                 295                 300                 


Gln Thr Thr Phe Glu Val Gly Val His Gly Leu His Pro Asp Leu Ile 
305                 310                 315                 320 


Lys Ile Leu Gly Arg Leu Lys Phe Arg Thr Ser Tyr Gly Gln Asn Val 
                325                 330                 335     


Leu Lys His Ser Met Glu Val Ala Phe Leu Ala Gly Leu Met Ala Ser 
            340                 345                 350         


Glu Leu Gly Glu Asp Ala Lys Leu Ala Lys Arg Ala Gly Leu Leu His 
        355                 360                 365             


Asp Ile Gly Lys Ala Ile Asp His Glu Val Glu Gly Ser His Val Glu 
    370                 375                 380                 


Ile Gly Val Glu Leu Ala Thr Lys Tyr Lys Glu His Pro Val Val Ile 
385                 390                 395                 400 


Asn Ser Ile Ala Ser His His Gly Asp Glu Glu Pro Thr Ser Ile Ile 
                405                 410                 415     


Ala Val Leu Val Ala Ala Ala Asp Ala Leu Ser Ala Ala Arg Pro Gly 
            420                 425                 430         


Ala Arg Ser Glu Thr Leu Glu Asn Tyr Ile Arg Arg Leu Glu Lys Leu 
        435                 440                 445             


Glu Glu Ile Ser Glu Ser Tyr Glu Gly Val Glu Lys Ser Phe Ala Ile 
    450                 455                 460                 


Gln Ala Gly Arg Glu Val Arg Ile Met Val Lys Pro Asp Ser Ile Asn 
465                 470                 475                 480 


Asp Leu Glu Ala His Arg Leu Ala Arg Asp Ile Arg Lys Arg Ile Glu 
                485                 490                 495     


Asp Glu Leu Asp Tyr Pro Gly His Ile Lys Val Thr Val Ile Arg Glu 
            500                 505                 510         


Thr Arg Ala Val Glu Tyr Ala Lys 
        515                 520 


<210>  7
<211>  1560
<212>  DNA
<213>  Bacillus licheniformis


<220>
<221>  CDS
<222>  (1)..(1560)
<223>  Bacillus licheniformis rny encoding RNase Y

<400>  7
atg agt cct tta gca att ctc atc tcc att ttg ctg agc cta ttc tgt         48
Met Ser Pro Leu Ala Ile Leu Ile Ser Ile Leu Leu Ser Leu Phe Cys           
1               5                   10                  15                

tta gtt gtt ggc tac tat gtt cgt aaa atc att gcc gaa gca aaa att         96
Leu Val Val Gly Tyr Tyr Val Arg Lys Ile Ile Ala Glu Ala Lys Ile           
            20                  25                  30                    

tca ggt gcg cga aat gca gcc gaa caa att ctt gga gac gca aag cgg        144
Ser Gly Ala Arg Asn Ala Ala Glu Gln Ile Leu Gly Asp Ala Lys Arg           
        35                  40                  45                        

gat gct gaa gcg ttg aaa aaa gaa gcc ctt ctt gaa gca aag gac gag        192
Asp Ala Glu Ala Leu Lys Lys Glu Ala Leu Leu Glu Ala Lys Asp Glu           
    50                  55                  60                            

att cat acg ctt cgg ata gaa gct gaa caa gaa gtt cgt gaa aga cga        240
Ile His Thr Leu Arg Ile Glu Ala Glu Gln Glu Val Arg Glu Arg Arg           
65                  70                  75                  80            

aat gag ctt caa aaa caa gaa aac cgt tta ctt caa aag gaa gag aac        288
Asn Glu Leu Gln Lys Gln Glu Asn Arg Leu Leu Gln Lys Glu Glu Asn           
                85                  90                  95                

ctt gac cga aaa gat gaa tca tta gat aaa cgg gaa gcg atg ttg gag        336
Leu Asp Arg Lys Asp Glu Ser Leu Asp Lys Arg Glu Ala Met Leu Glu           
            100                 105                 110                   

aag aaa gat cat tct ctg aat gaa cga caa caa cat att gaa gag atg        384
Lys Lys Asp His Ser Leu Asn Glu Arg Gln Gln His Ile Glu Glu Met           
        115                 120                 125                       

gaa agc aaa gtg gat gaa atg att cgt atg cag cag tca gag ttg gag        432
Glu Ser Lys Val Asp Glu Met Ile Arg Met Gln Gln Ser Glu Leu Glu           
    130                 135                 140                           

cgt att tca agt ctg acg cga gat gaa gcg aag caa atc att ctg gaa        480
Arg Ile Ser Ser Leu Thr Arg Asp Glu Ala Lys Gln Ile Ile Leu Glu           
145                 150                 155                 160           

cgg gtt gaa aac gag ctt tcc cat gac atc gcg atc atg atg aaa gaa        528
Arg Val Glu Asn Glu Leu Ser His Asp Ile Ala Ile Met Met Lys Glu           
                165                 170                 175               

agt gaa aat cga gcg aaa gaa gag gct gat aaa aag gcg aaa aat att        576
Ser Glu Asn Arg Ala Lys Glu Glu Ala Asp Lys Lys Ala Lys Asn Ile           
            180                 185                 190                   

ctt tca ttg gct tta cag cgc tgt gcg gca gat cat gtg gcc gag aca        624
Leu Ser Leu Ala Leu Gln Arg Cys Ala Ala Asp His Val Ala Glu Thr           
        195                 200                 205                       

acg gtt tca gtt gtc aac ctt cca aat gat gag atg aaa ggc cgc att        672
Thr Val Ser Val Val Asn Leu Pro Asn Asp Glu Met Lys Gly Arg Ile           
    210                 215                 220                           

atc gga cgt gaa gga cgt aat atc cgt aca ttg gaa aca ttg acg ggg        720
Ile Gly Arg Glu Gly Arg Asn Ile Arg Thr Leu Glu Thr Leu Thr Gly           
225                 230                 235                 240           

atc gat ctg atc atc gac gat acg ccg gaa gcc gtt atc ctt tca gga        768
Ile Asp Leu Ile Ile Asp Asp Thr Pro Glu Ala Val Ile Leu Ser Gly           
                245                 250                 255               

ttt gat ccg atc agg cgt gaa aca gcc agg atc gct ctt gat aaa ctc        816
Phe Asp Pro Ile Arg Arg Glu Thr Ala Arg Ile Ala Leu Asp Lys Leu           
            260                 265                 270                   

gtt cag gat ggc cgc att cat cct gcc aga atc gaa gaa atg gtt gag        864
Val Gln Asp Gly Arg Ile His Pro Ala Arg Ile Glu Glu Met Val Glu           
        275                 280                 285                       

aaa tcc cgc cgt gaa gtg gat gat tac atc cgc gaa atg ggt gaa cag        912
Lys Ser Arg Arg Glu Val Asp Asp Tyr Ile Arg Glu Met Gly Glu Gln           
    290                 295                 300                           

acg acg ttt gaa gtt gga gtt cac ggt ctt cat ccc gat tta atc aaa        960
Thr Thr Phe Glu Val Gly Val His Gly Leu His Pro Asp Leu Ile Lys           
305                 310                 315                 320           

atc ctc ggc cgc ttg aag ttc aga acg agc tac gga cag aat gta ctg       1008
Ile Leu Gly Arg Leu Lys Phe Arg Thr Ser Tyr Gly Gln Asn Val Leu           
                325                 330                 335               

aaa cat tca atg gaa gtg gcg ttt ctg aca ggc ttg atg gct tca gag       1056
Lys His Ser Met Glu Val Ala Phe Leu Thr Gly Leu Met Ala Ser Glu           
            340                 345                 350                   

ctc gga gaa gac gta acg ctc gca aaa agg gca gga ctt ctt cat gat       1104
Leu Gly Glu Asp Val Thr Leu Ala Lys Arg Ala Gly Leu Leu His Asp           
        355                 360                 365                       

atc ggg aaa gcg att gac cat gag gtg gaa gga agc cac gtt gaa atc       1152
Ile Gly Lys Ala Ile Asp His Glu Val Glu Gly Ser His Val Glu Ile           
    370                 375                 380                           

ggt gtg gaa ctg gcc acc aag tat aaa gag cat cca gtt gtc atc aac       1200
Gly Val Glu Leu Ala Thr Lys Tyr Lys Glu His Pro Val Val Ile Asn           
385                 390                 395                 400           

agt atc gca tct cac cac gga gat cag gag ccg act tcc atc atc gcc       1248
Ser Ile Ala Ser His His Gly Asp Gln Glu Pro Thr Ser Ile Ile Ala           
                405                 410                 415               

gtg ctc gtg gcc gca gcc gat gcg ctg tct gcc gca aga cca ggc gca       1296
Val Leu Val Ala Ala Ala Asp Ala Leu Ser Ala Ala Arg Pro Gly Ala           
            420                 425                 430                   

aga agc gaa acg ctc gaa aac tat att cgc agg ctt gaa aaa ctg gaa       1344
Arg Ser Glu Thr Leu Glu Asn Tyr Ile Arg Arg Leu Glu Lys Leu Glu           
        435                 440                 445                       

gaa atc tcc gaa tca tac gaa ggt gtt gaa aaa tca ttc gcg ata caa       1392
Glu Ile Ser Glu Ser Tyr Glu Gly Val Glu Lys Ser Phe Ala Ile Gln           
    450                 455                 460                           

gcg gga cga gag gtg cgg atc atg gtc aaa cct gac tca atc aat gat       1440
Ala Gly Arg Glu Val Arg Ile Met Val Lys Pro Asp Ser Ile Asn Asp           
465                 470                 475                 480           

ctt gaa gcc cat cgt ttg gcg cgt gat att cga aaa cga att gag gac       1488
Leu Glu Ala His Arg Leu Ala Arg Asp Ile Arg Lys Arg Ile Glu Asp           
                485                 490                 495               

gag ctt gac tac cct gga cac atc aag gta acc gta atc agg gaa acg       1536
Glu Leu Asp Tyr Pro Gly His Ile Lys Val Thr Val Ile Arg Glu Thr           
            500                 505                 510                   

aga gcc gtt gaa tac gca aaa taa                                       1560
Arg Ala Val Glu Tyr Ala Lys                                               
        515                                                               


<210>  8
<211>  519
<212>  PRT
<213>  Bacillus licheniformis

<400>  8

Met Ser Pro Leu Ala Ile Leu Ile Ser Ile Leu Leu Ser Leu Phe Cys 
1               5                   10                  15      


Leu Val Val Gly Tyr Tyr Val Arg Lys Ile Ile Ala Glu Ala Lys Ile 
            20                  25                  30          


Ser Gly Ala Arg Asn Ala Ala Glu Gln Ile Leu Gly Asp Ala Lys Arg 
        35                  40                  45              


Asp Ala Glu Ala Leu Lys Lys Glu Ala Leu Leu Glu Ala Lys Asp Glu 
    50                  55                  60                  


Ile His Thr Leu Arg Ile Glu Ala Glu Gln Glu Val Arg Glu Arg Arg 
65                  70                  75                  80  


Asn Glu Leu Gln Lys Gln Glu Asn Arg Leu Leu Gln Lys Glu Glu Asn 
                85                  90                  95      


Leu Asp Arg Lys Asp Glu Ser Leu Asp Lys Arg Glu Ala Met Leu Glu 
            100                 105                 110         


Lys Lys Asp His Ser Leu Asn Glu Arg Gln Gln His Ile Glu Glu Met 
        115                 120                 125             


Glu Ser Lys Val Asp Glu Met Ile Arg Met Gln Gln Ser Glu Leu Glu 
    130                 135                 140                 


Arg Ile Ser Ser Leu Thr Arg Asp Glu Ala Lys Gln Ile Ile Leu Glu 
145                 150                 155                 160 


Arg Val Glu Asn Glu Leu Ser His Asp Ile Ala Ile Met Met Lys Glu 
                165                 170                 175     


Ser Glu Asn Arg Ala Lys Glu Glu Ala Asp Lys Lys Ala Lys Asn Ile 
            180                 185                 190         


Leu Ser Leu Ala Leu Gln Arg Cys Ala Ala Asp His Val Ala Glu Thr 
        195                 200                 205             


Thr Val Ser Val Val Asn Leu Pro Asn Asp Glu Met Lys Gly Arg Ile 
    210                 215                 220                 


Ile Gly Arg Glu Gly Arg Asn Ile Arg Thr Leu Glu Thr Leu Thr Gly 
225                 230                 235                 240 


Ile Asp Leu Ile Ile Asp Asp Thr Pro Glu Ala Val Ile Leu Ser Gly 
                245                 250                 255     


Phe Asp Pro Ile Arg Arg Glu Thr Ala Arg Ile Ala Leu Asp Lys Leu 
            260                 265                 270         


Val Gln Asp Gly Arg Ile His Pro Ala Arg Ile Glu Glu Met Val Glu 
        275                 280                 285             


Lys Ser Arg Arg Glu Val Asp Asp Tyr Ile Arg Glu Met Gly Glu Gln 
    290                 295                 300                 


Thr Thr Phe Glu Val Gly Val His Gly Leu His Pro Asp Leu Ile Lys 
305                 310                 315                 320 


Ile Leu Gly Arg Leu Lys Phe Arg Thr Ser Tyr Gly Gln Asn Val Leu 
                325                 330                 335     


Lys His Ser Met Glu Val Ala Phe Leu Thr Gly Leu Met Ala Ser Glu 
            340                 345                 350         


Leu Gly Glu Asp Val Thr Leu Ala Lys Arg Ala Gly Leu Leu His Asp 
        355                 360                 365             


Ile Gly Lys Ala Ile Asp His Glu Val Glu Gly Ser His Val Glu Ile 
    370                 375                 380                 


Gly Val Glu Leu Ala Thr Lys Tyr Lys Glu His Pro Val Val Ile Asn 
385                 390                 395                 400 


Ser Ile Ala Ser His His Gly Asp Gln Glu Pro Thr Ser Ile Ile Ala 
                405                 410                 415     


Val Leu Val Ala Ala Ala Asp Ala Leu Ser Ala Ala Arg Pro Gly Ala 
            420                 425                 430         


Arg Ser Glu Thr Leu Glu Asn Tyr Ile Arg Arg Leu Glu Lys Leu Glu 
        435                 440                 445             


Glu Ile Ser Glu Ser Tyr Glu Gly Val Glu Lys Ser Phe Ala Ile Gln 
    450                 455                 460                 


Ala Gly Arg Glu Val Arg Ile Met Val Lys Pro Asp Ser Ile Asn Asp 
465                 470                 475                 480 


Leu Glu Ala His Arg Leu Ala Arg Asp Ile Arg Lys Arg Ile Glu Asp 
                485                 490                 495     


Glu Leu Asp Tyr Pro Gly His Ile Lys Val Thr Val Ile Arg Glu Thr 
            500                 505                 510         


Arg Ala Val Glu Tyr Ala Lys 
        515                 


<210>  9
<211>  2514
<212>  DNA
<213>  Saccharomyces cereviciae


<220>
<221>  CDS
<222>  (1)..(2514)
<223>  CCR4

<400>  9
atg aac gac cct tct tta cta ggc tac cct aat gtg ggc ccg cag cag         48
Met Asn Asp Pro Ser Leu Leu Gly Tyr Pro Asn Val Gly Pro Gln Gln           
1               5                   10                  15                

caa cag caa cag cag caa cag cag cat gcc ggg cta cta gga aag ggt         96
Gln Gln Gln Gln Gln Gln Gln Gln His Ala Gly Leu Leu Gly Lys Gly           
            20                  25                  30                    

aca cca aac gct cta cag cag cag cta cac atg aac cag ctc acc ggg        144
Thr Pro Asn Ala Leu Gln Gln Gln Leu His Met Asn Gln Leu Thr Gly           
        35                  40                  45                        

ata cct cca ccg gga ctc atg aac aac agc gat gta cac act tcc agc        192
Ile Pro Pro Pro Gly Leu Met Asn Asn Ser Asp Val His Thr Ser Ser           
    50                  55                  60                            

aac aac aac tcg cgc cag ttg ctc gac caa ttg gct aat ggg aac gcg        240
Asn Asn Asn Ser Arg Gln Leu Leu Asp Gln Leu Ala Asn Gly Asn Ala           
65                  70                  75                  80            

aac atg ctc aat atg aac atg gat aac aac aat aac aac aat aac aac        288
Asn Met Leu Asn Met Asn Met Asp Asn Asn Asn Asn Asn Asn Asn Asn           
                85                  90                  95                

aac aac aac aac aac aac aac ggc ggt gga agc ggg gtg atg atg aat        336
Asn Asn Asn Asn Asn Asn Asn Gly Gly Gly Ser Gly Val Met Met Asn           
            100                 105                 110                   

gcc tcc aca gcc gca gta aat tct ata ggg atg gtt cct acc gtt ggt        384
Ala Ser Thr Ala Ala Val Asn Ser Ile Gly Met Val Pro Thr Val Gly           
        115                 120                 125                       

acg cct gtg aac atc aat gtg aac gcc agt aac cct tta tta cac ccg        432
Thr Pro Val Asn Ile Asn Val Asn Ala Ser Asn Pro Leu Leu His Pro           
    130                 135                 140                           

cac ttg gat gac cct tct tta cta aac aat ccg atc tgg aag ctt caa        480
His Leu Asp Asp Pro Ser Leu Leu Asn Asn Pro Ile Trp Lys Leu Gln           
145                 150                 155                 160           

ttg cat ctg gct gca gtg tcc gca caa tct ctg ggg caa ccc aac att        528
Leu His Leu Ala Ala Val Ser Ala Gln Ser Leu Gly Gln Pro Asn Ile           
                165                 170                 175               

tat gct aga caa aac gct atg aag aag tat ttg gct aca caa cag gct        576
Tyr Ala Arg Gln Asn Ala Met Lys Lys Tyr Leu Ala Thr Gln Gln Ala           
            180                 185                 190                   

cag caa gct cag caa caa gcg cag caa cag gca cag cag cag gtc ccg        624
Gln Gln Ala Gln Gln Gln Ala Gln Gln Gln Ala Gln Gln Gln Val Pro           
        195                 200                 205                       

ggc cca ttt ggc ccc gga cct cag gct gca cca cca gct ttg cag ccc        672
Gly Pro Phe Gly Pro Gly Pro Gln Ala Ala Pro Pro Ala Leu Gln Pro           
    210                 215                 220                           

acc gat ttc cag caa tct cac att gca gaa gcc tcc aaa tca ctg gta        720
Thr Asp Phe Gln Gln Ser His Ile Ala Glu Ala Ser Lys Ser Leu Val           
225                 230                 235                 240           

gac tgc aca aag caa gcc ttg atg gaa atg gcc gac act ctc acc gac        768
Asp Cys Thr Lys Gln Ala Leu Met Glu Met Ala Asp Thr Leu Thr Asp           
                245                 250                 255               

agc aag aca gca aag aaa caa caa cct acg gga gat agc act ccc tca        816
Ser Lys Thr Ala Lys Lys Gln Gln Pro Thr Gly Asp Ser Thr Pro Ser           
            260                 265                 270                   

ggc acg gca act aac agt gca gtt tct aca cca ttg act ccc aag ata        864
Gly Thr Ala Thr Asn Ser Ala Val Ser Thr Pro Leu Thr Pro Lys Ile           
        275                 280                 285                       

gag ctg ttt gct aat ggc aag gac gaa gcc aac cag gcg ctc tta caa        912
Glu Leu Phe Ala Asn Gly Lys Asp Glu Ala Asn Gln Ala Leu Leu Gln           
    290                 295                 300                           

cac aag aaa ctg tct cag tac agc atc gac gaa gat gac gac att gaa        960
His Lys Lys Leu Ser Gln Tyr Ser Ile Asp Glu Asp Asp Asp Ile Glu           
305                 310                 315                 320           

aac aga atg gtc atg ccc aag gac tcg aaa tac gac gac caa tta tgg       1008
Asn Arg Met Val Met Pro Lys Asp Ser Lys Tyr Asp Asp Gln Leu Trp           
                325                 330                 335               

cac gcg cta gat ttg tcc aac ttg caa atc ttc aat atc agc gcc aac       1056
His Ala Leu Asp Leu Ser Asn Leu Gln Ile Phe Asn Ile Ser Ala Asn           
            340                 345                 350                   

atc ttc aag tac gat ttt cta acg aga cta tat ttg aat ggc aat agc       1104
Ile Phe Lys Tyr Asp Phe Leu Thr Arg Leu Tyr Leu Asn Gly Asn Ser           
        355                 360                 365                       

ctc acg gaa ctg cca gcg gag atc aag aac cta agc aac cta cgc gtt       1152
Leu Thr Glu Leu Pro Ala Glu Ile Lys Asn Leu Ser Asn Leu Arg Val           
    370                 375                 380                           

ttg gac ctg tcg cat aat agg tta aca tct cta cca gcg gaa cta ggc       1200
Leu Asp Leu Ser His Asn Arg Leu Thr Ser Leu Pro Ala Glu Leu Gly           
385                 390                 395                 400           

tca tgt ttc caa ttg aaa tac ttc tac ttt ttt gat aac atg gtc acc       1248
Ser Cys Phe Gln Leu Lys Tyr Phe Tyr Phe Phe Asp Asn Met Val Thr           
                405                 410                 415               

aca tta cca tgg gag ttt ggg aac ctg tgt aac ctt cag ttt ctt ggt       1296
Thr Leu Pro Trp Glu Phe Gly Asn Leu Cys Asn Leu Gln Phe Leu Gly           
            420                 425                 430                   

gta gaa gga aac ccc tta gaa aaa cag ttt tta aag atc ctt aca gaa       1344
Val Glu Gly Asn Pro Leu Glu Lys Gln Phe Leu Lys Ile Leu Thr Glu           
        435                 440                 445                       

aaa tct gtc acg gga ttg att ttc tac cta agg gac aac aga cca gag       1392
Lys Ser Val Thr Gly Leu Ile Phe Tyr Leu Arg Asp Asn Arg Pro Glu           
    450                 455                 460                           

att ccc tta ccg cat gaa cgt agg ttc atc gaa atc aat acc gat ggg       1440
Ile Pro Leu Pro His Glu Arg Arg Phe Ile Glu Ile Asn Thr Asp Gly           
465                 470                 475                 480           

gaa cca cag agg gag tac gat tct ttg cag caa tcc act gag cat ctg       1488
Glu Pro Gln Arg Glu Tyr Asp Ser Leu Gln Gln Ser Thr Glu His Leu           
                485                 490                 495               

gcc acc gat tta gcc aag agg acg ttc acc gtc tta tcc tac aac acc       1536
Ala Thr Asp Leu Ala Lys Arg Thr Phe Thr Val Leu Ser Tyr Asn Thr           
            500                 505                 510                   

tta tgt caa cac tat gcc acc cca aaa atg tac cgt tac aca ccg tcg       1584
Leu Cys Gln His Tyr Ala Thr Pro Lys Met Tyr Arg Tyr Thr Pro Ser           
        515                 520                 525                       

tgg gcg tta agt tgg gat tac agg cgc aat aaa tta aag gag cag att       1632
Trp Ala Leu Ser Trp Asp Tyr Arg Arg Asn Lys Leu Lys Glu Gln Ile           
    530                 535                 540                           

ctc tcg tac gac agt gat ctg ttg tgt tta caa gaa gtg gag tct aag       1680
Leu Ser Tyr Asp Ser Asp Leu Leu Cys Leu Gln Glu Val Glu Ser Lys           
545                 550                 555                 560           

act ttt gaa gag tat tgg gtg ccc cta ttg gac aag cac ggt tat aca       1728
Thr Phe Glu Glu Tyr Trp Val Pro Leu Leu Asp Lys His Gly Tyr Thr           
                565                 570                 575               

ggc atc ttc cat gca aag gca aga gcc aag acc atg cat tcc aag gac       1776
Gly Ile Phe His Ala Lys Ala Arg Ala Lys Thr Met His Ser Lys Asp           
            580                 585                 590                   

tcc aag aaa gtg gac ggg tgt tgc att ttt ttc aaa agg gac caa ttc       1824
Ser Lys Lys Val Asp Gly Cys Cys Ile Phe Phe Lys Arg Asp Gln Phe           
        595                 600                 605                       

aag ttg atc acc aaa gac gcc atg gat ttc agc ggt gct tgg atg aaa       1872
Lys Leu Ile Thr Lys Asp Ala Met Asp Phe Ser Gly Ala Trp Met Lys           
    610                 615                 620                           

cac aag aag ttc caa aga act gaa gat tat tta aac cgt gca atg aac       1920
His Lys Lys Phe Gln Arg Thr Glu Asp Tyr Leu Asn Arg Ala Met Asn           
625                 630                 635                 640           

aaa gac aac gtt gca ctg ttc tta aag cta caa cac att cct agt ggc       1968
Lys Asp Asn Val Ala Leu Phe Leu Lys Leu Gln His Ile Pro Ser Gly           
                645                 650                 655               

gac acc ata tgg gcg gtc acc acg cat ttg cac tgg gat cca aaa ttt       2016
Asp Thr Ile Trp Ala Val Thr Thr His Leu His Trp Asp Pro Lys Phe           
            660                 665                 670                   

aat gat gtc aag aca ttc caa gta ggt gtc ctg tta gat cat ctg gaa       2064
Asn Asp Val Lys Thr Phe Gln Val Gly Val Leu Leu Asp His Leu Glu           
        675                 680                 685                       

acg ctg cta aag gag gag aca tcg cac aat ttt aga cag gac att aag       2112
Thr Leu Leu Lys Glu Glu Thr Ser His Asn Phe Arg Gln Asp Ile Lys           
    690                 695                 700                           

aaa ttt cct gtg ctc att tgt ggt gac ttc aat tca tac atc aac tcc       2160
Lys Phe Pro Val Leu Ile Cys Gly Asp Phe Asn Ser Tyr Ile Asn Ser           
705                 710                 715                 720           

gcc gta tac gaa ttg ata aat aca ggc cgt gtc caa ata cat caa gag       2208
Ala Val Tyr Glu Leu Ile Asn Thr Gly Arg Val Gln Ile His Gln Glu           
                725                 730                 735               

gga aat ggt aga gat ttc ggt tac atg tcg gag aaa aat ttc tca cat       2256
Gly Asn Gly Arg Asp Phe Gly Tyr Met Ser Glu Lys Asn Phe Ser His           
            740                 745                 750                   

aac ttg gct ctc aaa tca agt tat aat tgt atc gga gaa cta cca ttc       2304
Asn Leu Ala Leu Lys Ser Ser Tyr Asn Cys Ile Gly Glu Leu Pro Phe           
        755                 760                 765                       

acc aat ttc aca ccg tca ttc aca gat gtt atc gac tat ata tgg ttt       2352
Thr Asn Phe Thr Pro Ser Phe Thr Asp Val Ile Asp Tyr Ile Trp Phe           
    770                 775                 780                           

tct aca cat gct cta agg gtg cgt ggg cta ttg ggt gaa gtg gac cct       2400
Ser Thr His Ala Leu Arg Val Arg Gly Leu Leu Gly Glu Val Asp Pro           
785                 790                 795                 800           

gaa tac gtg agt aag ttt atc ggg ttc ccc aac gac aaa ttc ccc agt       2448
Glu Tyr Val Ser Lys Phe Ile Gly Phe Pro Asn Asp Lys Phe Pro Ser           
                805                 810                 815               

gac cat ata cca ttg tta gca aga ttt gaa ttt atg aag aca aac aca       2496
Asp His Ile Pro Leu Leu Ala Arg Phe Glu Phe Met Lys Thr Asn Thr           
            820                 825                 830                   

ggc agt aag aaa gta taa                                               2514
Gly Ser Lys Lys Val                                                       
        835                                                               


<210>  10
<211>  837
<212>  PRT
<213>  Saccharomyces cereviciae

<400>  10

Met Asn Asp Pro Ser Leu Leu Gly Tyr Pro Asn Val Gly Pro Gln Gln 
1               5                   10                  15      


Gln Gln Gln Gln Gln Gln Gln Gln His Ala Gly Leu Leu Gly Lys Gly 
            20                  25                  30          


Thr Pro Asn Ala Leu Gln Gln Gln Leu His Met Asn Gln Leu Thr Gly 
        35                  40                  45              


Ile Pro Pro Pro Gly Leu Met Asn Asn Ser Asp Val His Thr Ser Ser 
    50                  55                  60                  


Asn Asn Asn Ser Arg Gln Leu Leu Asp Gln Leu Ala Asn Gly Asn Ala 
65                  70                  75                  80  


Asn Met Leu Asn Met Asn Met Asp Asn Asn Asn Asn Asn Asn Asn Asn 
                85                  90                  95      


Asn Asn Asn Asn Asn Asn Asn Gly Gly Gly Ser Gly Val Met Met Asn 
            100                 105                 110         


Ala Ser Thr Ala Ala Val Asn Ser Ile Gly Met Val Pro Thr Val Gly 
        115                 120                 125             


Thr Pro Val Asn Ile Asn Val Asn Ala Ser Asn Pro Leu Leu His Pro 
    130                 135                 140                 


His Leu Asp Asp Pro Ser Leu Leu Asn Asn Pro Ile Trp Lys Leu Gln 
145                 150                 155                 160 


Leu His Leu Ala Ala Val Ser Ala Gln Ser Leu Gly Gln Pro Asn Ile 
                165                 170                 175     


Tyr Ala Arg Gln Asn Ala Met Lys Lys Tyr Leu Ala Thr Gln Gln Ala 
            180                 185                 190         


Gln Gln Ala Gln Gln Gln Ala Gln Gln Gln Ala Gln Gln Gln Val Pro 
        195                 200                 205             


Gly Pro Phe Gly Pro Gly Pro Gln Ala Ala Pro Pro Ala Leu Gln Pro 
    210                 215                 220                 


Thr Asp Phe Gln Gln Ser His Ile Ala Glu Ala Ser Lys Ser Leu Val 
225                 230                 235                 240 


Asp Cys Thr Lys Gln Ala Leu Met Glu Met Ala Asp Thr Leu Thr Asp 
                245                 250                 255     


Ser Lys Thr Ala Lys Lys Gln Gln Pro Thr Gly Asp Ser Thr Pro Ser 
            260                 265                 270         


Gly Thr Ala Thr Asn Ser Ala Val Ser Thr Pro Leu Thr Pro Lys Ile 
        275                 280                 285             


Glu Leu Phe Ala Asn Gly Lys Asp Glu Ala Asn Gln Ala Leu Leu Gln 
    290                 295                 300                 


His Lys Lys Leu Ser Gln Tyr Ser Ile Asp Glu Asp Asp Asp Ile Glu 
305                 310                 315                 320 


Asn Arg Met Val Met Pro Lys Asp Ser Lys Tyr Asp Asp Gln Leu Trp 
                325                 330                 335     


His Ala Leu Asp Leu Ser Asn Leu Gln Ile Phe Asn Ile Ser Ala Asn 
            340                 345                 350         


Ile Phe Lys Tyr Asp Phe Leu Thr Arg Leu Tyr Leu Asn Gly Asn Ser 
        355                 360                 365             


Leu Thr Glu Leu Pro Ala Glu Ile Lys Asn Leu Ser Asn Leu Arg Val 
    370                 375                 380                 


Leu Asp Leu Ser His Asn Arg Leu Thr Ser Leu Pro Ala Glu Leu Gly 
385                 390                 395                 400 


Ser Cys Phe Gln Leu Lys Tyr Phe Tyr Phe Phe Asp Asn Met Val Thr 
                405                 410                 415     


Thr Leu Pro Trp Glu Phe Gly Asn Leu Cys Asn Leu Gln Phe Leu Gly 
            420                 425                 430         


Val Glu Gly Asn Pro Leu Glu Lys Gln Phe Leu Lys Ile Leu Thr Glu 
        435                 440                 445             


Lys Ser Val Thr Gly Leu Ile Phe Tyr Leu Arg Asp Asn Arg Pro Glu 
    450                 455                 460                 


Ile Pro Leu Pro His Glu Arg Arg Phe Ile Glu Ile Asn Thr Asp Gly 
465                 470                 475                 480 


Glu Pro Gln Arg Glu Tyr Asp Ser Leu Gln Gln Ser Thr Glu His Leu 
                485                 490                 495     


Ala Thr Asp Leu Ala Lys Arg Thr Phe Thr Val Leu Ser Tyr Asn Thr 
            500                 505                 510         


Leu Cys Gln His Tyr Ala Thr Pro Lys Met Tyr Arg Tyr Thr Pro Ser 
        515                 520                 525             


Trp Ala Leu Ser Trp Asp Tyr Arg Arg Asn Lys Leu Lys Glu Gln Ile 
    530                 535                 540                 


Leu Ser Tyr Asp Ser Asp Leu Leu Cys Leu Gln Glu Val Glu Ser Lys 
545                 550                 555                 560 


Thr Phe Glu Glu Tyr Trp Val Pro Leu Leu Asp Lys His Gly Tyr Thr 
                565                 570                 575     


Gly Ile Phe His Ala Lys Ala Arg Ala Lys Thr Met His Ser Lys Asp 
            580                 585                 590         


Ser Lys Lys Val Asp Gly Cys Cys Ile Phe Phe Lys Arg Asp Gln Phe 
        595                 600                 605             


Lys Leu Ile Thr Lys Asp Ala Met Asp Phe Ser Gly Ala Trp Met Lys 
    610                 615                 620                 


His Lys Lys Phe Gln Arg Thr Glu Asp Tyr Leu Asn Arg Ala Met Asn 
625                 630                 635                 640 


Lys Asp Asn Val Ala Leu Phe Leu Lys Leu Gln His Ile Pro Ser Gly 
                645                 650                 655     


Asp Thr Ile Trp Ala Val Thr Thr His Leu His Trp Asp Pro Lys Phe 
            660                 665                 670         


Asn Asp Val Lys Thr Phe Gln Val Gly Val Leu Leu Asp His Leu Glu 
        675                 680                 685             


Thr Leu Leu Lys Glu Glu Thr Ser His Asn Phe Arg Gln Asp Ile Lys 
    690                 695                 700                 


Lys Phe Pro Val Leu Ile Cys Gly Asp Phe Asn Ser Tyr Ile Asn Ser 
705                 710                 715                 720 


Ala Val Tyr Glu Leu Ile Asn Thr Gly Arg Val Gln Ile His Gln Glu 
                725                 730                 735     


Gly Asn Gly Arg Asp Phe Gly Tyr Met Ser Glu Lys Asn Phe Ser His 
            740                 745                 750         


Asn Leu Ala Leu Lys Ser Ser Tyr Asn Cys Ile Gly Glu Leu Pro Phe 
        755                 760                 765             


Thr Asn Phe Thr Pro Ser Phe Thr Asp Val Ile Asp Tyr Ile Trp Phe 
    770                 775                 780                 


Ser Thr His Ala Leu Arg Val Arg Gly Leu Leu Gly Glu Val Asp Pro 
785                 790                 795                 800 


Glu Tyr Val Ser Lys Phe Ile Gly Phe Pro Asn Asp Lys Phe Pro Ser 
                805                 810                 815     


Asp His Ile Pro Leu Leu Ala Arg Phe Glu Phe Met Lys Thr Asn Thr 
            820                 825                 830         


Gly Ser Lys Lys Val 
        835         


<210>  11
<211>  3348
<212>  DNA
<213>  Saccharomyces cereviciae


<220>
<221>  CDS
<222>  (1)..(3348)
<223>  Pan2

<400>  11
atg aat aat tgg caa cat ttc ttc aac aat cca gtt gat ctt tcg gaa         48
Met Asn Asn Trp Gln His Phe Phe Asn Asn Pro Val Asp Leu Ser Glu           
1               5                   10                  15                

cat ttg aag aag cca tac ttt cgc ttc gat aat agg gat aag gaa att         96
His Leu Lys Lys Pro Tyr Phe Arg Phe Asp Asn Arg Asp Lys Glu Ile           
            20                  25                  30                    

aca gcg att agc ttc gat gag aag gca aac tta att tgg agt gga gac        144
Thr Ala Ile Ser Phe Asp Glu Lys Ala Asn Leu Ile Trp Ser Gly Asp           
        35                  40                  45                        

agc tat ggt tgc att tcg tca tat gat cca act ttt caa ctt tat aca        192
Ser Tyr Gly Cys Ile Ser Ser Tyr Asp Pro Thr Phe Gln Leu Tyr Thr           
    50                  55                  60                            

aga tat agg ggc cac ata ggt gga aat tcc gtg aag gat att ctc agt        240
Arg Tyr Arg Gly His Ile Gly Gly Asn Ser Val Lys Asp Ile Leu Ser           
65                  70                  75                  80            

cat cgg gat ggt att tta tct att agt gaa gat tcc tta cac ttt gct        288
His Arg Asp Gly Ile Leu Ser Ile Ser Glu Asp Ser Leu His Phe Ala           
                85                  90                  95                

aat aga aga ggt gtt act aaa ttg aac ctc act agc att gat att gct        336
Asn Arg Arg Gly Val Thr Lys Leu Asn Leu Thr Ser Ile Asp Ile Ala           
            100                 105                 110                   

gca ttt agc gaa ttg aac act atg tgc tat tct cct cat tca ctg aaa        384
Ala Phe Ser Glu Leu Asn Thr Met Cys Tyr Ser Pro His Ser Leu Lys           
        115                 120                 125                       

aac aat atc tac tgt ggt ggt gac aac aca aat tgg gga att gcg tcc        432
Asn Asn Ile Tyr Cys Gly Gly Asp Asn Thr Asn Trp Gly Ile Ala Ser           
    130                 135                 140                           

att gac ttg aac aga ggt tgc tta gat tcc ctc ttg aat tac tca tct        480
Ile Asp Leu Asn Arg Gly Cys Leu Asp Ser Leu Leu Asn Tyr Ser Ser           
145                 150                 155                 160           

aaa gtg aag tta atg tgc tct aat aat aaa gtt ttg tct atc gga aga        528
Lys Val Lys Leu Met Cys Ser Asn Asn Lys Val Leu Ser Ile Gly Arg           
                165                 170                 175               

caa aca ggg act gtg gat ttg cta gat cca aca tcg aat cgt act atc        576
Gln Thr Gly Thr Val Asp Leu Leu Asp Pro Thr Ser Asn Arg Thr Ile           
            180                 185                 190                   

aaa tca ttt aat gca cac tct gca tcc ata tcc gct atg gat tta cgg        624
Lys Ser Phe Asn Ala His Ser Ala Ser Ile Ser Ala Met Asp Leu Arg           
        195                 200                 205                       

gat aac acc ttg gtt aca gta ggg aag tcc aaa aga ttt tat aac tta        672
Asp Asn Thr Leu Val Thr Val Gly Lys Ser Lys Arg Phe Tyr Asn Leu           
    210                 215                 220                           

tac gct gac cca ttt gtg aat gtt tac gac ttg aga aca atg cgt caa        720
Tyr Ala Asp Pro Phe Val Asn Val Tyr Asp Leu Arg Thr Met Arg Gln           
225                 230                 235                 240           

ctc cct cct gtt tcc ttt tct aaa gga aca act atg gga tct gga ggc        768
Leu Pro Pro Val Ser Phe Ser Lys Gly Thr Thr Met Gly Ser Gly Gly           
                245                 250                 255               

gca gat ttt gtt caa tta cat cct ttg ctt cct act gtt atg atc gtc        816
Ala Asp Phe Val Gln Leu His Pro Leu Leu Pro Thr Val Met Ile Val           
            260                 265                 270                   

gcc tca agt tct ggt tcg ttt gat ttc atc gac ctt tcc aat cca act        864
Ala Ser Ser Ser Gly Ser Phe Asp Phe Ile Asp Leu Ser Asn Pro Thr           
        275                 280                 285                       

tta aga aca caa tat gtt cat cct tgc cag tcg att aaa aag tta tgt        912
Leu Arg Thr Gln Tyr Val His Pro Cys Gln Ser Ile Lys Lys Leu Cys           
    290                 295                 300                           

ttg tcc ccc aat ggt gac gta ttg ggt ata cta gaa gct gat aat cac        960
Leu Ser Pro Asn Gly Asp Val Leu Gly Ile Leu Glu Ala Asp Asn His           
305                 310                 315                 320           

cta gat aca tgg aga aga tca tca aac aac atg gga atg ttt acc aat       1008
Leu Asp Thr Trp Arg Arg Ser Ser Asn Asn Met Gly Met Phe Thr Asn           
                325                 330                 335               

acc cct gaa atg cta gca tat cct gat tat ttt aat gac att acc tct       1056
Thr Pro Glu Met Leu Ala Tyr Pro Asp Tyr Phe Asn Asp Ile Thr Ser           
            340                 345                 350                   

gac ggc cca ata tct gtc gac gat gaa aca tat cca ttg agt tct gtg       1104
Asp Gly Pro Ile Ser Val Asp Asp Glu Thr Tyr Pro Leu Ser Ser Val           
        355                 360                 365                       

ggg atg ccg tac tat ctt gat aaa ctt ttg tct gca tgg ccc cct gta       1152
Gly Met Pro Tyr Tyr Leu Asp Lys Leu Leu Ser Ala Trp Pro Pro Val           
    370                 375                 380                           

gtg ttt aaa agt gaa ggt acc ata ccg caa tta aca ggt aag tca ccc       1200
Val Phe Lys Ser Glu Gly Thr Ile Pro Gln Leu Thr Gly Lys Ser Pro           
385                 390                 395                 400           

tta cca tcg agc ggc aaa tta aaa agt aac ctt gct gtg atc tcg agc       1248
Leu Pro Ser Ser Gly Lys Leu Lys Ser Asn Leu Ala Val Ile Ser Ser           
                405                 410                 415               

caa aat gag aag ttg agc aca caa gaa ttt cct ttg tta aga tat gat       1296
Gln Asn Glu Lys Leu Ser Thr Gln Glu Phe Pro Leu Leu Arg Tyr Asp           
            420                 425                 430                   

cgc acc aaa tac ggt atg aga aat gct ata cca gat tac gtt tgt cta       1344
Arg Thr Lys Tyr Gly Met Arg Asn Ala Ile Pro Asp Tyr Val Cys Leu           
        435                 440                 445                       

aga gat ata agg aaa cag ata aca agc ggt tta gaa acc agc gat ata       1392
Arg Asp Ile Arg Lys Gln Ile Thr Ser Gly Leu Glu Thr Ser Asp Ile           
    450                 455                 460                           

cag aca tat acc tca atc aac aag tac gaa gta ccc cct gca tac agt       1440
Gln Thr Tyr Thr Ser Ile Asn Lys Tyr Glu Val Pro Pro Ala Tyr Ser           
465                 470                 475                 480           

aga ctt cca ctg aca tca ggt aga ttt ggt act gat aat ttt gat ttt       1488
Arg Leu Pro Leu Thr Ser Gly Arg Phe Gly Thr Asp Asn Phe Asp Phe           
                485                 490                 495               

acg ccc ttt aat aac act gag tat tca gga ttg gat cca gat gtt gat       1536
Thr Pro Phe Asn Asn Thr Glu Tyr Ser Gly Leu Asp Pro Asp Val Asp           
            500                 505                 510                   

aat cac tac aca aat gct atc ata caa ttg tat cgc ttt att cca gaa       1584
Asn His Tyr Thr Asn Ala Ile Ile Gln Leu Tyr Arg Phe Ile Pro Glu           
        515                 520                 525                       

atg ttt aat ttc gtt gtt ggg tgt ttg aaa gac gag aat ttt gaa aca       1632
Met Phe Asn Phe Val Val Gly Cys Leu Lys Asp Glu Asn Phe Glu Thr           
    530                 535                 540                           

acg ttg cta act gat cta ggc tac ctc ttt gac atg atg gaa aga tca       1680
Thr Leu Leu Thr Asp Leu Gly Tyr Leu Phe Asp Met Met Glu Arg Ser           
545                 550                 555                 560           

cat gga aaa ata tgt agt tct tcc aat ttt cag gcg tca ttg aaa tcc       1728
His Gly Lys Ile Cys Ser Ser Ser Asn Phe Gln Ala Ser Leu Lys Ser           
                565                 570                 575               

tta act gat aaa aga caa tta gaa aac ggt gaa cca caa gaa cat tta       1776
Leu Thr Asp Lys Arg Gln Leu Glu Asn Gly Glu Pro Gln Glu His Leu           
            580                 585                 590                   

gaa gag tat tta gaa tcg ctg tgc ata agg gaa agt atc gag gat ttt       1824
Glu Glu Tyr Leu Glu Ser Leu Cys Ile Arg Glu Ser Ile Glu Asp Phe           
        595                 600                 605                       

aat tct tct gaa agt att aaa cgc aat atg cct caa aaa ttt aac aga       1872
Asn Ser Ser Glu Ser Ile Lys Arg Asn Met Pro Gln Lys Phe Asn Arg           
    610                 615                 620                           

ttc ctg ctc tcg caa ctt att aaa gag gaa gcg cag aca gtc aac cat       1920
Phe Leu Leu Ser Gln Leu Ile Lys Glu Glu Ala Gln Thr Val Asn His           
625                 630                 635                 640           

aat atc acc cta aat caa tgc ttt ggt ttg gaa acg gaa ata cga aca       1968
Asn Ile Thr Leu Asn Gln Cys Phe Gly Leu Glu Thr Glu Ile Arg Thr           
                645                 650                 655               

gag tgt agc tgt gat cac tac gac act acc gtc aaa ctt cta ccc tcc       2016
Glu Cys Ser Cys Asp His Tyr Asp Thr Thr Val Lys Leu Leu Pro Ser           
            660                 665                 670                   

tta tca ata tca gga atc aac aaa acc gta atc aaa caa ttg aac aag       2064
Leu Ser Ile Ser Gly Ile Asn Lys Thr Val Ile Lys Gln Leu Asn Lys           
        675                 680                 685                       

aaa agc aat gga cag aat att ttg cct tat att gaa tat gcc atg aaa       2112
Lys Ser Asn Gly Gln Asn Ile Leu Pro Tyr Ile Glu Tyr Ala Met Lys           
    690                 695                 700                           

aat gta acc caa aag aac agt att tgc cca acc tgc ggc aaa acc gaa       2160
Asn Val Thr Gln Lys Asn Ser Ile Cys Pro Thr Cys Gly Lys Thr Glu           
705                 710                 715                 720           

act atc acc cag gag tgt act gtc aag aat tta cct tca gtg ttg tca       2208
Thr Ile Thr Gln Glu Cys Thr Val Lys Asn Leu Pro Ser Val Leu Ser           
                725                 730                 735               

tta gaa tta tca cta tta gat acc gaa ttt tcc aat ata agg tcg tcg       2256
Leu Glu Leu Ser Leu Leu Asp Thr Glu Phe Ser Asn Ile Arg Ser Ser           
            740                 745                 750                   

aaa aac tgg tta act agt gaa ttt tat gga agc atc att aaa aac aag       2304
Lys Asn Trp Leu Thr Ser Glu Phe Tyr Gly Ser Ile Ile Lys Asn Lys           
        755                 760                 765                       

gca gtt cta aga tcg acg gcg tcc gaa ttg aag ggc aca agc cac ata       2352
Ala Val Leu Arg Ser Thr Ala Ser Glu Leu Lys Gly Thr Ser His Ile           
    770                 775                 780                           

ttt aaa tac gaa ttg aat ggt tac gtg gct aaa atc act gat aac aat       2400
Phe Lys Tyr Glu Leu Asn Gly Tyr Val Ala Lys Ile Thr Asp Asn Asn           
785                 790                 795                 800           

aac gag acg cgt cta gta aca tat gtc aaa aaa tat aat cca aaa gag       2448
Asn Glu Thr Arg Leu Val Thr Tyr Val Lys Lys Tyr Asn Pro Lys Glu           
                805                 810                 815               

aat tgc ttc aag tgg ctc atg ttt aat gat tat ttg gtt gtt gag ata       2496
Asn Cys Phe Lys Trp Leu Met Phe Asn Asp Tyr Leu Val Val Glu Ile           
            820                 825                 830                   

aca gag gaa gag gcg ctt aaa atg aca tac cct tgg aaa aca cca gaa       2544
Thr Glu Glu Glu Ala Leu Lys Met Thr Tyr Pro Trp Lys Thr Pro Glu           
        835                 840                 845                       

att atc ata tat tgt gat gcg gaa gaa tta cga aaa cct ttc ttt tct       2592
Ile Ile Ile Tyr Cys Asp Ala Glu Glu Leu Arg Lys Pro Phe Phe Ser           
    850                 855                 860                           

gtt gat acg tat tcc atc aac tat gac ata ctt ttc cgt gat tat ttc       2640
Val Asp Thr Tyr Ser Ile Asn Tyr Asp Ile Leu Phe Arg Asp Tyr Phe           
865                 870                 875                 880           

gca aac gga ata aga gat act gca aga cgt gaa tat aag tta tta aca       2688
Ala Asn Gly Ile Arg Asp Thr Ala Arg Arg Glu Tyr Lys Leu Leu Thr           
                885                 890                 895               

cat gat gag gca cct aaa tct gga acc ttg gtt gcc att gat gcc gaa       2736
His Asp Glu Ala Pro Lys Ser Gly Thr Leu Val Ala Ile Asp Ala Glu           
            900                 905                 910                   

ttt gtc tca tta caa agt gaa cta tgt gaa atc gat cat caa gga atc       2784
Phe Val Ser Leu Gln Ser Glu Leu Cys Glu Ile Asp His Gln Gly Ile           
        915                 920                 925                       

aga agt att att cga cct aaa aga act gct ttg gcc aga ata tcc att       2832
Arg Ser Ile Ile Arg Pro Lys Arg Thr Ala Leu Ala Arg Ile Ser Ile           
    930                 935                 940                           

att aga ggc gaa gaa gga gaa ctg tat gga gta cca ttt gtc gat gat       2880
Ile Arg Gly Glu Glu Gly Glu Leu Tyr Gly Val Pro Phe Val Asp Asp           
945                 950                 955                 960           

tat gtg gta aac acg aac cac ata gaa gac tat ttg aca aga tat agt       2928
Tyr Val Val Asn Thr Asn His Ile Glu Asp Tyr Leu Thr Arg Tyr Ser           
                965                 970                 975               

ggg att ctt cct ggt gac ttg gac cct gaa aag agt acc aaa agg ctt       2976
Gly Ile Leu Pro Gly Asp Leu Asp Pro Glu Lys Ser Thr Lys Arg Leu           
            980                 985                 990                   

gtg aga aga aac gtt gta tat cga  aaa gtc tgg ctt tta  atg cag ctc     3024
Val Arg Arg Asn Val Val Tyr Arg  Lys Val Trp Leu Leu  Met Gln Leu         
        995                 1000                 1005                     

gga tgc  gta ttt gtt ggt cat  ggt ttg aat aat gac  ttc aaa cac        3069
Gly Cys  Val Phe Val Gly His  Gly Leu Asn Asn Asp  Phe Lys His            
    1010                 1015                 1020                        

att aat  att aat gtc cca aga  aac caa att cgc gac  act gcc ata        3114
Ile Asn  Ile Asn Val Pro Arg  Asn Gln Ile Arg Asp  Thr Ala Ile            
    1025                 1030                 1035                        

tat ttt  cta caa gga aag aga  tat ctt tca ttg cgt  tat ctg gca        3159
Tyr Phe  Leu Gln Gly Lys Arg  Tyr Leu Ser Leu Arg  Tyr Leu Ala            
    1040                 1045                 1050                        

tat gtg  ttg tta gga atg aat  atc caa gag gga aat  cac gat tca        3204
Tyr Val  Leu Leu Gly Met Asn  Ile Gln Glu Gly Asn  His Asp Ser            
    1055                 1060                 1065                        

att gaa  gat gcc cat act gcc  ttg att ctt tac aaa  aaa tat ctc        3249
Ile Glu  Asp Ala His Thr Ala  Leu Ile Leu Tyr Lys  Lys Tyr Leu            
    1070                 1075                 1080                        

cac ctg  aaa gaa aaa gct atc  ttt gag aaa gta ctg  aac agc gtg        3294
His Leu  Lys Glu Lys Ala Ile  Phe Glu Lys Val Leu  Asn Ser Val            
    1085                 1090                 1095                        

tac gaa  gaa gga aga gcc cat  aat ttc aaa gtt cca  gaa act tca        3339
Tyr Glu  Glu Gly Arg Ala His  Asn Phe Lys Val Pro  Glu Thr Ser            
    1100                 1105                 1110                        

aag gga  taa                                                          3348
Lys Gly                                                                   
    1115                                                                  


<210>  12
<211>  1115
<212>  PRT
<213>  Saccharomyces cereviciae

<400>  12

Met Asn Asn Trp Gln His Phe Phe Asn Asn Pro Val Asp Leu Ser Glu 
1               5                   10                  15      


His Leu Lys Lys Pro Tyr Phe Arg Phe Asp Asn Arg Asp Lys Glu Ile 
            20                  25                  30          


Thr Ala Ile Ser Phe Asp Glu Lys Ala Asn Leu Ile Trp Ser Gly Asp 
        35                  40                  45              


Ser Tyr Gly Cys Ile Ser Ser Tyr Asp Pro Thr Phe Gln Leu Tyr Thr 
    50                  55                  60                  


Arg Tyr Arg Gly His Ile Gly Gly Asn Ser Val Lys Asp Ile Leu Ser 
65                  70                  75                  80  


His Arg Asp Gly Ile Leu Ser Ile Ser Glu Asp Ser Leu His Phe Ala 
                85                  90                  95      


Asn Arg Arg Gly Val Thr Lys Leu Asn Leu Thr Ser Ile Asp Ile Ala 
            100                 105                 110         


Ala Phe Ser Glu Leu Asn Thr Met Cys Tyr Ser Pro His Ser Leu Lys 
        115                 120                 125             


Asn Asn Ile Tyr Cys Gly Gly Asp Asn Thr Asn Trp Gly Ile Ala Ser 
    130                 135                 140                 


Ile Asp Leu Asn Arg Gly Cys Leu Asp Ser Leu Leu Asn Tyr Ser Ser 
145                 150                 155                 160 


Lys Val Lys Leu Met Cys Ser Asn Asn Lys Val Leu Ser Ile Gly Arg 
                165                 170                 175     


Gln Thr Gly Thr Val Asp Leu Leu Asp Pro Thr Ser Asn Arg Thr Ile 
            180                 185                 190         


Lys Ser Phe Asn Ala His Ser Ala Ser Ile Ser Ala Met Asp Leu Arg 
        195                 200                 205             


Asp Asn Thr Leu Val Thr Val Gly Lys Ser Lys Arg Phe Tyr Asn Leu 
    210                 215                 220                 


Tyr Ala Asp Pro Phe Val Asn Val Tyr Asp Leu Arg Thr Met Arg Gln 
225                 230                 235                 240 


Leu Pro Pro Val Ser Phe Ser Lys Gly Thr Thr Met Gly Ser Gly Gly 
                245                 250                 255     


Ala Asp Phe Val Gln Leu His Pro Leu Leu Pro Thr Val Met Ile Val 
            260                 265                 270         


Ala Ser Ser Ser Gly Ser Phe Asp Phe Ile Asp Leu Ser Asn Pro Thr 
        275                 280                 285             


Leu Arg Thr Gln Tyr Val His Pro Cys Gln Ser Ile Lys Lys Leu Cys 
    290                 295                 300                 


Leu Ser Pro Asn Gly Asp Val Leu Gly Ile Leu Glu Ala Asp Asn His 
305                 310                 315                 320 


Leu Asp Thr Trp Arg Arg Ser Ser Asn Asn Met Gly Met Phe Thr Asn 
                325                 330                 335     


Thr Pro Glu Met Leu Ala Tyr Pro Asp Tyr Phe Asn Asp Ile Thr Ser 
            340                 345                 350         


Asp Gly Pro Ile Ser Val Asp Asp Glu Thr Tyr Pro Leu Ser Ser Val 
        355                 360                 365             


Gly Met Pro Tyr Tyr Leu Asp Lys Leu Leu Ser Ala Trp Pro Pro Val 
    370                 375                 380                 


Val Phe Lys Ser Glu Gly Thr Ile Pro Gln Leu Thr Gly Lys Ser Pro 
385                 390                 395                 400 


Leu Pro Ser Ser Gly Lys Leu Lys Ser Asn Leu Ala Val Ile Ser Ser 
                405                 410                 415     


Gln Asn Glu Lys Leu Ser Thr Gln Glu Phe Pro Leu Leu Arg Tyr Asp 
            420                 425                 430         


Arg Thr Lys Tyr Gly Met Arg Asn Ala Ile Pro Asp Tyr Val Cys Leu 
        435                 440                 445             


Arg Asp Ile Arg Lys Gln Ile Thr Ser Gly Leu Glu Thr Ser Asp Ile 
    450                 455                 460                 


Gln Thr Tyr Thr Ser Ile Asn Lys Tyr Glu Val Pro Pro Ala Tyr Ser 
465                 470                 475                 480 


Arg Leu Pro Leu Thr Ser Gly Arg Phe Gly Thr Asp Asn Phe Asp Phe 
                485                 490                 495     


Thr Pro Phe Asn Asn Thr Glu Tyr Ser Gly Leu Asp Pro Asp Val Asp 
            500                 505                 510         


Asn His Tyr Thr Asn Ala Ile Ile Gln Leu Tyr Arg Phe Ile Pro Glu 
        515                 520                 525             


Met Phe Asn Phe Val Val Gly Cys Leu Lys Asp Glu Asn Phe Glu Thr 
    530                 535                 540                 


Thr Leu Leu Thr Asp Leu Gly Tyr Leu Phe Asp Met Met Glu Arg Ser 
545                 550                 555                 560 


His Gly Lys Ile Cys Ser Ser Ser Asn Phe Gln Ala Ser Leu Lys Ser 
                565                 570                 575     


Leu Thr Asp Lys Arg Gln Leu Glu Asn Gly Glu Pro Gln Glu His Leu 
            580                 585                 590         


Glu Glu Tyr Leu Glu Ser Leu Cys Ile Arg Glu Ser Ile Glu Asp Phe 
        595                 600                 605             


Asn Ser Ser Glu Ser Ile Lys Arg Asn Met Pro Gln Lys Phe Asn Arg 
    610                 615                 620                 


Phe Leu Leu Ser Gln Leu Ile Lys Glu Glu Ala Gln Thr Val Asn His 
625                 630                 635                 640 


Asn Ile Thr Leu Asn Gln Cys Phe Gly Leu Glu Thr Glu Ile Arg Thr 
                645                 650                 655     


Glu Cys Ser Cys Asp His Tyr Asp Thr Thr Val Lys Leu Leu Pro Ser 
            660                 665                 670         


Leu Ser Ile Ser Gly Ile Asn Lys Thr Val Ile Lys Gln Leu Asn Lys 
        675                 680                 685             


Lys Ser Asn Gly Gln Asn Ile Leu Pro Tyr Ile Glu Tyr Ala Met Lys 
    690                 695                 700                 


Asn Val Thr Gln Lys Asn Ser Ile Cys Pro Thr Cys Gly Lys Thr Glu 
705                 710                 715                 720 


Thr Ile Thr Gln Glu Cys Thr Val Lys Asn Leu Pro Ser Val Leu Ser 
                725                 730                 735     


Leu Glu Leu Ser Leu Leu Asp Thr Glu Phe Ser Asn Ile Arg Ser Ser 
            740                 745                 750         


Lys Asn Trp Leu Thr Ser Glu Phe Tyr Gly Ser Ile Ile Lys Asn Lys 
        755                 760                 765             


Ala Val Leu Arg Ser Thr Ala Ser Glu Leu Lys Gly Thr Ser His Ile 
    770                 775                 780                 


Phe Lys Tyr Glu Leu Asn Gly Tyr Val Ala Lys Ile Thr Asp Asn Asn 
785                 790                 795                 800 


Asn Glu Thr Arg Leu Val Thr Tyr Val Lys Lys Tyr Asn Pro Lys Glu 
                805                 810                 815     


Asn Cys Phe Lys Trp Leu Met Phe Asn Asp Tyr Leu Val Val Glu Ile 
            820                 825                 830         


Thr Glu Glu Glu Ala Leu Lys Met Thr Tyr Pro Trp Lys Thr Pro Glu 
        835                 840                 845             


Ile Ile Ile Tyr Cys Asp Ala Glu Glu Leu Arg Lys Pro Phe Phe Ser 
    850                 855                 860                 


Val Asp Thr Tyr Ser Ile Asn Tyr Asp Ile Leu Phe Arg Asp Tyr Phe 
865                 870                 875                 880 


Ala Asn Gly Ile Arg Asp Thr Ala Arg Arg Glu Tyr Lys Leu Leu Thr 
                885                 890                 895     


His Asp Glu Ala Pro Lys Ser Gly Thr Leu Val Ala Ile Asp Ala Glu 
            900                 905                 910         


Phe Val Ser Leu Gln Ser Glu Leu Cys Glu Ile Asp His Gln Gly Ile 
        915                 920                 925             


Arg Ser Ile Ile Arg Pro Lys Arg Thr Ala Leu Ala Arg Ile Ser Ile 
    930                 935                 940                 


Ile Arg Gly Glu Glu Gly Glu Leu Tyr Gly Val Pro Phe Val Asp Asp 
945                 950                 955                 960 


Tyr Val Val Asn Thr Asn His Ile Glu Asp Tyr Leu Thr Arg Tyr Ser 
                965                 970                 975     


Gly Ile Leu Pro Gly Asp Leu Asp Pro Glu Lys Ser Thr Lys Arg Leu 
            980                 985                 990         


Val Arg Arg Asn Val Val Tyr Arg  Lys Val Trp Leu Leu  Met Gln Leu 
        995                 1000                 1005             


Gly Cys  Val Phe Val Gly His  Gly Leu Asn Asn Asp  Phe Lys His 
    1010                 1015                 1020             


Ile Asn  Ile Asn Val Pro Arg  Asn Gln Ile Arg Asp  Thr Ala Ile 
    1025                 1030                 1035             


Tyr Phe  Leu Gln Gly Lys Arg  Tyr Leu Ser Leu Arg  Tyr Leu Ala 
    1040                 1045                 1050             


Tyr Val  Leu Leu Gly Met Asn  Ile Gln Glu Gly Asn  His Asp Ser 
    1055                 1060                 1065             


Ile Glu  Asp Ala His Thr Ala  Leu Ile Leu Tyr Lys  Lys Tyr Leu 
    1070                 1075                 1080             


His Leu  Lys Glu Lys Ala Ile  Phe Glu Lys Val Leu  Asn Ser Val 
    1085                 1090                 1095             


Tyr Glu  Glu Gly Arg Ala His  Asn Phe Lys Val Pro  Glu Thr Ser 
    1100                 1105                 1110             


Lys Gly  
    1115 


<210>  13
<211>  2040
<212>  DNA
<213>  Saccharomyces cereviciae


<220>
<221>  CDS
<222>  (1)..(2040)
<223>  Pan3

<400>  13
atg gac aaa atc aat cct gat tgg gcg aag gat att ccg tgc aga aat         48
Met Asp Lys Ile Asn Pro Asp Trp Ala Lys Asp Ile Pro Cys Arg Asn           
1               5                   10                  15                

atc act att tat ggc tac tgc aaa aag gag aaa gaa ggt tgc cct ttc         96
Ile Thr Ile Tyr Gly Tyr Cys Lys Lys Glu Lys Glu Gly Cys Pro Phe           
            20                  25                  30                    

aaa cac agc gat aac act acc gct act acc ata aat gac gtt cct cct        144
Lys His Ser Asp Asn Thr Thr Ala Thr Thr Ile Asn Asp Val Pro Pro           
        35                  40                  45                        

cca ata gat gtg ggt gag gct aca act ccg acc atg aca tca gtt cct        192
Pro Ile Asp Val Gly Glu Ala Thr Thr Pro Thr Met Thr Ser Val Pro           
    50                  55                  60                            

aag ttc aat gct aaa gta tcc gca agt ttc act ccg atg aca gtc ggt        240
Lys Phe Asn Ala Lys Val Ser Ala Ser Phe Thr Pro Met Thr Val Gly           
65                  70                  75                  80            

agt gac tcc tta acc act gtg acg aat acc acc tcc gct gct aca aat        288
Ser Asp Ser Leu Thr Thr Val Thr Asn Thr Thr Ser Ala Ala Thr Asn           
                85                  90                  95                

gct act ggc aat atc gcc atg gca gct acc tct gct act gct tct aca        336
Ala Thr Gly Asn Ile Ala Met Ala Ala Thr Ser Ala Thr Ala Ser Thr           
            100                 105                 110                   

gtt aat ccg atg att aat ccg ata gtt aat agc tcg tta gtg aat aac        384
Val Asn Pro Met Ile Asn Pro Ile Val Asn Ser Ser Leu Val Asn Asn           
        115                 120                 125                       

aat aac aat aat agt aat ata agc ata tca ata cca act acc gct tcg        432
Asn Asn Asn Asn Ser Asn Ile Ser Ile Ser Ile Pro Thr Thr Ala Ser           
    130                 135                 140                           

agt tcc aat tac gac ccc ttc aat gcc ccc att ttc act ccg tct tca        480
Ser Ser Asn Tyr Asp Pro Phe Asn Ala Pro Ile Phe Thr Pro Ser Ser           
145                 150                 155                 160           

acc tcc tca att cac act aat gca aat gca cat tct ttt cca ttt ccc        528
Thr Ser Ser Ile His Thr Asn Ala Asn Ala His Ser Phe Pro Phe Pro           
                165                 170                 175               

tcc att gca aat tct ggt ggc ata aat ata aac gcc act gat gat aat        576
Ser Ile Ala Asn Ser Gly Gly Ile Asn Ile Asn Ala Thr Asp Asp Asn           
            180                 185                 190                   

agt aac aat atg agt atg gct aat aat gtg cca cct cct atg caa ccg        624
Ser Asn Asn Met Ser Met Ala Asn Asn Val Pro Pro Pro Met Gln Pro           
        195                 200                 205                       

cca ccc ata gag agt agt aat ctt aag tac cca cgt att tat ccg cct        672
Pro Pro Ile Glu Ser Ser Asn Leu Lys Tyr Pro Arg Ile Tyr Pro Pro           
    210                 215                 220                           

cct cac agt ctt cta cag tat cac cta tat gca cct gaa cag cca tca        720
Pro His Ser Leu Leu Gln Tyr His Leu Tyr Ala Pro Glu Gln Pro Ser           
225                 230                 235                 240           

tca ttg aaa tca tta tta aag cct aat gaa agg tct gca gat cag ctt        768
Ser Leu Lys Ser Leu Leu Lys Pro Asn Glu Arg Ser Ala Asp Gln Leu           
                245                 250                 255               

ttc att cca aac aat att aga gaa gat tta acc aag aaa aac tta tcg        816
Phe Ile Pro Asn Asn Ile Arg Glu Asp Leu Thr Lys Lys Asn Leu Ser           
            260                 265                 270                   

att ttg cag gtt ttc ccc tct tca ggt aaa gtt ata cca agt att gta        864
Ile Leu Gln Val Phe Pro Ser Ser Gly Lys Val Ile Pro Ser Ile Val           
        275                 280                 285                       

caa gat tat ttt aat ttg gtt cca ttg aac ttc aat aat aac gat ttt        912
Gln Asp Tyr Phe Asn Leu Val Pro Leu Asn Phe Asn Asn Asn Asp Phe           
    290                 295                 300                           

tta aat aaa act acg ctc ttc aaa gtt ttt tcc aat tat gac ggt aaa        960
Leu Asn Lys Thr Thr Leu Phe Lys Val Phe Ser Asn Tyr Asp Gly Lys           
305                 310                 315                 320           

gcc tac gtt ttg aag agg ctt cct aac atc gat aag tca atg aat cca       1008
Ala Tyr Val Leu Lys Arg Leu Pro Asn Ile Asp Lys Ser Met Asn Pro           
                325                 330                 335               

aac aaa ata tcc aaa ata tat cag ata tgg tca aaa att aat tgt aca       1056
Asn Lys Ile Ser Lys Ile Tyr Gln Ile Trp Ser Lys Ile Asn Cys Thr           
            340                 345                 350                   

aat ttg ata aag ttt agg gac att ttt caa act act aaa ttt ggt gat       1104
Asn Leu Ile Lys Phe Arg Asp Ile Phe Gln Thr Thr Lys Phe Gly Asp           
        355                 360                 365                       

ctg tct att tgt ttg gtc ttt gac tac tac cca aac tcg cta tct ttg       1152
Leu Ser Ile Cys Leu Val Phe Asp Tyr Tyr Pro Asn Ser Leu Ser Leu           
    370                 375                 380                           

tat gat tac cac ttt gtt aat ttc cct aag ttt cca ata acg aat aat       1200
Tyr Asp Tyr His Phe Val Asn Phe Pro Lys Phe Pro Ile Thr Asn Asn           
385                 390                 395                 400           

tat tta tgg ata tat tta gtt caa ctc acc aat gta ata aac tct atc       1248
Tyr Leu Trp Ile Tyr Leu Val Gln Leu Thr Asn Val Ile Asn Ser Ile           
                405                 410                 415               

cat tca caa aac ttg agt att ggc aat aca tta aac tgg aga aaa gtt       1296
His Ser Gln Asn Leu Ser Ile Gly Asn Thr Leu Asn Trp Arg Lys Val           
            420                 425                 430                   

ttg att act ggg gac cca ggg aga atc aag tta tca cac tgc aat ttt       1344
Leu Ile Thr Gly Asp Pro Gly Arg Ile Lys Leu Ser His Cys Asn Phe           
        435                 440                 445                       

atg gac ctt ttg ttc aat gat gat act gat acc gta gta tct tcc ggc       1392
Met Asp Leu Leu Phe Asn Asp Asp Thr Asp Thr Val Val Ser Ser Gly           
    450                 455                 460                           

gga agt acc ata gag gga caa caa cag cta gac tac aaa tat tta gga       1440
Gly Ser Thr Ile Glu Gly Gln Gln Gln Leu Asp Tyr Lys Tyr Leu Gly           
465                 470                 475                 480           

gag cta tta ttt aac cta tcc att aat att gaa aac tct aat aac aac       1488
Glu Leu Leu Phe Asn Leu Ser Ile Asn Ile Glu Asn Ser Asn Asn Asn           
                485                 490                 495               

act gcc cct aaa gaa tat cga ttg gag gaa ata acc cct caa tca att       1536
Thr Ala Pro Lys Glu Tyr Arg Leu Glu Glu Ile Thr Pro Gln Ser Ile           
            500                 505                 510                   

gat gac atg aga cag atc gat gat aag ttc aag gat gta ctc aag tat       1584
Asp Asp Met Arg Gln Ile Asp Asp Lys Phe Lys Asp Val Leu Lys Tyr           
        515                 520                 525                       

ctg ata tca gac aac ggc gat tcc aaa aag agc att cat gat ctt act       1632
Leu Ile Ser Asp Asn Gly Asp Ser Lys Lys Ser Ile His Asp Leu Thr           
    530                 535                 540                           

agt cac ttt tat gat aag atg ttc atg gtc ctg gaa tcg tca caa acc       1680
Ser His Phe Tyr Asp Lys Met Phe Met Val Leu Glu Ser Ser Gln Thr           
545                 550                 555                 560           

tat aca gaa tac atg gag tct gtc tta tca aga gaa cta gaa aat ggc       1728
Tyr Thr Glu Tyr Met Glu Ser Val Leu Ser Arg Glu Leu Glu Asn Gly           
                565                 570                 575               

aga tta ttt agg ctg gtc aac aag cta aat tgc att ttt ggt aga atc       1776
Arg Leu Phe Arg Leu Val Asn Lys Leu Asn Cys Ile Phe Gly Arg Ile           
            580                 585                 590                   

gaa tca aga ata gac ata aat tgg tcc gaa tct ggg act aaa ttc ccc       1824
Glu Ser Arg Ile Asp Ile Asn Trp Ser Glu Ser Gly Thr Lys Phe Pro           
        595                 600                 605                       

att ata cta ttt tat gac tac gta ttc cat caa gtg gat tcg aat ggg       1872
Ile Ile Leu Phe Tyr Asp Tyr Val Phe His Gln Val Asp Ser Asn Gly           
    610                 615                 620                           

aaa cca ata atg gat tta act cat gtc cta aga tgt ttg aac aaa tta       1920
Lys Pro Ile Met Asp Leu Thr His Val Leu Arg Cys Leu Asn Lys Leu           
625                 630                 635                 640           

gac gct ggt att caa gaa aag tta atg ttg gta acg ccc gat gag tta       1968
Asp Ala Gly Ile Gln Glu Lys Leu Met Leu Val Thr Pro Asp Glu Leu           
                645                 650                 655               

aac tgt att att ata tcc tat aag gag ttg aag gac ttg ata gaa tcc       2016
Asn Cys Ile Ile Ile Ser Tyr Lys Glu Leu Lys Asp Leu Ile Glu Ser           
            660                 665                 670                   

acc ttt cga tcc atc acc caa taa                                       2040
Thr Phe Arg Ser Ile Thr Gln                                               
        675                                                               


<210>  14
<211>  679
<212>  PRT
<213>  Saccharomyces cereviciae

<400>  14

Met Asp Lys Ile Asn Pro Asp Trp Ala Lys Asp Ile Pro Cys Arg Asn 
1               5                   10                  15      


Ile Thr Ile Tyr Gly Tyr Cys Lys Lys Glu Lys Glu Gly Cys Pro Phe 
            20                  25                  30          


Lys His Ser Asp Asn Thr Thr Ala Thr Thr Ile Asn Asp Val Pro Pro 
        35                  40                  45              


Pro Ile Asp Val Gly Glu Ala Thr Thr Pro Thr Met Thr Ser Val Pro 
    50                  55                  60                  


Lys Phe Asn Ala Lys Val Ser Ala Ser Phe Thr Pro Met Thr Val Gly 
65                  70                  75                  80  


Ser Asp Ser Leu Thr Thr Val Thr Asn Thr Thr Ser Ala Ala Thr Asn 
                85                  90                  95      


Ala Thr Gly Asn Ile Ala Met Ala Ala Thr Ser Ala Thr Ala Ser Thr 
            100                 105                 110         


Val Asn Pro Met Ile Asn Pro Ile Val Asn Ser Ser Leu Val Asn Asn 
        115                 120                 125             


Asn Asn Asn Asn Ser Asn Ile Ser Ile Ser Ile Pro Thr Thr Ala Ser 
    130                 135                 140                 


Ser Ser Asn Tyr Asp Pro Phe Asn Ala Pro Ile Phe Thr Pro Ser Ser 
145                 150                 155                 160 


Thr Ser Ser Ile His Thr Asn Ala Asn Ala His Ser Phe Pro Phe Pro 
                165                 170                 175     


Ser Ile Ala Asn Ser Gly Gly Ile Asn Ile Asn Ala Thr Asp Asp Asn 
            180                 185                 190         


Ser Asn Asn Met Ser Met Ala Asn Asn Val Pro Pro Pro Met Gln Pro 
        195                 200                 205             


Pro Pro Ile Glu Ser Ser Asn Leu Lys Tyr Pro Arg Ile Tyr Pro Pro 
    210                 215                 220                 


Pro His Ser Leu Leu Gln Tyr His Leu Tyr Ala Pro Glu Gln Pro Ser 
225                 230                 235                 240 


Ser Leu Lys Ser Leu Leu Lys Pro Asn Glu Arg Ser Ala Asp Gln Leu 
                245                 250                 255     


Phe Ile Pro Asn Asn Ile Arg Glu Asp Leu Thr Lys Lys Asn Leu Ser 
            260                 265                 270         


Ile Leu Gln Val Phe Pro Ser Ser Gly Lys Val Ile Pro Ser Ile Val 
        275                 280                 285             


Gln Asp Tyr Phe Asn Leu Val Pro Leu Asn Phe Asn Asn Asn Asp Phe 
    290                 295                 300                 


Leu Asn Lys Thr Thr Leu Phe Lys Val Phe Ser Asn Tyr Asp Gly Lys 
305                 310                 315                 320 


Ala Tyr Val Leu Lys Arg Leu Pro Asn Ile Asp Lys Ser Met Asn Pro 
                325                 330                 335     


Asn Lys Ile Ser Lys Ile Tyr Gln Ile Trp Ser Lys Ile Asn Cys Thr 
            340                 345                 350         


Asn Leu Ile Lys Phe Arg Asp Ile Phe Gln Thr Thr Lys Phe Gly Asp 
        355                 360                 365             


Leu Ser Ile Cys Leu Val Phe Asp Tyr Tyr Pro Asn Ser Leu Ser Leu 
    370                 375                 380                 


Tyr Asp Tyr His Phe Val Asn Phe Pro Lys Phe Pro Ile Thr Asn Asn 
385                 390                 395                 400 


Tyr Leu Trp Ile Tyr Leu Val Gln Leu Thr Asn Val Ile Asn Ser Ile 
                405                 410                 415     


His Ser Gln Asn Leu Ser Ile Gly Asn Thr Leu Asn Trp Arg Lys Val 
            420                 425                 430         


Leu Ile Thr Gly Asp Pro Gly Arg Ile Lys Leu Ser His Cys Asn Phe 
        435                 440                 445             


Met Asp Leu Leu Phe Asn Asp Asp Thr Asp Thr Val Val Ser Ser Gly 
    450                 455                 460                 


Gly Ser Thr Ile Glu Gly Gln Gln Gln Leu Asp Tyr Lys Tyr Leu Gly 
465                 470                 475                 480 


Glu Leu Leu Phe Asn Leu Ser Ile Asn Ile Glu Asn Ser Asn Asn Asn 
                485                 490                 495     


Thr Ala Pro Lys Glu Tyr Arg Leu Glu Glu Ile Thr Pro Gln Ser Ile 
            500                 505                 510         


Asp Asp Met Arg Gln Ile Asp Asp Lys Phe Lys Asp Val Leu Lys Tyr 
        515                 520                 525             


Leu Ile Ser Asp Asn Gly Asp Ser Lys Lys Ser Ile His Asp Leu Thr 
    530                 535                 540                 


Ser His Phe Tyr Asp Lys Met Phe Met Val Leu Glu Ser Ser Gln Thr 
545                 550                 555                 560 


Tyr Thr Glu Tyr Met Glu Ser Val Leu Ser Arg Glu Leu Glu Asn Gly 
                565                 570                 575     


Arg Leu Phe Arg Leu Val Asn Lys Leu Asn Cys Ile Phe Gly Arg Ile 
            580                 585                 590         


Glu Ser Arg Ile Asp Ile Asn Trp Ser Glu Ser Gly Thr Lys Phe Pro 
        595                 600                 605             


Ile Ile Leu Phe Tyr Asp Tyr Val Phe His Gln Val Asp Ser Asn Gly 
    610                 615                 620                 


Lys Pro Ile Met Asp Leu Thr His Val Leu Arg Cys Leu Asn Lys Leu 
625                 630                 635                 640 


Asp Ala Gly Ile Gln Glu Lys Leu Met Leu Val Thr Pro Asp Glu Leu 
                645                 650                 655     


Asn Cys Ile Ile Ile Ser Tyr Lys Glu Leu Lys Asp Leu Ile Glu Ser 
            660                 665                 670         


Thr Phe Arg Ser Ile Thr Gln 
        675                 


<210>  15
<211>  666
<212>  DNA
<213>  Saccharomyces cereviciae


<220>
<221>  CDS
<222>  (1)..(666)
<223>  Rpb4

<400>  15
atg aat gtt tct aca tca acc ttt caa aca aga cgg aga aga ttg aag         48
Met Asn Val Ser Thr Ser Thr Phe Gln Thr Arg Arg Arg Arg Leu Lys           
1               5                   10                  15                

aaa gtg gag gaa gaa gaa aat gca gct act cta caa ctg ggc cag gaa         96
Lys Val Glu Glu Glu Glu Asn Ala Ala Thr Leu Gln Leu Gly Gln Glu           
            20                  25                  30                    

ttc cag ctg aaa cag ata aat cat cag ggt gaa gag gag gaa ttg att        144
Phe Gln Leu Lys Gln Ile Asn His Gln Gly Glu Glu Glu Glu Leu Ile           
        35                  40                  45                        

gcc ttg aac cta agt gaa gcc agg tta gta atc aaa gaa gct ctt gta        192
Ala Leu Asn Leu Ser Glu Ala Arg Leu Val Ile Lys Glu Ala Leu Val           
    50                  55                  60                            

gaa cgt agg aga gca ttt aaa aga tcg caa aaa aaa cac aag aag aag        240
Glu Arg Arg Arg Ala Phe Lys Arg Ser Gln Lys Lys His Lys Lys Lys           
65                  70                  75                  80            

cat ttg aag cac gaa aac gcc aat gat gaa act acg gca gta gag gat        288
His Leu Lys His Glu Asn Ala Asn Asp Glu Thr Thr Ala Val Glu Asp           
                85                  90                  95                

gaa gat gat gat ctg gat gaa gat gac gtc aac gct gat gat gat gat        336
Glu Asp Asp Asp Leu Asp Glu Asp Asp Val Asn Ala Asp Asp Asp Asp           
            100                 105                 110                   

ttt atg cat tct gaa act agg gag aag gag ttg gag tct atc gac gtt        384
Phe Met His Ser Glu Thr Arg Glu Lys Glu Leu Glu Ser Ile Asp Val           
        115                 120                 125                       

ctg tta gaa cag aca acg gga gga aat aat aaa gat ttg aaa aat acc        432
Leu Leu Glu Gln Thr Thr Gly Gly Asn Asn Lys Asp Leu Lys Asn Thr           
    130                 135                 140                           

atg cag tat tta aca aat ttc tcc cga ttt aga gac caa gaa acc gtc        480
Met Gln Tyr Leu Thr Asn Phe Ser Arg Phe Arg Asp Gln Glu Thr Val           
145                 150                 155                 160           

ggg gca gtt ata cag ctt ctg aaa agc act ggg tta cat cct ttt gaa        528
Gly Ala Val Ile Gln Leu Leu Lys Ser Thr Gly Leu His Pro Phe Glu           
                165                 170                 175               

gtg gcg caa cta ggt tct ttg gcc tgt gac aca gct gat gaa gca aag        576
Val Ala Gln Leu Gly Ser Leu Ala Cys Asp Thr Ala Asp Glu Ala Lys           
            180                 185                 190                   

act tta att cca agc tta aac aat aaa ata tca gac gat gag ttg gaa        624
Thr Leu Ile Pro Ser Leu Asn Asn Lys Ile Ser Asp Asp Glu Leu Glu           
        195                 200                 205                       

agg ata cta aag gaa ttg tca aac cta gaa aca ctc tat taa                666
Arg Ile Leu Lys Glu Leu Ser Asn Leu Glu Thr Leu Tyr                       
    210                 215                 220                           


<210>  16
<211>  221
<212>  PRT
<213>  Saccharomyces cereviciae

<400>  16

Met Asn Val Ser Thr Ser Thr Phe Gln Thr Arg Arg Arg Arg Leu Lys 
1               5                   10                  15      


Lys Val Glu Glu Glu Glu Asn Ala Ala Thr Leu Gln Leu Gly Gln Glu 
            20                  25                  30          


Phe Gln Leu Lys Gln Ile Asn His Gln Gly Glu Glu Glu Glu Leu Ile 
        35                  40                  45              


Ala Leu Asn Leu Ser Glu Ala Arg Leu Val Ile Lys Glu Ala Leu Val 
    50                  55                  60                  


Glu Arg Arg Arg Ala Phe Lys Arg Ser Gln Lys Lys His Lys Lys Lys 
65                  70                  75                  80  


His Leu Lys His Glu Asn Ala Asn Asp Glu Thr Thr Ala Val Glu Asp 
                85                  90                  95      


Glu Asp Asp Asp Leu Asp Glu Asp Asp Val Asn Ala Asp Asp Asp Asp 
            100                 105                 110         


Phe Met His Ser Glu Thr Arg Glu Lys Glu Leu Glu Ser Ile Asp Val 
        115                 120                 125             


Leu Leu Glu Gln Thr Thr Gly Gly Asn Asn Lys Asp Leu Lys Asn Thr 
    130                 135                 140                 


Met Gln Tyr Leu Thr Asn Phe Ser Arg Phe Arg Asp Gln Glu Thr Val 
145                 150                 155                 160 


Gly Ala Val Ile Gln Leu Leu Lys Ser Thr Gly Leu His Pro Phe Glu 
                165                 170                 175     


Val Ala Gln Leu Gly Ser Leu Ala Cys Asp Thr Ala Asp Glu Ala Lys 
            180                 185                 190         


Thr Leu Ile Pro Ser Leu Asn Asn Lys Ile Ser Asp Asp Glu Leu Glu 
        195                 200                 205             


Arg Ile Leu Lys Glu Leu Ser Asn Leu Glu Thr Leu Tyr 
    210                 215                 220     


<210>  17
<211>  516
<212>  DNA
<213>  Saccharomyces cereviciae


<220>
<221>  CDS
<222>  (1)..(516)
<223>  Rpb7

<400>  17
atg ttt ttt att aaa gac ctt tcg ctt aat att acc ctt cat ccg tcc         48
Met Phe Phe Ile Lys Asp Leu Ser Leu Asn Ile Thr Leu His Pro Ser           
1               5                   10                  15                

ttt ttc ggt cct cga atg aag caa tat cta aag aca aaa cta ttg gaa         96
Phe Phe Gly Pro Arg Met Lys Gln Tyr Leu Lys Thr Lys Leu Leu Glu           
            20                  25                  30                    

gag gtt gaa ggt tcg tgt acg ggt aaa ttc gga tat att ctt tgt gtc        144
Glu Val Glu Gly Ser Cys Thr Gly Lys Phe Gly Tyr Ile Leu Cys Val           
        35                  40                  45                        

cta gac tat gat aat ata gat att caa cgt ggg aga ata ttg ccc aca        192
Leu Asp Tyr Asp Asn Ile Asp Ile Gln Arg Gly Arg Ile Leu Pro Thr           
    50                  55                  60                            

gat ggg tcc gcc gag ttc aac gtg aaa tat aga gct gta gtt ttc aaa        240
Asp Gly Ser Ala Glu Phe Asn Val Lys Tyr Arg Ala Val Val Phe Lys           
65                  70                  75                  80            

cca ttt aaa ggg gaa gta gtg gac ggc aca gtc gtt tca tgt tct cag        288
Pro Phe Lys Gly Glu Val Val Asp Gly Thr Val Val Ser Cys Ser Gln           
                85                  90                  95                

cac ggg ttc gaa gtg caa gta ggt cca atg aaa gta ttt gtg aca aag        336
His Gly Phe Glu Val Gln Val Gly Pro Met Lys Val Phe Val Thr Lys           
            100                 105                 110                   

cat ctg atg cct caa gat tta acc ttt aat gcg ggt tca aac cca cca        384
His Leu Met Pro Gln Asp Leu Thr Phe Asn Ala Gly Ser Asn Pro Pro           
        115                 120                 125                       

tca tac caa agt tcc gag gat gtc atc acc ata aaa agt aga att aga        432
Ser Tyr Gln Ser Ser Glu Asp Val Ile Thr Ile Lys Ser Arg Ile Arg           
    130                 135                 140                           

gtt aaa att gaa ggt tgt atc agt caa gtg agt tct att cac gca atc        480
Val Lys Ile Glu Gly Cys Ile Ser Gln Val Ser Ser Ile His Ala Ile           
145                 150                 155                 160           

ggt agt atc aaa gaa gat tat ttg ggt gct att taa                        516
Gly Ser Ile Lys Glu Asp Tyr Leu Gly Ala Ile                               
                165                 170                                   


<210>  18
<211>  171
<212>  PRT
<213>  Saccharomyces cereviciae

<400>  18

Met Phe Phe Ile Lys Asp Leu Ser Leu Asn Ile Thr Leu His Pro Ser 
1               5                   10                  15      


Phe Phe Gly Pro Arg Met Lys Gln Tyr Leu Lys Thr Lys Leu Leu Glu 
            20                  25                  30          


Glu Val Glu Gly Ser Cys Thr Gly Lys Phe Gly Tyr Ile Leu Cys Val 
        35                  40                  45              


Leu Asp Tyr Asp Asn Ile Asp Ile Gln Arg Gly Arg Ile Leu Pro Thr 
    50                  55                  60                  


Asp Gly Ser Ala Glu Phe Asn Val Lys Tyr Arg Ala Val Val Phe Lys 
65                  70                  75                  80  


Pro Phe Lys Gly Glu Val Val Asp Gly Thr Val Val Ser Cys Ser Gln 
                85                  90                  95      


His Gly Phe Glu Val Gln Val Gly Pro Met Lys Val Phe Val Thr Lys 
            100                 105                 110         


His Leu Met Pro Gln Asp Leu Thr Phe Asn Ala Gly Ser Asn Pro Pro 
        115                 120                 125             


Ser Tyr Gln Ser Ser Glu Asp Val Ile Thr Ile Lys Ser Arg Ile Arg 
    130                 135                 140                 


Val Lys Ile Glu Gly Cys Ile Ser Gln Val Ser Ser Ile His Ala Ile 
145                 150                 155                 160 


Gly Ser Ile Lys Glu Asp Tyr Leu Gly Ala Ile 
                165                 170     


<210>  19
<211>  743
<212>  PRT
<213>  Pichia pastoris


<220>
<221>  peptide
<222>  (1)..(743)
<223>  CCR4

<400>  19

Met Asn Thr Ala Ser Gln Tyr Lys Ala Gln Gly Asn Gly Gln Gly Ile 
1               5                   10                  15      


Met Arg His Ala Pro Gln Pro Gln Gln Thr Gln Ile Pro Ser Gln Tyr 
            20                  25                  30          


Leu Leu Gln Gln Leu Gln Gly Ser His Ala Thr Asn Gln Pro Gln Thr 
        35                  40                  45              


Asp Ser Ser Asn Pro Ser Leu Leu Val Gln Ala Leu Phe Gln Gly Pro 
    50                  55                  60                  


Gln Ser His Gln Gly Gln Arg Ile Gly Asn Gln Gln Met Gln Pro His 
65                  70                  75                  80  


Val Val Leu Gly Ser Gln Thr Leu Gln Ser Ser Ser Val Val Pro Pro 
                85                  90                  95      


Gly Gln Asn Gln Pro Thr Ser Gln Ser Pro His Tyr Asp Thr Ile Gln 
            100                 105                 110         


Val Glu Asp Pro Thr Thr Phe His Trp Gln His Gln Val Gln Leu Val 
        115                 120                 125             


Gln Met Ser Arg Lys Cys Asn Gln Pro His Phe Tyr Ala Arg His Ala 
    130                 135                 140                 


Ala Val Ser Ser Arg Lys Leu Leu Gly Lys Asn Gly Asn Asn Asn Met 
145                 150                 155                 160 


Pro Gly Val Asp Gly Ser Gln Gly Asn Gln Pro Pro Asn Leu Leu Glu 
                165                 170                 175     


Ala Thr Lys Thr Leu Leu Met Ser Thr Asp Pro Thr Ala Glu Gln Ala 
            180                 185                 190         


Glu Ser Gly Glu Asn Lys Ser Ala Thr Asp Pro Leu Leu Trp Phe Lys 
        195                 200                 205             


Lys Leu Ser Asn Asp Thr Gln Asp Glu Asp Glu Glu Ile Asp Ala Ser 
    210                 215                 220                 


Thr Leu Lys Asp Pro Thr Arg Gln Leu Trp Arg Ala Leu Asp Leu Ser 
225                 230                 235                 240 


Gly Gln Gln Leu Leu His Leu Ser Glu Lys Leu Phe Arg Tyr Asp Phe 
                245                 250                 255     


Leu Thr Lys Leu Tyr Leu Asn Gly Asn Gly Leu Thr Glu Leu Pro Ser 
            260                 265                 270         


Ser Ile Arg Gln Leu Lys Ser Leu Thr Val Leu Asp Val Ser Gln Asn 
        275                 280                 285             


Leu Leu Ser Ser Phe Pro Pro Glu Leu Gly Ile Leu Phe Asn Leu Arg 
    290                 295                 300                 


Tyr Ile Tyr Ala Phe Asp Asn Arg Leu Thr Asp Ile Pro Phe Glu Phe 
305                 310                 315                 320 


Gly Asn Leu Tyr Glu Leu Glu Phe Leu Gly Ile Glu Gly Asn Val Asn 
                325                 330                 335     


Met Asn Pro Glu Tyr Val Asn Ile Leu Ala Lys Arg Gly Ser Arg Gly 
            340                 345                 350         


Leu Thr Ile His Leu Arg Asp Asn Ala Pro Arg Pro Thr Pro Pro Lys 
        355                 360                 365             


Ser Arg Gln Trp Ile Tyr Phe Ser Asn Asp Gly Glu Ile Ile Glu Glu 
    370                 375                 380                 


Gln Glu Tyr Arg Gln Gln Gln Thr Glu Asp Asp Ile Val Asn Thr Phe 
385                 390                 395                 400 


Thr Met Met Thr Tyr Asn Thr Leu Cys Gln His Tyr Ala Thr Lys Lys 
                405                 410                 415     


Met Tyr Arg Tyr Thr Pro Ser Trp Ala Leu Asp Trp Asp Tyr Arg Arg 
            420                 425                 430         


Glu Arg Leu Lys Glu Gln Ile Leu Asp Leu Gln Thr Asp Ile Ile Cys 
        435                 440                 445             


Leu Gln Glu Val Glu His Lys Thr Phe Asp Asp Phe Trp Gln Pro Ile 
    450                 455                 460                 


Met Leu Ser His Gly Tyr Lys Gly Ile Phe His Val Lys Ser Arg Ala 
465                 470                 475                 480 


Lys Thr Met Lys Glu Ser Ser Ala Tyr Lys Val Asp Gly Cys Ala Thr 
                485                 490                 495     


Phe Tyr Arg Thr Ser Lys Phe Gln Ala Val Glu Arg Lys His Phe Glu 
            500                 505                 510         


Tyr Gly Arg Ile Ala Met Ser Gln Asp Lys Phe Lys Lys Thr Glu Asp 
        515                 520                 525             


Leu Phe Asn Arg Phe Leu Asn Lys Asp Asn Ile Ala Ser Val Leu Ile 
    530                 535                 540                 


Leu Glu His Ile Pro Ser Gly Asn Lys Leu Val Val Ala Asn Thr His 
545                 550                 555                 560 


Leu His Trp Asp Pro Glu Phe Asn Asp Val Lys Thr Met Gln Val Gly 
                565                 570                 575     


Val Leu Leu Asp Glu Leu Gln Ala Val Ile Arg Lys His Leu Ser Pro 
            580                 585                 590         


Lys Asp Ile Thr Lys Val Pro Leu Leu Ile Cys Gly Asp Phe Asn Ser 
        595                 600                 605             


Lys Val His Ser Ala Val Tyr Gln Leu Phe Ser Gln Gly Thr Val Asp 
    610                 615                 620                 


Lys His Glu Asp Ile Ile Gly Arg Asp Tyr Gly Lys Phe Thr Glu Glu 
625                 630                 635                 640 


Gly Phe Arg His Pro Phe His Leu Gln Ser Ser Tyr Asp Ser Ile Gly 
                645                 650                 655     


Glu Leu Pro Tyr Thr Asn Val Ser Pro Thr Phe Thr Asp Val Ile Asp 
            660                 665                 670         


Tyr Ile Trp Tyr Ser Thr Pro Ser Leu Ser Val Lys Gly Val Leu Gly 
        675                 680                 685             


Gln Val Asp Pro Asp Tyr Ser Lys Asn Ile Ile Gly Phe Pro Asn Ala 
    690                 695                 700                 


Asp Phe Pro Ser Asp His Ile Pro Leu Leu Ser Thr Phe Met Phe Lys 
705                 710                 715                 720 


Lys Ser Ser Ala Pro Arg Pro Asp Thr Arg Val Asp Phe Arg Ser Asp 
                725                 730                 735     


Phe Arg Gly Ser Arg Lys Thr 
            740             


<210>  20
<211>  1118
<212>  PRT
<213>  Pichia pastoris


<220>
<221>  peptide
<222>  (1)..(1118)
<223>  Pan2

<400>  20

Met Glu Gly Trp Asn Glu Ile Asn Arg Val Pro Cys Met Arg Pro Ser 
1               5                   10                  15      


Phe Arg Ile Ser Asp Pro Thr Arg Gln Ser Val Pro Ser Ala Leu Leu 
            20                  25                  30          


Phe Asp Asp Ser His Asp Leu Thr Trp Ile Gly Ser Glu Asp Gly Phe 
        35                  40                  45              


Val Lys Ser Leu Ala Asp Gln Met Leu Thr Pro Tyr Thr Ser Phe Arg 
    50                  55                  60                  


Cys His Ser Ser Lys Val Leu Gln Leu Leu Asn Asn Lys Arg Gly Ile 
65                  70                  75                  80  


Leu Ser Leu Ser Glu Asn Ser Ile Lys Leu Thr Ser Arg Thr Gly Leu 
                85                  90                  95      


Cys Arg Met Asn Leu His Asp Ile Ser Val Asn His Ser Arg Ser Met 
            100                 105                 110         


Ala Tyr Thr Ser Asn Thr Glu Asn Glu Val Leu Leu Gly Gly Phe Gln 
        115                 120                 125             


Gly Lys Leu Val Lys Leu Asn Leu Met Arg Gly Glu Ile Ser Asp Thr 
    130                 135                 140                 


Ile Pro Tyr Asp Ala Pro Val Tyr Thr Met Ser Ala Asn Leu Ser Gln 
145                 150                 155                 160 


Ile Cys Leu Gly Arg Val Asp Gly Thr Val Asp Ile Leu Asp Pro Arg 
                165                 170                 175     


Ser Asn Asp Ile Ile Ala Ser Phe Ser Gly His Met Arg Thr Leu Ser 
            180                 185                 190         


Ser Met Asp Cys Arg Gly Asn Thr Leu Ile Thr Thr Gly Tyr Ser Val 
        195                 200                 205             


Arg Asn Gly Thr Phe Tyr Ala Asp Pro Ile Ala Asn Leu Tyr Asp Leu 
    210                 215                 220                 


Arg Thr Lys Ser Leu Met Pro Pro Val Thr Phe Pro Ala Gly Ala Ser 
225                 230                 235                 240 


Phe Val Lys Leu His Pro Lys Leu Pro Asn Val Ala Ile Leu Ala Ser 
                245                 250                 255     


Ser Ala Gly Leu Ile His Phe Val Asn Leu Tyr Ser Pro Met Asn Val 
            260                 265                 270         


Ser Leu Tyr Gln Ala Asp Val Gly Ser Tyr Met Asn Asn Phe Glu Val 
        275                 280                 285             


Ser Pro Ser Gly Asp Phe Met Gly Phe Thr Asp Ser Phe Gln Asn Val 
    290                 295                 300                 


His Leu Trp Ser Asn Ala Pro Asp Leu Ser Ser Ala His Ile Pro Asn 
305                 310                 315                 320 


Leu Ala Ser Ser Leu Glu Gln Ala Thr Met Leu Pro Pro Pro Ala Asn 
                325                 330                 335     


Ser Asp Ile Ile Pro Ala Asp Ser Asp Ser Thr Val Pro Leu Ser Ser 
            340                 345                 350         


Ile Gly Met Pro Tyr Tyr Asp Gln Pro Leu Leu Ser Asn Trp Pro Phe 
        355                 360                 365             


Asp Met Lys Phe Ser Leu Gly His Val Pro Lys Lys Ile Asn Pro Glu 
    370                 375                 380                 


Leu Ile Asn Ser Ser Lys Ser Val Val Glu Asn Tyr Asn Ala His Ala 
385                 390                 395                 400 


Phe Gly Gln Asn Asn Leu Pro Ser Gly Met Gly Val Asn Met Asn Ile 
                405                 410                 415     


Pro Gly Val Phe Lys Thr Asp Thr Asp Thr Arg Ile Ala Val Tyr Ser 
            420                 425                 430         


Arg Glu Lys Tyr Gly Pro Arg Asn Val Ala Gln Pro Tyr Tyr Arg Leu 
        435                 440                 445             


His Asp Arg Ser Asn Gln Lys Asn Lys Phe Val Pro Lys Phe Ile Ser 
    450                 455                 460                 


Glu Arg Leu Asp Val Asp Glu Lys Ser Ser Ser Thr Ala Glu Asn Thr 
465                 470                 475                 480 


Asn Ser Asp Phe Lys Asp Thr Leu Tyr Glu Gln Leu Phe Asp Cys Lys 
                485                 490                 495     


Pro His Ser Ser Gln Glu Val Pro Tyr Cys Phe Thr Lys Leu Asp Ile 
            500                 505                 510         


His Tyr Ser Lys Phe Gly Val His Asp Phe Asp Phe Glu Phe Phe Asn 
        515                 520                 525             


Lys Thr Asn Leu Ser Gly Leu Glu Ser His Val Gln Ser Ala Tyr Cys 
    530                 535                 540                 


Asn Ser Leu Leu Gln Leu Tyr Arg Phe Ala Pro Leu Met Phe Asn Tyr 
545                 550                 555                 560 


Ala Val Gly Ser Leu Ala Glu Glu Ser Val Asp Asn Asn Ser Leu Leu 
                565                 570                 575     


Thr Glu Leu Gly Phe Leu Phe Asp Met Met Val Lys Ala Ser Gly Gln 
            580                 585                 590         


His Ile Ala Pro Ser Asn Phe Gln Lys Met Leu Ser Lys Ile Pro Glu 
        595                 600                 605             


Ala Ser Arg Ile Leu Asp Ile Tyr Asn Asn Gly Tyr Asn Arg Asp Glu 
    610                 615                 620                 


His Ser Gln Arg Val Leu Ile Gln Ser Phe Asn Asn Phe Leu Leu Glu 
625                 630                 635                 640 


Arg Ile Ser Leu Asp Glu Met Asn Gln Ala Lys Ser Asn Ala Pro His 
                645                 650                 655     


Ile Phe Asn Thr Ile Met Gly Ile Pro Val Glu Cys Glu Phe Ile Gly 
            660                 665                 670         


Val Gly Cys Gly Leu Arg Lys Ile Gly Asn Thr Thr Leu Tyr Ser Leu 
        675                 680                 685             


Asp Val Arg His Pro Lys Thr Asn Asn Val Val Leu Asn Lys Lys Leu 
    690                 695                 700                 


Asn His Ser Ile Ile Pro Tyr Ile Glu Leu Ala Leu Ser Arg Asn Leu 
705                 710                 715                 720 


Ser Val Asn Met Ala Cys Asp Asn Cys Asn Glu Thr His Ala Phe Asp 
                725                 730                 735     


Thr Leu Tyr Thr Val Lys Asp Leu Pro Pro Ile Leu Ser Leu Asn Leu 
            740                 745                 750         


Glu Leu Thr Asn Gln Glu Leu Ser Glu Leu Lys Ala Ser Lys Thr Asn 
        755                 760                 765             


Trp Leu Ala Ser Glu Phe Tyr Ala Ser Met Asn Lys Gly Arg Ile Cys 
    770                 775                 780                 


Leu Lys Ser Ile Thr Thr Gly Phe Arg His Leu Lys Tyr Glu Leu Leu 
785                 790                 795                 800 


Gly Tyr Val Ala Gln Val Thr Asp Lys Glu Gly Asn Ser Asn Leu Val 
                805                 810                 815     


Thr Phe Val Lys Val Gly Lys Asp Glu Trp Phe Leu Phe Asn Asp Phe 
            820                 825                 830         


Leu Val Met Pro Ile Ser Glu His Glu Val Leu Asn Leu Asn Tyr Trp 
        835                 840                 845             


Trp Lys Lys Pro Val Ile Val Ile Tyr Lys Asn Ser Glu Ser Thr Asn 
    850                 855                 860                 


Met Phe Asp Tyr Glu Gly Trp Arg Gln Asn Leu Asn Gln Asp Ile Leu 
865                 870                 875                 880 


Tyr Arg Asp His Phe Ser Arg Gly Thr Arg Glu Gly Lys Ile Ile Glu 
                885                 890                 895     


Tyr Glu Leu Leu Thr Lys Glu Glu Ala Pro Gln Pro Gly Thr Leu Val 
            900                 905                 910         


Ala Ile Asp Ala Glu Phe Val Val Ile Glu Pro Glu Leu Val Glu Phe 
        915                 920                 925             


Asn Ser Asp Gly Thr Lys Lys Val Ile Arg Pro Leu Lys Asn Ser Leu 
    930                 935                 940                 


Ala Arg Val Ser Val Leu Arg Gly Asp Thr Gly Pro Lys Glu Gly Ile 
945                 950                 955                 960 


Pro Phe Ile Asp Asp Tyr Val Ile Ile Glu Glu Pro Ile Asn Asp Tyr 
                965                 970                 975     


Leu Thr Ser Trp Ser Gly Ile Glu Pro Asp Asp Leu Asn Leu Glu Lys 
            980                 985                 990         


Ser Lys Arg Ser Leu Thr Thr Leu  Gln Ala Val Tyr Arg  Lys Leu Trp 
        995                 1000                 1005             


Leu Leu  Leu Asn Leu Gly Cys  Ile Phe Val Gly His  Gly Leu Ile 
    1010                 1015                 1020             


Asn Asp  Phe Arg Thr Ile Asn  Leu Ser Val Pro Lys  Gln Gln Val 
    1025                 1030                 1035             


Arg Asp  Thr Ala Glu Leu Tyr  Phe Leu Lys Lys Glu  Lys Arg Lys 
    1040                 1045                 1050             


Leu Ser  Leu Lys Phe Leu Thr  Tyr Ala Val Leu Arg  Arg Glu Val 
    1055                 1060                 1065             


Gln Lys  Gly Asn His Asp Ser  Ile Glu Asp Ala Lys  Ala Ala Leu 
    1070                 1075                 1080             


Met Leu  Tyr Arg Lys Tyr Ile  Gln Leu Asn Asn Thr  Gly Glu Leu 
    1085                 1090                 1095             


Gln His  Thr Leu Glu Glu Val  Tyr Met Glu Gly Gln  Met Leu Ser 
    1100                 1105                 1110             


Phe Lys  Val Pro Thr 
    1115             


<210>  21
<211>  614
<212>  PRT
<213>  Pichia pastoris


<220>
<221>  peptide
<222>  (1)..(614)
<223>  Pan3

<400>  21

Met Ser Ala Asn Ser Asn Ser Thr Asn Thr Glu Asn His Asp Trp Ala 
1               5                   10                  15      


Lys Asp Ile Asp Cys Lys Asn Val Phe Ile His Gly Tyr Cys Lys Phe 
            20                  25                  30          


Glu Asn Lys Gly Cys Tyr Phe Lys His Pro Ser Asp Asp Ser Ile Glu 
        35                  40                  45              


Lys Asn Lys Glu Thr Lys Glu Thr Lys Ser Ser Ala Pro Leu Ser Ser 
    50                  55                  60                  


Gly Thr Glu Glu Gly Ser Ser Ile Arg Lys Lys Phe Ser Phe Glu Ala 
65                  70                  75                  80  


Pro Ser Phe Thr Pro Ser Gly Ser Val Ala Ser Leu Thr Asn Lys Phe 
                85                  90                  95      


Ser Thr Met Ser Pro Lys Leu Asp Glu Ile Pro Thr Phe Val Pro Thr 
            100                 105                 110         


Ser Gln Gln Thr Pro Lys Lys Pro Gln Gln Val Ser Ala Glu Ser Asn 
        115                 120                 125             


Asp Tyr Phe Ala Pro Pro Asn Ser Met Phe Thr Pro Ser Val Pro Ser 
    130                 135                 140                 


Asp Asn Ser Gln Pro Gly Leu Pro Leu Ser Ala Gln Phe Pro Gln His 
145                 150                 155                 160 


Gly Thr Thr Ala Pro His Asn Val Val Pro Glu Met Phe Tyr Ala Gln 
                165                 170                 175     


Ala Thr Gln Tyr Pro Leu Asn Tyr Asn Leu Tyr Ala Pro Pro Pro Pro 
            180                 185                 190         


Pro His Phe Asn Leu His Leu Asn Pro Asn Glu Arg Thr Val Ser Ser 
        195                 200                 205             


Phe Phe Ile Asp Asp Asn Leu Arg Glu Ser Leu Gln Lys Arg Asn Glu 
    210                 215                 220                 


Ala Cys Leu Gln Thr Phe Ser Asn Pro Ser Ile Pro Asp Ile Val Gly 
225                 230                 235                 240 


Val Tyr His Ser Leu Val Pro Leu Asn Asn Asn Phe Asp Asn Asn Ser 
                245                 250                 255     


Ala Arg Tyr Gly Ala Val Ser His Met Tyr Lys Ala Thr Ser Asn Lys 
            260                 265                 270         


Asp Ala Arg Leu Tyr Ala Leu Arg Arg Ile Glu Asn Val Asn Ile Thr 
        275                 280                 285             


Asp Lys Gln Ala Phe Lys Thr Ile Lys Ala Trp Ser Ser Ile Glu Asn 
    290                 295                 300                 


Ser Asn Ile Val Lys Val His Glu Ala Phe Thr Thr Thr Val Phe Gly 
305                 310                 315                 320 


Gly Asn Ser Leu Val Val Ala Tyr Asp Phe Tyr Gly Asn Ala Lys Thr 
                325                 330                 335     


Leu Ile Glu Ile His Phe Gln Asn Gln Pro Glu Leu Ile Thr Glu Gln 
            340                 345                 350         


His Leu Trp Ser Tyr Leu Ile Gln Leu Val Asn Ala Leu Asn Glu Val 
        355                 360                 365             


His Asp Lys Gly Leu Ala Val Arg Ser Ile Asp Leu Ser Lys Val Ile 
    370                 375                 380                 


Val Thr Asn Lys Asn Arg Ile Lys Leu Ser Gly Cys Gly Ile Val Asp 
385                 390                 395                 400 


Ile Leu Gln His Glu Asn Thr Glu Asp Ile Ala Gln Leu Gln Lys Lys 
                405                 410                 415     


Asp Leu Glu Leu Leu Ala Lys Leu Leu Tyr Asp Leu Ser Ile Thr Ser 
            420                 425                 430         


Ile Tyr Gly Ser Val Ser Phe Asp Asp Lys Thr Gln Asp Gln Ile Ile 
        435                 440                 445             


Asp His Leu Lys Phe Ser Asp Asp Tyr Lys Ala Thr Leu Lys Tyr Leu 
    450                 455                 460                 


Ile Ser Ala Asp Phe Asn Leu Lys Glu Ile Gln Thr Arg Ile Ala Pro 
465                 470                 475                 480 


Arg Leu Leu Asp Val Ile Asp Gly Leu Gln Asn Ser Asn Asp Phe Ile 
                485                 490                 495     


Glu Ser Gln Leu Ser Thr Glu Leu Glu Asn Ala Arg Leu Val Arg Leu 
            500                 505                 510         


Met Ser Lys Leu Ser Phe Leu His Gly Arg Pro Glu His Glu Gly Asp 
        515                 520                 525             


Pro Asn Trp Ser Glu Ser Ser Ala Asn Tyr Pro Leu Thr Leu Phe Val 
    530                 535                 540                 


Asp Tyr Val Tyr His Gln Val Asp Glu Arg Gly Tyr Pro Val Val Asp 
545                 550                 555                 560 


Leu Ala His Val Ile Thr Cys Leu Asn Lys Leu Asp Ala Gly Ile Glu 
                565                 570                 575     


Glu Arg Ile Leu Leu Val Ser Lys Asp Glu Lys Asn Cys Ile Ile Ile 
            580                 585                 590         


Ser Tyr Lys Glu Leu Lys Thr Leu Ile Glu Glu Gly Phe Asn Glu Leu 
        595                 600                 605             


Arg Met Arg Lys Asp Ile 
    610                 


<210>  22
<211>  186
<212>  PRT
<213>  Pichia pastoris


<220>
<221>  peptide
<222>  (1)..(186)
<223>  Rpb4

<400>  22

Met Asn Val Ser Thr Ser Thr Val Gly Ala Arg Arg Arg Arg Ala Lys 
1               5                   10                  15      


Gln Gln Val Asp Asp Glu Glu Asn Ala Thr Leu Leu Arg Leu Gly Pro 
            20                  25                  30          


Glu Phe Ala Leu Lys Gln Tyr Asp His Asp Gly Asn Glu His Asp Leu 
        35                  40                  45              


Ile Ala Leu Ser Leu Ser Glu Ser Arg Leu Leu Ile Arg Glu Ala Leu 
    50                  55                  60                  


Lys Ala Arg Ser Arg Ala Arg Asn Gly Gly Val Asp Ile Glu Ser Ser 
65                  70                  75                  80  


Asn Gly Glu Ile Asp Asp Asp Glu Leu Ala Lys Val Thr Ser Gly Ala 
                85                  90                  95      


Val Ala Asn Gly Val Val Lys Lys Thr Leu Asp Tyr Leu Asn Thr Phe 
            100                 105                 110         


Ala Arg Phe Lys Asp Glu Glu Thr Cys Thr Ala Val Asp Gln Leu Leu 
        115                 120                 125             


His Asn Ser Ser Asp Cys Ser Val Leu His Pro Phe Glu Ile Ala Gln 
    130                 135                 140                 


Leu Ser Ser Leu Gly Cys Glu Asp Val Asp Glu Ala Ile Thr Leu Ile 
145                 150                 155                 160 


Pro Ser Leu Ala Ala Lys Lys Glu Val Asn Leu Gln Arg Ile Leu Asp 
                165                 170                 175     


Glu Leu Asn Arg Leu Glu Asp Pro Tyr Lys 
            180                 185     


<210>  23
<211>  171
<212>  PRT
<213>  Pichia pastoris


<220>
<221>  peptide
<222>  (1)..(171)
<223>  Rpb7

<400>  23

Met Phe Phe Leu Lys Asp Leu Ser Leu Ile Leu Thr Leu His Pro Ser 
1               5                   10                  15      


Tyr Phe Gly Pro Gln Met Asn Gln Tyr Leu Arg Glu Lys Leu Leu Thr 
            20                  25                  30          


Asp Val Glu Gly Thr Cys Thr Gly Gln Phe Gly Tyr Ile Val Thr Val 
        35                  40                  45              


Leu Asp Gly Met Asn Ile Asp Val Gly Lys Gly Arg Ile Ile Pro Gly 
    50                  55                  60                  


Ser Gly Ser Ala Glu Phe Glu Val Lys Tyr Arg Ala Val Val Trp Lys 
65                  70                  75                  80  


Pro Phe Lys Gly Glu Val Val Asp Ala Ile Val Ser Asn Val Ser Pro 
                85                  90                  95      


Ile Gly Phe Phe Ala Asp Val Gly Pro Leu Asn Val Phe Val Ser Thr 
            100                 105                 110         


Arg Leu Ile Pro Asp Asn Leu Val Tyr Asn Pro Ser Asn Ser Pro Pro 
        115                 120                 125             


Ala Tyr Met Ser Asn Asp Glu Leu Ile Thr Lys Gly Ser Lys Val Arg 
    130                 135                 140                 


Leu Lys Val Val Gly Thr Arg Thr Asp Val Asn Glu Ile Tyr Ala Ile 
145                 150                 155                 160 


Gly Ser Ile Lys Glu Asp Phe Leu Gly Ala Ile 
                165                 170     


<210>  24
<211>  656
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  peptide
<222>  (1)..(656)
<223>  CCR4

<400>  24

Met Asn Gly Gly Gln Ala His Gln Arg Phe Gly Met Gln Ile Pro Lys 
1               5                   10                  15      


Phe Gln Ser Gln Ser His His Pro His Pro Ala Gln Gln Ala His His 
            20                  25                  30          


His Ala His His Asn Gln Ala Ser His Ser Ile Asn His Gln His Asn 
        35                  40                  45              


Phe Ser Ser Gly Ala Leu Ala Ala Ala Thr Pro His Phe Thr Pro Gly 
    50                  55                  60                  


Pro Leu Gln Asn Gly Ala His Val Asn Val Asp Glu Asp Ile Asp Glu 
65                  70                  75                  80  


Thr Met Asn Glu His Trp Gln Gln Gln Leu Gln Leu Ala Ala Glu Ser 
                85                  90                  95      


Arg Gln Ala Ser Ser Pro His Tyr Tyr Ala Arg Thr Val Ala Gln Gln 
            100                 105                 110         


Thr Lys Gly Ile Gln Ile Ala Pro Ser Gln Pro Glu Ser Gln Glu Asn 
        115                 120                 125             


Gly Ser Gly Asp Arg Asn Gly Leu Val Lys Ser Lys Pro Ala Pro Arg 
    130                 135                 140                 


Gln Gly Trp His Ala Leu Asp Phe Gly Gly Gln Gly Leu Arg Ala Leu 
145                 150                 155                 160 


Ala Thr Ser Leu Phe His Tyr Thr Phe Leu Glu Lys Leu Tyr Leu Asn 
                165                 170                 175     


His Asn Lys Leu Lys Thr Leu Pro Pro Ala Ile Gly Gln Leu Arg Lys 
            180                 185                 190         


Leu Thr His Leu Asp Leu Ser Ser Asn Asp Ile Ser Glu Leu Pro Glu 
        195                 200                 205             


Glu Ile Gly Met Leu Thr Ser Leu Lys Gln Leu Leu Leu Phe Asp Asn 
    210                 215                 220                 


Asn Ile Arg Thr Leu Pro Phe Glu Met Gly Tyr Leu Tyr Arg Leu Glu 
225                 230                 235                 240 


Met Leu Gly Ile Glu Gly Asn Pro Leu Asn Asp Val Leu Lys Ser Gln 
                245                 250                 255     


Ile Ile Lys Glu Gly Thr Lys Ala Leu Val Arg Tyr Leu Arg Glu Glu 
            260                 265                 270         


Met Pro Val His Leu Pro Pro Pro Asp Arg Asp Trp Ile Ile Leu Asp 
        275                 280                 285             


Glu Thr Ala Ser Ser Ser Asn Ser Pro Thr Glu Lys Ile Thr Val Leu 
    290                 295                 300                 


Ser His Asn Ala Leu Cys Asp Ser Ser Ala Thr Pro Ser His Phe Gly 
305                 310                 315                 320 


Tyr Thr Pro Ser Arg Val Leu Ser Trp Glu Phe Arg Arg Glu Leu Ile 
                325                 330                 335     


Leu Ser Glu Leu Arg Ser His Asp Ser Asp Ile Ile Cys Leu Gln Glu 
            340                 345                 350         


Ile Asp Gln Gly Ser Tyr Asn Gly Phe Phe Arg Glu Gln Leu Ala Tyr 
        355                 360                 365             


Asn Asp Tyr Lys Gly Val Tyr Trp Pro Arg Gly Arg Ala Met Gly Met 
    370                 375                 380                 


Gln Glu Glu Glu Ala Lys Ser Val Asp Gly Cys Ala Thr Phe Phe Lys 
385                 390                 395                 400 


Gly Ser Lys Phe Ile Leu Leu Asp Lys Gln Met Ile Asn Phe Gly Gln 
                405                 410                 415     


Thr Ala Val Arg Arg Pro Asp Ala Lys Gly Gln Asp Asp Ile Tyr Asn 
            420                 425                 430         


Arg Leu Trp Gln Lys Asp His Ile Ala Val Val Ile Phe Leu Glu Asn 
        435                 440                 445             


Arg Leu Thr Gly Ser Arg Phe Ile Val Val Asn Ala His Leu Tyr Trp 
    450                 455                 460                 


Asp Pro Ala Phe Lys Asp Val Lys Leu Ile Gln Thr Ala Ile Leu Met 
465                 470                 475                 480 


Glu Glu Ile Thr Lys Leu Ser Glu Lys Tyr Ala Lys Phe Pro Pro Cys 
                485                 490                 495     


Thr Asp Lys Thr Ala Phe Arg Phe Ser Glu Ala Glu Val Glu Tyr Ala 
            500                 505                 510         


Ser Gly Asp Gln Ile Pro Leu Phe Met Cys Gly Asp Phe Asn Ser Ala 
        515                 520                 525             


Pro Gly Ser Ala Ala Tyr Asn Leu Val Ala His Gly Arg Leu Thr Glu 
    530                 535                 540                 


Ser His Pro Asp Leu Glu Lys Arg Leu Tyr Gly Asn Leu Ser Arg Val 
545                 550                 555                 560 


Gly Met Thr His Pro Phe Lys Leu Lys Ser Ala Tyr Asn Ser Ile Gly 
                565                 570                 575     


Glu Leu Ser Phe Thr Asn Tyr Thr Pro Asp Phe Lys Asp Ile Leu Asp 
            580                 585                 590         


Tyr Ile Trp Tyr Thr Ser Asn Thr Leu His Val Ser Ala Leu Leu Gly 
        595                 600                 605             


Glu Val Asp Lys Glu Tyr Leu Gln Lys Val Pro Gly Phe Pro Asn Phe 
    610                 615                 620                 


His Phe Pro Ser Asp His Val Ala Leu Phe Ala Glu Phe Thr Val Lys 
625                 630                 635                 640 


Gly Lys Lys Gly Lys Val Val Glu Ala Asp Phe Gly Pro Gln Arg Asn 
                645                 650                 655     


<210>  25
<211>  1150
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  peptide
<222>  (1)..(1150)
<223>  Pan2

<400>  25

Met Glu Ala Asp Trp Asp Glu Leu Ser Arg Ile Pro Val Pro Ala Pro 
1               5                   10                  15      


Ser Val His Ala Leu Pro Thr Ile Ala Thr Ala Ile Ala Phe Asp Asp 
            20                  25                  30          


Val Met Glu Leu Leu Trp Gly Arg Ile Thr Ser Phe Phe Gly Pro Glu 
        35                  40                  45              


Leu Gln Arg Tyr Thr Ser Val Arg Ala His Pro Ala Thr Glu Pro Val 
    50                  55                  60                  


Arg Gln Ile Ile Phe His Asp Arg Gly Val Ile Ser Leu Ser Pro Lys 
65                  70                  75                  80  


Ser Val His Met Ile Thr Arg Arg Gly Leu Thr Gln Trp His Ile Ala 
                85                  90                  95      


His Glu Glu Met Thr Asp Leu Arg Cys Met Ser Phe Thr Ala Gln Thr 
            100                 105                 110         


Asn Arg Ile Ile Val Ala Gly Cys Gln Lys Ser Met Phe Thr Ile Asp 
        115                 120                 125             


Ile Asp Lys Gly Ile Ile Ile Asp Lys Leu His Thr Glu Tyr Asn Tyr 
    130                 135                 140                 


Thr Ile Met Lys Lys Ser Arg Tyr Leu Cys Ala Ala Thr Asp Thr Gly 
145                 150                 155                 160 


Ser Val Asn Ala Leu Ser Leu Asn Asp Phe Ser Val Val Lys Ser Trp 
                165                 170                 175     


Lys Ala His Gly Thr Ala Val Asn Asp Met Asp Ala Arg Asn Asp Leu 
            180                 185                 190         


Leu Val Thr Cys Gly Phe Ser Val Arg His Leu Gly Ser Pro Ile Val 
        195                 200                 205             


Asp Pro Leu Ala Asn Val Tyr Asp Leu Lys Thr Leu Ser Pro Leu Pro 
    210                 215                 220                 


Pro Ile Pro Phe His Ala Gly Ala Ala Tyr Val Arg Met His Pro Lys 
225                 230                 235                 240 


Leu His Thr Thr Ser Phe Val Ala Ser Gln Thr Gly Gln Leu Gln Val 
                245                 250                 255     


Val Asp Leu Met Asn Pro Asn Ala Ile Asn Leu Arg Gln Ala Asn Val 
            260                 265                 270         


Ser Phe Met Leu Gly Ile Asp Leu Ser Pro Ser Gly Glu Ala Leu Ala 
        275                 280                 285             


Ile Asn Asp Ala Glu Cys Ala Ile His Leu Trp Gly Ser Pro Ala Lys 
    290                 295                 300                 


Val His Phe Asn Glu Met Ser Lys Glu Ala Glu Phe Gly Asp Val Ala 
305                 310                 315                 320 


Pro Arg Pro Pro Thr Leu Asp Trp Ser Pro Glu Thr Pro Leu Ser Met 
                325                 330                 335     


Ile Gly Met Pro Tyr Tyr His Glu Arg Leu Phe Ser Ala Trp Pro Ser 
            340                 345                 350         


His Leu Val Phe Glu Val Gly Ser Pro Pro Pro Gln Val Asp Gln Ala 
        355                 360                 365             


Leu Ile Pro Tyr Leu Arg Pro Ala Glu Leu Gly His His Ala Pro Asn 
    370                 375                 380                 


Pro Lys Lys Thr Arg Arg Tyr Gln Val Glu Asn Thr Arg Ala Leu Ala 
385                 390                 395                 400 


Ser Ala Glu Pro Ala Leu Ile Ala Pro Lys Phe Leu Ser Glu Lys Ala 
                405                 410                 415     


Arg Glu Gln Asn Lys Ala Lys Ser Glu Gly Ala Ile Ser Asp Ala Ala 
            420                 425                 430         


Glu Ala Leu Ala Gly Ala Lys Ile Asn Gly Glu Thr Asp Asp Asp Pro 
        435                 440                 445             


Leu Leu Lys Tyr Ser Asn Val Glu Ile Lys Tyr Ser Arg Phe Gly Val 
    450                 455                 460                 


Asp Asp Phe Asp Phe Arg Phe Tyr Asn Gln Thr Thr Phe Ser Gly Leu 
465                 470                 475                 480 


Glu Thr His Ile Ala Asn Ser Phe Thr Asn Ala Leu Leu Gln Leu Phe 
                485                 490                 495     


Lys Phe Ile Pro Tyr Ile Arg Asn Val Ala Leu His His Ala Ala Ser 
            500                 505                 510         


Ser Cys Ile Phe Glu Thr Cys Leu Leu Cys Glu Met Gly Tyr Leu Phe 
        515                 520                 525             


Asp Met Leu Glu Lys Ala Ser Gly Gln Asn Cys Gln Ala Thr Asn Leu 
    530                 535                 540                 


Leu Lys Thr Phe Ser Ser Tyr Arg Glu Ala Ser Asn Leu Gly Leu Phe 
545                 550                 555                 560 


Glu Glu Asn Leu Thr Asn Lys Ser Leu Ser Ala Ala Ile Gln Ala Val 
                565                 570                 575     


Asn Arg Phe Phe Leu Gly Gln Ile Ser His Asp Phe Arg Met Ile Ser 
            580                 585                 590         


Pro Ser Ser Asp Asp Leu Asp His Arg Leu Ala Thr Val Ala Ser Glu 
        595                 600                 605             


Ser Ile Arg Cys Met Phe Cys Gln Asn Glu Ile Val Arg Pro Gly Asn 
    610                 615                 620                 


Ser Leu Val Asn Glu Leu Asn Tyr Pro Ala Ile Asp Ile Lys Gln Ala 
625                 630                 635                 640 


Arg Arg Asn Pro Ala Phe Arg Phe Ser Asn Ile Leu Arg Ala Ser Ile 
                645                 650                 655     


Glu Arg Glu Ala Gln Asn Arg Gly Trp Cys Asn Tyr Cys Arg Arg Tyr 
            660                 665                 670         


Gln Gln Val Ala Ile Arg Lys Ser Val His Arg Met Pro Gln Val Leu 
        675                 680                 685             


Met Leu Asn Ala Ala Leu Thr Asn Pro Ile Cys Arg Arg Leu Trp Ala 
    690                 695                 700                 


Ile Pro Gly Trp Leu Pro Glu Glu Val Gly Ile Val Ile Glu Gly Gly 
705                 710                 715                 720 


Gln Ile Leu Cys Phe Glu Gly Glu Asp Leu Lys Leu Arg Val Gln Ala 
                725                 730                 735     


Lys Met Pro Gly Leu Val Val Tyr Asp Leu Val Gly Leu Val Cys Glu 
            740                 745                 750         


Ile Asp Ile Pro Glu His Gln Lys Ala His Leu Val Ser Phe Ile Asn 
        755                 760                 765             


Val Ser Ile Ser Ser Arg Glu Pro Glu Thr Lys Asn Lys Trp His Leu 
    770                 775                 780                 


Phe Asn Asp Phe Leu Val Thr Glu Val Asp Lys Glu Glu Ala Leu Arg 
785                 790                 795                 800 


Phe Asn Gln Pro Trp Lys Ile Pro Cys Val Leu Ala Tyr Gln Val Gln 
                805                 810                 815     


Asp Gly Arg His Ala Met Asp Asp Thr Trp Lys Asp Ala Leu Asp Thr 
            820                 825                 830         


Thr Leu Leu Phe Arg Asp Trp Ser Leu Asn Gly Gly Arg Pro Val Glu 
        835                 840                 845             


Ser Arg Val Thr Leu Ser Glu Glu Glu Lys Pro Thr Pro Gly Thr Pro 
    850                 855                 860                 


Val Ala Leu Asp Thr Glu Phe Val Asp Leu Glu Lys Ala Glu Ile Asp 
865                 870                 875                 880 


Val Lys Ala Asp Gly Ser Gln Glu Ile Val Arg Pro Ser Lys Ser Gly 
                885                 890                 895     


Leu Ala Arg Val Ser Val Leu Arg Gly Ser Gly Ile Arg Glu Gly Val 
            900                 905                 910         


Pro Phe Ile Asp Asp Tyr Ile Thr Ile Lys Glu Asn Ile Val Asp Tyr 
        915                 920                 925             


Val Thr Gln Tyr Ser Gly Ile Lys Pro Gly Asp Leu Asp Pro Arg Val 
    930                 935                 940                 


Ser Gln His Asn Leu Val Pro Leu Lys Val Ala Tyr Lys Lys Leu Trp 
945                 950                 955                 960 


Leu Leu Leu Asn Leu Gly Cys Val Phe Val Gly His Gly Leu Ala Ser 
                965                 970                 975     


Asp Phe Arg Lys Val Asn Ile Gln Val Pro Lys Ser Gln Thr Val Asp 
            980                 985                 990         


Thr Gln Tyr Leu Phe Phe His Pro  Gly Lys Asn Arg Arg  Leu Ser Leu 
        995                 1000                 1005             


Arg Tyr  Leu Ala Trp Ala Val  Phe Lys Glu Tyr Ile  Gln Glu Glu 
    1010                 1015                 1020             


Pro Ala  Asp Asp Ser Gln Gly  His Asp Ser Ile Glu  Asp Ala Arg 
    1025                 1030                 1035             


Met Ala  Leu Arg Leu Trp Lys  Lys Phe Lys Glu Tyr  Glu Asp Ala 
    1040                 1045                 1050             


Gly Ile  Val Ser Gln Ile Leu  Glu Glu Ile Phe Arg  Glu Gly Ser 
    1055                 1060                 1065             


Lys Leu  Gly Phe Arg Pro Pro  Pro Arg Asn Gly Val  Pro Thr Val 
    1070                 1075                 1080             


Leu Ser  Arg Pro Gly Thr Ala  Val Thr Met Gln Asn  Asn Ser Gly 
    1085                 1090                 1095             


Arg Asn  Thr Pro Ser Thr Ser  Asp Val Ala Gly Ala  Ala Ala Ser 
    1100                 1105                 1110             


Ala Pro  Ala Thr Pro Arg Gln  Ala Phe Arg Arg Ser  Ile Ala Leu 
    1115                 1120                 1125             


Thr Pro  Ser Asn Gly Ser Phe  Ala Gly Pro Gly Thr  Gly Asp Phe 
    1130                 1135                 1140             


Phe Ser  Gly Ser Pro Leu Lys  
    1145                 1150 


<210>  26
<211>  642
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  peptide
<222>  (1)..(642)
<223>  Pan3

<400>  26

Met Ala Thr Thr Gly Lys Ser Ala Thr Leu Glu Asp Ala Arg His Gly 
1               5                   10                  15      


Thr Gly Ser Pro Lys Met Lys Gly Arg Gly Cys Ala Phe Asn His Asp 
            20                  25                  30          


Pro His Lys Val Asn Ser Gly Tyr Gln Ser Asp Ser Asn Lys Lys Arg 
        35                  40                  45              


Leu Asn Val Asp Ser Pro Ser Phe Thr Pro Ser Ile Leu Ser Ser Asn 
    50                  55                  60                  


Gly Ser Ser Pro Thr Ser Gln Ser Ala Thr Met Lys Lys Met Ala Thr 
65                  70                  75                  80  


Ile Ser Pro Lys Ala Ala Ser Ala Ala Pro Phe Gln Pro Arg Ser Ile 
                85                  90                  95      


Ser Ser Arg Ser Asn Ser Ser Thr Pro Thr Thr Arg Pro Gly Thr Met 
            100                 105                 110         


Thr Pro Asp Trp Ser Val Ala Glu Val Gln Glu Phe Val Pro Gln Gly 
        115                 120                 125             


Phe Asp Thr Ala His Ile Gly Ser Leu Gln Gly Asn Gly Thr Ala Gly 
    130                 135                 140                 


Val Pro Ser Thr Ser Ala Phe Asp Pro Phe Val Thr Ala Pro Asn Pro 
145                 150                 155                 160 


Leu Ser Ala Ala Asn Ala Val Gly Pro Val Gln Ala Asn Pro Phe Ser 
                165                 170                 175     


His Asp Thr Ala Ala Ala Leu Asn Gly Ala Ala Phe Phe Ala Asn Gln 
            180                 185                 190         


Ser Gly Phe Gln Gln Pro Val Gln Tyr His Met Tyr Ala Pro Ile Gly 
        195                 200                 205             


Pro His Ser Gln Asn Thr Leu Gly Tyr Gln Arg Asn Val His Asp Leu 
    210                 215                 220                 


Phe Leu Pro Asn Asp Leu Arg Glu Glu Met Gln Lys Lys Ala Ala Ala 
225                 230                 235                 240 


Thr Leu Gln Thr Leu Pro Asn Thr Gln Leu Pro Ala Gln Val Asp Tyr 
                245                 250                 255     


Phe His Ser Leu Val Pro Leu Asp Leu Asn His Gln Lys Asn Ala Thr 
            260                 265                 270         


Ile Phe Gly Phe Pro Ser Trp Val Tyr Lys Ala Gln Ser Ser Lys Asp 
        275                 280                 285             


Gly Asn Phe Tyr Ala Leu Arg Arg Leu Glu Gly Phe Arg Leu Thr Asn 
    290                 295                 300                 


Glu Lys Ala Ile Arg Ser Val Gln Ala Trp Lys Arg Val Cys Asn Gly 
305                 310                 315                 320 


Ser Val Val Thr Val His Asp Ala Phe Thr Ser Arg Ser Phe Gln Asp 
                325                 330                 335     


Ser Ser Leu Ile Phe Val Thr Asp Tyr His Pro Leu Ser Lys Thr Leu 
            340                 345                 350         


Ala Glu Gln His Leu Gly Ala Gly Asn Arg Phe Gln Gly Arg His Asn 
        355                 360                 365             


Thr His Ile Pro Glu Gln Val Leu Trp Gly Tyr Met Thr Gln Ile Ala 
    370                 375                 380                 


Asn Ala Leu Lys Ala Ile His Ala Ser Gln Leu Ala Ala Arg Ile Ile 
385                 390                 395                 400 


Asp Pro Ser Lys Ile Leu Leu Thr Gly Arg Asn Arg Ile Arg Leu Asn 
                405                 410                 415     


Ala Cys Ala Ile Met Asp Val Val Gln Phe Asp Thr Gln Arg Ser Leu 
            420                 425                 430         


Ala Glu Leu Gln Arg Gln Asp Leu Val Asn Phe Gly Gln Leu Ile Val 
        435                 440                 445             


Thr Leu Gly Ala Asn Gln Pro Asn Val Met His Asn Pro Thr Lys Ala 
    450                 455                 460                 


Met Glu His Phe Thr Arg Ala Tyr Thr Ala Gln Leu Lys Asn Ser Val 
465                 470                 475                 480 


Phe Trp Leu Leu Asn Gly Leu Gln Lys Asp Gln Glu Arg Asn Ile Asp 
                485                 490                 495     


Ile Phe Ile Thr Gly Ile Ser Ser Thr Leu Met Ser Thr Phe Asp Ser 
            500                 505                 510         


Ala Leu His Leu Asp Asp Gln Leu Thr Ser Asp Leu Ser Arg Glu Leu 
        515                 520                 525             


Glu Asn Gly Arg Leu Val Arg Leu Met Thr Lys Leu Asn Phe Val Asn 
    530                 535                 540                 


Glu Arg Pro Glu Tyr Glu His Asp Arg Gln Trp Ser Glu Asn Gly Glu 
545                 550                 555                 560 


Arg Tyr Phe Leu Lys Ile Phe Arg Asp Tyr Val Phe His Gln Val Asp 
                565                 570                 575     


Ala Gln Gly Asp Pro Val Val Asp Leu Gly His Val Leu Met Cys Leu 
            580                 585                 590         


Asn Lys Leu Asp Ala Gly Thr Asp Glu Lys Ile Thr Leu Ile Ser Arg 
        595                 600                 605             


Asp Glu Gln Ser Cys Phe Val Val Ser Tyr Lys Glu Leu Lys Lys Ala 
    610                 615                 620                 


Leu Glu Ser Ser Phe Gln Ala Leu Leu Lys Pro Ser Ala Ser Arg Arg 
625                 630                 635                 640 


Leu His 
        


<210>  27
<211>  148
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  peptide
<222>  (1)..(148)
<223>  Rpb4

<400>  27

Met Ser Val Gln Leu Pro Pro Ala Thr His Arg Lys Arg Thr Leu Pro 
1               5                   10                  15      


Gln Gly Glu Leu Glu Ala Ala Ser Thr Leu Lys Leu Gly Ala Asp Gln 
            20                  25                  30          


Asn Thr His Thr Leu Ser Leu Ser Glu Ala Arg Leu Val Ile Asn Lys 
        35                  40                  45              


Val Leu Glu Asn Lys Arg Arg Gly Gly Lys Lys Tyr Glu Glu Pro Glu 
    50                  55                  60                  


Asn Leu Thr Lys Thr Leu Asp Tyr Leu Glu Val Phe Ala Arg Phe Lys 
65                  70                  75                  80  


Asp Glu Glu Asn Ile Lys Ala Val Glu Arg Leu Leu Asn Ser His Thr 
                85                  90                  95      


Glu Leu Glu Met Phe Glu Arg Ser Gln Leu Gly Ser Leu Cys Cys Asp 
            100                 105                 110         


Asn Ala Glu Glu Ala Lys Ser Leu Ile Pro Ser Leu Gln His Lys Ile 
        115                 120                 125             


Ser Asp Gly Asp Leu Gln Glu Leu Leu Asp Glu Leu Thr Lys Leu Arg 
    130                 135                 140                 


Asn Phe Thr Glu 
145             


<210>  28
<211>  167
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  peptide
<222>  (1)..(167)
<223>  Rpb7

<400>  28

Met Phe Phe Leu Lys Glu Glu Val Lys Val Ile Thr Leu His Pro Ser 
1               5                   10                  15      


Tyr Phe Gly Pro Asn Met Arg Glu Tyr Leu Ile Asn Arg Leu Asn Glu 
            20                  25                  30          


Glu Glu Glu Gly Arg Cys Thr Gly Asp His Phe Val Ile Cys Val Met 
        35                  40                  45              


Asp Met Val Asp Ile Gly Glu Gly Arg Val Leu Pro Gly Ser Gly Gln 
    50                  55                  60                  


Ala Glu Tyr Thr Ile Lys Tyr Arg Ala Ile Ile Trp Lys Pro Phe Arg 
65                  70                  75                  80  


Gly Glu Thr Val Asp Ala Ile Val Thr Ser Val Lys Pro Thr Gly Ile 
                85                  90                  95      


Phe Thr Leu Ala Gly Pro Leu Ser Val Phe Ile Ala Arg Lys Asn Ile 
            100                 105                 110         


Pro Ser Asp Ile Lys Trp Glu Pro Asn Thr Val Pro Pro Gln Tyr Thr 
        115                 120                 125             


Asp His Ala Asp Gln Val Ile Glu Lys Gly Thr Ser Leu Arg Leu Lys 
    130                 135                 140                 


Ile Leu Gly Val Lys Pro Asp Val Ala Ala Ile Asn Ala Ile Gly Thr 
145                 150                 155                 160 


Ile Lys Glu Asp Tyr Leu Gly 
                165         


<210>  29
<211>  4886
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(4886)
<223>  Plasmid pLysS

<400>  29
gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt       60

gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt      120

ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga      180

tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga      240

aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt      300

ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc      360

ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat      420

ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt      480

gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg      540

acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact      600

ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa      660

aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc      720

actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc      780

ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa      840

agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc      900

agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct      960

cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg     1020

tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact     1080

gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg     1140

agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta     1200

gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt     1260

gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc     1320

gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca     1380

aaacgatctc aagaagatca tcttattaat cagataaaat atttctagat ttcagtgcaa     1440

tttatctctt caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca     1500

tgtttgacag cttatcatcg ataagcttta atgcggtagt ttatcacagt taaattgcta     1560

acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt     1620

caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga     1680

tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt     1740

gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc     1800

agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc     1860

cgtcctgtgg atccggccca ttggctgcct cccacacttg gatatgcctc ctcggagcct     1920

tatagaattg tttataagac ttgcgcatta tttgacctcc aatgcgaaca aagggaaacc     1980

gctgtggtct ccctttagtg agttcaatta attatccacg gtcagaagtg accagttcgt     2040

tcttctccca ccaacgctta aggtcgaacg aagggcaagc cttcggcgcc acctcatgat     2100

gggcgcgaag accagcgcct tcgtacttag ccagcagtgt gacaagcagt gagcgaaggg     2160

attgcatttg ggctggcgta aagttagcgt cgaacttacc tttatcgtcg ataccaccaa     2220

caaggcagac gccgatagag ttgtggttgt aacccttagc gtgagagcct acagccatct     2280

catctcgtcc tgcctccaca gtaccgtctc gcttgatgat aaagtggtat cccacatcga     2340

gccaaccctg ctctttgtgc cactggcgaa tctcacggac accaacattc tgacttggct     2400

tggtagccga gcagtgaaca aagattgcgt cagtagattc acgttgttta aactgtacac     2460

gagccattat ttctttcctc ctttcctttt taatctatca aaggggaccc ggatcctcta     2520

cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat     2580

cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt     2640

cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca     2700

tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct     2760

aatgcaggag tcgcataagg gagagcgtcg accgatgccc ttgagagcct tcaacccagt     2820

cagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga ctgtcttctt     2880

tatcatgcaa ctcgtaggac aggtgccggc agcgctctgg gtcattttcg gcgaggaccg     2940

ctttcgctgg agcgcgacga tgatcggcct gtcgcttgcg gtattcggaa tcttgcacgc     3000

cctcgctcaa gccttcgtca ctggtcccgc caccaaacgt ttcggcgaga agcaggccat     3060

tatcgccggc atggcggccg acgcgctggg ctacgtcttg ctggcgttcg cgacgcgagg     3120

ctggatggcc ttccccatta tgattcttct cgcttccggc ggcatcggga tgcccgcgtt     3180

gcaggccatg ctgtccaggc aggtagatga cgaccatcag ggacagcttc aaggatcgct     3240

cgcggctctt accagcctaa cttcgatcac tggaccgctg atcgtcacgg cgatttatgc     3300

cgcctcggcg agcacatgga acgggttggc atggattgta ggcgccgccc tataccttgt     3360

ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc acctcgacct gaatggaagc     3420

cggcggcacc tcgctaacgg attcaccact ccaagaattg gagccaatca attcttgcgg     3480

agaactgtga atgcgcaaac caacccttgg cagaacatat ccatcgcgtc cgccatctcc     3540

agcagccgca cgcggcgcat ctcgggcagc gttgggtcct ggccacgggt gcgcatgatc     3600

gtgctcctgt cgttgaggac ccggctaggc tggcggggtt gccttactgg ttagcagaat     3660

gaatcaccga tacgcgagcg aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga     3720

gcaacaacat gaatggtctt cggtttccgt gtttcgtaaa gtctggaaac gcggaagtcc     3780

cctacgtgct gctgaagttg cccgcaacag agagtggaac caaccggtga taccacgata     3840

ctatgactga gagtcaacgc catgagcggc ctcatttctt attctgagtt acaacagtcc     3900

gcaccgctgt ccggtagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt     3960

atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgcc caacagtccc     4020

ccggccacgg ggcctgccac catacccacg ccgaaacaag cgccctgcac cattatgttc     4080

cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg     4140

aagcgctaac cgtttttatc aggctctggg aggcagaata aatgatcata tcgtcaatta     4200

ttacctccac ggggagagcc tgagcaaact ggcctcaggc atttgagaag cacacggtca     4260

cactgcttcc ggtagtcaat aaaccggtaa accagcaata gacataagcg gctatttaac     4320

gaccctgccc tgaaccgacg accgggtcga atttgctttc gaatttctgc cattcatccg     4380

cttattatca cttattcagg cgtagcacca ggcgtttaag ggcaccaata actgccttaa     4440

aaaaattacg ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg     4500

ccgacatgga agccatcaca gacggcatga tgaacctgaa tcgccagcgg catcagcacc     4560

ttgtcgcctt gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata     4620

ttggccacgt ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac     4680

atattctcaa taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct     4740

tgcgaatata tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa     4800

aacgtttcag tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc     4860

agctcaccgt ctttcattgc catacg                                          4886


<210>  30
<211>  660
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(660)
<223>  Gene encoding chloramphenicol acetyltransferase, which confers 
       resistance to chloramphenicol

<400>  30
atg gag aaa aaa atc act gga tat acc acc gtt gat ata tcc caa tgg         48
Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp           
1               5                   10                  15                

cat cgt aaa gaa cat ttt gag gca ttt cag tca gtt gct caa tgt acc         96
His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr           
            20                  25                  30                    

tat aac cag acc gtt cag ctg gat att acg gcc ttt tta aag acc gta        144
Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val           
        35                  40                  45                        

aag aaa aat aag cac aag ttt tat ccg gcc ttt att cac att ctt gcc        192
Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala           
    50                  55                  60                            

cgc ctg atg aat gct cat ccg gaa ttc cgt atg gca atg aaa gac ggt        240
Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly           
65                  70                  75                  80            

gag ctg gtg ata tgg gat agt gtt cac cct tgt tac acc gtt ttc cat        288
Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His           
                85                  90                  95                

gag caa act gaa acg ttt tca tcg ctc tgg agt gaa tac cac gac gat        336
Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp           
            100                 105                 110                   

ttc cgg cag ttt cta cac ata tat tcg caa gat gtg gcg tgt tac ggt        384
Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly           
        115                 120                 125                       

gaa aac ctg gcc tat ttc cct aaa ggg ttt att gag aat atg ttt ttc        432
Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe           
    130                 135                 140                           

gtc tca gcc aat ccc tgg gtg agt ttc acc agt ttt gat tta aac gtg        480
Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val           
145                 150                 155                 160           

gcc aat atg gac aac ttc ttc gcc ccc gtt ttc acc atg ggc aaa tat        528
Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr           
                165                 170                 175               

tat acg caa ggc gac aag gtg ctg atg ccg ctg gcg att cag gtt cat        576
Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His           
            180                 185                 190                   

cat gcc gtc tgt gat ggc ttc cat gtc ggc aga atg ctt aat gaa tta        624
His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu           
        195                 200                 205                       

caa cag tac tgc gat gag tgg cag ggc ggg gcg taa                        660
Gln Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala                               
    210                 215                                               


<210>  31
<211>  219
<212>  PRT
<213>  synthetic

<400>  31

Met Glu Lys Lys Ile Thr Gly Tyr Thr Thr Val Asp Ile Ser Gln Trp 
1               5                   10                  15      


His Arg Lys Glu His Phe Glu Ala Phe Gln Ser Val Ala Gln Cys Thr 
            20                  25                  30          


Tyr Asn Gln Thr Val Gln Leu Asp Ile Thr Ala Phe Leu Lys Thr Val 
        35                  40                  45              


Lys Lys Asn Lys His Lys Phe Tyr Pro Ala Phe Ile His Ile Leu Ala 
    50                  55                  60                  


Arg Leu Met Asn Ala His Pro Glu Phe Arg Met Ala Met Lys Asp Gly 
65                  70                  75                  80  


Glu Leu Val Ile Trp Asp Ser Val His Pro Cys Tyr Thr Val Phe His 
                85                  90                  95      


Glu Gln Thr Glu Thr Phe Ser Ser Leu Trp Ser Glu Tyr His Asp Asp 
            100                 105                 110         


Phe Arg Gln Phe Leu His Ile Tyr Ser Gln Asp Val Ala Cys Tyr Gly 
        115                 120                 125             


Glu Asn Leu Ala Tyr Phe Pro Lys Gly Phe Ile Glu Asn Met Phe Phe 
    130                 135                 140                 


Val Ser Ala Asn Pro Trp Val Ser Phe Thr Ser Phe Asp Leu Asn Val 
145                 150                 155                 160 


Ala Asn Met Asp Asn Phe Phe Ala Pro Val Phe Thr Met Gly Lys Tyr 
                165                 170                 175     


Tyr Thr Gln Gly Asp Lys Val Leu Met Pro Leu Ala Ile Gln Val His 
            180                 185                 190         


His Ala Val Cys Asp Gly Phe His Val Gly Arg Met Leu Asn Glu Leu 
        195                 200                 205             


Gln Gln Tyr Cys Asp Glu Trp Gln Gly Gly Ala 
    210                 215                 


<210>  32
<211>  103
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(103)
<223>  cat promoter

<400>  32
tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt       60

attttttgag ttatcgagat tttcaggagc taaggaagct aaa                        103


<210>  33
<211>  545
<212>  DNA
<213>  synthetic


<220>
<221>  rep_origin
<222>  (1)..(545)
<223>  P15A ori

<400>  33
ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa aacgaaaaaa ccgccttgca       60

gggcggtttt tcgaaggttc tctgagctac caactctttg aaccgaggta actggcttgg      120

aggagcgcag tcaccaaaac ttgtcctttc agtttagcct taaccggcgc atgacttcaa      180

gactaactcc tctaaatcaa ttaccagtgg ctgctgccag tggtgctttt gcatgtcttt      240

ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac tgaacggggg      300

gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt gtcaggcgtg      360

gaatgagaca aacgcggcca taacagcgga atgacaccgg taaaccgaaa ggcaggaaca      420

ggagagcgca cgagggagcc gccaggggaa acgcctggta tctttatagt cctgtcgggt      480

ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat      540

ggaaa                                                                  545


<210>  34
<211>  34
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(34)
<223>  Phi 3.8 promoter

<400>  34
taattaattg aactcactaa agggagacca cagc                                   34


<210>  35
<211>  456
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(456)
<223>  T7 lysozyme

<400>  35
atg gct cgt gta cag ttt aaa caa cgt gaa tct act gac gca atc ttt         48
Met Ala Arg Val Gln Phe Lys Gln Arg Glu Ser Thr Asp Ala Ile Phe           
1               5                   10                  15                

gtt cac tgc tcg gct acc aag cca agt cag aat gtt ggt gtc cgt gag         96
Val His Cys Ser Ala Thr Lys Pro Ser Gln Asn Val Gly Val Arg Glu           
            20                  25                  30                    

att cgc cag tgg cac aaa gag cag ggt tgg ctc gat gtg gga tac cac        144
Ile Arg Gln Trp His Lys Glu Gln Gly Trp Leu Asp Val Gly Tyr His           
        35                  40                  45                        

ttt atc atc aag cga gac ggt act gtg gag gca gga cga gat gag atg        192
Phe Ile Ile Lys Arg Asp Gly Thr Val Glu Ala Gly Arg Asp Glu Met           
    50                  55                  60                            

gct gta ggc tct cac gct aag ggt tac aac cac aac tct atc ggc gtc        240
Ala Val Gly Ser His Ala Lys Gly Tyr Asn His Asn Ser Ile Gly Val           
65                  70                  75                  80            

tgc ctt gtt ggt ggt atc gac gat aaa ggt aag ttc gac gct aac ttt        288
Cys Leu Val Gly Gly Ile Asp Asp Lys Gly Lys Phe Asp Ala Asn Phe           
                85                  90                  95                

acg cca gcc caa atg caa tcc ctt cgc tca ctg ctt gtc aca ctg ctg        336
Thr Pro Ala Gln Met Gln Ser Leu Arg Ser Leu Leu Val Thr Leu Leu           
            100                 105                 110                   

gct aag tac gaa ggc gct gtg ctt cgc gcc cat cat gag gtg gcg ccg        384
Ala Lys Tyr Glu Gly Ala Val Leu Arg Ala His His Glu Val Ala Pro           
        115                 120                 125                       

aag gct tgc cct tcg ttc gac ctt aag cgt tgg tgg gag aag aac gaa        432
Lys Ala Cys Pro Ser Phe Asp Leu Lys Arg Trp Trp Glu Lys Asn Glu           
    130                 135                 140                           

ctg gtc act tct gac cgt gga taa                                        456
Leu Val Thr Ser Asp Arg Gly                                               
145                 150                                                   


<210>  36
<211>  151
<212>  PRT
<213>  synthetic

<400>  36

Met Ala Arg Val Gln Phe Lys Gln Arg Glu Ser Thr Asp Ala Ile Phe 
1               5                   10                  15      


Val His Cys Ser Ala Thr Lys Pro Ser Gln Asn Val Gly Val Arg Glu 
            20                  25                  30          


Ile Arg Gln Trp His Lys Glu Gln Gly Trp Leu Asp Val Gly Tyr His 
        35                  40                  45              


Phe Ile Ile Lys Arg Asp Gly Thr Val Glu Ala Gly Arg Asp Glu Met 
    50                  55                  60                  


Ala Val Gly Ser His Ala Lys Gly Tyr Asn His Asn Ser Ile Gly Val 
65                  70                  75                  80  


Cys Leu Val Gly Gly Ile Asp Asp Lys Gly Lys Phe Asp Ala Asn Phe 
                85                  90                  95      


Thr Pro Ala Gln Met Gln Ser Leu Arg Ser Leu Leu Val Thr Leu Leu 
            100                 105                 110         


Ala Lys Tyr Glu Gly Ala Val Leu Arg Ala His His Glu Val Ala Pro 
        115                 120                 125             


Lys Ala Cys Pro Ser Phe Asp Leu Lys Arg Trp Trp Glu Lys Asn Glu 
    130                 135                 140                 


Leu Val Thr Ser Asp Arg Gly 
145                 150     


<210>  37
<211>  29
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(29)
<223>  tet promoter

<400>  37
ttgacagctt atcatcgata agctttaat                                         29


<210>  38
<211>  8447
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(8447)
<223>  Plasmid pMax

<400>  38
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcaacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc     4800

cggatgtgcc gccagcgcca accccagctg aacctgccgc gcctgtcgta gccccagcac     4860

ctaaatctgc accggcaaca ccagccgctc ctgcccaacc tgggctgttg agccgcttct     4920

tcggcgcact gaaagcgctg ttcagcggtg gtgaagaaac caaaccgtcc gagcaaccaa     4980

caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca     5040

accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata     5100

atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg     5160

agagccgtca gcaggttgag gtaacggaaa aagcgcgtac caccgacgag cagcaagcgc     5220

cgcgtcgtga acgtagccgc cgccgtaatg atgataaacg tcaggcgcaa caagaagcga     5280

aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc     5340

cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg     5400

tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgtcgctgcc gaaccaattg     5460

ttcaggaagc gccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc     5520

aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc     5580

cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct     5640

atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc     5700

cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac     5760

aggttgaaga gcagcgcgaa caggaagaag tacatgtgca gccgatggtg actgaggtcc     5820

ctgtcgccgc cgctatcgaa ccggttgtta gcgcgccagt tgttgaagaa gtggccggtg     5880

tcgtagaagc ccccgttcag gttgccgaac cgcaaccgga agtggttgaa acgacgcatc     5940

ctgaagtgat tgctgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg     6000

ccgtagccca ggaagttgca gaacaagcag aaccggtggt tgaaccgcag gaagagacgg     6060

cagatattga agaagttgtc gaaactgctg aggttgtagt tgctgaacct gaagttgttg     6120

ctcaacctgc cgcgccagta gtcgctgaag tcgcagcaga agttgaaacg gtagctgcgg     6180

tcgaacctga ggtcaccgtt gagcataacc acgctaccgc gccaatgacg cgcgctccag     6240

caccggaata tgttccggag gcaccgcgtc acagtgactg gcagcgccct acttttgcct     6300

tcgaaggtaa aggtgccgca ggtggtcata cggcaacaca tcatgcctct gctgctcctg     6360

cgcgtccgca acctgttgag taaaggttac ctagaaatca tccttagcga aagctaagga     6420

ttttttttat ctgtaattaa ttgaactcac taaagggaga ccacagcggt ttccctttgt     6480

tcgcattgga ggtcaaataa tgcgcaagtc ttataaacaa ttctataagg ctccgaggag     6540

gcatatccaa gtgtgggagg cagccaatgg gccggatcca caggacgggt gtggtcgcca     6600

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa     6660

agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg     6720

ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc     6780

ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga     6840

tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact     6900

gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattacaac     6960

ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc     7020

tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta     7080

atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga     7140

gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc     7200

ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc     7260

agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt     7320

accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga     7380

gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca     7440

gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag     7500

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca     7560

gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct     7620

ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa     7680

gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg     7740

gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca     7800

catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg     7860

ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca     7920

ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact     7980

aaatcagtaa gttggcagca tcacccgacg cactttgcgc cgaataaata cctgtgacgg     8040

aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg     8100

ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata     8160

atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg     8220

aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc     8280

gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc     8340

agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg     8400

cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattc                   8447


<210>  39
<211>  3186
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(3186)
<223>  rne(D346N) encoding mutant RNase E of E.coli comprising a single 
       point mutation: D346N

<400>  39
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc aac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agc       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtc tgc ccg cgc tgc tcc ggt acc ggt acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tat ctg ctg aat gaa aaa cgt tct gcg gtg aat gcc att gaa acg       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtt cgc tgc gtg att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg cgt aaa ggg gaa gaa acg ccg       1488
Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro           
                485                 490                 495               

acc tta agc tac atg ctg ccg aag ctg cat gaa gaa gcg atg gcg ctg       1536
Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu           
            500                 505                 510                   

ccg tct gaa gaa gag ttc gct gaa cgt aag cgt ccg gaa caa cct gcg       1584
Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala           
        515                 520                 525                       

ctg gca acc ttt gcc atg ccg gat gtg ccg cca gcg cca acc cca gct       1632
Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala           
    530                 535                 540                           

gaa cct gcc gcg cct gtc gta gcc cca gca cct aaa tct gca ccg gca       1680
Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala           
545                 550                 555                 560           

aca cca gcc gct cct gcc caa cct ggg ctg ttg agc cgc ttc ttc ggc       1728
Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly           
                565                 570                 575               

gca ctg aaa gcg ctg ttc agc ggt ggt gaa gaa acc aaa ccg tcc gag       1776
Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu           
            580                 585                 590                   

caa cca aca ccg aaa gca gaa gcg aaa ccg gaa cgt caa cag gat cgt       1824
Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg           
        595                 600                 605                       

cgc aag cct cgt cag aac aac cgc cgt gac cgt aat gag cgc cgc gac       1872
Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp           
    610                 615                 620                           

acc cgt agt gaa cgt act gaa ggc agc gat aat cgc gaa gaa aac cgt       1920
Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg           
625                 630                 635                 640           

cgt aat cgt cgc cag gca cag cag cag act gcc gag acg cgt gag agc       1968
Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser           
                645                 650                 655               

cgt cag cag gtt gag gta acg gaa aaa gcg cgt acc acc gac gag cag       2016
Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln           
            660                 665                 670                   

caa gcg ccg cgt cgt gaa cgt agc cgc cgc cgt aat gat gat aaa cgt       2064
Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg           
        675                 680                 685                       

cag gcg caa caa gaa gcg aag gcg ctg aat gtt gaa gag caa tct gtt       2112
Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val           
    690                 695                 700                           

cag gaa acc gaa cag gaa gaa cgt gta cgt ccg gtt cag ccg cgt cgt       2160
Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg           
705                 710                 715                 720           

aaa cag cgt cag ctc aat cag aaa gtg cgt tac gag caa agc gta gcc       2208
Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala           
                725                 730                 735               

gaa gaa gcg gta gtc gca ccg gtg gtt gaa gaa act gtc gct gcc gaa       2256
Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu           
            740                 745                 750                   

cca att gtt cag gaa gcg cca gct cca cgc aca gaa ctg gtg aaa gtc       2304
Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val           
        755                 760                 765                       

ccg ctg cca gtc gta gcg caa act gca cca gaa cag caa gaa gag aac       2352
Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn           
    770                 775                 780                           

aat gct gat aac cgt gac aac ggt ggc atg ccg cgt cgt tct cgc cgc       2400
Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg           
785                 790                 795                 800           

tcg cct cgt cac ctg cgc gta agt ggt cag cgt cgt cgt cgc tat cgt       2448
Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg           
                805                 810                 815               

gac gag cgt tat cca acc cag tcg cca atg ccg ttg acc gta gcg tgc       2496
Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys           
            820                 825                 830                   

gcg tct ccg gaa ctg gcc tct ggc aaa gtc tgg atc cgc tat cca att       2544
Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile           
        835                 840                 845                       

gta cgt ccg caa gat gta cag gtt gaa gag cag cgc gaa cag gaa gaa       2592
Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu           
    850                 855                 860                           

gta cat gtg cag ccg atg gtg act gag gtc cct gtc gcc gcc gct atc       2640
Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile           
865                 870                 875                 880           

gaa ccg gtt gtt agc gcg cca gtt gtt gaa gaa gtg gcc ggt gtc gta       2688
Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val           
                885                 890                 895               

gaa gcc ccc gtt cag gtt gcc gaa ccg caa ccg gaa gtg gtt gaa acg       2736
Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr           
            900                 905                 910                   

acg cat cct gaa gtg att gct gcc gcg gta act gaa cag ccg cag gtg       2784
Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val           
        915                 920                 925                       

att acc gag tct gat gtt gcc gta gcc cag gaa gtt gca gaa caa gca       2832
Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala           
    930                 935                 940                           

gaa ccg gtg gtt gaa ccg cag gaa gag acg gca gat att gaa gaa gtt       2880
Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val           
945                 950                 955                 960           

gtc gaa act gct gag gtt gta gtt gct gaa cct gaa gtt gtt gct caa       2928
Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln           
                965                 970                 975               

cct gcc gcg cca gta gtc gct gaa gtc gca gca gaa gtt gaa acg gta       2976
Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val           
            980                 985                 990                   

gct gcg gtc gaa cct gag gtc acc  gtt gag cat aac cac  gct acc gcg     3024
Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala         
        995                 1000                 1005                     

cca atg  acg cgc gct cca gca  ccg gaa tat gtt ccg  gag gca ccg        3069
Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro            
    1010                 1015                 1020                        

cgt cac  agt gac tgg cag cgc  cct act ttt gcc ttc  gaa ggt aaa        3114
Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys            
    1025                 1030                 1035                        

ggt gcc  gca ggt ggt cat acg  gca aca cat cat gcc  tct gct gct        3159
Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala            
    1040                 1045                 1050                        

cct gcg  cgt ccg caa cct gtt  gag taa                                 3186
Pro Ala  Arg Pro Gln Pro Val  Glu                                         
    1055                 1060                                             


<210>  40
<211>  1061
<212>  PRT
<213>  E. coli

<400>  40

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu 
            580                 585                 590         


Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg 
        595                 600                 605             


Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp 
    610                 615                 620                 


Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg 
625                 630                 635                 640 


Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser 
                645                 650                 655     


Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln 
            660                 665                 670         


Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg 
        675                 680                 685             


Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val 
    690                 695                 700                 


Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg 
705                 710                 715                 720 


Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala 
                725                 730                 735     


Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu 
            740                 745                 750         


Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val 
        755                 760                 765             


Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn 
    770                 775                 780                 


Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg 
785                 790                 795                 800 


Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg 
                805                 810                 815     


Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys 
            820                 825                 830         


Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile 
        835                 840                 845             


Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu 
    850                 855                 860                 


Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile 
865                 870                 875                 880 


Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val 
                885                 890                 895     


Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr 
            900                 905                 910         


Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val 
        915                 920                 925             


Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala 
    930                 935                 940                 


Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val 
945                 950                 955                 960 


Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln 
                965                 970                 975     


Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val 
            980                 985                 990         


Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala 
        995                 1000                 1005             


Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro 
    1010                 1015                 1020             


Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys 
    1025                 1030                 1035             


Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala 
    1040                 1045                 1050             


Pro Ala  Arg Pro Gln Pro Val  Glu 
    1055                 1060     


<210>  41
<211>  119
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(119)
<223>  rhaBAD promoter

<400>  41
caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc       60

ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgta       119


<210>  42
<211>  54
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(53)
<223>  BCD

<400>  42
atgaaagcaa ttttcgtact gaaacatctt aatcatgcta aggaggtttt ctaa             54


<210>  43
<211>  41
<212>  DNA
<213>  synthetic


<220>
<221>  terminator
<222>  (1)..(41)
<223>  rrnc terminator

<400>  43
agaaatcatc cttagcgaaa gctaaggatt ttttttatct g                           41


<210>  44
<211>  48
<212>  DNA
<213>  synthetic


<220>
<221>  terminator
<222>  (1)..(48)
<223>  T7 terminator

<400>  44
ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttg                    48


<210>  45
<211>  7855
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(7855)
<223>  Plasmid pMax-noLysS

<400>  45
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt ttaattaagc ggccgcggcg     2400

cgcccgtcga cgcgcgcgcg gctgaggata cctcagccac cacaattcag caaattgtga     2460

acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga     2520

aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcttttt aagaaggaga     2580

ctatatatga aagcaatttt cgtactgaaa catcttaatc atgctaagga ggttttctaa     2640

tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg     2700

ggcagcgtct gtatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca     2760

tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg     2820

gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta     2880

actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca     2940

ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca     3000

gtctggcggg tagctatctg gttctgatgc cgaacaaccc gcgcgcgggt ggcatttctc     3060

gccgtatcga aggcgacgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc     3120

cggaaggcat ggggcttatc gtgcgcaccg ctggcgtcgg caaatctgct gaggcgctgc     3180

aatgggattt aagcttccgt ctgaaacact gggaagccat caaaaaagcc gctgaaagcc     3240

gcccggcccc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt     3300

acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac     3360

gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtacaccg     3420

gcgagatccc gctgttcagc cactaccaga tcgagtcaca gatcgagtcc gccttccagc     3480

gtgaagttcg tctgccgtct ggtggttcca ttgttatcga cagcaccgaa gcgttaacgg     3540

ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta     3600

acactaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg     3660

gcctgattgt tatcaacttc atcgacatga cgccagtacg ccaccagcgt gcggtagaaa     3720

accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc     3780

gctttggcct gctggaaatg tcccgtcagc gcctgagccc atcactgggt gaatccagcc     3840

atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc     3900

tctctattct gcgtctgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg     3960

ccattgttcc tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg     4020

ccattgaaac gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa     4080

ccccgcacta ccacgtgctg cgcgtgcgta aaggggaaga aacgccgacc ttaagctaca     4140

tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac     4200

gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat gccggatgtg ccgccagcgc     4260

caaccccagc tgaacctgcc gcgcctgtcg tagccccagc acctaaatct gcaccggcaa     4320

caccagccgc tcctgcccaa cctgggctgt tgagccgctt cttcggcgca ctgaaagcgc     4380

tgttcagcgg tggtgaagaa accaaaccgt ccgagcaacc aacaccgaaa gcagaagcga     4440

aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa caaccgccgt gaccgtaatg     4500

agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc     4560

gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggttg     4620

aggtaacgga aaaagcgcgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc     4680

gccgccgtaa tgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag     4740

agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta     4800

aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag     4860

tcgcaccggt ggttgaagaa actgtcgctg ccgaaccaat tgttcaggaa gcgccagctc     4920

cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc     4980

aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct     5040

cgcctcgtca cctgcgcgta agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc     5100

caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca     5160

aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaggttgaa gagcagcgcg     5220

aacaggaaga agtacatgtg cagccgatgg tgactgaggt ccctgtcgcc gccgctatcg     5280

aaccggttgt tagcgcgcca gttgttgaag aagtggccgg tgtcgtagaa gcccccgttc     5340

aggttgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgctgccg     5400

cggtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg     5460

cagaacaagc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg     5520

tcgaaactgc tgaggttgta gttgctgaac ctgaagttgt tgctcaacct gccgcgccag     5580

tagtcgctga agtcgcagca gaagttgaaa cggtagctgc ggtcgaacct gaggtcaccg     5640

ttgagcataa ccacgctacc gcgccaatga cgcgcgctcc agcaccggaa tatgttccgg     5700

aggcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg     5760

caggtggtca tacggcaaca catcatgcct ctgctgctcc tgcgcgtccg caacctgttg     5820

agtaaaggtt acctagaaat catccttagc gaaagctaag gatttttttt atctgggttt     5880

ccctttgttc gcattggagg tcaaataatg cgcaagtctt ataaacaatt ctataaggct     5940

ccgaggaggc atatccaagt gtgggaggca gccaatgggc cggatccaca ggacgggtgt     6000

ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg     6060

gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc     6120

atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg     6180

caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt     6240

gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca     6300

atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga     6360

attacaactt atatcgtatg gggctgactt caggtgctac atttgaagag ataaattgca     6420

ctgaaatcta gaaatatttt atctgattaa taagatgatc ttcttgagat cgttttggtc     6480

tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggcggt ttttcgaagg     6540

ttctctgagc taccaactct ttgaaccgag gtaactggct tggaggagcg cagtcaccaa     6600

aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac tcctctaaat     6660

caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt ggactcaaga     6720

cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc     6780

agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag acaaacgcgg     6840

ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga     6900

gccgccaggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca ccactgattt     6960

gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc tatggaaaaa cggctttgcc     7020

gcggccctct cacttccctg ttaagtatct tcctggcatc ttccaggaaa tctccgcccc     7080

gttcgtaagc catttccgct cgccgcagtc gaacgaccga gcgtagcgag tcagtgagcg     7140

aggaagcgga atatatcctg tatcacatat tctgctgacg caccggtgca gccttttttc     7200

tcctgccaca tgaagcactt cactgacacc ctcatcagtg ccaacatagt aagccagtat     7260

acactccgct agcgctgatg tccggcggtg cttttgccgt tacgcaccac cccgtcagta     7320

gctgaacagg agggacagct gatagaaaca gaagccactg gagcacctca aaaacaccat     7380

catacactaa atcagtaagt tggcagcatc acccgacgca ctttgcgccg aataaatacc     7440

tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa     7500

gccctgggcc aacttttggc gaaaatgaga cgttgatcgg cacgtaagag gttccaactt     7560

tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg     7620

agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca     7680

atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca     7740

gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt     7800

ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattc          7855


<210>  46
<211>  5144
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(5144)
<223>  Plasmid pET28a+

<400>  46
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag accagtttct agcataaccc cttggggcct ctaaacgggt      120

cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc      180

tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt      240

gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc      300

ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta      360

cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc      420

tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg      480

ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt      540

ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat      600

tttaacaaaa tattaacgtt tacaatttca ggtggcactt ttcggggaaa tgtgcgcgga      660

acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc      720

ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat      780

accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca      840

taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc      900

tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac      960

tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca     1020

gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg     1080

cgcctgagcg agacgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga     1140

atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata     1200

ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc     1260

atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt     1320

tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa     1380

caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac     1440

attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg     1500

cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat     1560

gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact     1620

gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg     1680

taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc     1740

aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata     1800

ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta     1860

catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc     1920

ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg     1980

ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac     2040

agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg     2100

taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt     2160

atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct     2220

cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg     2280

ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata     2340

accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca     2400

gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc     2460

tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc     2520

atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga     2580

cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac     2640

agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg     2700

aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg     2760

tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt     2820

ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg     2880

taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg     2940

atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg     3000

gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt     3060

aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac     3120

ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag     3180

accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc     3240

tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc     3300

ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc     3360

tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc     3420

aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc     3480

tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag     3540

acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg     3600

ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca     3660

ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat     3720

taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt     3780

tcttttcacc agtgagacgg gcaacagctg attgcccttc accgcctggc cctgagagag     3840

ttgcagcaag cggtccacgc tggtttgccc cagcaggcga aaatcctgtt tgatggtggt     3900

taacggcggg atataacatg agctgtcttc ggtatcgtcg tatcccacta ccgagatatc     3960

cgcaccaacg cgcagcccgg actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc     4020

gttggcaacc agcatcgcag tgggaacgat gccctcattc agcatttgca tggtttgttg     4080

aaaaccggac atggcactcc agtcgccttc ccgttccgct atcggctgaa tttgattgcg     4140

agtgagatat ttatgccagc cagccagacg cagacgcgcc gagacagaac ttaatgggcc     4200

cgctaacagc gcgatttgct ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt     4260

accgtcttca tgggagaaaa taatactgtt gatgggtgtc tggtcagaga catcaagaaa     4320

taacgccgga acattagtgc aggcagcttc cacagcaatg gcatcctggt catccagcgg     4380

atagttaatg atcagcccac tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca     4440

ggcttcgacg ccgcttcgtt ctaccatcga caccaccacg ctggcaccca gttgatcggc     4500

gcgagattta atcgccgcga caatttgcga cggcgcgtgc agggccagac tggaggtggc     4560

aacgccaatc agcaacgact gtttgcccgc cagttgttgt gccacgcggt tgggaatgta     4620

attcagctcc gccatcgccg cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc     4680

ctggttcacc acgcgggaaa cggtctgata agagacaccg gcatactctg cgacatcgta     4740

taacgttact ggtttcacat tcaccaccct gaattgactc tcttccgggc gctatcatgc     4800

cataccgcga aaggttttgc gccattcgat ggtgtccggg atctcgacgc tctcccttat     4860

gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg     4920

caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca     4980

ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat     5040

cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca     5100

cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat                      5144


<210>  47
<211>  19
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(19)
<223>  T7 promoter

<400>  47
taatacgact cactatagg                                                    19


<210>  48
<211>  25
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  lac operator

<400>  48
ggaattgtga gcggataaca attcc                                             25


<210>  49
<211>  456
<212>  DNA
<213>  synthetic


<220>
<221>  rep_origin
<222>  (1)..(456)
<223>  f1 ori

<400>  49
acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg       60

ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca      120

cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta      180

gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc      240

catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg      300

gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat      360

aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta      420

acgcgaattt taacaaaata ttaacgttta caattt                                456


<210>  50
<211>  816
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(816)
<223>  KanR

<400>  50
atg agc cat att caa cgg gaa acg tct tgc tct agg ccg cga tta aat         48
Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn           
1               5                   10                  15                

tcc aac atg gat gct gat tta tat ggg tat aaa tgg gct cgc gat aat         96
Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn           
            20                  25                  30                    

gtc ggg caa tca ggt gcg aca atc tat cga ttg tat ggg aag ccc gat        144
Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp           
        35                  40                  45                        

gcg cca gag ttg ttt ctg aaa cat ggc aaa ggt agc gtt gcc aat gat        192
Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp           
    50                  55                  60                            

gtt aca gat gag atg gtc aga cta aac tgg ctg acg gaa ttt atg cct        240
Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro           
65                  70                  75                  80            

ctt ccg acc atc aag cat ttt atc cgt act cct gat gat gca tgg tta        288
Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu           
                85                  90                  95                

ctc acc act gcg atc ccc ggg aaa aca gca ttc cag gta tta gaa gaa        336
Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu           
            100                 105                 110                   

tat cct gat tca ggt gaa aat att gtt gat gcg ctg gca gtg ttc ctg        384
Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu           
        115                 120                 125                       

cgc cgg ttg cat tcg att cct gtt tgt aat tgt cct ttt aac agc gat        432
Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp           
    130                 135                 140                           

cgc gta ttt cgt ctc gct cag gcg caa tca cga atg aat aac ggt ttg        480
Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu           
145                 150                 155                 160           

gtt gat gcg agt gat ttt gat gac gag cgt aat ggc tgg cct gtt gaa        528
Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu           
                165                 170                 175               

caa gtc tgg aaa gaa atg cat aaa ctt ttg cca ttc tca ccg gat tca        576
Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser           
            180                 185                 190                   

gtc gtc act cat ggt gat ttc tca ctt gat aac ctt att ttt gac gag        624
Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu           
        195                 200                 205                       

ggg aaa tta ata ggt tgt att gat gtt gga cga gtc gga atc gca gac        672
Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp           
    210                 215                 220                           

cga tac cag gat ctt gcc atc cta tgg aac tgc ctc ggt gag ttt tct        720
Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser           
225                 230                 235                 240           

cct tca tta cag aaa cgg ctt ttt caa aaa tat ggt att gat aat cct        768
Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro           
                245                 250                 255               

gat atg aat aaa ttg cag ttt cat ttg atg ctc gat gag ttt ttc taa        816
Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe               
            260                 265                 270                   


<210>  51
<211>  271
<212>  PRT
<213>  synthetic

<400>  51

Met Ser His Ile Gln Arg Glu Thr Ser Cys Ser Arg Pro Arg Leu Asn 
1               5                   10                  15      


Ser Asn Met Asp Ala Asp Leu Tyr Gly Tyr Lys Trp Ala Arg Asp Asn 
            20                  25                  30          


Val Gly Gln Ser Gly Ala Thr Ile Tyr Arg Leu Tyr Gly Lys Pro Asp 
        35                  40                  45              


Ala Pro Glu Leu Phe Leu Lys His Gly Lys Gly Ser Val Ala Asn Asp 
    50                  55                  60                  


Val Thr Asp Glu Met Val Arg Leu Asn Trp Leu Thr Glu Phe Met Pro 
65                  70                  75                  80  


Leu Pro Thr Ile Lys His Phe Ile Arg Thr Pro Asp Asp Ala Trp Leu 
                85                  90                  95      


Leu Thr Thr Ala Ile Pro Gly Lys Thr Ala Phe Gln Val Leu Glu Glu 
            100                 105                 110         


Tyr Pro Asp Ser Gly Glu Asn Ile Val Asp Ala Leu Ala Val Phe Leu 
        115                 120                 125             


Arg Arg Leu His Ser Ile Pro Val Cys Asn Cys Pro Phe Asn Ser Asp 
    130                 135                 140                 


Arg Val Phe Arg Leu Ala Gln Ala Gln Ser Arg Met Asn Asn Gly Leu 
145                 150                 155                 160 


Val Asp Ala Ser Asp Phe Asp Asp Glu Arg Asn Gly Trp Pro Val Glu 
                165                 170                 175     


Gln Val Trp Lys Glu Met His Lys Leu Leu Pro Phe Ser Pro Asp Ser 
            180                 185                 190         


Val Val Thr His Gly Asp Phe Ser Leu Asp Asn Leu Ile Phe Asp Glu 
        195                 200                 205             


Gly Lys Leu Ile Gly Cys Ile Asp Val Gly Arg Val Gly Ile Ala Asp 
    210                 215                 220                 


Arg Tyr Gln Asp Leu Ala Ile Leu Trp Asn Cys Leu Gly Glu Phe Ser 
225                 230                 235                 240 


Pro Ser Leu Gln Lys Arg Leu Phe Gln Lys Tyr Gly Ile Asp Asn Pro 
                245                 250                 255     


Asp Met Asn Lys Leu Gln Phe His Leu Met Leu Asp Glu Phe Phe 
            260                 265                 270     


<210>  52
<211>  589
<212>  DNA
<213>  synthetic


<220>
<221>  rep_origin
<222>  (1)..(589)
<223>  pBR322_ori

<400>  52
ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc       60

agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt      120

cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt      180

caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc      240

tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa      300

ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac      360

ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg      420

gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga      480

gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact      540

tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaa                  589


<210>  53
<211>  143
<212>  DNA
<213>  symthetic


<220>
<221>  misc_feature
<222>  (1)..(143)
<223>  bom

<400>  53
cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc       60

actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc      120

tacgtgactg ggtcatggct gcg                                              143


<210>  54
<211>  192
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(192)
<223>  rop

<400>  54
gtgaccaaac aggaaaaaac cgcccttaac atggcccgct ttatcagaag ccagacatta       60

acgcttctgg agaaactcaa cgagctggac gcggatgaac aggcagacat ctgtgaatcg      120

cttcacgacc acgctgatga gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt      180

gaaaacctct ga                                                          192


<210>  55
<211>  78
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(78)
<223>  lacI promoter

<400>  55
gacaccatcg aatggcgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt       60

caattcaggg tggtgaat                                                     78


<210>  56
<211>  1083
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(1083)
<223>  lacI encoding lac repressor

<400>  56
gtg aaa cca gta acg tta tac gat gtc gca gag tat gcc ggt gtc tct         48
Val Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser           
1               5                   10                  15                

tat cag acc gtt tcc cgc gtg gtg aac cag gcc agc cac gtt tct gcg         96
Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala           
            20                  25                  30                    

aaa acg cgg gaa aaa gtg gaa gcg gcg atg gcg gag ctg aat tac att        144
Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile           
        35                  40                  45                        

ccc aac cgc gtg gca caa caa ctg gcg ggc aaa cag tcg ttg ctg att        192
Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile           
    50                  55                  60                            

ggc gtt gcc acc tcc agt ctg gcc ctg cac gcg ccg tcg caa att gtc        240
Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val           
65                  70                  75                  80            

gcg gcg att aaa tct cgc gcc gat caa ctg ggt gcc agc gtg gtg gtg        288
Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val           
                85                  90                  95                

tcg atg gta gaa cga agc ggc gtc gaa gcc tgt aaa gcg gcg gtg cac        336
Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His           
            100                 105                 110                   

aat ctt ctc gcg caa cgc gtc agt ggg ctg atc att aac tat ccg ctg        384
Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu           
        115                 120                 125                       

gat gac cag gat gcc att gct gtg gaa gct gcc tgc act aat gtt ccg        432
Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro           
    130                 135                 140                           

gcg tta ttt ctt gat gtc tct gac cag aca ccc atc aac agt att att        480
Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile           
145                 150                 155                 160           

ttc tcc cat gaa gac ggt acg cga ctg ggc gtg gag cat ctg gtc gca        528
Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala           
                165                 170                 175               

ttg ggt cac cag caa atc gcg ctg tta gcg ggc cca tta agt tct gtc        576
Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val           
            180                 185                 190                   

tcg gcg cgt ctg cgt ctg gct ggc tgg cat aaa tat ctc act cgc aat        624
Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn           
        195                 200                 205                       

caa att cag ccg ata gcg gaa cgg gaa ggc gac tgg agt gcc atg tcc        672
Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser           
    210                 215                 220                           

ggt ttt caa caa acc atg caa atg ctg aat gag ggc atc gtt ccc act        720
Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr           
225                 230                 235                 240           

gcg atg ctg gtt gcc aac gat cag atg gcg ctg ggc gca atg cgc gcc        768
Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala           
                245                 250                 255               

att acc gag tcc ggg ctg cgc gtt ggt gcg gat atc tcg gta gtg gga        816
Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly           
            260                 265                 270                   

tac gac gat acc gaa gac agc tca tgt tat atc ccg ccg tta acc acc        864
Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Leu Thr Thr           
        275                 280                 285                       

atc aaa cag gat ttt cgc ctg ctg ggg caa acc agc gtg gac cgc ttg        912
Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu           
    290                 295                 300                           

ctg caa ctc tct cag ggc cag gcg gtg aag ggc aat cag ctg ttg ccc        960
Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro           
305                 310                 315                 320           

gtc tca ctg gtg aaa aga aaa acc acc ctg gcg ccc aat acg caa acc       1008
Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr           
                325                 330                 335               

gcc tct ccc cgc gcg ttg gcc gat tca tta atg cag ctg gca cga cag       1056
Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln           
            340                 345                 350                   

gtt tcc cga ctg gaa agc ggg cag tga                                   1083
Val Ser Arg Leu Glu Ser Gly Gln                                           
        355                 360                                           


<210>  57
<211>  360
<212>  PRT
<213>  synthetic

<400>  57

Val Lys Pro Val Thr Leu Tyr Asp Val Ala Glu Tyr Ala Gly Val Ser 
1               5                   10                  15      


Tyr Gln Thr Val Ser Arg Val Val Asn Gln Ala Ser His Val Ser Ala 
            20                  25                  30          


Lys Thr Arg Glu Lys Val Glu Ala Ala Met Ala Glu Leu Asn Tyr Ile 
        35                  40                  45              


Pro Asn Arg Val Ala Gln Gln Leu Ala Gly Lys Gln Ser Leu Leu Ile 
    50                  55                  60                  


Gly Val Ala Thr Ser Ser Leu Ala Leu His Ala Pro Ser Gln Ile Val 
65                  70                  75                  80  


Ala Ala Ile Lys Ser Arg Ala Asp Gln Leu Gly Ala Ser Val Val Val 
                85                  90                  95      


Ser Met Val Glu Arg Ser Gly Val Glu Ala Cys Lys Ala Ala Val His 
            100                 105                 110         


Asn Leu Leu Ala Gln Arg Val Ser Gly Leu Ile Ile Asn Tyr Pro Leu 
        115                 120                 125             


Asp Asp Gln Asp Ala Ile Ala Val Glu Ala Ala Cys Thr Asn Val Pro 
    130                 135                 140                 


Ala Leu Phe Leu Asp Val Ser Asp Gln Thr Pro Ile Asn Ser Ile Ile 
145                 150                 155                 160 


Phe Ser His Glu Asp Gly Thr Arg Leu Gly Val Glu His Leu Val Ala 
                165                 170                 175     


Leu Gly His Gln Gln Ile Ala Leu Leu Ala Gly Pro Leu Ser Ser Val 
            180                 185                 190         


Ser Ala Arg Leu Arg Leu Ala Gly Trp His Lys Tyr Leu Thr Arg Asn 
        195                 200                 205             


Gln Ile Gln Pro Ile Ala Glu Arg Glu Gly Asp Trp Ser Ala Met Ser 
    210                 215                 220                 


Gly Phe Gln Gln Thr Met Gln Met Leu Asn Glu Gly Ile Val Pro Thr 
225                 230                 235                 240 


Ala Met Leu Val Ala Asn Asp Gln Met Ala Leu Gly Ala Met Arg Ala 
                245                 250                 255     


Ile Thr Glu Ser Gly Leu Arg Val Gly Ala Asp Ile Ser Val Val Gly 
            260                 265                 270         


Tyr Asp Asp Thr Glu Asp Ser Ser Cys Tyr Ile Pro Pro Leu Thr Thr 
        275                 280                 285             


Ile Lys Gln Asp Phe Arg Leu Leu Gly Gln Thr Ser Val Asp Arg Leu 
    290                 295                 300                 


Leu Gln Leu Ser Gln Gly Gln Ala Val Lys Gly Asn Gln Leu Leu Pro 
305                 310                 315                 320 


Val Ser Leu Val Lys Arg Lys Thr Thr Leu Ala Pro Asn Thr Gln Thr 
                325                 330                 335     


Ala Ser Pro Arg Ala Leu Ala Asp Ser Leu Met Gln Leu Ala Arg Gln 
            340                 345                 350         


Val Ser Arg Leu Glu Ser Gly Gln 
        355                 360 


<210>  58
<211>  7772
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(7772)
<223>  Plasmid pP450

<400>  58
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag actcgaccat ggaattatca caagtttgta caaaaaagga      120

ggctggcgcc ggaaccaatt cagtcgactg gatccaagaa ggagatataa ccccctcagc      180

agcgatggcg acaatggagg tagaggccgc ggccgccacg gtgctggccg cgcccttgct      240

gtcctcctcc gcgatcctca aactgctgct attcgtagtg acgctctcgt acctggcccg      300

agccctgagg cggccacgca aaagcaccac caagtgcagc agcacaacgt gcgcctcgcc      360

cccggccggc gttggcaacc cgccgctccc accgggtccc gtgccgtggc ccgtcgtcgg      420

caacctgccg gagatgctgc tgaacaagcc ggcattccgc tggatccacc agatgatgcg      480

cgagatgggc acggacatcg cctgcgtcaa gcttggcggc gtccacgtcg tgtccatcac      540

ctgcccggag atcgcgcggg aggtgctccg gaagcaggac gccaacttca tatcccgccc      600

gctcaccttc gcctccgaga cgttcagcgg cgggtaccgg aacgccgtgc tctcgcccta      660

cggcgaccag tggaagaaga tgcgccgcgt cctcacctcc gagatcatct gcccgtcccg      720

ccacgcctgg ctccacgaca agcgcaccga cgaggccgac aacctcaccc gctacgtcta      780

caacctcgcc accaaagccg ccaccggcga cgtcgccgtc gacgtcaggc acgtcgctcg      840

tcactattgc ggcaacgtta tccgccgcct catgttcaac aggcgctact tcggcgagcc      900

ccaggctgac ggcggtccgg ggccgatgga ggtgctgcat atggacgccg tgttcacctc      960

cctcggcctc ctctacgcct tctgcgtctc cgactacctc ccctggctgc ggggcctcga     1020

cctcgacggc cacgagaaga tcgtcaagga ggctaacgtg gcggtgaaca ggctccacga     1080

cacggtcatc gacgaccggt ggaggcagtg gaagagcggc gagcggcagg agatggagga     1140

cttcctggat gtgctcatca ctctcaagga cgcccagggc aacccgctgc tgaccatcga     1200

ggaggtcaaa gcgcagtcac aggacatcac gttcgcggcg gtggacaacc cgtcgaacgc     1260

cgtggagtgg gcgctggcag agatggtgaa caacccggag gtgatggcga aggcgatgga     1320

ggagctggac cgcgtcgtcg gacgggagag gctagtgcag gagtcggaca ttccgaagct     1380

caactacgtg aaggcctgca tccgggaggc tttccgtctg cacccggtgg cgcccttcaa     1440

cgtgccccac gtcgcgctcg ccgacaccac catcgccggc taccgcgttc ccaagggcag     1500

ccacgtgatc ctgagccgca cggggctggg ccgcaacccg cgcgtgtggg acgagcccct     1560

gcgcttctac ccggaccgac acctcgccac cgccgcgtcc gacgtcgcgc tcaccgagaa     1620

cgacctgcgg ttcatctcct tcagcaccgg ccgccgcggc tgcatcgccg cgtcgctcgg     1680

caccgccatg agcgtcatgc tcttcggaag gctcctgcag gggttcacct ggagcaagcc     1740

cgccggggtg gaggccgtgg acctcagcga gtccaagagc gacaccttca tggccacccc     1800

gctggtgctg cacgctgagc ccaggctgcc ggcgcacctc tacccgtcca tctccatcgg     1860

atcgccagct gagggagaaa acctgtactt ccagggtcaa ttcagcaaag gagaagaact     1920

tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt     1980

ttctgtcagt ggagagggtg aaggtgatgc tacatacgga aaactcaccc ttaaatttat     2040

ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactc tgacctatgg     2100

tgttcaatgc ttttcccgtt atccggatca catgaaacgg catgactttt tcaagagtgc     2160

catgcccgaa ggttatgtac aggaacgcac tatatctttc aaagatgacg ggaactacaa     2220

gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt aatcgtatcg agttaaaggg     2280

tattgatttt aaagaagatg gaaacattct cggacacaaa ctagagtaca actataactc     2340

acacaatgta tacatcacgg cagacaaaca aaagaatgga atcaaagcta acttcaaaat     2400

tcgccacaac attgaagatg gttccgttca actagcagac cattatcaac aaaatactcc     2460

aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcgacac aatctgccct     2520

ttcgaaagat cccaacgaaa agcgtgacca catggtcctt cttgagtttg taactgctgc     2580

tgggattaca catggcatgg atgagctcta caaaaagctt gcggcccatc atcatcacca     2640

ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc     2700

caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt     2760

tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg tagcggcgca     2820

ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta     2880

gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt     2940

caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac     3000

cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt     3060

tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga     3120

acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg     3180

gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata     3240

ttaacgttta caatttcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt     3300

ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt agaaaaactc     3360

atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg     3420

aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag     3480

atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc     3540

ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga     3600

gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc cattacgctc     3660

gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag     3720

acgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg     3780

caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac     3840

ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg     3900

gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat     3960

ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc     4020

atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc     4080

ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc tagagcaaga     4140

cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag     4200

ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc     4260

ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct     4320

tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa     4380

ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag     4440

tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc     4500

tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg     4560

actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca     4620

cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat     4680

gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg     4740

tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc     4800

ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc     4860

ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc     4920

cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg     4980

cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga     5040

gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt     5100

cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca     5160

gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca     5220

cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg     5280

accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg     5340

cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca     5400

tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg     5460

gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt     5520

ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact     5580

gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg     5640

cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta     5700

ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag     5760

ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt     5820

gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt     5880

gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg     5940

agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg     6000

aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat     6060

accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg     6120

acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt     6180

gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc     6240

aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg     6300

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc     6360

caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag     6420

tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg     6480

gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat     6540

ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg     6600

cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag     6660

catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat     6720

ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt     6780

atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc     6840

gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg     6900

ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac     6960

attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat     7020

cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc     7080

gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat     7140

cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag     7200

caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc     7260

catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac     7320

gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg     7380

tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa     7440

ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca     7500

ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg     7560

catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc     7620

cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg     7680

cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg     7740

cgtagaggat cgagatctcg atcccgcgaa at                                   7772


<210>  59
<211>  2469
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(2469)
<223>  Membrane protein P450(SbCYP79A1)-GFP-His8

<400>  59
atg gcg aca atg gag gta gag gcc gcg gcc gcc acg gtg ctg gcc gcg         48
Met Ala Thr Met Glu Val Glu Ala Ala Ala Ala Thr Val Leu Ala Ala           
1               5                   10                  15                

ccc ttg ctg tcc tcc tcc gcg atc ctc aaa ctg ctg cta ttc gta gtg         96
Pro Leu Leu Ser Ser Ser Ala Ile Leu Lys Leu Leu Leu Phe Val Val           
            20                  25                  30                    

acg ctc tcg tac ctg gcc cga gcc ctg agg cgg cca cgc aaa agc acc        144
Thr Leu Ser Tyr Leu Ala Arg Ala Leu Arg Arg Pro Arg Lys Ser Thr           
        35                  40                  45                        

acc aag tgc agc agc aca acg tgc gcc tcg ccc ccg gcc ggc gtt ggc        192
Thr Lys Cys Ser Ser Thr Thr Cys Ala Ser Pro Pro Ala Gly Val Gly           
    50                  55                  60                            

aac ccg ccg ctc cca ccg ggt ccc gtg ccg tgg ccc gtc gtc ggc aac        240
Asn Pro Pro Leu Pro Pro Gly Pro Val Pro Trp Pro Val Val Gly Asn           
65                  70                  75                  80            

ctg ccg gag atg ctg ctg aac aag ccg gca ttc cgc tgg atc cac cag        288
Leu Pro Glu Met Leu Leu Asn Lys Pro Ala Phe Arg Trp Ile His Gln           
                85                  90                  95                

atg atg cgc gag atg ggc acg gac atc gcc tgc gtc aag ctt ggc ggc        336
Met Met Arg Glu Met Gly Thr Asp Ile Ala Cys Val Lys Leu Gly Gly           
            100                 105                 110                   

gtc cac gtc gtg tcc atc acc tgc ccg gag atc gcg cgg gag gtg ctc        384
Val His Val Val Ser Ile Thr Cys Pro Glu Ile Ala Arg Glu Val Leu           
        115                 120                 125                       

cgg aag cag gac gcc aac ttc ata tcc cgc ccg ctc acc ttc gcc tcc        432
Arg Lys Gln Asp Ala Asn Phe Ile Ser Arg Pro Leu Thr Phe Ala Ser           
    130                 135                 140                           

gag acg ttc agc ggc ggg tac cgg aac gcc gtg ctc tcg ccc tac ggc        480
Glu Thr Phe Ser Gly Gly Tyr Arg Asn Ala Val Leu Ser Pro Tyr Gly           
145                 150                 155                 160           

gac cag tgg aag aag atg cgc cgc gtc ctc acc tcc gag atc atc tgc        528
Asp Gln Trp Lys Lys Met Arg Arg Val Leu Thr Ser Glu Ile Ile Cys           
                165                 170                 175               

ccg tcc cgc cac gcc tgg ctc cac gac aag cgc acc gac gag gcc gac        576
Pro Ser Arg His Ala Trp Leu His Asp Lys Arg Thr Asp Glu Ala Asp           
            180                 185                 190                   

aac ctc acc cgc tac gtc tac aac ctc gcc acc aaa gcc gcc acc ggc        624
Asn Leu Thr Arg Tyr Val Tyr Asn Leu Ala Thr Lys Ala Ala Thr Gly           
        195                 200                 205                       

gac gtc gcc gtc gac gtc agg cac gtc gct cgt cac tat tgc ggc aac        672
Asp Val Ala Val Asp Val Arg His Val Ala Arg His Tyr Cys Gly Asn           
    210                 215                 220                           

gtt atc cgc cgc ctc atg ttc aac agg cgc tac ttc ggc gag ccc cag        720
Val Ile Arg Arg Leu Met Phe Asn Arg Arg Tyr Phe Gly Glu Pro Gln           
225                 230                 235                 240           

gct gac ggc ggt ccg ggg ccg atg gag gtg ctg cat atg gac gcc gtg        768
Ala Asp Gly Gly Pro Gly Pro Met Glu Val Leu His Met Asp Ala Val           
                245                 250                 255               

ttc acc tcc ctc ggc ctc ctc tac gcc ttc tgc gtc tcc gac tac ctc        816
Phe Thr Ser Leu Gly Leu Leu Tyr Ala Phe Cys Val Ser Asp Tyr Leu           
            260                 265                 270                   

ccc tgg ctg cgg ggc ctc gac ctc gac ggc cac gag aag atc gtc aag        864
Pro Trp Leu Arg Gly Leu Asp Leu Asp Gly His Glu Lys Ile Val Lys           
        275                 280                 285                       

gag gct aac gtg gcg gtg aac agg ctc cac gac acg gtc atc gac gac        912
Glu Ala Asn Val Ala Val Asn Arg Leu His Asp Thr Val Ile Asp Asp           
    290                 295                 300                           

cgg tgg agg cag tgg aag agc ggc gag cgg cag gag atg gag gac ttc        960
Arg Trp Arg Gln Trp Lys Ser Gly Glu Arg Gln Glu Met Glu Asp Phe           
305                 310                 315                 320           

ctg gat gtg ctc atc act ctc aag gac gcc cag ggc aac ccg ctg ctg       1008
Leu Asp Val Leu Ile Thr Leu Lys Asp Ala Gln Gly Asn Pro Leu Leu           
                325                 330                 335               

acc atc gag gag gtc aaa gcg cag tca cag gac atc acg ttc gcg gcg       1056
Thr Ile Glu Glu Val Lys Ala Gln Ser Gln Asp Ile Thr Phe Ala Ala           
            340                 345                 350                   

gtg gac aac ccg tcg aac gcc gtg gag tgg gcg ctg gca gag atg gtg       1104
Val Asp Asn Pro Ser Asn Ala Val Glu Trp Ala Leu Ala Glu Met Val           
        355                 360                 365                       

aac aac ccg gag gtg atg gcg aag gcg atg gag gag ctg gac cgc gtc       1152
Asn Asn Pro Glu Val Met Ala Lys Ala Met Glu Glu Leu Asp Arg Val           
    370                 375                 380                           

gtc gga cgg gag agg cta gtg cag gag tcg gac att ccg aag ctc aac       1200
Val Gly Arg Glu Arg Leu Val Gln Glu Ser Asp Ile Pro Lys Leu Asn           
385                 390                 395                 400           

tac gtg aag gcc tgc atc cgg gag gct ttc cgt ctg cac ccg gtg gcg       1248
Tyr Val Lys Ala Cys Ile Arg Glu Ala Phe Arg Leu His Pro Val Ala           
                405                 410                 415               

ccc ttc aac gtg ccc cac gtc gcg ctc gcc gac acc acc atc gcc ggc       1296
Pro Phe Asn Val Pro His Val Ala Leu Ala Asp Thr Thr Ile Ala Gly           
            420                 425                 430                   

tac cgc gtt ccc aag ggc agc cac gtg atc ctg agc cgc acg ggg ctg       1344
Tyr Arg Val Pro Lys Gly Ser His Val Ile Leu Ser Arg Thr Gly Leu           
        435                 440                 445                       

ggc cgc aac ccg cgc gtg tgg gac gag ccc ctg cgc ttc tac ccg gac       1392
Gly Arg Asn Pro Arg Val Trp Asp Glu Pro Leu Arg Phe Tyr Pro Asp           
    450                 455                 460                           

cga cac ctc gcc acc gcc gcg tcc gac gtc gcg ctc acc gag aac gac       1440
Arg His Leu Ala Thr Ala Ala Ser Asp Val Ala Leu Thr Glu Asn Asp           
465                 470                 475                 480           

ctg cgg ttc atc tcc ttc agc acc ggc cgc cgc ggc tgc atc gcc gcg       1488
Leu Arg Phe Ile Ser Phe Ser Thr Gly Arg Arg Gly Cys Ile Ala Ala           
                485                 490                 495               

tcg ctc ggc acc gcc atg agc gtc atg ctc ttc gga agg ctc ctg cag       1536
Ser Leu Gly Thr Ala Met Ser Val Met Leu Phe Gly Arg Leu Leu Gln           
            500                 505                 510                   

ggg ttc acc tgg agc aag ccc gcc ggg gtg gag gcc gtg gac ctc agc       1584
Gly Phe Thr Trp Ser Lys Pro Ala Gly Val Glu Ala Val Asp Leu Ser           
        515                 520                 525                       

gag tcc aag agc gac acc ttc atg gcc acc ccg ctg gtg ctg cac gct       1632
Glu Ser Lys Ser Asp Thr Phe Met Ala Thr Pro Leu Val Leu His Ala           
    530                 535                 540                           

gag ccc agg ctg ccg gcg cac ctc tac ccg tcc atc tcc atc gga tcg       1680
Glu Pro Arg Leu Pro Ala His Leu Tyr Pro Ser Ile Ser Ile Gly Ser           
545                 550                 555                 560           

cca gct gag gga gaa aac ctg tac ttc cag ggt caa ttc agc aaa gga       1728
Pro Ala Glu Gly Glu Asn Leu Tyr Phe Gln Gly Gln Phe Ser Lys Gly           
                565                 570                 575               

gaa gaa ctt ttc act gga gtt gtc cca att ctt gtt gaa tta gat ggt       1776
Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly           
            580                 585                 590                   

gat gtt aat ggg cac aaa ttt tct gtc agt gga gag ggt gaa ggt gat       1824
Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp           
        595                 600                 605                       

gct aca tac gga aaa ctc acc ctt aaa ttt att tgc act act gga aaa       1872
Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys           
    610                 615                 620                           

cta cct gtt cca tgg cca aca ctt gtc act act ctg acc tat ggt gtt       1920
Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val           
625                 630                 635                 640           

caa tgc ttt tcc cgt tat ccg gat cac atg aaa cgg cat gac ttt ttc       1968
Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe           
                645                 650                 655               

aag agt gcc atg ccc gaa ggt tat gta cag gaa cgc act ata tct ttc       2016
Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe           
            660                 665                 670                   

aaa gat gac ggg aac tac aag acg cgt gct gaa gtc aag ttt gaa ggt       2064
Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly           
        675                 680                 685                       

gat acc ctt gtt aat cgt atc gag tta aag ggt att gat ttt aaa gaa       2112
Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu           
    690                 695                 700                           

gat gga aac att ctc gga cac aaa cta gag tac aac tat aac tca cac       2160
Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His           
705                 710                 715                 720           

aat gta tac atc acg gca gac aaa caa aag aat gga atc aaa gct aac       2208
Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn           
                725                 730                 735               

ttc aaa att cgc cac aac att gaa gat ggt tcc gtt caa cta gca gac       2256
Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp           
            740                 745                 750                   

cat tat caa caa aat act cca att ggc gat ggc cct gtc ctt tta cca       2304
His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro           
        755                 760                 765                       

gac aac cat tac ctg tcg aca caa tct gcc ctt tcg aaa gat ccc aac       2352
Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn           
    770                 775                 780                           

gaa aag cgt gac cac atg gtc ctt ctt gag ttt gta act gct gct ggg       2400
Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly           
785                 790                 795                 800           

att aca cat ggc atg gat gag ctc tac aaa aag ctt gcg gcc cat cat       2448
Ile Thr His Gly Met Asp Glu Leu Tyr Lys Lys Leu Ala Ala His His           
                805                 810                 815               

cat cac cac cac cac cac tga                                           2469
His His His His His His                                                   
            820                                                           


<210>  60
<211>  822
<212>  PRT
<213>  synthetic

<400>  60

Met Ala Thr Met Glu Val Glu Ala Ala Ala Ala Thr Val Leu Ala Ala 
1               5                   10                  15      


Pro Leu Leu Ser Ser Ser Ala Ile Leu Lys Leu Leu Leu Phe Val Val 
            20                  25                  30          


Thr Leu Ser Tyr Leu Ala Arg Ala Leu Arg Arg Pro Arg Lys Ser Thr 
        35                  40                  45              


Thr Lys Cys Ser Ser Thr Thr Cys Ala Ser Pro Pro Ala Gly Val Gly 
    50                  55                  60                  


Asn Pro Pro Leu Pro Pro Gly Pro Val Pro Trp Pro Val Val Gly Asn 
65                  70                  75                  80  


Leu Pro Glu Met Leu Leu Asn Lys Pro Ala Phe Arg Trp Ile His Gln 
                85                  90                  95      


Met Met Arg Glu Met Gly Thr Asp Ile Ala Cys Val Lys Leu Gly Gly 
            100                 105                 110         


Val His Val Val Ser Ile Thr Cys Pro Glu Ile Ala Arg Glu Val Leu 
        115                 120                 125             


Arg Lys Gln Asp Ala Asn Phe Ile Ser Arg Pro Leu Thr Phe Ala Ser 
    130                 135                 140                 


Glu Thr Phe Ser Gly Gly Tyr Arg Asn Ala Val Leu Ser Pro Tyr Gly 
145                 150                 155                 160 


Asp Gln Trp Lys Lys Met Arg Arg Val Leu Thr Ser Glu Ile Ile Cys 
                165                 170                 175     


Pro Ser Arg His Ala Trp Leu His Asp Lys Arg Thr Asp Glu Ala Asp 
            180                 185                 190         


Asn Leu Thr Arg Tyr Val Tyr Asn Leu Ala Thr Lys Ala Ala Thr Gly 
        195                 200                 205             


Asp Val Ala Val Asp Val Arg His Val Ala Arg His Tyr Cys Gly Asn 
    210                 215                 220                 


Val Ile Arg Arg Leu Met Phe Asn Arg Arg Tyr Phe Gly Glu Pro Gln 
225                 230                 235                 240 


Ala Asp Gly Gly Pro Gly Pro Met Glu Val Leu His Met Asp Ala Val 
                245                 250                 255     


Phe Thr Ser Leu Gly Leu Leu Tyr Ala Phe Cys Val Ser Asp Tyr Leu 
            260                 265                 270         


Pro Trp Leu Arg Gly Leu Asp Leu Asp Gly His Glu Lys Ile Val Lys 
        275                 280                 285             


Glu Ala Asn Val Ala Val Asn Arg Leu His Asp Thr Val Ile Asp Asp 
    290                 295                 300                 


Arg Trp Arg Gln Trp Lys Ser Gly Glu Arg Gln Glu Met Glu Asp Phe 
305                 310                 315                 320 


Leu Asp Val Leu Ile Thr Leu Lys Asp Ala Gln Gly Asn Pro Leu Leu 
                325                 330                 335     


Thr Ile Glu Glu Val Lys Ala Gln Ser Gln Asp Ile Thr Phe Ala Ala 
            340                 345                 350         


Val Asp Asn Pro Ser Asn Ala Val Glu Trp Ala Leu Ala Glu Met Val 
        355                 360                 365             


Asn Asn Pro Glu Val Met Ala Lys Ala Met Glu Glu Leu Asp Arg Val 
    370                 375                 380                 


Val Gly Arg Glu Arg Leu Val Gln Glu Ser Asp Ile Pro Lys Leu Asn 
385                 390                 395                 400 


Tyr Val Lys Ala Cys Ile Arg Glu Ala Phe Arg Leu His Pro Val Ala 
                405                 410                 415     


Pro Phe Asn Val Pro His Val Ala Leu Ala Asp Thr Thr Ile Ala Gly 
            420                 425                 430         


Tyr Arg Val Pro Lys Gly Ser His Val Ile Leu Ser Arg Thr Gly Leu 
        435                 440                 445             


Gly Arg Asn Pro Arg Val Trp Asp Glu Pro Leu Arg Phe Tyr Pro Asp 
    450                 455                 460                 


Arg His Leu Ala Thr Ala Ala Ser Asp Val Ala Leu Thr Glu Asn Asp 
465                 470                 475                 480 


Leu Arg Phe Ile Ser Phe Ser Thr Gly Arg Arg Gly Cys Ile Ala Ala 
                485                 490                 495     


Ser Leu Gly Thr Ala Met Ser Val Met Leu Phe Gly Arg Leu Leu Gln 
            500                 505                 510         


Gly Phe Thr Trp Ser Lys Pro Ala Gly Val Glu Ala Val Asp Leu Ser 
        515                 520                 525             


Glu Ser Lys Ser Asp Thr Phe Met Ala Thr Pro Leu Val Leu His Ala 
    530                 535                 540                 


Glu Pro Arg Leu Pro Ala His Leu Tyr Pro Ser Ile Ser Ile Gly Ser 
545                 550                 555                 560 


Pro Ala Glu Gly Glu Asn Leu Tyr Phe Gln Gly Gln Phe Ser Lys Gly 
                565                 570                 575     


Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp Gly 
            580                 585                 590         


Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly Asp 
        595                 600                 605             


Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys 
    610                 615                 620                 


Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly Val 
625                 630                 635                 640 


Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Arg His Asp Phe Phe 
                645                 650                 655     


Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe 
            660                 665                 670         


Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu Gly 
        675                 680                 685             


Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu 
    690                 695                 700                 


Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Tyr Asn Ser His 
705                 710                 715                 720 


Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn 
                725                 730                 735     


Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser Val Gln Leu Ala Asp 
            740                 745                 750         


His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro 
        755                 760                 765             


Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn 
    770                 775                 780                 


Glu Lys Arg Asp His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly 
785                 790                 795                 800 


Ile Thr His Gly Met Asp Glu Leu Tyr Lys Lys Leu Ala Ala His His 
                805                 810                 815     


His His His His His His 
            820         


<210>  61
<211>  6878
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(6878)
<223>  Plasmid pHtpX

<400>  61
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag accagtttat gatgcgaatc gcgctcttcc tgctaacgaa      120

cctggccgta atggtcgttt tcgggctggt actgagcctg acagggatac agtcgagcag      180

cgttcagggg ctgatgatca tggccttgct gttcggtttt ggtggttcct tcgtttcgct      240

tctgatgtcc aaatggatgg cattacgatc tgttggcggg gaagtgatcg agcaaccgcg      300

taacgaaagg gaacgttggc tggtcaatac tgtagcaacc caggctcgtc aggcggggat      360

cgctatgccg caagtggcta tctaccatgc gccggacatc aacgcttttg caaccggtgc      420

gcgccgtgat gcctctctgg ttgctgtcag caccggtttg ctgcagaaca tgagcccgga      480

tgaagccgag gcggtaattg ctcacgaaat cagccacatc gccaatggtg atatggtcac      540

catgacgctg attcagggcg tggtgaacac cttcgttatc tttatttccc gtattctggc      600

gcagcttgcc gcgggtttta tgggcggaaa tcgtgatgaa ggtgaagaga gcaacggcaa      660

cccgctgatc tactttgcgg ttgcaacggt tctggaactg gtgtttggta ttctggcgag      720

cattatcacc atgtggttct cgcgtcatcg tgaattccat gctgatgccg gttcggcaaa      780

actggttggt cgcgagaaaa tgattgccgc gctgcagcgc ctgaaaacca gctatgaacc      840

gcaagaagca accagcatga tggctctctg cattaacggt aagtcgaaat cgctcagtga      900

gttgttcatg acccacccgc cgctggataa acgaattgaa gctctgcgta cgggtgaata      960

cctgaagtcg gtacctggat ccgaaaacct gtacttccag ggtcaattca gcaaaggaga     1020

agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca     1080

caaattttct gtcagtggag agggtgaagg tgatgctaca tacggaaaac tcacccttaa     1140

atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactctgac     1200

ctatggtgtt caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa     1260

gagtgccatg cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggaa     1320

ctacaagacg cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt     1380

aaagggtatt gattttaaag aagatggaaa cattctcgga cacaaactag agtacaacta     1440

taactcacac aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt     1500

caaaattcgc cacaacattg aagatggttc cgttcaacta gcagaccatt atcaacaaaa     1560

tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc     1620

tgccctttcg aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac     1680

tgctgctggg attacacatg gcatggatga gctctacaaa aagcttgcgg cccatcatca     1740

tcaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc     1800

tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag     1860

gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc     1920

ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc     1980

gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt     2040

ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac     2100

ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag     2160

acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa     2220

actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg     2280

atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac     2340

aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct     2400

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa     2460

aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata     2520

tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat     2580

ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa     2640

tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc     2700

cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt     2760

acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg     2820

agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa     2880

ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc     2940

taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg     3000

agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct     3060

gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc     3120

tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc     3180

gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga     3240

gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc     3300

agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     3360

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     3420

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     3480

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     3540

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     3600

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     3660

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     3720

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     3780

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     3840

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     3900

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     3960

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     4020

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     4080

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     4140

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg     4200

gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt     4260

aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg     4320

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa     4380

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc     4440

gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc     4500

tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata     4560

aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg     4620

ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg     4680

gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta     4740

tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca     4800

gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg     4860

gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt     4920

catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt     4980

atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac     5040

gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc     5100

tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt     5160

ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg     5220

aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc     5280

ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag     5340

gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt     5400

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     5460

atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt     5520

caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag     5580

caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg     5640

cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc     5700

aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc     5760

aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc     5820

ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag     5880

atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa     5940

cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc     6000

ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc     6060

cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt     6120

aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc     6180

gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga     6240

tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc     6300

aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag     6360

ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt     6420

caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt     6480

tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc     6540

gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact     6600

cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga     6660

atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac     6720

ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga     6780

tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc     6840

gtccggcgta gaggatcgag atctcgatcc cgcgaaat                             6878


<210>  62
<211>  1671
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(1671)
<223>  Membrane protein HtpX-GFP-His8

<400>  62
atg atg cga atc gcg ctc ttc ctg cta acg aac ctg gcc gta atg gtc         48
Met Met Arg Ile Ala Leu Phe Leu Leu Thr Asn Leu Ala Val Met Val           
1               5                   10                  15                

gtt ttc ggg ctg gta ctg agc ctg aca ggg ata cag tcg agc agc gtt         96
Val Phe Gly Leu Val Leu Ser Leu Thr Gly Ile Gln Ser Ser Ser Val           
            20                  25                  30                    

cag ggg ctg atg atc atg gcc ttg ctg ttc ggt ttt ggt ggt tcc ttc        144
Gln Gly Leu Met Ile Met Ala Leu Leu Phe Gly Phe Gly Gly Ser Phe           
        35                  40                  45                        

gtt tcg ctt ctg atg tcc aaa tgg atg gca tta cga tct gtt ggc ggg        192
Val Ser Leu Leu Met Ser Lys Trp Met Ala Leu Arg Ser Val Gly Gly           
    50                  55                  60                            

gaa gtg atc gag caa ccg cgt aac gaa agg gaa cgt tgg ctg gtc aat        240
Glu Val Ile Glu Gln Pro Arg Asn Glu Arg Glu Arg Trp Leu Val Asn           
65                  70                  75                  80            

act gta gca acc cag gct cgt cag gcg ggg atc gct atg ccg caa gtg        288
Thr Val Ala Thr Gln Ala Arg Gln Ala Gly Ile Ala Met Pro Gln Val           
                85                  90                  95                

gct atc tac cat gcg ccg gac atc aac gct ttt gca acc ggt gcg cgc        336
Ala Ile Tyr His Ala Pro Asp Ile Asn Ala Phe Ala Thr Gly Ala Arg           
            100                 105                 110                   

cgt gat gcc tct ctg gtt gct gtc agc acc ggt ttg ctg cag aac atg        384
Arg Asp Ala Ser Leu Val Ala Val Ser Thr Gly Leu Leu Gln Asn Met           
        115                 120                 125                       

agc ccg gat gaa gcc gag gcg gta att gct cac gaa atc agc cac atc        432
Ser Pro Asp Glu Ala Glu Ala Val Ile Ala His Glu Ile Ser His Ile           
    130                 135                 140                           

gcc aat ggt gat atg gtc acc atg acg ctg att cag ggc gtg gtg aac        480
Ala Asn Gly Asp Met Val Thr Met Thr Leu Ile Gln Gly Val Val Asn           
145                 150                 155                 160           

acc ttc gtt atc ttt att tcc cgt att ctg gcg cag ctt gcc gcg ggt        528
Thr Phe Val Ile Phe Ile Ser Arg Ile Leu Ala Gln Leu Ala Ala Gly           
                165                 170                 175               

ttt atg ggc gga aat cgt gat gaa ggt gaa gag agc aac ggc aac ccg        576
Phe Met Gly Gly Asn Arg Asp Glu Gly Glu Glu Ser Asn Gly Asn Pro           
            180                 185                 190                   

ctg atc tac ttt gcg gtt gca acg gtt ctg gaa ctg gtg ttt ggt att        624
Leu Ile Tyr Phe Ala Val Ala Thr Val Leu Glu Leu Val Phe Gly Ile           
        195                 200                 205                       

ctg gcg agc att atc acc atg tgg ttc tcg cgt cat cgt gaa ttc cat        672
Leu Ala Ser Ile Ile Thr Met Trp Phe Ser Arg His Arg Glu Phe His           
    210                 215                 220                           

gct gat gcc ggt tcg gca aaa ctg gtt ggt cgc gag aaa atg att gcc        720
Ala Asp Ala Gly Ser Ala Lys Leu Val Gly Arg Glu Lys Met Ile Ala           
225                 230                 235                 240           

gcg ctg cag cgc ctg aaa acc agc tat gaa ccg caa gaa gca acc agc        768
Ala Leu Gln Arg Leu Lys Thr Ser Tyr Glu Pro Gln Glu Ala Thr Ser           
                245                 250                 255               

atg atg gct ctc tgc att aac ggt aag tcg aaa tcg ctc agt gag ttg        816
Met Met Ala Leu Cys Ile Asn Gly Lys Ser Lys Ser Leu Ser Glu Leu           
            260                 265                 270                   

ttc atg acc cac ccg ccg ctg gat aaa cga att gaa gct ctg cgt acg        864
Phe Met Thr His Pro Pro Leu Asp Lys Arg Ile Glu Ala Leu Arg Thr           
        275                 280                 285                       

ggt gaa tac ctg aag tcg gta cct gga tcc gaa aac ctg tac ttc cag        912
Gly Glu Tyr Leu Lys Ser Val Pro Gly Ser Glu Asn Leu Tyr Phe Gln           
    290                 295                 300                           

ggt caa ttc agc aaa gga gaa gaa ctt ttc act gga gtt gtc cca att        960
Gly Gln Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile           
305                 310                 315                 320           

ctt gtt gaa tta gat ggt gat gtt aat ggg cac aaa ttt tct gtc agt       1008
Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser           
                325                 330                 335               

gga gag ggt gaa ggt gat gct aca tac gga aaa ctc acc ctt aaa ttt       1056
Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe           
            340                 345                 350                   

att tgc act act gga aaa cta cct gtt cca tgg cca aca ctt gtc act       1104
Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr           
        355                 360                 365                       

act ctg acc tat ggt gtt caa tgc ttt tcc cgt tat ccg gat cac atg       1152
Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met           
    370                 375                 380                           

aaa cgg cat gac ttt ttc aag agt gcc atg ccc gaa ggt tat gta cag       1200
Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln           
385                 390                 395                 400           

gaa cgc act ata tct ttc aaa gat gac ggg aac tac aag acg cgt gct       1248
Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala           
                405                 410                 415               

gaa gtc aag ttt gaa ggt gat acc ctt gtt aat cgt atc gag tta aag       1296
Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys           
            420                 425                 430                   

ggt att gat ttt aaa gaa gat gga aac att ctc gga cac aaa cta gag       1344
Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu           
        435                 440                 445                       

tac aac tat aac tca cac aat gta tac atc acg gca gac aaa caa aag       1392
Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys           
    450                 455                 460                           

aat gga atc aaa gct aac ttc aaa att cgc cac aac att gaa gat ggt       1440
Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly           
465                 470                 475                 480           

tcc gtt caa cta gca gac cat tat caa caa aat act cca att ggc gat       1488
Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp           
                485                 490                 495               

ggc cct gtc ctt tta cca gac aac cat tac ctg tcg aca caa tct gcc       1536
Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala           
            500                 505                 510                   

ctt tcg aaa gat ccc aac gaa aag cgt gac cac atg gtc ctt ctt gag       1584
Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu           
        515                 520                 525                       

ttt gta act gct gct ggg att aca cat ggc atg gat gag ctc tac aaa       1632
Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys           
    530                 535                 540                           

aag ctt gcg gcc cat cat cat cac cac cac cac cac tga                   1671
Lys Leu Ala Ala His His His His His His His His                           
545                 550                 555                               


<210>  63
<211>  556
<212>  PRT
<213>  synthetic

<400>  63

Met Met Arg Ile Ala Leu Phe Leu Leu Thr Asn Leu Ala Val Met Val 
1               5                   10                  15      


Val Phe Gly Leu Val Leu Ser Leu Thr Gly Ile Gln Ser Ser Ser Val 
            20                  25                  30          


Gln Gly Leu Met Ile Met Ala Leu Leu Phe Gly Phe Gly Gly Ser Phe 
        35                  40                  45              


Val Ser Leu Leu Met Ser Lys Trp Met Ala Leu Arg Ser Val Gly Gly 
    50                  55                  60                  


Glu Val Ile Glu Gln Pro Arg Asn Glu Arg Glu Arg Trp Leu Val Asn 
65                  70                  75                  80  


Thr Val Ala Thr Gln Ala Arg Gln Ala Gly Ile Ala Met Pro Gln Val 
                85                  90                  95      


Ala Ile Tyr His Ala Pro Asp Ile Asn Ala Phe Ala Thr Gly Ala Arg 
            100                 105                 110         


Arg Asp Ala Ser Leu Val Ala Val Ser Thr Gly Leu Leu Gln Asn Met 
        115                 120                 125             


Ser Pro Asp Glu Ala Glu Ala Val Ile Ala His Glu Ile Ser His Ile 
    130                 135                 140                 


Ala Asn Gly Asp Met Val Thr Met Thr Leu Ile Gln Gly Val Val Asn 
145                 150                 155                 160 


Thr Phe Val Ile Phe Ile Ser Arg Ile Leu Ala Gln Leu Ala Ala Gly 
                165                 170                 175     


Phe Met Gly Gly Asn Arg Asp Glu Gly Glu Glu Ser Asn Gly Asn Pro 
            180                 185                 190         


Leu Ile Tyr Phe Ala Val Ala Thr Val Leu Glu Leu Val Phe Gly Ile 
        195                 200                 205             


Leu Ala Ser Ile Ile Thr Met Trp Phe Ser Arg His Arg Glu Phe His 
    210                 215                 220                 


Ala Asp Ala Gly Ser Ala Lys Leu Val Gly Arg Glu Lys Met Ile Ala 
225                 230                 235                 240 


Ala Leu Gln Arg Leu Lys Thr Ser Tyr Glu Pro Gln Glu Ala Thr Ser 
                245                 250                 255     


Met Met Ala Leu Cys Ile Asn Gly Lys Ser Lys Ser Leu Ser Glu Leu 
            260                 265                 270         


Phe Met Thr His Pro Pro Leu Asp Lys Arg Ile Glu Ala Leu Arg Thr 
        275                 280                 285             


Gly Glu Tyr Leu Lys Ser Val Pro Gly Ser Glu Asn Leu Tyr Phe Gln 
    290                 295                 300                 


Gly Gln Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile 
305                 310                 315                 320 


Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser 
                325                 330                 335     


Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe 
            340                 345                 350         


Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr 
        355                 360                 365             


Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met 
    370                 375                 380                 


Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln 
385                 390                 395                 400 


Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala 
                405                 410                 415     


Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys 
            420                 425                 430         


Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu 
        435                 440                 445             


Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp Lys Gln Lys 
    450                 455                 460                 


Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly 
465                 470                 475                 480 


Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp 
                485                 490                 495     


Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala 
            500                 505                 510         


Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu 
        515                 520                 525             


Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys 
    530                 535                 540                 


Lys Leu Ala Ala His His His His His His His His 
545                 550                 555     


<210>  64
<211>  7658
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(7658)
<223>  Plasmid pYqiK

<400>  64
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag accagtttat ggatgatatt gttaattctg tgccctcctg      120

gatgtttacc gcaattattg ccgtatgcat tctgtttatt attggaatta ttttcgccag      180

gctctatcgt cgcgcttcgg cagagcaagc ttttgttcgt actggtttag gtgggcaaaa      240

agtggtaatg agcggtggcg caatcgtgat gccgatcttt catgaaataa tccccatcaa      300

tatgaatact ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga      360

tcgtatgcgc gtcgatgtag tagtcgcttt ctttgtgcgg gtaaaacctt cagtagaagg      420

gattgccacc gctgcccaga cgctggggca acgcaccctg tcgcctgaag acttacgtat      480

gttggttgaa gataaatttg tcgatgccct ccgtgcaaca gctgcgcaaa tgaccatgca      540

tgagttacag gatacccgcg agaactttgt gcagggggtg caaaatacag tggcagaaga      600

cctgtcgaaa aacggtctgg aactggagag cgtttcactt accaacttta accagacctc      660

gaaagaacat ttcaatccga acaatgcctt tgacgccgaa ggtttaacca aactgactca      720

ggaaacagag cgccgtcgcc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt      780

gcgtgaaaaa aatcgcgatg cgctatcgcg caagctggag attgaacagc aagaagcgtt      840

tatgacgctt gagcaggagc agcaggttaa aacccgtact gccgaacaga atgcacgtat      900

tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acacgaattc tggctgaacg      960

acagattcag gaaacagaaa tcgaccgcga acaggccgtc cgctcaagaa aggttgaagc     1020

tgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgctaacca     1080

gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc     1140

taatctcgca cttgcagaag cggtaagcgc ccaacaaaac gtagaaacca ctcgccagac     1200

tgccgaagcc gatcgtgcta aacaagttgc cctaatcgct gccgcgcagg atgcagaaac     1260

caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag agatgcaggc     1320

ggcggctatc gttgagttag ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc     1380

acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt     1440

caaactggcc ttgttgcagg cgctgcctgc ggtaatagag aaatccgttg agccgatgaa     1500

gtcaatcgac ggtatcaaga ttattcaggt cgatggtctg aatcgtggcg gcgctgcggg     1560

tgatgcaaac acgggtaatg tggggggcgg aaacctggcg gaacaagcat tatcagccgc     1620

tctctcttac cgcacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc     1680

aggcggctca ctggcggcat tgacttcacc cttaacctca acaactcccg tcgaagaaaa     1740

agcagagtcg gtacctggat ccgaaaacct gtacttccag ggtcaattca gcaaaggaga     1800

agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca     1860

caaattttct gtcagtggag agggtgaagg tgatgctaca tacggaaaac tcacccttaa     1920

atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactctgac     1980

ctatggtgtt caatgctttt cccgttatcc ggatcacatg aaacggcatg actttttcaa     2040

gagtgccatg cccgaaggtt atgtacagga acgcactata tctttcaaag atgacgggaa     2100

ctacaagacg cgtgctgaag tcaagtttga aggtgatacc cttgttaatc gtatcgagtt     2160

aaagggtatt gattttaaag aagatggaaa cattctcgga cacaaactag agtacaacta     2220

taactcacac aatgtataca tcacggcaga caaacaaaag aatggaatca aagctaactt     2280

caaaattcgc cacaacattg aagatggttc cgttcaacta gcagaccatt atcaacaaaa     2340

tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt cgacacaatc     2400

tgccctttcg aaagatccca acgaaaagcg tgaccacatg gtccttcttg agtttgtaac     2460

tgctgctggg attacacatg gcatggatga gctctacaaa aagcttgcgg cccatcatca     2520

tcaccaccac caccactgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc     2580

tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag     2640

gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc     2700

ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc     2760

gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt     2820

ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac     2880

ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag     2940

acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa     3000

actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg     3060

atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac     3120

aaaatattaa cgtttacaat ttcaggtggc acttttcggg gaaatgtgcg cggaacccct     3180

atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa     3240

aaactcatcg agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata     3300

tttttgaaaa agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat     3360

ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa     3420

tttcccctcg tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc     3480

cggtgagaat ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt     3540

acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg     3600

agcgagacga aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa     3660

ccggcgcagg aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc     3720

taatacctgg aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg     3780

agtacggata aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct     3840

gaccatctca tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc     3900

tggcgcatcg ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc     3960

gcgagcccat ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctaga     4020

gcaagacgtt tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc     4080

agacagtttt attgttcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt     4140

cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct     4200

gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc     4260

taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc     4320

ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc     4380

tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg     4440

ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt     4500

cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg     4560

agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg     4620

gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt     4680

atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag     4740

gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt     4800

gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta     4860

ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt     4920

cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg     4980

gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt     5040

aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg     5100

ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa     5160

gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc     5220

gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc     5280

tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata     5340

aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg     5400

ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg     5460

gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta     5520

tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca     5580

gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg     5640

gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt     5700

catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt     5760

atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac     5820

gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat ggcctgcttc     5880

tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt     5940

ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg     6000

aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc     6060

ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag     6120

gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt     6180

gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga     6240

atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt     6300

caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag     6360

caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg     6420

cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc     6480

aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc     6540

aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc     6600

ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag     6660

atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa     6720

cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc     6780

ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc     6840

cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt     6900

aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc     6960

gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga     7020

tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc     7080

aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag     7140

ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt     7200

caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt     7260

tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc     7320

gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact     7380

cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga     7440

atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac     7500

ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga     7560

tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc     7620

gtccggcgta gaggatcgag atctcgatcc cgcgaaat                             7658


<210>  65
<211>  2451
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(2451)
<223>  Membrane protein YqiK-GFP-His8

<400>  65
atg gat gat att gtt aat tct gtg ccc tcc tgg atg ttt acc gca att         48
Met Asp Asp Ile Val Asn Ser Val Pro Ser Trp Met Phe Thr Ala Ile           
1               5                   10                  15                

att gcc gta tgc att ctg ttt att att gga att att ttc gcc agg ctc         96
Ile Ala Val Cys Ile Leu Phe Ile Ile Gly Ile Ile Phe Ala Arg Leu           
            20                  25                  30                    

tat cgt cgc gct tcg gca gag caa gct ttt gtt cgt act ggt tta ggt        144
Tyr Arg Arg Ala Ser Ala Glu Gln Ala Phe Val Arg Thr Gly Leu Gly           
        35                  40                  45                        

ggg caa aaa gtg gta atg agc ggt ggc gca atc gtg atg ccg atc ttt        192
Gly Gln Lys Val Val Met Ser Gly Gly Ala Ile Val Met Pro Ile Phe           
    50                  55                  60                            

cat gaa ata atc ccc atc aat atg aat act ctg aag ctg gaa gtc agc        240
His Glu Ile Ile Pro Ile Asn Met Asn Thr Leu Lys Leu Glu Val Ser           
65                  70                  75                  80            

cgc tca acc att gat agc ctg att acg aaa gat cgt atg cgc gtc gat        288
Arg Ser Thr Ile Asp Ser Leu Ile Thr Lys Asp Arg Met Arg Val Asp           
                85                  90                  95                

gta gta gtc gct ttc ttt gtg cgg gta aaa cct tca gta gaa ggg att        336
Val Val Val Ala Phe Phe Val Arg Val Lys Pro Ser Val Glu Gly Ile           
            100                 105                 110                   

gcc acc gct gcc cag acg ctg ggg caa cgc acc ctg tcg cct gaa gac        384
Ala Thr Ala Ala Gln Thr Leu Gly Gln Arg Thr Leu Ser Pro Glu Asp           
        115                 120                 125                       

tta cgt atg ttg gtt gaa gat aaa ttt gtc gat gcc ctc cgt gca aca        432
Leu Arg Met Leu Val Glu Asp Lys Phe Val Asp Ala Leu Arg Ala Thr           
    130                 135                 140                           

gct gcg caa atg acc atg cat gag tta cag gat acc cgc gag aac ttt        480
Ala Ala Gln Met Thr Met His Glu Leu Gln Asp Thr Arg Glu Asn Phe           
145                 150                 155                 160           

gtg cag ggg gtg caa aat aca gtg gca gaa gac ctg tcg aaa aac ggt        528
Val Gln Gly Val Gln Asn Thr Val Ala Glu Asp Leu Ser Lys Asn Gly           
                165                 170                 175               

ctg gaa ctg gag agc gtt tca ctt acc aac ttt aac cag acc tcg aaa        576
Leu Glu Leu Glu Ser Val Ser Leu Thr Asn Phe Asn Gln Thr Ser Lys           
            180                 185                 190                   

gaa cat ttc aat ccg aac aat gcc ttt gac gcc gaa ggt tta acc aaa        624
Glu His Phe Asn Pro Asn Asn Ala Phe Asp Ala Glu Gly Leu Thr Lys           
        195                 200                 205                       

ctg act cag gaa aca gag cgc cgt cgc cgc gaa cgt aac gaa gtt gaa        672
Leu Thr Gln Glu Thr Glu Arg Arg Arg Arg Glu Arg Asn Glu Val Glu           
    210                 215                 220                           

cag gat gta gaa gtt gcg gtg cgt gaa aaa aat cgc gat gcg cta tcg        720
Gln Asp Val Glu Val Ala Val Arg Glu Lys Asn Arg Asp Ala Leu Ser           
225                 230                 235                 240           

cgc aag ctg gag att gaa cag caa gaa gcg ttt atg acg ctt gag cag        768
Arg Lys Leu Glu Ile Glu Gln Gln Glu Ala Phe Met Thr Leu Glu Gln           
                245                 250                 255               

gag cag cag gtt aaa acc cgt act gcc gaa cag aat gca cgt att gcg        816
Glu Gln Gln Val Lys Thr Arg Thr Ala Glu Gln Asn Ala Arg Ile Ala           
            260                 265                 270                   

gct ttt gaa gct gaa cgt cgt cgt gaa gca gag cag aca cga att ctg        864
Ala Phe Glu Ala Glu Arg Arg Arg Glu Ala Glu Gln Thr Arg Ile Leu           
        275                 280                 285                       

gct gaa cga cag att cag gaa aca gaa atc gac cgc gaa cag gcc gtc        912
Ala Glu Arg Gln Ile Gln Glu Thr Glu Ile Asp Arg Glu Gln Ala Val           
    290                 295                 300                           

cgc tca aga aag gtt gaa gct gaa cgt gaa gtt cgc att aaa gag atc        960
Arg Ser Arg Lys Val Glu Ala Glu Arg Glu Val Arg Ile Lys Glu Ile           
305                 310                 315                 320           

gaa cag cag cag gtc acc gaa atc gct aac cag acg aaa tcg atc gct       1008
Glu Gln Gln Gln Val Thr Glu Ile Ala Asn Gln Thr Lys Ser Ile Ala           
                325                 330                 335               

att gcc gcc aaa tcg gaa caa cag tcc cag gca gaa gcg cgt gct aat       1056
Ile Ala Ala Lys Ser Glu Gln Gln Ser Gln Ala Glu Ala Arg Ala Asn           
            340                 345                 350                   

ctc gca ctt gca gaa gcg gta agc gcc caa caa aac gta gaa acc act       1104
Leu Ala Leu Ala Glu Ala Val Ser Ala Gln Gln Asn Val Glu Thr Thr           
        355                 360                 365                       

cgc cag act gcc gaa gcc gat cgt gct aaa caa gtt gcc cta atc gct       1152
Arg Gln Thr Ala Glu Ala Asp Arg Ala Lys Gln Val Ala Leu Ile Ala           
    370                 375                 380                           

gcc gcg cag gat gca gaa acc aaa gcg gtt gaa ctg acc gtg cgg gcg       1200
Ala Ala Gln Asp Ala Glu Thr Lys Ala Val Glu Leu Thr Val Arg Ala           
385                 390                 395                 400           

aaa gca gaa aaa gaa gcc gca gag atg cag gcg gcg gct atc gtt gag       1248
Lys Ala Glu Lys Glu Ala Ala Glu Met Gln Ala Ala Ala Ile Val Glu           
                405                 410                 415               

tta gcc gaa gct aca cgt aaa aag ggt ctg gcg gaa gca gaa gca caa       1296
Leu Ala Glu Ala Thr Arg Lys Lys Gly Leu Ala Glu Ala Glu Ala Gln           
            420                 425                 430                   

cgt gcg ctg aac gat gct atc aac gta ctt tct gat gaa caa acc agc       1344
Arg Ala Leu Asn Asp Ala Ile Asn Val Leu Ser Asp Glu Gln Thr Ser           
        435                 440                 445                       

ctt aaa ttc aaa ctg gcc ttg ttg cag gcg ctg cct gcg gta ata gag       1392
Leu Lys Phe Lys Leu Ala Leu Leu Gln Ala Leu Pro Ala Val Ile Glu           
    450                 455                 460                           

aaa tcc gtt gag ccg atg aag tca atc gac ggt atc aag att att cag       1440
Lys Ser Val Glu Pro Met Lys Ser Ile Asp Gly Ile Lys Ile Ile Gln           
465                 470                 475                 480           

gtc gat ggt ctg aat cgt ggc ggc gct gcg ggt gat gca aac acg ggt       1488
Val Asp Gly Leu Asn Arg Gly Gly Ala Ala Gly Asp Ala Asn Thr Gly           
                485                 490                 495               

aat gtg ggg ggc gga aac ctg gcg gaa caa gca tta tca gcc gct ctc       1536
Asn Val Gly Gly Gly Asn Leu Ala Glu Gln Ala Leu Ser Ala Ala Leu           
            500                 505                 510                   

tct tac cgc aca cag gca ccg ctg att gac tcc ttg ctc aat gaa att       1584
Ser Tyr Arg Thr Gln Ala Pro Leu Ile Asp Ser Leu Leu Asn Glu Ile           
        515                 520                 525                       

ggc gtt tca ggc ggc tca ctg gcg gca ttg act tca ccc tta acc tca       1632
Gly Val Ser Gly Gly Ser Leu Ala Ala Leu Thr Ser Pro Leu Thr Ser           
    530                 535                 540                           

aca act ccc gtc gaa gaa aaa gca gag tcg gta cct gga tcc gaa aac       1680
Thr Thr Pro Val Glu Glu Lys Ala Glu Ser Val Pro Gly Ser Glu Asn           
545                 550                 555                 560           

ctg tac ttc cag ggt caa ttc agc aaa gga gaa gaa ctt ttc act gga       1728
Leu Tyr Phe Gln Gly Gln Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly           
                565                 570                 575               

gtt gtc cca att ctt gtt gaa tta gat ggt gat gtt aat ggg cac aaa       1776
Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys           
            580                 585                 590                   

ttt tct gtc agt gga gag ggt gaa ggt gat gct aca tac gga aaa ctc       1824
Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu           
        595                 600                 605                       

acc ctt aaa ttt att tgc act act gga aaa cta cct gtt cca tgg cca       1872
Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro           
    610                 615                 620                           

aca ctt gtc act act ctg acc tat ggt gtt caa tgc ttt tcc cgt tat       1920
Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr           
625                 630                 635                 640           

ccg gat cac atg aaa cgg cat gac ttt ttc aag agt gcc atg ccc gaa       1968
Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu           
                645                 650                 655               

ggt tat gta cag gaa cgc act ata tct ttc aaa gat gac ggg aac tac       2016
Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr           
            660                 665                 670                   

aag acg cgt gct gaa gtc aag ttt gaa ggt gat acc ctt gtt aat cgt       2064
Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg           
        675                 680                 685                       

atc gag tta aag ggt att gat ttt aaa gaa gat gga aac att ctc gga       2112
Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly           
    690                 695                 700                           

cac aaa cta gag tac aac tat aac tca cac aat gta tac atc acg gca       2160
His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala           
705                 710                 715                 720           

gac aaa caa aag aat gga atc aaa gct aac ttc aaa att cgc cac aac       2208
Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn           
                725                 730                 735               

att gaa gat ggt tcc gtt caa cta gca gac cat tat caa caa aat act       2256
Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr           
            740                 745                 750                   

cca att ggc gat ggc cct gtc ctt tta cca gac aac cat tac ctg tcg       2304
Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser           
        755                 760                 765                       

aca caa tct gcc ctt tcg aaa gat ccc aac gaa aag cgt gac cac atg       2352
Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met           
    770                 775                 780                           

gtc ctt ctt gag ttt gta act gct gct ggg att aca cat ggc atg gat       2400
Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp           
785                 790                 795                 800           

gag ctc tac aaa aag ctt gcg gcc cat cat cat cac cac cac cac cac       2448
Glu Leu Tyr Lys Lys Leu Ala Ala His His His His His His His His           
                805                 810                 815               

tga                                                                   2451


<210>  66
<211>  816
<212>  PRT
<213>  synthetic

<400>  66

Met Asp Asp Ile Val Asn Ser Val Pro Ser Trp Met Phe Thr Ala Ile 
1               5                   10                  15      


Ile Ala Val Cys Ile Leu Phe Ile Ile Gly Ile Ile Phe Ala Arg Leu 
            20                  25                  30          


Tyr Arg Arg Ala Ser Ala Glu Gln Ala Phe Val Arg Thr Gly Leu Gly 
        35                  40                  45              


Gly Gln Lys Val Val Met Ser Gly Gly Ala Ile Val Met Pro Ile Phe 
    50                  55                  60                  


His Glu Ile Ile Pro Ile Asn Met Asn Thr Leu Lys Leu Glu Val Ser 
65                  70                  75                  80  


Arg Ser Thr Ile Asp Ser Leu Ile Thr Lys Asp Arg Met Arg Val Asp 
                85                  90                  95      


Val Val Val Ala Phe Phe Val Arg Val Lys Pro Ser Val Glu Gly Ile 
            100                 105                 110         


Ala Thr Ala Ala Gln Thr Leu Gly Gln Arg Thr Leu Ser Pro Glu Asp 
        115                 120                 125             


Leu Arg Met Leu Val Glu Asp Lys Phe Val Asp Ala Leu Arg Ala Thr 
    130                 135                 140                 


Ala Ala Gln Met Thr Met His Glu Leu Gln Asp Thr Arg Glu Asn Phe 
145                 150                 155                 160 


Val Gln Gly Val Gln Asn Thr Val Ala Glu Asp Leu Ser Lys Asn Gly 
                165                 170                 175     


Leu Glu Leu Glu Ser Val Ser Leu Thr Asn Phe Asn Gln Thr Ser Lys 
            180                 185                 190         


Glu His Phe Asn Pro Asn Asn Ala Phe Asp Ala Glu Gly Leu Thr Lys 
        195                 200                 205             


Leu Thr Gln Glu Thr Glu Arg Arg Arg Arg Glu Arg Asn Glu Val Glu 
    210                 215                 220                 


Gln Asp Val Glu Val Ala Val Arg Glu Lys Asn Arg Asp Ala Leu Ser 
225                 230                 235                 240 


Arg Lys Leu Glu Ile Glu Gln Gln Glu Ala Phe Met Thr Leu Glu Gln 
                245                 250                 255     


Glu Gln Gln Val Lys Thr Arg Thr Ala Glu Gln Asn Ala Arg Ile Ala 
            260                 265                 270         


Ala Phe Glu Ala Glu Arg Arg Arg Glu Ala Glu Gln Thr Arg Ile Leu 
        275                 280                 285             


Ala Glu Arg Gln Ile Gln Glu Thr Glu Ile Asp Arg Glu Gln Ala Val 
    290                 295                 300                 


Arg Ser Arg Lys Val Glu Ala Glu Arg Glu Val Arg Ile Lys Glu Ile 
305                 310                 315                 320 


Glu Gln Gln Gln Val Thr Glu Ile Ala Asn Gln Thr Lys Ser Ile Ala 
                325                 330                 335     


Ile Ala Ala Lys Ser Glu Gln Gln Ser Gln Ala Glu Ala Arg Ala Asn 
            340                 345                 350         


Leu Ala Leu Ala Glu Ala Val Ser Ala Gln Gln Asn Val Glu Thr Thr 
        355                 360                 365             


Arg Gln Thr Ala Glu Ala Asp Arg Ala Lys Gln Val Ala Leu Ile Ala 
    370                 375                 380                 


Ala Ala Gln Asp Ala Glu Thr Lys Ala Val Glu Leu Thr Val Arg Ala 
385                 390                 395                 400 


Lys Ala Glu Lys Glu Ala Ala Glu Met Gln Ala Ala Ala Ile Val Glu 
                405                 410                 415     


Leu Ala Glu Ala Thr Arg Lys Lys Gly Leu Ala Glu Ala Glu Ala Gln 
            420                 425                 430         


Arg Ala Leu Asn Asp Ala Ile Asn Val Leu Ser Asp Glu Gln Thr Ser 
        435                 440                 445             


Leu Lys Phe Lys Leu Ala Leu Leu Gln Ala Leu Pro Ala Val Ile Glu 
    450                 455                 460                 


Lys Ser Val Glu Pro Met Lys Ser Ile Asp Gly Ile Lys Ile Ile Gln 
465                 470                 475                 480 


Val Asp Gly Leu Asn Arg Gly Gly Ala Ala Gly Asp Ala Asn Thr Gly 
                485                 490                 495     


Asn Val Gly Gly Gly Asn Leu Ala Glu Gln Ala Leu Ser Ala Ala Leu 
            500                 505                 510         


Ser Tyr Arg Thr Gln Ala Pro Leu Ile Asp Ser Leu Leu Asn Glu Ile 
        515                 520                 525             


Gly Val Ser Gly Gly Ser Leu Ala Ala Leu Thr Ser Pro Leu Thr Ser 
    530                 535                 540                 


Thr Thr Pro Val Glu Glu Lys Ala Glu Ser Val Pro Gly Ser Glu Asn 
545                 550                 555                 560 


Leu Tyr Phe Gln Gly Gln Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly 
                565                 570                 575     


Val Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys 
            580                 585                 590         


Phe Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu 
        595                 600                 605             


Thr Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro 
    610                 615                 620                 


Thr Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr 
625                 630                 635                 640 


Pro Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu 
                645                 650                 655     


Gly Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr 
            660                 665                 670         


Lys Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg 
        675                 680                 685             


Ile Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly 
    690                 695                 700                 


His Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala 
705                 710                 715                 720 


Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn 
                725                 730                 735     


Ile Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr 
            740                 745                 750         


Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser 
        755                 760                 765             


Thr Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met 
    770                 775                 780                 


Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp 
785                 790                 795                 800 


Glu Leu Tyr Lys Lys Leu Ala Ala His His His His His His His His 
                805                 810                 815     


<210>  67
<211>  8471
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(8471)
<223>  Plasmid pYidC

<400>  67
taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt       60

tgtttaactt taagaaggag accagtttat ggattcgcaa cgcaatcttt tagtcatcgc      120

tttgctgttc gtgtctttca tgatctggca agcctgggag caggataaaa acccgcaacc      180

tcaggcccaa cagaccacgc agacaacgac caccgcagcg ggtagcgccg ccgaccaggg      240

cgtaccggcc agtggccagg ggaaactgat ctcggttaag accgacgtgc ttgatctgac      300

catcaacacc cgtggtggtg atgttgagca agctctgctg cctgcttacc cgaaagagct      360

gaactctacc cagccgttcc agctgttgga aacttcaccg cagtttattt atcaggcaca      420

gagcggtctg accggtcgtg atggcccgga taacccggct aacggcccgc gtccgctgta      480

taacgttgaa aaagacgctt atgtgctggc tgaaggtcaa aacgaactgc aggtgccgat      540

gacgtatacc gacgcggcag gcaacacgtt taccaaaacg tttgtcctga aacgtggtga      600

ttacgctgtc aacgtcaact acaacgtgca gaacgctggc gagaaaccgc tggaaatctc      660

ctcgtttggt cagttgaagc aatccatcac tctgccaccg catctcgata ccggaagcag      720

caacttcgca ctgcacacct tccgtggcgc ggcgtactcc acgcctgacg agaagtatga      780

gaaatacaag ttcgatacca ttgccgataa cgaaaacctg aacatctctt cgaaaggtgg      840

ttgggtggcg atgctgcaac agtatttcgc gacggcgtgg atcccgcata acgacggtac      900

caacaacttc tataccgcta atctgggtaa cggcatcgcc gctatcggct ataaatctca      960

gccggtactg gttcagcctg gtcagactgg cgcgatgaac agcaccctgt gggttggccc     1020

ggaaatccag gacaaaatgg cagctgttgc tccgcacctg gatctgaccg ttgattacgg     1080

ttggttgtgg ttcatctctc agccgctgtt caaactgctg aaatggatcc atagctttgt     1140

gggtaactgg ggcttctcca ttatcatcat cacctttatc gttcgtggca tcatgtaccc     1200

gctgaccaaa gcgcagtaca cctccatggc gaagatgcgt atgttgcagc cgaagattca     1260

ggcaatgcgt gagcgtctgg gcgatgacaa acagcgtatc agccaggaaa tgatggcgct     1320

gtacaaagct gagaaggtta acccgctggg cggctgcttc ccgctgctga tccagatgcc     1380

aatcttcctg gcgttgtact acatgctgat gggttccgtt gaactgcgtc aggcaccgtt     1440

tgcactgtgg atccacgacc tgtcggcaca ggacccgtac tacatcctgc cgatcctgat     1500

gggcgtaacg atgttcttca ttcagaagat gtcgccgacc acagtgaccg acccgatgca     1560

gcagaagatc atgaccttta tgccggtcat cttcaccgtg ttcttcctgt ggttcccgtc     1620

aggtctggtg ctgtactata tcgtcagcaa cctggtaacc attattcagc agcagctgat     1680

ttaccgtggt ctggaaaaac gtggcctgca tagccgcgag aagaaaaaat ccgaattcag     1740

caaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt     1800

taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgctacat acggaaaact     1860

cacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac     1920

tactctgacc tatggtgttc aatgcttttc ccgttatccg gatcacatga aacggcatga     1980

ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga     2040

tgacgggaac tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg     2100

tatcgagtta aagggtattg attttaaaga agatggaaac attctcggac acaaactaga     2160

gtacaactat aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa     2220

agctaacttc aaaattcgcc acaacattga agatggttcc gttcaactag cagaccatta     2280

tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc     2340

gacacaatct gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga     2400

gtttgtaact gctgctggga ttacacatgg catggatgag ctctacaaat tgggtaccct     2460

cgagcaccac caccaccacc actgacatag gaggtcctcc tatgtcaatt caacatttcc     2520

gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa     2580

cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac     2640

tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga     2700

tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag     2760

agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca     2820

cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca     2880

tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa     2940

ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc     3000

tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa     3060

cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag     3120

actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct     3180

ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac     3240

tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa     3300

ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt     3360

aactaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc     3420

ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat     3480

atccggattg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg     3540

gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc     3600

ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc     3660

cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt     3720

gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag     3780

tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg     3840

gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag     3900

ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcaggt     3960

ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca     4020

aatatgtatc cgctcatgaa ttaattctta gaaaaactca tcgagcatca aatgaaactg     4080

caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga     4140

aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat     4200

tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc     4260

aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat     4320

ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc     4380

aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt     4440

aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc     4500

aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttcccggg     4560

gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg     4620

aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa catcattggc     4680

aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg     4740

atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc     4800

agcatccatg ttggaattta atcgcggcct agagcaagac gtttcccgtt gaatatggct     4860

cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgaccaaaa     4920

tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat     4980

cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc     5040

taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg     5100

gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc     5160

acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg     5220

ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg     5280

ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa     5340

cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg     5400

aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga     5460

gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct     5520

gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca     5580

gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc     5640

ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg     5700

ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc     5760

tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac     5820

tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta     5880

cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg     5940

gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg     6000

tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca     6060

gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt     6120

ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt     6180

tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata     6240

ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta     6300

ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc     6360

actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag     6420

cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc     6480

agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt     6540

ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta     6600

aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt     6660

ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca     6720

gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc     6780

atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc     6840

tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc     6900

cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgagatccc     6960

ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag     7020

tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt     7080

ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt     7140

gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag     7200

caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt     7260

atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc     7320

gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc     7380

ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg     7440

ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag     7500

acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc     7560

gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat     7620

gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac     7680

agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc     7740

gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac     7800

caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg     7860

cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag     7920

ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc     7980

ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga     8040

gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa     8100

ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt     8160

gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta     8220

ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca     8280

acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc     8340

cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg     8400

cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga     8460

tcccgcgaaa t                                                          8471


<210>  68
<211>  2397
<212>  DNA
<213>  Synthetic


<220>
<221>  CDS
<222>  (1)..(2397)
<223>  Membrane protein YidC-GFP-His8

<400>  68
atg gat tcg caa cgc aat ctt tta gtc atc gct ttg ctg ttc gtg tct         48
Met Asp Ser Gln Arg Asn Leu Leu Val Ile Ala Leu Leu Phe Val Ser           
1               5                   10                  15                

ttc atg atc tgg caa gcc tgg gag cag gat aaa aac ccg caa cct cag         96
Phe Met Ile Trp Gln Ala Trp Glu Gln Asp Lys Asn Pro Gln Pro Gln           
            20                  25                  30                    

gcc caa cag acc acg cag aca acg acc acc gca gcg ggt agc gcc gcc        144
Ala Gln Gln Thr Thr Gln Thr Thr Thr Thr Ala Ala Gly Ser Ala Ala           
        35                  40                  45                        

gac cag ggc gta ccg gcc agt ggc cag ggg aaa ctg atc tcg gtt aag        192
Asp Gln Gly Val Pro Ala Ser Gly Gln Gly Lys Leu Ile Ser Val Lys           
    50                  55                  60                            

acc gac gtg ctt gat ctg acc atc aac acc cgt ggt ggt gat gtt gag        240
Thr Asp Val Leu Asp Leu Thr Ile Asn Thr Arg Gly Gly Asp Val Glu           
65                  70                  75                  80            

caa gct ctg ctg cct gct tac ccg aaa gag ctg aac tct acc cag ccg        288
Gln Ala Leu Leu Pro Ala Tyr Pro Lys Glu Leu Asn Ser Thr Gln Pro           
                85                  90                  95                

ttc cag ctg ttg gaa act tca ccg cag ttt att tat cag gca cag agc        336
Phe Gln Leu Leu Glu Thr Ser Pro Gln Phe Ile Tyr Gln Ala Gln Ser           
            100                 105                 110                   

ggt ctg acc ggt cgt gat ggc ccg gat aac ccg gct aac ggc ccg cgt        384
Gly Leu Thr Gly Arg Asp Gly Pro Asp Asn Pro Ala Asn Gly Pro Arg           
        115                 120                 125                       

ccg ctg tat aac gtt gaa aaa gac gct tat gtg ctg gct gaa ggt caa        432
Pro Leu Tyr Asn Val Glu Lys Asp Ala Tyr Val Leu Ala Glu Gly Gln           
    130                 135                 140                           

aac gaa ctg cag gtg ccg atg acg tat acc gac gcg gca ggc aac acg        480
Asn Glu Leu Gln Val Pro Met Thr Tyr Thr Asp Ala Ala Gly Asn Thr           
145                 150                 155                 160           

ttt acc aaa acg ttt gtc ctg aaa cgt ggt gat tac gct gtc aac gtc        528
Phe Thr Lys Thr Phe Val Leu Lys Arg Gly Asp Tyr Ala Val Asn Val           
                165                 170                 175               

aac tac aac gtg cag aac gct ggc gag aaa ccg ctg gaa atc tcc tcg        576
Asn Tyr Asn Val Gln Asn Ala Gly Glu Lys Pro Leu Glu Ile Ser Ser           
            180                 185                 190                   

ttt ggt cag ttg aag caa tcc atc act ctg cca ccg cat ctc gat acc        624
Phe Gly Gln Leu Lys Gln Ser Ile Thr Leu Pro Pro His Leu Asp Thr           
        195                 200                 205                       

gga agc agc aac ttc gca ctg cac acc ttc cgt ggc gcg gcg tac tcc        672
Gly Ser Ser Asn Phe Ala Leu His Thr Phe Arg Gly Ala Ala Tyr Ser           
    210                 215                 220                           

acg cct gac gag aag tat gag aaa tac aag ttc gat acc att gcc gat        720
Thr Pro Asp Glu Lys Tyr Glu Lys Tyr Lys Phe Asp Thr Ile Ala Asp           
225                 230                 235                 240           

aac gaa aac ctg aac atc tct tcg aaa ggt ggt tgg gtg gcg atg ctg        768
Asn Glu Asn Leu Asn Ile Ser Ser Lys Gly Gly Trp Val Ala Met Leu           
                245                 250                 255               

caa cag tat ttc gcg acg gcg tgg atc ccg cat aac gac ggt acc aac        816
Gln Gln Tyr Phe Ala Thr Ala Trp Ile Pro His Asn Asp Gly Thr Asn           
            260                 265                 270                   

aac ttc tat acc gct aat ctg ggt aac ggc atc gcc gct atc ggc tat        864
Asn Phe Tyr Thr Ala Asn Leu Gly Asn Gly Ile Ala Ala Ile Gly Tyr           
        275                 280                 285                       

aaa tct cag ccg gta ctg gtt cag cct ggt cag act ggc gcg atg aac        912
Lys Ser Gln Pro Val Leu Val Gln Pro Gly Gln Thr Gly Ala Met Asn           
    290                 295                 300                           

agc acc ctg tgg gtt ggc ccg gaa atc cag gac aaa atg gca gct gtt        960
Ser Thr Leu Trp Val Gly Pro Glu Ile Gln Asp Lys Met Ala Ala Val           
305                 310                 315                 320           

gct ccg cac ctg gat ctg acc gtt gat tac ggt tgg ttg tgg ttc atc       1008
Ala Pro His Leu Asp Leu Thr Val Asp Tyr Gly Trp Leu Trp Phe Ile           
                325                 330                 335               

tct cag ccg ctg ttc aaa ctg ctg aaa tgg atc cat agc ttt gtg ggt       1056
Ser Gln Pro Leu Phe Lys Leu Leu Lys Trp Ile His Ser Phe Val Gly           
            340                 345                 350                   

aac tgg ggc ttc tcc att atc atc atc acc ttt atc gtt cgt ggc atc       1104
Asn Trp Gly Phe Ser Ile Ile Ile Ile Thr Phe Ile Val Arg Gly Ile           
        355                 360                 365                       

atg tac ccg ctg acc aaa gcg cag tac acc tcc atg gcg aag atg cgt       1152
Met Tyr Pro Leu Thr Lys Ala Gln Tyr Thr Ser Met Ala Lys Met Arg           
    370                 375                 380                           

atg ttg cag ccg aag att cag gca atg cgt gag cgt ctg ggc gat gac       1200
Met Leu Gln Pro Lys Ile Gln Ala Met Arg Glu Arg Leu Gly Asp Asp           
385                 390                 395                 400           

aaa cag cgt atc agc cag gaa atg atg gcg ctg tac aaa gct gag aag       1248
Lys Gln Arg Ile Ser Gln Glu Met Met Ala Leu Tyr Lys Ala Glu Lys           
                405                 410                 415               

gtt aac ccg ctg ggc ggc tgc ttc ccg ctg ctg atc cag atg cca atc       1296
Val Asn Pro Leu Gly Gly Cys Phe Pro Leu Leu Ile Gln Met Pro Ile           
            420                 425                 430                   

ttc ctg gcg ttg tac tac atg ctg atg ggt tcc gtt gaa ctg cgt cag       1344
Phe Leu Ala Leu Tyr Tyr Met Leu Met Gly Ser Val Glu Leu Arg Gln           
        435                 440                 445                       

gca ccg ttt gca ctg tgg atc cac gac ctg tcg gca cag gac ccg tac       1392
Ala Pro Phe Ala Leu Trp Ile His Asp Leu Ser Ala Gln Asp Pro Tyr           
    450                 455                 460                           

tac atc ctg ccg atc ctg atg ggc gta acg atg ttc ttc att cag aag       1440
Tyr Ile Leu Pro Ile Leu Met Gly Val Thr Met Phe Phe Ile Gln Lys           
465                 470                 475                 480           

atg tcg ccg acc aca gtg acc gac ccg atg cag cag aag atc atg acc       1488
Met Ser Pro Thr Thr Val Thr Asp Pro Met Gln Gln Lys Ile Met Thr           
                485                 490                 495               

ttt atg ccg gtc atc ttc acc gtg ttc ttc ctg tgg ttc ccg tca ggt       1536
Phe Met Pro Val Ile Phe Thr Val Phe Phe Leu Trp Phe Pro Ser Gly           
            500                 505                 510                   

ctg gtg ctg tac tat atc gtc agc aac ctg gta acc att att cag cag       1584
Leu Val Leu Tyr Tyr Ile Val Ser Asn Leu Val Thr Ile Ile Gln Gln           
        515                 520                 525                       

cag ctg att tac cgt ggt ctg gaa aaa cgt ggc ctg cat agc cgc gag       1632
Gln Leu Ile Tyr Arg Gly Leu Glu Lys Arg Gly Leu His Ser Arg Glu           
    530                 535                 540                           

aag aaa aaa tcc gaa ttc agc aaa gga gaa gaa ctt ttc act gga gtt       1680
Lys Lys Lys Ser Glu Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly Val           
545                 550                 555                 560           

gtc cca att ctt gtt gaa tta gat ggt gat gtt aat ggg cac aaa ttt       1728
Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe           
                565                 570                 575               

tct gtc agt gga gag ggt gaa ggt gat gct aca tac gga aaa ctc acc       1776
Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr           
            580                 585                 590                   

ctt aaa ttt att tgc act act gga aaa cta cct gtt cca tgg cca aca       1824
Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr           
        595                 600                 605                       

ctt gtc act act ctg acc tat ggt gtt caa tgc ttt tcc cgt tat ccg       1872
Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro           
    610                 615                 620                           

gat cac atg aaa cgg cat gac ttt ttc aag agt gcc atg ccc gaa ggt       1920
Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly           
625                 630                 635                 640           

tat gta cag gaa cgc act ata tct ttc aaa gat gac ggg aac tac aag       1968
Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys           
                645                 650                 655               

acg cgt gct gaa gtc aag ttt gaa ggt gat acc ctt gtt aat cgt atc       2016
Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile           
            660                 665                 670                   

gag tta aag ggt att gat ttt aaa gaa gat gga aac att ctc gga cac       2064
Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His           
        675                 680                 685                       

aaa cta gag tac aac tat aac tca cac aat gta tac atc acg gca gac       2112
Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp           
    690                 695                 700                           

aaa caa aag aat gga atc aaa gct aac ttc aaa att cgc cac aac att       2160
Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile           
705                 710                 715                 720           

gaa gat ggt tcc gtt caa cta gca gac cat tat caa caa aat act cca       2208
Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro           
                725                 730                 735               

att ggc gat ggc cct gtc ctt tta cca gac aac cat tac ctg tcg aca       2256
Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr           
            740                 745                 750                   

caa tct gcc ctt tcg aaa gat ccc aac gaa aag cgt gac cac atg gtc       2304
Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val           
        755                 760                 765                       

ctt ctt gag ttt gta act gct gct ggg att aca cat ggc atg gat gag       2352
Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu           
    770                 775                 780                           

ctc tac aaa ttg ggt acc ctc gag cac cac cac cac cac cac tga           2397
Leu Tyr Lys Leu Gly Thr Leu Glu His His His His His His                   
785                 790                 795                               


<210>  69
<211>  798
<212>  PRT
<213>  Synthetic

<400>  69

Met Asp Ser Gln Arg Asn Leu Leu Val Ile Ala Leu Leu Phe Val Ser 
1               5                   10                  15      


Phe Met Ile Trp Gln Ala Trp Glu Gln Asp Lys Asn Pro Gln Pro Gln 
            20                  25                  30          


Ala Gln Gln Thr Thr Gln Thr Thr Thr Thr Ala Ala Gly Ser Ala Ala 
        35                  40                  45              


Asp Gln Gly Val Pro Ala Ser Gly Gln Gly Lys Leu Ile Ser Val Lys 
    50                  55                  60                  


Thr Asp Val Leu Asp Leu Thr Ile Asn Thr Arg Gly Gly Asp Val Glu 
65                  70                  75                  80  


Gln Ala Leu Leu Pro Ala Tyr Pro Lys Glu Leu Asn Ser Thr Gln Pro 
                85                  90                  95      


Phe Gln Leu Leu Glu Thr Ser Pro Gln Phe Ile Tyr Gln Ala Gln Ser 
            100                 105                 110         


Gly Leu Thr Gly Arg Asp Gly Pro Asp Asn Pro Ala Asn Gly Pro Arg 
        115                 120                 125             


Pro Leu Tyr Asn Val Glu Lys Asp Ala Tyr Val Leu Ala Glu Gly Gln 
    130                 135                 140                 


Asn Glu Leu Gln Val Pro Met Thr Tyr Thr Asp Ala Ala Gly Asn Thr 
145                 150                 155                 160 


Phe Thr Lys Thr Phe Val Leu Lys Arg Gly Asp Tyr Ala Val Asn Val 
                165                 170                 175     


Asn Tyr Asn Val Gln Asn Ala Gly Glu Lys Pro Leu Glu Ile Ser Ser 
            180                 185                 190         


Phe Gly Gln Leu Lys Gln Ser Ile Thr Leu Pro Pro His Leu Asp Thr 
        195                 200                 205             


Gly Ser Ser Asn Phe Ala Leu His Thr Phe Arg Gly Ala Ala Tyr Ser 
    210                 215                 220                 


Thr Pro Asp Glu Lys Tyr Glu Lys Tyr Lys Phe Asp Thr Ile Ala Asp 
225                 230                 235                 240 


Asn Glu Asn Leu Asn Ile Ser Ser Lys Gly Gly Trp Val Ala Met Leu 
                245                 250                 255     


Gln Gln Tyr Phe Ala Thr Ala Trp Ile Pro His Asn Asp Gly Thr Asn 
            260                 265                 270         


Asn Phe Tyr Thr Ala Asn Leu Gly Asn Gly Ile Ala Ala Ile Gly Tyr 
        275                 280                 285             


Lys Ser Gln Pro Val Leu Val Gln Pro Gly Gln Thr Gly Ala Met Asn 
    290                 295                 300                 


Ser Thr Leu Trp Val Gly Pro Glu Ile Gln Asp Lys Met Ala Ala Val 
305                 310                 315                 320 


Ala Pro His Leu Asp Leu Thr Val Asp Tyr Gly Trp Leu Trp Phe Ile 
                325                 330                 335     


Ser Gln Pro Leu Phe Lys Leu Leu Lys Trp Ile His Ser Phe Val Gly 
            340                 345                 350         


Asn Trp Gly Phe Ser Ile Ile Ile Ile Thr Phe Ile Val Arg Gly Ile 
        355                 360                 365             


Met Tyr Pro Leu Thr Lys Ala Gln Tyr Thr Ser Met Ala Lys Met Arg 
    370                 375                 380                 


Met Leu Gln Pro Lys Ile Gln Ala Met Arg Glu Arg Leu Gly Asp Asp 
385                 390                 395                 400 


Lys Gln Arg Ile Ser Gln Glu Met Met Ala Leu Tyr Lys Ala Glu Lys 
                405                 410                 415     


Val Asn Pro Leu Gly Gly Cys Phe Pro Leu Leu Ile Gln Met Pro Ile 
            420                 425                 430         


Phe Leu Ala Leu Tyr Tyr Met Leu Met Gly Ser Val Glu Leu Arg Gln 
        435                 440                 445             


Ala Pro Phe Ala Leu Trp Ile His Asp Leu Ser Ala Gln Asp Pro Tyr 
    450                 455                 460                 


Tyr Ile Leu Pro Ile Leu Met Gly Val Thr Met Phe Phe Ile Gln Lys 
465                 470                 475                 480 


Met Ser Pro Thr Thr Val Thr Asp Pro Met Gln Gln Lys Ile Met Thr 
                485                 490                 495     


Phe Met Pro Val Ile Phe Thr Val Phe Phe Leu Trp Phe Pro Ser Gly 
            500                 505                 510         


Leu Val Leu Tyr Tyr Ile Val Ser Asn Leu Val Thr Ile Ile Gln Gln 
        515                 520                 525             


Gln Leu Ile Tyr Arg Gly Leu Glu Lys Arg Gly Leu His Ser Arg Glu 
    530                 535                 540                 


Lys Lys Lys Ser Glu Phe Ser Lys Gly Glu Glu Leu Phe Thr Gly Val 
545                 550                 555                 560 


Val Pro Ile Leu Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe 
                565                 570                 575     


Ser Val Ser Gly Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr 
            580                 585                 590         


Leu Lys Phe Ile Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr 
        595                 600                 605             


Leu Val Thr Thr Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro 
    610                 615                 620                 


Asp His Met Lys Arg His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly 
625                 630                 635                 640 


Tyr Val Gln Glu Arg Thr Ile Ser Phe Lys Asp Asp Gly Asn Tyr Lys 
                645                 650                 655     


Thr Arg Ala Glu Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile 
            660                 665                 670         


Glu Leu Lys Gly Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His 
        675                 680                 685             


Lys Leu Glu Tyr Asn Tyr Asn Ser His Asn Val Tyr Ile Thr Ala Asp 
    690                 695                 700                 


Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg His Asn Ile 
705                 710                 715                 720 


Glu Asp Gly Ser Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro 
                725                 730                 735     


Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr 
            740                 745                 750         


Gln Ser Ala Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val 
        755                 760                 765             


Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu 
    770                 775                 780                 


Leu Tyr Lys Leu Gly Thr Leu Glu His His His His His His 
785                 790                 795             


<210>  70
<211>  25
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(25)
<223>  hp6

<400>  70
tgacatagga ggtcctccta tgtca                                             25


<210>  71
<211>  861
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(861)
<223>  AmpR

<400>  71
atg tca att caa cat ttc cgt gtc gcc ctt att ccc ttt ttt gcg gca         48
Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala           
1               5                   10                  15                

ttt tgc ctt cct gtt ttt gct cac cca gaa acg ctg gtg aaa gta aaa         96
Phe Cys Leu Pro Val Phe Ala His Pro Glu Thr Leu Val Lys Val Lys           
            20                  25                  30                    

gat gct gaa gat cag ttg ggt gca cga gtg ggt tac atc gaa ctg gat        144
Asp Ala Glu Asp Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp           
        35                  40                  45                        

ctc aac agc ggt aag atc ctt gag agt ttt cgc ccc gaa gaa cgt ttt        192
Leu Asn Ser Gly Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe           
    50                  55                  60                            

cca atg atg agc act ttt aaa gtt ctg cta tgt ggc gcg gta tta tcc        240
Pro Met Met Ser Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser           
65                  70                  75                  80            

cgt gtt gac gcc ggg caa gag caa ctc ggt cgc cgc ata cac tat tct        288
Arg Val Asp Ala Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser           
                85                  90                  95                

cag aat gac ttg gtt gag tac tca cca gtc aca gaa aag cat ctt acg        336
Gln Asn Asp Leu Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr           
            100                 105                 110                   

gat ggc atg aca gta aga gaa tta tgc agt gct gcc ata acc atg agt        384
Asp Gly Met Thr Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser           
        115                 120                 125                       

gat aac act gcg gcc aac tta ctt ctg aca acg atc gga gga ccg aag        432
Asp Asn Thr Ala Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys           
    130                 135                 140                           

gag cta acc gct ttt ttg cac aac atg ggg gat cat gta act cgc ctt        480
Glu Leu Thr Ala Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu           
145                 150                 155                 160           

gat cgt tgg gaa ccg gag ctg aat gaa gcc ata cca aac gac gag cgt        528
Asp Arg Trp Glu Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg           
                165                 170                 175               

gac acc acg atg cct gca gca atg gca aca acg ttg cgc aaa cta tta        576
Asp Thr Thr Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu           
            180                 185                 190                   

act ggc gaa cta ctt act cta gct tcc cgg caa caa tta ata gac tgg        624
Thr Gly Glu Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp           
        195                 200                 205                       

atg gag gcg gat aaa gtt gca gga cca ctt ctg cgc tcg gcc ctt ccg        672
Met Glu Ala Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro           
    210                 215                 220                           

gct ggc tgg ttt att gct gat aaa tct gga gcc ggt gag cgt ggg tct        720
Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser           
225                 230                 235                 240           

cgc ggt atc att gca gca ctg ggg cca gat ggt aag ccc tcc cgt atc        768
Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile           
                245                 250                 255               

gta gtt atc tac acg acg ggg agt cag gca act atg gat gaa cga aat        816
Val Val Ile Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn           
            260                 265                 270                   

aga cag atc gct gag ata ggt gcc tca ctg att aag cat tgg taa            861
Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp                   
        275                 280                 285                       


<210>  72
<211>  286
<212>  PRT
<213>  synthetic

<400>  72

Met Ser Ile Gln His Phe Arg Val Ala Leu Ile Pro Phe Phe Ala Ala 
1               5                   10                  15      


Phe Cys Leu Pro Val Phe Ala His Pro Glu Thr Leu Val Lys Val Lys 
            20                  25                  30          


Asp Ala Glu Asp Gln Leu Gly Ala Arg Val Gly Tyr Ile Glu Leu Asp 
        35                  40                  45              


Leu Asn Ser Gly Lys Ile Leu Glu Ser Phe Arg Pro Glu Glu Arg Phe 
    50                  55                  60                  


Pro Met Met Ser Thr Phe Lys Val Leu Leu Cys Gly Ala Val Leu Ser 
65                  70                  75                  80  


Arg Val Asp Ala Gly Gln Glu Gln Leu Gly Arg Arg Ile His Tyr Ser 
                85                  90                  95      


Gln Asn Asp Leu Val Glu Tyr Ser Pro Val Thr Glu Lys His Leu Thr 
            100                 105                 110         


Asp Gly Met Thr Val Arg Glu Leu Cys Ser Ala Ala Ile Thr Met Ser 
        115                 120                 125             


Asp Asn Thr Ala Ala Asn Leu Leu Leu Thr Thr Ile Gly Gly Pro Lys 
    130                 135                 140                 


Glu Leu Thr Ala Phe Leu His Asn Met Gly Asp His Val Thr Arg Leu 
145                 150                 155                 160 


Asp Arg Trp Glu Pro Glu Leu Asn Glu Ala Ile Pro Asn Asp Glu Arg 
                165                 170                 175     


Asp Thr Thr Met Pro Ala Ala Met Ala Thr Thr Leu Arg Lys Leu Leu 
            180                 185                 190         


Thr Gly Glu Leu Leu Thr Leu Ala Ser Arg Gln Gln Leu Ile Asp Trp 
        195                 200                 205             


Met Glu Ala Asp Lys Val Ala Gly Pro Leu Leu Arg Ser Ala Leu Pro 
    210                 215                 220                 


Ala Gly Trp Phe Ile Ala Asp Lys Ser Gly Ala Gly Glu Arg Gly Ser 
225                 230                 235                 240 


Arg Gly Ile Ile Ala Ala Leu Gly Pro Asp Gly Lys Pro Ser Arg Ile 
                245                 250                 255     


Val Val Ile Tyr Thr Thr Gly Ser Gln Ala Thr Met Asp Glu Arg Asn 
            260                 265                 270         


Arg Gln Ile Ala Glu Ile Gly Ala Ser Leu Ile Lys His Trp 
        275                 280                 285     


<210>  73
<211>  6728
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(6728)
<223>  Plasmid pMax-truncV489

<400>  73
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcaacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgaggtta cctagaaatc     4680

atccttagcg aaagctaagg atttttttta tctgtaatta attgaactca ctaaagggag     4740

accacagcgg tttccctttg ttcgcattgg aggtcaaata atgcgcaagt cttataaaca     4800

attctataag gctccgagga ggcatatcca agtgtgggag gcagccaatg ggccggatcc     4860

acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga     4920

gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa     4980

attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat     5040

ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat     5100

ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct     5160

gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct     5220

gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa     5280

gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga     5340

gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc     5400

ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga     5460

gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact     5520

aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg     5580

gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc     5640

gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat     5700

gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag     5760

agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     5820

ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa     5880

aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg     5940

aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc     6000

gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt     6060

gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat     6120

agtaagccag tatacactcc gctagcgctg atgtccggcg gtgcttttgc cgttacgcac     6180

caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc     6240

tcaaaaacac catcatacac taaatcagta agttggcagc atcacccgac gcactttgcg     6300

ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg     6360

ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa     6420

gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat     6480

cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg     6540

ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat     6600

gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa     6660

ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc     6720

cggaattc                                                              6728


<210>  74
<211>  1467
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(1467)
<223>  rneD346N truncated at V489

<400>  74
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc aac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agc       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtc tgc ccg cgc tgc tcc ggt acc ggt acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tat ctg ctg aat gaa aaa cgt tct gcg gtg aat gcc att gaa acg       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtt cgc tgc gtg att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg                                   1467
Thr Pro His Tyr His Val Leu Arg Val                                       
                485                                                       


<210>  75
<211>  489
<212>  PRT
<213>  synthetic

<400>  75

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val 
                485                 


<210>  76
<211>  6848
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(6848)
<223>  Plasmid pMax-truncL529

<400>  76
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcaacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctgaggtta cctagaaatc     4800

atccttagcg aaagctaagg atttttttta tctgtaatta attgaactca ctaaagggag     4860

accacagcgg tttccctttg ttcgcattgg aggtcaaata atgcgcaagt cttataaaca     4920

attctataag gctccgagga ggcatatcca agtgtgggag gcagccaatg ggccggatcc     4980

acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga     5040

gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa     5100

attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat     5160

ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat     5220

ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct     5280

gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct     5340

gtcaaacatg agaattacaa cttatatcgt atggggctga cttcaggtgc tacatttgaa     5400

gagataaatt gcactgaaat ctagaaatat tttatctgat taataagatg atcttcttga     5460

gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg aaaaaaccgc cttgcagggc     5520

ggtttttcga aggttctctg agctaccaac tctttgaacc gaggtaactg gcttggagga     5580

gcgcagtcac caaaacttgt cctttcagtt tagccttaac cggcgcatga cttcaagact     5640

aactcctcta aatcaattac cagtggctgc tgccagtggt gcttttgcat gtctttccgg     5700

gttggactca agacgatagt taccggataa ggcgcagcgg tcggactgaa cggggggttc     5760

gtgcatacag tccagcttgg agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat     5820

gagacaaacg cggccataac agcggaatga caccggtaaa ccgaaaggca ggaacaggag     5880

agcgcacgag ggagccgcca ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg     5940

ccaccactga tttgagcgtc agatttcgtg atgcttgtca ggggggcgga gcctatggaa     6000

aaacggcttt gccgcggccc tctcacttcc ctgttaagta tcttcctggc atcttccagg     6060

aaatctccgc cccgttcgta agccatttcc gctcgccgca gtcgaacgac cgagcgtagc     6120

gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca tattctgctg acgcaccggt     6180

gcagcctttt ttctcctgcc acatgaagca cttcactgac accctcatca gtgccaacat     6240

agtaagccag tatacactcc gctagcgctg atgtccggcg gtgcttttgc cgttacgcac     6300

caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca ctggagcacc     6360

tcaaaaacac catcatacac taaatcagta agttggcagc atcacccgac gcactttgcg     6420

ccgaataaat acctgtgacg gaagatcact tcgcagaata aataaatcct ggtgtccctg     6480

ttgataccgg gaagccctgg gccaactttt ggcgaaaatg agacgttgat cggcacgtaa     6540

gaggttccaa ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat     6600

cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg     6660

ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat     6720

gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa     6780

ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc     6840

cggaattc                                                              6848


<210>  77
<211>  1587
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(1587)
<223>  rneD346N truncated at L529

<400>  77
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc aac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agc       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtc tgc ccg cgc tgc tcc ggt acc ggt acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tat ctg ctg aat gaa aaa cgt tct gcg gtg aat gcc att gaa acg       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtt cgc tgc gtg att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg cgt aaa ggg gaa gaa acg ccg       1488
Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro           
                485                 490                 495               

acc tta agc tac atg ctg ccg aag ctg cat gaa gaa gcg atg gcg ctg       1536
Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu           
            500                 505                 510                   

ccg tct gaa gaa gag ttc gct gaa cgt aag cgt ccg gaa caa cct gcg       1584
Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala           
        515                 520                 525                       

ctg                                                                   1587
Leu                                                                       
                                                                          


<210>  78
<211>  529
<212>  PRT
<213>  synthetic

<400>  78

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asn Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu 
    


<210>  79
<211>  8447
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(8447)
<223>  Plasmid pMax-D346X, wherein NNN may be TTT, CTG, AAT, GTG, AGC, 
       CCG, ACC, GCG, TAT, CAT, CAG, AAA, GAA, TGC, or GGC

<400>  79
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcnnnttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc     4800

cggatgtgcc gccagcgcca accccagctg aacctgccgc gcctgtcgta gccccagcac     4860

ctaaatctgc accggcaaca ccagccgctc ctgcccaacc tgggctgttg agccgcttct     4920

tcggcgcact gaaagcgctg ttcagcggtg gtgaagaaac caaaccgtcc gagcaaccaa     4980

caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca     5040

accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata     5100

atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg     5160

agagccgtca gcaggttgag gtaacggaaa aagcgcgtac caccgacgag cagcaagcgc     5220

cgcgtcgtga acgtagccgc cgccgtaatg atgataaacg tcaggcgcaa caagaagcga     5280

aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc     5340

cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg     5400

tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgtcgctgcc gaaccaattg     5460

ttcaggaagc gccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc     5520

aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc     5580

cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct     5640

atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc     5700

cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac     5760

aggttgaaga gcagcgcgaa caggaagaag tacatgtgca gccgatggtg actgaggtcc     5820

ctgtcgccgc cgctatcgaa ccggttgtta gcgcgccagt tgttgaagaa gtggccggtg     5880

tcgtagaagc ccccgttcag gttgccgaac cgcaaccgga agtggttgaa acgacgcatc     5940

ctgaagtgat tgctgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg     6000

ccgtagccca ggaagttgca gaacaagcag aaccggtggt tgaaccgcag gaagagacgg     6060

cagatattga agaagttgtc gaaactgctg aggttgtagt tgctgaacct gaagttgttg     6120

ctcaacctgc cgcgccagta gtcgctgaag tcgcagcaga agttgaaacg gtagctgcgg     6180

tcgaacctga ggtcaccgtt gagcataacc acgctaccgc gccaatgacg cgcgctccag     6240

caccggaata tgttccggag gcaccgcgtc acagtgactg gcagcgccct acttttgcct     6300

tcgaaggtaa aggtgccgca ggtggtcata cggcaacaca tcatgcctct gctgctcctg     6360

cgcgtccgca acctgttgag taaaggttac ctagaaatca tccttagcga aagctaagga     6420

ttttttttat ctgtaattaa ttgaactcac taaagggaga ccacagcggt ttccctttgt     6480

tcgcattgga ggtcaaataa tgcgcaagtc ttataaacaa ttctataagg ctccgaggag     6540

gcatatccaa gtgtgggagg cagccaatgg gccggatcca caggacgggt gtggtcgcca     6600

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa     6660

agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg     6720

ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc     6780

ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga     6840

tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact     6900

gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattacaac     6960

ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc     7020

tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta     7080

atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga     7140

gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc     7200

ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc     7260

agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt     7320

accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga     7380

gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca     7440

gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag     7500

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca     7560

gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct     7620

ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa     7680

gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg     7740

gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca     7800

catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg     7860

ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca     7920

ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact     7980

aaatcagtaa gttggcagca tcacccgacg cactttgcgc cgaataaata cctgtgacgg     8040

aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg     8100

ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata     8160

atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg     8220

aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc     8280

gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc     8340

agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg     8400

cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattc                   8447


<210>  80
<211>  1061
<212>  PRT
<213>  E. coli


<220>
<221>  PEPTIDE
<222>  (1)..(1061)
<223>  Translation of rneD346X, wherien X is Phe(F), Leu(L), Ile(I), 
       Val(V), Ser(S), Pro(P), Thr(T), Ala(A), Tyr(Y), HIs(H), Gln(Q), 
       Lys(K), Glu(E), Cyc(C), or Gly(G).

<400>  80

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Xaa Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu 
            580                 585                 590         


Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg 
        595                 600                 605             


Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp 
    610                 615                 620                 


Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg 
625                 630                 635                 640 


Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser 
                645                 650                 655     


Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln 
            660                 665                 670         


Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg 
        675                 680                 685             


Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Ser Val 
    690                 695                 700                 


Gln Glu Thr Glu Gln Glu Glu Arg Val Arg Pro Val Gln Pro Arg Arg 
705                 710                 715                 720 


Lys Gln Arg Gln Leu Asn Gln Lys Val Arg Tyr Glu Gln Ser Val Ala 
                725                 730                 735     


Glu Glu Ala Val Val Ala Pro Val Val Glu Glu Thr Val Ala Ala Glu 
            740                 745                 750         


Pro Ile Val Gln Glu Ala Pro Ala Pro Arg Thr Glu Leu Val Lys Val 
        755                 760                 765             


Pro Leu Pro Val Val Ala Gln Thr Ala Pro Glu Gln Gln Glu Glu Asn 
    770                 775                 780                 


Asn Ala Asp Asn Arg Asp Asn Gly Gly Met Pro Arg Arg Ser Arg Arg 
785                 790                 795                 800 


Ser Pro Arg His Leu Arg Val Ser Gly Gln Arg Arg Arg Arg Tyr Arg 
                805                 810                 815     


Asp Glu Arg Tyr Pro Thr Gln Ser Pro Met Pro Leu Thr Val Ala Cys 
            820                 825                 830         


Ala Ser Pro Glu Leu Ala Ser Gly Lys Val Trp Ile Arg Tyr Pro Ile 
        835                 840                 845             


Val Arg Pro Gln Asp Val Gln Val Glu Glu Gln Arg Glu Gln Glu Glu 
    850                 855                 860                 


Val His Val Gln Pro Met Val Thr Glu Val Pro Val Ala Ala Ala Ile 
865                 870                 875                 880 


Glu Pro Val Val Ser Ala Pro Val Val Glu Glu Val Ala Gly Val Val 
                885                 890                 895     


Glu Ala Pro Val Gln Val Ala Glu Pro Gln Pro Glu Val Val Glu Thr 
            900                 905                 910         


Thr His Pro Glu Val Ile Ala Ala Ala Val Thr Glu Gln Pro Gln Val 
        915                 920                 925             


Ile Thr Glu Ser Asp Val Ala Val Ala Gln Glu Val Ala Glu Gln Ala 
    930                 935                 940                 


Glu Pro Val Val Glu Pro Gln Glu Glu Thr Ala Asp Ile Glu Glu Val 
945                 950                 955                 960 


Val Glu Thr Ala Glu Val Val Val Ala Glu Pro Glu Val Val Ala Gln 
                965                 970                 975     


Pro Ala Ala Pro Val Val Ala Glu Val Ala Ala Glu Val Glu Thr Val 
            980                 985                 990         


Ala Ala Val Glu Pro Glu Val Thr  Val Glu His Asn His  Ala Thr Ala 
        995                 1000                 1005             


Pro Met  Thr Arg Ala Pro Ala  Pro Glu Tyr Val Pro  Glu Ala Pro 
    1010                 1015                 1020             


Arg His  Ser Asp Trp Gln Arg  Pro Thr Phe Ala Phe  Glu Gly Lys 
    1025                 1030                 1035             


Gly Ala  Ala Gly Gly His Thr  Ala Thr His His Ala  Ser Ala Ala 
    1040                 1045                 1050             


Pro Ala  Arg Pro Gln Pro Val  Glu 
    1055                 1060     


<210>  81
<211>  8447
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(8447)
<223>  pMax-rneWT

<400>  81
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc     4800

cggatgtgcc gccagcgcca accccagctg aacctgccgc gcctgtcgta gccccagcac     4860

ctaaatctgc accggcaaca ccagccgctc ctgcccaacc tgggctgttg agccgcttct     4920

tcggcgcact gaaagcgctg ttcagcggtg gtgaagaaac caaaccgtcc gagcaaccaa     4980

caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca     5040

accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata     5100

atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg     5160

agagccgtca gcaggttgag gtaacggaaa aagcgcgtac caccgacgag cagcaagcgc     5220

cgcgtcgtga acgtagccgc cgccgtaatg atgataaacg tcaggcgcaa caagaagcga     5280

aggcgctgaa tgttgaagag caatctgttc aggaaaccga acaggaagaa cgtgtacgtc     5340

cggttcagcc gcgtcgtaaa cagcgtcagc tcaatcagaa agtgcgttac gagcaaagcg     5400

tagccgaaga agcggtagtc gcaccggtgg ttgaagaaac tgtcgctgcc gaaccaattg     5460

ttcaggaagc gccagctcca cgcacagaac tggtgaaagt cccgctgcca gtcgtagcgc     5520

aaactgcacc agaacagcaa gaagagaaca atgctgataa ccgtgacaac ggtggcatgc     5580

cgcgtcgttc tcgccgctcg cctcgtcacc tgcgcgtaag tggtcagcgt cgtcgtcgct     5640

atcgtgacga gcgttatcca acccagtcgc caatgccgtt gaccgtagcg tgcgcgtctc     5700

cggaactggc ctctggcaaa gtctggatcc gctatccaat tgtacgtccg caagatgtac     5760

aggttgaaga gcagcgcgaa caggaagaag tacatgtgca gccgatggtg actgaggtcc     5820

ctgtcgccgc cgctatcgaa ccggttgtta gcgcgccagt tgttgaagaa gtggccggtg     5880

tcgtagaagc ccccgttcag gttgccgaac cgcaaccgga agtggttgaa acgacgcatc     5940

ctgaagtgat tgctgccgcg gtaactgaac agccgcaggt gattaccgag tctgatgttg     6000

ccgtagccca ggaagttgca gaacaagcag aaccggtggt tgaaccgcag gaagagacgg     6060

cagatattga agaagttgtc gaaactgctg aggttgtagt tgctgaacct gaagttgttg     6120

ctcaacctgc cgcgccagta gtcgctgaag tcgcagcaga agttgaaacg gtagctgcgg     6180

tcgaacctga ggtcaccgtt gagcataacc acgctaccgc gccaatgacg cgcgctccag     6240

caccggaata tgttccggag gcaccgcgtc acagtgactg gcagcgccct acttttgcct     6300

tcgaaggtaa aggtgccgca ggtggtcata cggcaacaca tcatgcctct gctgctcctg     6360

cgcgtccgca acctgttgag taaaggttac ctagaaatca tccttagcga aagctaagga     6420

ttttttttat ctgtaattaa ttgaactcac taaagggaga ccacagcggt ttccctttgt     6480

tcgcattgga ggtcaaataa tgcgcaagtc ttataaacaa ttctataagg ctccgaggag     6540

gcatatccaa gtgtgggagg cagccaatgg gccggatcca caggacgggt gtggtcgcca     6600

tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa     6660

agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg     6720

ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc     6780

ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga     6840

tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact     6900

gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattacaac     6960

ttatatcgta tggggctgac ttcaggtgct acatttgaag agataaattg cactgaaatc     7020

tagaaatatt ttatctgatt aataagatga tcttcttgag atcgttttgg tctgcgcgta     7080

atctcttgct ctgaaaacga aaaaaccgcc ttgcagggcg gtttttcgaa ggttctctga     7140

gctaccaact ctttgaaccg aggtaactgg cttggaggag cgcagtcacc aaaacttgtc     7200

ctttcagttt agccttaacc ggcgcatgac ttcaagacta actcctctaa atcaattacc     7260

agtggctgct gccagtggtg cttttgcatg tctttccggg ttggactcaa gacgatagtt     7320

accggataag gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccagcttgga     7380

gcgaactgcc tacccggaac tgagtgtcag gcgtggaatg agacaaacgc ggccataaca     7440

gcggaatgac accggtaaac cgaaaggcag gaacaggaga gcgcacgagg gagccgccag     7500

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc caccactgat ttgagcgtca     7560

gatttcgtga tgcttgtcag gggggcggag cctatggaaa aacggctttg ccgcggccct     7620

ctcacttccc tgttaagtat cttcctggca tcttccagga aatctccgcc ccgttcgtaa     7680

gccatttccg ctcgccgcag tcgaacgacc gagcgtagcg agtcagtgag cgaggaagcg     7740

gaatatatcc tgtatcacat attctgctga cgcaccggtg cagccttttt tctcctgcca     7800

catgaagcac ttcactgaca ccctcatcag tgccaacata gtaagccagt atacactccg     7860

ctagcgctga tgtccggcgg tgcttttgcc gttacgcacc accccgtcag tagctgaaca     7920

ggagggacag ctgatagaaa cagaagccac tggagcacct caaaaacacc atcatacact     7980

aaatcagtaa gttggcagca tcacccgacg cactttgcgc cgaataaata cctgtgacgg     8040

aagatcactt cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg     8100

ccaacttttg gcgaaaatga gacgttgatc ggcacgtaag aggttccaac tttcaccata     8160

atgaaataag atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg     8220

aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc     8280

gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc     8340

agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg     8400

cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattc                   8447


<210>  82
<211>  7112
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(7112)
<223>  pMax-rneSTAR

<400>  82
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagtcat cacgtttgtc cgcgttgttc tggtactggc accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttacctg ctgaatgaaa     4560

aacgttctgc ggtaaatgcc attgaaactc gtcaggacgg tgtgcgctgt gtaattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

ccccaacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc     4800

cggatgtgcc gcctgcgcca acgccagctg aacctgccgc gcctgttgta gctccagcac     4860

cgaaagctgc accggcaaca ccagcagctc ctgcacaacc tgggctgttg agccgcttct     4920

tcggcgcact gaaagcgctg ttcagcggtg gaagaaacca aaccgaccga gcaaccagca     4980

ccgaaagcag aagcgaaacc ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac     5040

cgccgtgaag gttacctaga aatcatcctt agcgaaagct aaggattttt tttatctgta     5100

attaattgaa ctcactaaag ggagaccaca gcggtttccc tttgttcgca ttggaggtca     5160

aataatgcgc aagtcttata aacaattcta taaggctccg aggaggcata tccaagtgtg     5220

ggaggcagcc aatgggccgg atccacagga cgggtgtggt cgccatgatc gcgtagtcga     5280

tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg     5340

ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat     5400

agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca     5460

taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat     5520

tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc     5580

attaaagctt atcgatgata agctgtcaaa catgagaatt acaacttata tcgtatgggg     5640

ctgacttcag gtgctacatt tgaagagata aattgcactg aaatctagaa atattttatc     5700

tgattaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa     5760

aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg     5820

aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct     5880

taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag     5940

tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca     6000

gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc     6060

ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg     6120

taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggaa acgcctggta     6180

tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagattt cgtgatgctt     6240

gtcagggggg cggagcctat ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta     6300

agtatcttcc tggcatcttc caggaaatct ccgccccgtt cgtaagccat ttccgctcgc     6360

cgcagtcgaa cgaccgagcg tagcgagtca gtgagcgagg aagcggaata tatcctgtat     6420

cacatattct gctgacgcac cggtgcagcc ttttttctcc tgccacatga agcacttcac     6480

tgacaccctc atcagtgcca acatagtaag ccagtataca ctccgctagc gctgatgtcc     6540

ggcggtgctt ttgccgttac gcaccacccc gtcagtagct gaacaggagg gacagctgat     6600

agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc agtaagttgg     6660

cagcatcacc cgacgcactt tgcgccgaat aaatacctgt gacggaagat cacttcgcag     6720

aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa     6780

aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac     6840

taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga     6900

aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg     6960

aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg     7020

cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc     7080

ttgcccgcct gatgaatgct catccggaat tc                                   7112


<210>  83
<211>  1851
<212>  DNA
<213>  E. coli


<220>
<221>  CDS
<222>  (1)..(1851)
<223>  rneSTAR encoding a truncated RNAse E

<400>  83
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc gac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agt       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtt tgt ccg cgt tgt tct ggt act ggc acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tac ctg ctg aat gaa aaa cgt tct gcg gta aat gcc att gaa act       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtg cgc tgt gta att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg cgt aaa ggg gaa gaa acc cca       1488
Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro           
                485                 490                 495               

acc tta agc tac atg ctg ccg aag ctg cat gaa gaa gcg atg gcg ctg       1536
Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu           
            500                 505                 510                   

ccg tct gaa gaa gag ttc gct gaa cgt aag cgt ccg gaa caa cct gcg       1584
Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala           
        515                 520                 525                       

ctg gca acc ttt gcc atg ccg gat gtg ccg cct gcg cca acg cca gct       1632
Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala           
    530                 535                 540                           

gaa cct gcc gcg cct gtt gta gct cca gca ccg aaa gct gca ccg gca       1680
Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ala Ala Pro Ala           
545                 550                 555                 560           

aca cca gca gct cct gca caa cct ggg ctg ttg agc cgc ttc ttc ggc       1728
Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly           
                565                 570                 575               

gca ctg aaa gcg ctg ttc agc ggt gga aga aac caa acc gac cga gca       1776
Ala Leu Lys Ala Leu Phe Ser Gly Gly Arg Asn Gln Thr Asp Arg Ala           
            580                 585                 590                   

acc agc acc gaa agc aga agc gaa acc gga acg tca aca gga tcg tcg       1824
Thr Ser Thr Glu Ser Arg Ser Glu Thr Gly Thr Ser Thr Gly Ser Ser           
        595                 600                 605                       

caa gcc tcg tca gaa caa ccg ccg tga                                   1851
Gln Ala Ser Ser Glu Gln Pro Pro                                           
    610                 615                                               


<210>  84
<211>  616
<212>  PRT
<213>  E. coli

<400>  84

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ala Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Arg Asn Gln Thr Asp Arg Ala 
            580                 585                 590         


Thr Ser Thr Glu Ser Arg Ser Glu Thr Gly Thr Ser Thr Gly Ser Ser 
        595                 600                 605             


Gln Ala Ser Ser Glu Gln Pro Pro 
    610                 615     


<210>  85
<211>  9224
<212>  DNA
<213>  synthetic


<220>
<221>  misc_feature
<222>  (1)..(9224)
<223>  Plasmid pMac-rneEvo21

<400>  85
cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc       60

gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc      120

cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat      180

ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc      240

accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg      300

ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat      360

gccgtctgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat      420

gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt      480

gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga      540

cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac      600

cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt      660

tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca      720

gagcctgata aaaacggtta gcgcttcgtt aatacagatg taggtgttcc acagggtagc      780

cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgcttg tttcggcgtg      840

ggtatggtgg caggccccgt ggccggggga ctgttgggcg ctgccggcac ctgtcctacg      900

agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac      960

cggaaggagc taccggacag cggtgcggac tgttgtaact cagaataaga aatgaggccg     1020

ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt     1080

tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg     1140

aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt     1200

cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct     1260

agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg     1320

cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa     1380

gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg     1440

gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg     1500

caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca     1560

acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga     1620

tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat     1680

ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa     1740

gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca     1800

gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg     1860

gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc     1920

cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg     1980

gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca     2040

tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac     2100

gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga     2160

gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca     2220

cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc     2280

gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg     2340

tgatgccggc cacgatgcgt ccggcgtaga ggatccgggt cccctttgat agattaaaaa     2400

ggaaaggagg aaagaaataa tggctcgtgt acagtttaaa caacgtgaat ctactgacgc     2460

aatctttgtt cactgctcgg ctaccaagcc aagtcagaat gttggtgtcc gtgagattcg     2520

ccagtggcac aaagagcagg gttggctcga tgtgggatac cactttatca tcaagcgaga     2580

cggtactgtg gaggcaggac gagatgagat ggctgtaggc tctcacgcta agggttacaa     2640

ccacaactct atcggcgtct gccttgttgg tggtatcgac gataaaggta agttcgacgc     2700

taactttacg ccagcccaaa tgcaatccct tcgctcactg cttgtcacac tgctggctaa     2760

gtacgaaggc gctgtgcttc gcgcccatca tgaggtggcg ccgaaggctt gcccttcgtt     2820

cgaccttaag cgttggtggg agaagaacga actggtcact tctgaccgtg gataaaccgc     2880

tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgtt     2940

aattaagcgg ccgcggcgcg cccgtcgacg cgcgcgcggc tgaggatacc tcagccacca     3000

caattcagca aattgtgaac atcatcacgt tcatctttcc ctggttgcca atggcccatt     3060

ttcctgtcag taacgagaag gtcgcgaatt caggcgcttt ttagactggt cgtaatgaaa     3120

ttctttttaa gaaggagact atatatgaaa gcaattttcg tactgaaaca tcttaatcat     3180

gctaaggagg ttttctaatg aaaagaatgt taatcaacgc aactcagcag gaagagttgc     3240

gcgttgccct tgtagatggg cagcgtctgt atgacctgga tatcgaaagt ccagggcacg     3300

agcagaaaaa ggcaaacatc tacaaaggta aaatcacccg cattgaaccg agtctggaag     3360

ctgcttttgt tgattacggc gctgaacgtc acggtttcct cccactaaaa gaaattgccc     3420

gcgaatattt ccctgctaac tacagtgctc atggtcgtcc caacattaaa gatgtgttgc     3480

gtgaaggtca ggaagtcatt gttcagatcg ataaagaaga gcgcggcaac aaaggcgcgg     3540

cattaaccac ctttatcagt ctggcgggta gctatctggt tctgatgccg aacaacccgc     3600

gcgcgggtgg catttctcgc cgtatcgaag gcgacgaccg taccgaatta aaagaagcac     3660

tggcaagcct tgaactgccg gaaggcatgg ggcttatcgt gcgcaccgct ggcgtcggca     3720

aatctgctga ggcgctgcaa tgggatttaa gcttccgtct gaaacactgg gaagccatca     3780

aaaaagccgc tgaaagccgc ccggccccgt tcctgattca tcaggagagc aacgtaatcg     3840

ttcgcgcatt ccgcgattac ttacgtcagg acatcggcga aatccttatc gataacccga     3900

aagtgctcga actggcacgt cagcatatcg ctgcattagg tcgcccggat ttcagcagca     3960

aaatcaaact gtacaccggc gagatcccgc tgttcagcca ctaccagatc gagtcacaga     4020

tcgagtccgc cttccagcgt gaagttcgtc tgccgtctgg tggttccatt gttatcgaca     4080

gcaccgaagc gttaacggcc atcgacatca actccgcacg cgcgacccgc ggcggcgata     4140

tcgaagaaac cgcgtttaac actaacctcg aagctgccga tgagattgct cgtcagctgc     4200

gcctgcgtga cctcggcggc ctgattgtta tcgacttcat cgacatgacg ccagtacgcc     4260

accagcgtgc ggtagaaaac cgtctgcgtg aagcggtgcg tcaggaccgt gcgcgtattc     4320

aaatcagcca tatttctcgc tttggcctgc tggaaatgtc ccgtcagcgc ctgagcccat     4380

cactgggtga atccagccat cacgtctgcc cgcgctgctc cggtaccggt accgtgcgtg     4440

acaacgaatc gctgtcgctc tctattctgc gtctgatcga agaagaagcg ctgaaagaga     4500

acacccagga agttcacgcc attgttcctg tgccaatcgc ttcttatctg ctgaatgaaa     4560

aacgttctgc ggtgaatgcc attgaaacgc gtcaggacgg tgttcgctgc gtgattgtgc     4620

caaacgatca gatggaaacc ccgcactacc acgtgctgcg cgtgcgtaaa ggggaagaaa     4680

cgccgacctt aagctacatg ctgccgaagc tgcatgaaga agcgatggcg ctgccgtctg     4740

aagaagagtt cgctgaacgt aagcgtccgg aacaacctgc gctggcaacc tttgccatgc     4800

cggatgtgcc gccagcgcca accccagctg aacctgccgc gcctgtcgta gccccagcac     4860

ctaaatctgc accggcaaca ccagccgctc ctgcccaacc tgggctgttg agccgcttct     4920

tcggcgcact gaaagcgctg ttcagcggtg gtgaagaaac caaaccgtcc gagcaaccaa     4980

caccgaaagc agaagcgaaa ccggaacgtc aacaggatcg tcgcaagcct cgtcagaaca     5040

accgccgtga ccgtaatgag cgccgcgaca cccgtagtga acgtactgaa ggcagcgata     5100

atcgcgaaga aaaccgtcgt aatcgtcgcc aggcacagca gcagactgcc gagacgcgtg     5160

agagccgtca gcaggttgag gtaacggaaa aagcgcgtac caccgacgag cagcaagcgc     5220

cgcgtcgtga acgtagccgc cgccgtaatg atgataaacg tcaggcgcaa caagaagcga     5280

aggcgctgaa tgttgaagag caaggtaatg actccaactt attgatagtg ttttatgttc     5340

agataatgcc cgatgacttt gtcatgcagc tccaccgatt ttgagaacga cagcgacttc     5400

cgtcccagcc gtgccaggtg ctgcctcaga ttcaggttat gccgctcaat tcgctgcgta     5460

tatcgcttgc tgattacgtg cagctttccc ttcaggcggg attcatacag cggccagcca     5520

tccgtcatcc atatcaccac gtcaaagggt gacagcaggc tcataagacg ccccagcgtc     5580

gccatagtgc gttcaccgaa tacgtgcgca acaaccgtct tccggagact gtcatacgcg     5640

taaaacagcc agcgctggcg cgatttagcc ccgacatagc cccactgttc gtccatttcc     5700

gcgcagacga tgacgtcact gcccggctgt atgcgcgagg ttaccgactg cggcctgagt     5760

tttttaagtg acgtaaaatc gtgttgaggc caacgcccat aatgcgggct gttgcccggc     5820

atccaacgcc attcatggcc atatcaatga ttttctggtg cgtaccgggt tgagaagcgg     5880

tgtaagtgaa ctgcagttgc catgttttac ggcagtgaga gcagagatag cgctgatgtc     5940

cggcggtgct tttgccgtta cgcaccaccc cgtcagtagc tgaacaggag ggacagctga     6000

tagaaacaga agccactgga gcacctcaaa aacaccatca tacactaaat cagtaagttg     6060

gcagcatcac cgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg     6120

ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag     6180

ccgaagaagc ggtagtcgca ccggtggttg aagaaactgt cgctgccgaa ccaattgttc     6240

aggaagcgcc agctccacgc acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa     6300

ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc     6360

gtcgttctcg ccgctcgcct cgtcacctgc gcgtaagtgg tcagcgtcgt cgtcgctatc     6420

gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg     6480

aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa gatgtacagg     6540

ttgaagagca gcgcgaacag gaagaagtac atgtgcagcc gatggtgact gaggtccctg     6600

tcgccgccgc tatcgaaccg gttgttagcg cgccagttgt tgaagaagtg gccggtgtcg     6660

tagaagcccc cgttcaggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg     6720

aagtgattgc tgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg     6780

tagcccagga agttgcagaa caagcagaac cggtggttga accgcaggaa gagacggcag     6840

atattgaaga agttgtcgaa actgctgagg ttgtagttgc tgaacctgaa gttgttgctc     6900

aacctgccgc gccagtagtc gctgaagtcg cagcagaagt tgaaacggta gctgcggtcg     6960

aacctgaggt caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccagcac     7020

cggaatatgt tccggaggca ccgcgtcaca gtgactggca gcgccctact tttgccttcg     7080

aaggtaaagg tgccgcaggt ggtcatacgg caacacatca tgcctctgct gctcctgcgc     7140

gtccgcaacc tgttgagtaa aggttaccta gaaatcatcc ttagcgaaag ctaaggattt     7200

tttttatctg taattaattg aactcactaa agggagacca cagcggtttc cctttgttcg     7260

cattggaggt caaataatgc gcaagtctta taaacaattc tataaggctc cgaggaggca     7320

tatccaagtg tgggaggcag ccaatgggcc ggatccacag gacgggtgtg gtcgccatga     7380

tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc     7440

ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta     7500

gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg     7560

gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga     7620

cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg     7680

ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttacaactta     7740

tatcgtatgg ggctgacttc aggtgctaca tttgaagaga taaattgcac tgaaatctag     7800

aaatatttta tctgattaat aagatgatct tcttgagatc gttttggtct gcgcgtaatc     7860

tcttgctctg aaaacgaaaa aaccgccttg cagggcggtt tttcgaaggt tctctgagct     7920

accaactctt tgaaccgagg taactggctt ggaggagcgc agtcaccaaa acttgtcctt     7980

tcagtttagc cttaaccggc gcatgacttc aagactaact cctctaaatc aattaccagt     8040

ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg gactcaagac gatagttacc     8100

ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca gcttggagcg     8160

aactgcctac ccggaactga gtgtcaggcg tggaatgaga caaacgcggc cataacagcg     8220

gaatgacacc ggtaaaccga aaggcaggaa caggagagcg cacgagggag ccgccagggg     8280

aaacgcctgg tatctttata gtcctgtcgg gtttcgccac cactgatttg agcgtcagat     8340

ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac ggctttgccg cggccctctc     8400

acttccctgt taagtatctt cctggcatct tccaggaaat ctccgccccg ttcgtaagcc     8460

atttccgctc gccgcagtcg aacgaccgag cgtagcgagt cagtgagcga ggaagcggaa     8520

tatatcctgt atcacatatt ctgctgacgc accggtgcag ccttttttct cctgccacat     8580

gaagcacttc actgacaccc tcatcagtgc caacatagta agccagtata cactccgcta     8640

gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga     8700

gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa     8760

tcagtaagtt ggcagcatca cccgacgcac tttgcgccga ataaatacct gtgacggaag     8820

atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca     8880

acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg     8940

aaataagatc actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag     9000

ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa tggcatcgta     9060

aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag accgttcagc     9120

tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt tatccggcct     9180

ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attc                      9224


<210>  86
<211>  2187
<212>  DNA
<213>  synthetic


<220>
<221>  CDS
<222>  (1)..(2187)
<223>  rneEvo21 encoding truncated RNase E

<400>  86
atg aaa aga atg tta atc aac gca act cag cag gaa gag ttg cgc gtt         48
Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val           
1               5                   10                  15                

gcc ctt gta gat ggg cag cgt ctg tat gac ctg gat atc gaa agt cca         96
Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro           
            20                  25                  30                    

ggg cac gag cag aaa aag gca aac atc tac aaa ggt aaa atc acc cgc        144
Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg           
        35                  40                  45                        

att gaa ccg agt ctg gaa gct gct ttt gtt gat tac ggc gct gaa cgt        192
Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg           
    50                  55                  60                            

cac ggt ttc ctc cca cta aaa gaa att gcc cgc gaa tat ttc cct gct        240
His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala           
65                  70                  75                  80            

aac tac agt gct cat ggt cgt ccc aac att aaa gat gtg ttg cgt gaa        288
Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu           
                85                  90                  95                

ggt cag gaa gtc att gtt cag atc gat aaa gaa gag cgc ggc aac aaa        336
Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys           
            100                 105                 110                   

ggc gcg gca tta acc acc ttt atc agt ctg gcg ggt agc tat ctg gtt        384
Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val           
        115                 120                 125                       

ctg atg ccg aac aac ccg cgc gcg ggt ggc att tct cgc cgt atc gaa        432
Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu           
    130                 135                 140                           

ggc gac gac cgt acc gaa tta aaa gaa gca ctg gca agc ctt gaa ctg        480
Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu           
145                 150                 155                 160           

ccg gaa ggc atg ggg ctt atc gtg cgc acc gct ggc gtc ggc aaa tct        528
Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser           
                165                 170                 175               

gct gag gcg ctg caa tgg gat tta agc ttc cgt ctg aaa cac tgg gaa        576
Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu           
            180                 185                 190                   

gcc atc aaa aaa gcc gct gaa agc cgc ccg gcc ccg ttc ctg att cat        624
Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His           
        195                 200                 205                       

cag gag agc aac gta atc gtt cgc gca ttc cgc gat tac tta cgt cag        672
Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln           
    210                 215                 220                           

gac atc ggc gaa atc ctt atc gat aac ccg aaa gtg ctc gaa ctg gca        720
Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala           
225                 230                 235                 240           

cgt cag cat atc gct gca tta ggt cgc ccg gat ttc agc agc aaa atc        768
Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile           
                245                 250                 255               

aaa ctg tac acc ggc gag atc ccg ctg ttc agc cac tac cag atc gag        816
Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu           
            260                 265                 270                   

tca cag atc gag tcc gcc ttc cag cgt gaa gtt cgt ctg ccg tct ggt        864
Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly           
        275                 280                 285                       

ggt tcc att gtt atc gac agc acc gaa gcg tta acg gcc atc gac atc        912
Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile           
    290                 295                 300                           

aac tcc gca cgc gcg acc cgc ggc ggc gat atc gaa gaa acc gcg ttt        960
Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe           
305                 310                 315                 320           

aac act aac ctc gaa gct gcc gat gag att gct cgt cag ctg cgc ctg       1008
Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu           
                325                 330                 335               

cgt gac ctc ggc ggc ctg att gtt atc gac ttc atc gac atg acg cca       1056
Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro           
            340                 345                 350                   

gta cgc cac cag cgt gcg gta gaa aac cgt ctg cgt gaa gcg gtg cgt       1104
Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg           
        355                 360                 365                       

cag gac cgt gcg cgt att caa atc agc cat att tct cgc ttt ggc ctg       1152
Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu           
    370                 375                 380                           

ctg gaa atg tcc cgt cag cgc ctg agc cca tca ctg ggt gaa tcc agc       1200
Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser           
385                 390                 395                 400           

cat cac gtc tgc ccg cgc tgc tcc ggt acc ggt acc gtg cgt gac aac       1248
His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn           
                405                 410                 415               

gaa tcg ctg tcg ctc tct att ctg cgt ctg atc gaa gaa gaa gcg ctg       1296
Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu           
            420                 425                 430                   

aaa gag aac acc cag gaa gtt cac gcc att gtt cct gtg cca atc gct       1344
Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala           
        435                 440                 445                       

tct tat ctg ctg aat gaa aaa cgt tct gcg gtg aat gcc att gaa acg       1392
Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr           
    450                 455                 460                           

cgt cag gac ggt gtt cgc tgc gtg att gtg cca aac gat cag atg gaa       1440
Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu           
465                 470                 475                 480           

acc ccg cac tac cac gtg ctg cgc gtg cgt aaa ggg gaa gaa acg ccg       1488
Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro           
                485                 490                 495               

acc tta agc tac atg ctg ccg aag ctg cat gaa gaa gcg atg gcg ctg       1536
Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu           
            500                 505                 510                   

ccg tct gaa gaa gag ttc gct gaa cgt aag cgt ccg gaa caa cct gcg       1584
Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala           
        515                 520                 525                       

ctg gca acc ttt gcc atg ccg gat gtg ccg cca gcg cca acc cca gct       1632
Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala           
    530                 535                 540                           

gaa cct gcc gcg cct gtc gta gcc cca gca cct aaa tct gca ccg gca       1680
Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala           
545                 550                 555                 560           

aca cca gcc gct cct gcc caa cct ggg ctg ttg agc cgc ttc ttc ggc       1728
Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly           
                565                 570                 575               

gca ctg aaa gcg ctg ttc agc ggt ggt gaa gaa acc aaa ccg tcc gag       1776
Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu           
            580                 585                 590                   

caa cca aca ccg aaa gca gaa gcg aaa ccg gaa cgt caa cag gat cgt       1824
Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg           
        595                 600                 605                       

cgc aag cct cgt cag aac aac cgc cgt gac cgt aat gag cgc cgc gac       1872
Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp           
    610                 615                 620                           

acc cgt agt gaa cgt act gaa ggc agc gat aat cgc gaa gaa aac cgt       1920
Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg           
625                 630                 635                 640           

cgt aat cgt cgc cag gca cag cag cag act gcc gag acg cgt gag agc       1968
Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser           
                645                 650                 655               

cgt cag cag gtt gag gta acg gaa aaa gcg cgt acc acc gac gag cag       2016
Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln           
            660                 665                 670                   

caa gcg ccg cgt cgt gaa cgt agc cgc cgc cgt aat gat gat aaa cgt       2064
Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg           
        675                 680                 685                       

cag gcg caa caa gaa gcg aag gcg ctg aat gtt gaa gag caa ggt aat       2112
Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Gly Asn           
    690                 695                 700                           

gac tcc aac tta ttg ata gtg ttt tat gtt cag ata atg ccc gat gac       2160
Asp Ser Asn Leu Leu Ile Val Phe Tyr Val Gln Ile Met Pro Asp Asp           
705                 710                 715                 720           

ttt gtc atg cag ctc cac cga ttt tga                                   2187
Phe Val Met Gln Leu His Arg Phe                                           
                725                                                       


<210>  87
<211>  728
<212>  PRT
<213>  synthetic

<400>  87

Met Lys Arg Met Leu Ile Asn Ala Thr Gln Gln Glu Glu Leu Arg Val 
1               5                   10                  15      


Ala Leu Val Asp Gly Gln Arg Leu Tyr Asp Leu Asp Ile Glu Ser Pro 
            20                  25                  30          


Gly His Glu Gln Lys Lys Ala Asn Ile Tyr Lys Gly Lys Ile Thr Arg 
        35                  40                  45              


Ile Glu Pro Ser Leu Glu Ala Ala Phe Val Asp Tyr Gly Ala Glu Arg 
    50                  55                  60                  


His Gly Phe Leu Pro Leu Lys Glu Ile Ala Arg Glu Tyr Phe Pro Ala 
65                  70                  75                  80  


Asn Tyr Ser Ala His Gly Arg Pro Asn Ile Lys Asp Val Leu Arg Glu 
                85                  90                  95      


Gly Gln Glu Val Ile Val Gln Ile Asp Lys Glu Glu Arg Gly Asn Lys 
            100                 105                 110         


Gly Ala Ala Leu Thr Thr Phe Ile Ser Leu Ala Gly Ser Tyr Leu Val 
        115                 120                 125             


Leu Met Pro Asn Asn Pro Arg Ala Gly Gly Ile Ser Arg Arg Ile Glu 
    130                 135                 140                 


Gly Asp Asp Arg Thr Glu Leu Lys Glu Ala Leu Ala Ser Leu Glu Leu 
145                 150                 155                 160 


Pro Glu Gly Met Gly Leu Ile Val Arg Thr Ala Gly Val Gly Lys Ser 
                165                 170                 175     


Ala Glu Ala Leu Gln Trp Asp Leu Ser Phe Arg Leu Lys His Trp Glu 
            180                 185                 190         


Ala Ile Lys Lys Ala Ala Glu Ser Arg Pro Ala Pro Phe Leu Ile His 
        195                 200                 205             


Gln Glu Ser Asn Val Ile Val Arg Ala Phe Arg Asp Tyr Leu Arg Gln 
    210                 215                 220                 


Asp Ile Gly Glu Ile Leu Ile Asp Asn Pro Lys Val Leu Glu Leu Ala 
225                 230                 235                 240 


Arg Gln His Ile Ala Ala Leu Gly Arg Pro Asp Phe Ser Ser Lys Ile 
                245                 250                 255     


Lys Leu Tyr Thr Gly Glu Ile Pro Leu Phe Ser His Tyr Gln Ile Glu 
            260                 265                 270         


Ser Gln Ile Glu Ser Ala Phe Gln Arg Glu Val Arg Leu Pro Ser Gly 
        275                 280                 285             


Gly Ser Ile Val Ile Asp Ser Thr Glu Ala Leu Thr Ala Ile Asp Ile 
    290                 295                 300                 


Asn Ser Ala Arg Ala Thr Arg Gly Gly Asp Ile Glu Glu Thr Ala Phe 
305                 310                 315                 320 


Asn Thr Asn Leu Glu Ala Ala Asp Glu Ile Ala Arg Gln Leu Arg Leu 
                325                 330                 335     


Arg Asp Leu Gly Gly Leu Ile Val Ile Asp Phe Ile Asp Met Thr Pro 
            340                 345                 350         


Val Arg His Gln Arg Ala Val Glu Asn Arg Leu Arg Glu Ala Val Arg 
        355                 360                 365             


Gln Asp Arg Ala Arg Ile Gln Ile Ser His Ile Ser Arg Phe Gly Leu 
    370                 375                 380                 


Leu Glu Met Ser Arg Gln Arg Leu Ser Pro Ser Leu Gly Glu Ser Ser 
385                 390                 395                 400 


His His Val Cys Pro Arg Cys Ser Gly Thr Gly Thr Val Arg Asp Asn 
                405                 410                 415     


Glu Ser Leu Ser Leu Ser Ile Leu Arg Leu Ile Glu Glu Glu Ala Leu 
            420                 425                 430         


Lys Glu Asn Thr Gln Glu Val His Ala Ile Val Pro Val Pro Ile Ala 
        435                 440                 445             


Ser Tyr Leu Leu Asn Glu Lys Arg Ser Ala Val Asn Ala Ile Glu Thr 
    450                 455                 460                 


Arg Gln Asp Gly Val Arg Cys Val Ile Val Pro Asn Asp Gln Met Glu 
465                 470                 475                 480 


Thr Pro His Tyr His Val Leu Arg Val Arg Lys Gly Glu Glu Thr Pro 
                485                 490                 495     


Thr Leu Ser Tyr Met Leu Pro Lys Leu His Glu Glu Ala Met Ala Leu 
            500                 505                 510         


Pro Ser Glu Glu Glu Phe Ala Glu Arg Lys Arg Pro Glu Gln Pro Ala 
        515                 520                 525             


Leu Ala Thr Phe Ala Met Pro Asp Val Pro Pro Ala Pro Thr Pro Ala 
    530                 535                 540                 


Glu Pro Ala Ala Pro Val Val Ala Pro Ala Pro Lys Ser Ala Pro Ala 
545                 550                 555                 560 


Thr Pro Ala Ala Pro Ala Gln Pro Gly Leu Leu Ser Arg Phe Phe Gly 
                565                 570                 575     


Ala Leu Lys Ala Leu Phe Ser Gly Gly Glu Glu Thr Lys Pro Ser Glu 
            580                 585                 590         


Gln Pro Thr Pro Lys Ala Glu Ala Lys Pro Glu Arg Gln Gln Asp Arg 
        595                 600                 605             


Arg Lys Pro Arg Gln Asn Asn Arg Arg Asp Arg Asn Glu Arg Arg Asp 
    610                 615                 620                 


Thr Arg Ser Glu Arg Thr Glu Gly Ser Asp Asn Arg Glu Glu Asn Arg 
625                 630                 635                 640 


Arg Asn Arg Arg Gln Ala Gln Gln Gln Thr Ala Glu Thr Arg Glu Ser 
                645                 650                 655     


Arg Gln Gln Val Glu Val Thr Glu Lys Ala Arg Thr Thr Asp Glu Gln 
            660                 665                 670         


Gln Ala Pro Arg Arg Glu Arg Ser Arg Arg Arg Asn Asp Asp Lys Arg 
        675                 680                 685             


Gln Ala Gln Gln Glu Ala Lys Ala Leu Asn Val Glu Glu Gln Gly Asn 
    690                 695                 700                 


Asp Ser Asn Leu Leu Ile Val Phe Tyr Val Gln Ile Met Pro Asp Asp 
705                 710                 715                 720 


Phe Val Met Gln Leu His Arg Phe 
                725             


<210>  88
<211>  295
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(295)
<223>  araBAD promoter

<400>  88
aaaccaattg tccatattgc atcagacatt gccgtcactg cgtcttttac tggctcttct       60

cgctaaccaa accggtaacc ccgcttatta aaagcattct gtaacaaagc gggaccaaag      120

ccatgacaaa aacgcgtaac aaaagtgtct ataatcacgg cagaaaagtc cacattgatt      180

atttgcacgg cgtcacactt tgctatgcca tagcattttt atccataaga ttagcggatc      240

ctacctgacg ctttttatcg caactctcta ctgtttctcc atacccgttt ttttg           295


<210>  89
<211>  54
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(54)
<223>  Ptet promoter

<400>  89
ttgacaatta atcatccggc tcgtataatg gaattgtgag cggataacaa ttcc             54


<210>  90
<211>  82
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(82)
<223>  Ptet promoter

<400>  90
tcatgtttga cagcttatca tcgattagct ttaatgcggt agtttatcac agttaaattg       60

ctaacgcagt caggcaccgt gt                                                82


<210>  91
<211>  29
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(29)
<223>  Ptac promoter

<400>  91
ttgacaatta atcatcggct cgtataatg                                         29


<210>  92
<211>  51
<212>  DNA
<213>  synthetic


<220>
<221>  promoter
<222>  (1)..(51)
<223>  PL promoter

<400>  92
ggcggtgttg acataaatac cactggcggt gatactgagc acatcagcag g                51


