                         SEQUENCE LISTING

<110>  New England Biolabs, Inc.
 
<120>  Programmable Cleavage of Double-Stranded DNA

<130>  NEB-440

<150>  17/335,500
<151>  2021-06-01

<160>  94    

<170>  PatentIn version 3.5

<210>  1
<211>  929
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  RecB protein sequence (truncated RecB with deleted nuclease 
       domain)

<400>  1

Met Ser Asp Val Ala Glu Thr Leu Asp Pro Leu Arg Leu Pro Leu Gln 
1               5                   10                  15      


Gly Glu Arg Leu Ile Glu Ala Ser Ala Gly Thr Gly Lys Thr Phe Thr 
            20                  25                  30          


Ile Ala Ala Leu Tyr Leu Arg Leu Leu Leu Gly Leu Gly Gly Ser Ala 
        35                  40                  45              


Ala Phe Pro Arg Pro Leu Thr Val Glu Glu Leu Leu Val Val Thr Phe 
    50                  55                  60                  


Thr Glu Ala Ala Thr Ala Glu Leu Arg Gly Arg Ile Arg Ser Asn Ile 
65                  70                  75                  80  


His Glu Leu Arg Ile Ala Cys Leu Arg Glu Thr Thr Asp Asn Pro Leu 
                85                  90                  95      


Tyr Glu Arg Leu Leu Glu Glu Ile Asp Asp Lys Ala Gln Ala Ala Gln 
            100                 105                 110         


Trp Leu Leu Leu Ala Glu Arg Gln Met Asp Glu Ala Ala Val Phe Thr 
        115                 120                 125             


Ile His Gly Phe Cys Gln Arg Met Leu Asn Leu Asn Ala Phe Glu Ser 
    130                 135                 140                 


Gly Met Leu Phe Glu Gln Gln Leu Ile Glu Asp Glu Ser Leu Leu Arg 
145                 150                 155                 160 


Tyr Gln Ala Cys Ala Asp Phe Trp Arg Arg His Cys Tyr Pro Leu Pro 
                165                 170                 175     


Arg Glu Ile Ala Gln Val Val Phe Glu Thr Trp Lys Gly Pro Gln Ala 
            180                 185                 190         


Leu Leu Arg Asp Ile Asn Arg Tyr Leu Gln Gly Glu Ala Pro Val Ile 
        195                 200                 205             


Lys Ala Pro Pro Pro Asp Asp Glu Thr Leu Ala Ser Arg His Ala Gln 
    210                 215                 220                 


Ile Val Ala Arg Ile Asp Thr Val Lys Gln Gln Trp Arg Asp Ala Val 
225                 230                 235                 240 


Gly Glu Leu Asp Ala Leu Ile Glu Ser Ser Gly Ile Asp Arg Arg Lys 
                245                 250                 255     


Phe Asn Arg Ser Asn Gln Ala Lys Trp Ile Asp Lys Ile Ser Ala Trp 
            260                 265                 270         


Ala Glu Glu Glu Thr Asn Ser Tyr Gln Leu Pro Glu Ser Leu Glu Lys 
        275                 280                 285             


Phe Ser Gln Arg Phe Leu Glu Asp Arg Thr Lys Ala Gly Gly Glu Thr 
    290                 295                 300                 


Pro Arg His Pro Leu Phe Glu Ala Ile Asp Gln Leu Leu Ala Glu Pro 
305                 310                 315                 320 


Leu Ser Ile Arg Asp Leu Val Ile Thr Arg Ala Leu Ala Glu Ile Arg 
                325                 330                 335     


Glu Thr Val Ala Arg Glu Lys Arg Arg Arg Gly Glu Leu Gly Phe Asp 
            340                 345                 350         


Asp Met Leu Ser Arg Leu Asp Ser Ala Leu Arg Ser Glu Ser Gly Glu 
        355                 360                 365             


Val Leu Ala Ala Ala Ile Arg Thr Arg Phe Pro Val Ala Met Ile Asp 
    370                 375                 380                 


Glu Phe Gln Asp Thr Asp Pro Gln Gln Tyr Arg Ile Phe Arg Arg Ile 
385                 390                 395                 400 


Trp His His Gln Pro Glu Thr Ala Leu Leu Leu Ile Gly Asp Pro Lys 
                405                 410                 415     


Gln Ala Ile Tyr Ala Phe Arg Gly Ala Asp Ile Phe Thr Tyr Met Lys 
            420                 425                 430         


Ala Arg Ser Glu Val His Ala His Tyr Thr Leu Asp Thr Asn Trp Arg 
        435                 440                 445             


Ser Ala Pro Gly Met Val Asn Ser Val Asn Lys Leu Phe Ser Gln Thr 
    450                 455                 460                 


Asp Asp Ala Phe Met Phe Arg Glu Ile Pro Phe Ile Pro Val Lys Ser 
465                 470                 475                 480 


Ala Gly Lys Asn Gln Ala Leu Arg Phe Val Phe Lys Gly Glu Thr Gln 
                485                 490                 495     


Pro Ala Met Lys Met Trp Leu Met Glu Gly Glu Ser Cys Gly Val Gly 
            500                 505                 510         


Asp Tyr Gln Ser Thr Met Ala Gln Val Cys Ala Ala Gln Ile Arg Asp 
        515                 520                 525             


Trp Leu Gln Ala Gly Gln Arg Gly Glu Ala Leu Leu Met Asn Gly Asp 
    530                 535                 540                 


Asp Ala Arg Pro Val Arg Ala Ser Asp Ile Ser Val Leu Val Arg Ser 
545                 550                 555                 560 


Arg Gln Glu Ala Ala Gln Val Arg Asp Ala Leu Thr Leu Leu Glu Ile 
                565                 570                 575     


Pro Ser Val Tyr Leu Ser Asn Arg Asp Ser Val Phe Glu Thr Leu Glu 
            580                 585                 590         


Ala Gln Glu Met Leu Trp Leu Leu Gln Ala Val Met Thr Pro Glu Arg 
        595                 600                 605             


Glu Asn Thr Leu Arg Ser Ala Leu Ala Thr Ser Met Met Gly Leu Asn 
    610                 615                 620                 


Ala Leu Asp Ile Glu Thr Leu Asn Asn Asp Glu His Ala Trp Asp Val 
625                 630                 635                 640 


Val Val Glu Glu Phe Asp Gly Tyr Arg Gln Ile Trp Arg Lys Arg Gly 
                645                 650                 655     


Val Met Pro Met Leu Arg Ala Leu Met Ser Ala Arg Asn Ile Ala Glu 
            660                 665                 670         


Asn Leu Leu Ala Thr Ala Gly Gly Glu Arg Arg Leu Thr Asp Ile Leu 
        675                 680                 685             


His Ile Ser Glu Leu Leu Gln Glu Ala Gly Thr Gln Leu Glu Ser Glu 
    690                 695                 700                 


His Ala Leu Val Arg Trp Leu Ser Gln His Ile Leu Glu Pro Asp Ser 
705                 710                 715                 720 


Asn Ala Ser Ser Gln Gln Met Arg Leu Glu Ser Asp Lys His Leu Val 
                725                 730                 735     


Gln Ile Val Thr Ile His Lys Ser Lys Gly Leu Glu Tyr Pro Leu Val 
            740                 745                 750         


Trp Leu Pro Phe Ile Thr Asn Phe Arg Val Gln Glu Gln Ala Phe Tyr 
        755                 760                 765             


His Asp Arg His Ser Phe Glu Ala Val Leu Asp Leu Asn Ala Ala Pro 
    770                 775                 780                 


Glu Ser Val Asp Leu Ala Glu Ala Glu Arg Leu Ala Glu Asp Leu Arg 
785                 790                 795                 800 


Leu Leu Tyr Val Ala Leu Thr Arg Ser Val Trp His Cys Ser Leu Gly 
                805                 810                 815     


Val Ala Pro Leu Val Arg Arg Arg Gly Asp Lys Lys Gly Asp Thr Asp 
            820                 825                 830         


Val His Gln Ser Ala Leu Gly Arg Leu Leu Gln Lys Gly Glu Pro Gln 
        835                 840                 845             


Asp Ala Ala Gly Leu Arg Thr Cys Ile Glu Ala Leu Cys Asp Asp Asp 
    850                 855                 860                 


Ile Ala Trp Gln Thr Ala Gln Thr Gly Asp Asn Gln Pro Trp Gln Val 
865                 870                 875                 880 


Asn Asp Val Ser Thr Ala Glu Leu Asn Ala Lys Thr Leu Gln Arg Leu 
                885                 890                 895     


Pro Gly Asp Asn Trp Arg Val Thr Ser Tyr Ser Gly Leu Gln Gln Arg 
            900                 905                 910         


Gly His Gly Ile Ala Gln Asp Leu Met Pro Arg Leu Asp Val Asp Ala 
        915                 920                 925             


Ala 
    


<210>  2
<211>  1180
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  RecB exo- mutant protein sequence (mutant carries mutations 
       E1020A, D1080A and K1082A to abolish nuclease activity)

<400>  2

Met Ser Asp Val Ala Glu Thr Leu Asp Pro Leu Arg Leu Pro Leu Gln 
1               5                   10                  15      


Gly Glu Arg Leu Ile Glu Ala Ser Ala Gly Thr Gly Lys Thr Phe Thr 
            20                  25                  30          


Ile Ala Ala Leu Tyr Leu Arg Leu Leu Leu Gly Leu Gly Gly Ser Ala 
        35                  40                  45              


Ala Phe Pro Arg Pro Leu Thr Val Glu Glu Leu Leu Val Val Thr Phe 
    50                  55                  60                  


Thr Glu Ala Ala Thr Ala Glu Leu Arg Gly Arg Ile Arg Ser Asn Ile 
65                  70                  75                  80  


His Glu Leu Arg Ile Ala Cys Leu Arg Glu Thr Thr Asp Asn Pro Leu 
                85                  90                  95      


Tyr Glu Arg Leu Leu Glu Glu Ile Asp Asp Lys Ala Gln Ala Ala Gln 
            100                 105                 110         


Trp Leu Leu Leu Ala Glu Arg Gln Met Asp Glu Ala Ala Val Phe Thr 
        115                 120                 125             


Ile His Gly Phe Cys Gln Arg Met Leu Asn Leu Asn Ala Phe Glu Ser 
    130                 135                 140                 


Gly Met Leu Phe Glu Gln Gln Leu Ile Glu Asp Glu Ser Leu Leu Arg 
145                 150                 155                 160 


Tyr Gln Ala Cys Ala Asp Phe Trp Arg Arg His Cys Tyr Pro Leu Pro 
                165                 170                 175     


Arg Glu Ile Ala Gln Val Val Phe Glu Thr Trp Lys Gly Pro Gln Ala 
            180                 185                 190         


Leu Leu Arg Asp Ile Asn Arg Tyr Leu Gln Gly Glu Ala Pro Val Ile 
        195                 200                 205             


Lys Ala Pro Pro Pro Asp Asp Glu Thr Leu Ala Ser Arg His Ala Gln 
    210                 215                 220                 


Ile Val Ala Arg Ile Asp Thr Val Lys Gln Gln Trp Arg Asp Ala Val 
225                 230                 235                 240 


Gly Glu Leu Asp Ala Leu Ile Glu Ser Ser Gly Ile Asp Arg Arg Lys 
                245                 250                 255     


Phe Asn Arg Ser Asn Gln Ala Lys Trp Ile Asp Lys Ile Ser Ala Trp 
            260                 265                 270         


Ala Glu Glu Glu Thr Asn Ser Tyr Gln Leu Pro Glu Ser Leu Glu Lys 
        275                 280                 285             


Phe Ser Gln Arg Phe Leu Glu Asp Arg Thr Lys Ala Gly Gly Glu Thr 
    290                 295                 300                 


Pro Arg His Pro Leu Phe Glu Ala Ile Asp Gln Leu Leu Ala Glu Pro 
305                 310                 315                 320 


Leu Ser Ile Arg Asp Leu Val Ile Thr Arg Ala Leu Ala Glu Ile Arg 
                325                 330                 335     


Glu Thr Val Ala Arg Glu Lys Arg Arg Arg Gly Glu Leu Gly Phe Asp 
            340                 345                 350         


Asp Met Leu Ser Arg Leu Asp Ser Ala Leu Arg Ser Glu Ser Gly Glu 
        355                 360                 365             


Val Leu Ala Ala Ala Ile Arg Thr Arg Phe Pro Val Ala Met Ile Asp 
    370                 375                 380                 


Glu Phe Gln Asp Thr Asp Pro Gln Gln Tyr Arg Ile Phe Arg Arg Ile 
385                 390                 395                 400 


Trp His His Gln Pro Glu Thr Ala Leu Leu Leu Ile Gly Asp Pro Lys 
                405                 410                 415     


Gln Ala Ile Tyr Ala Phe Arg Gly Ala Asp Ile Phe Thr Tyr Met Lys 
            420                 425                 430         


Ala Arg Ser Glu Val His Ala His Tyr Thr Leu Asp Thr Asn Trp Arg 
        435                 440                 445             


Ser Ala Pro Gly Met Val Asn Ser Val Asn Lys Leu Phe Ser Gln Thr 
    450                 455                 460                 


Asp Asp Ala Phe Met Phe Arg Glu Ile Pro Phe Ile Pro Val Lys Ser 
465                 470                 475                 480 


Ala Gly Lys Asn Gln Ala Leu Arg Phe Val Phe Lys Gly Glu Thr Gln 
                485                 490                 495     


Pro Ala Met Lys Met Trp Leu Met Glu Gly Glu Ser Cys Gly Val Gly 
            500                 505                 510         


Asp Tyr Gln Ser Thr Met Ala Gln Val Cys Ala Ala Gln Ile Arg Asp 
        515                 520                 525             


Trp Leu Gln Ala Gly Gln Arg Gly Glu Ala Leu Leu Met Asn Gly Asp 
    530                 535                 540                 


Asp Ala Arg Pro Val Arg Ala Ser Asp Ile Ser Val Leu Val Arg Ser 
545                 550                 555                 560 


Arg Gln Glu Ala Ala Gln Val Arg Asp Ala Leu Thr Leu Leu Glu Ile 
                565                 570                 575     


Pro Ser Val Tyr Leu Ser Asn Arg Asp Ser Val Phe Glu Thr Leu Glu 
            580                 585                 590         


Ala Gln Glu Met Leu Trp Leu Leu Gln Ala Val Met Thr Pro Glu Arg 
        595                 600                 605             


Glu Asn Thr Leu Arg Ser Ala Leu Ala Thr Ser Met Met Gly Leu Asn 
    610                 615                 620                 


Ala Leu Asp Ile Glu Thr Leu Asn Asn Asp Glu His Ala Trp Asp Val 
625                 630                 635                 640 


Val Val Glu Glu Phe Asp Gly Tyr Arg Gln Ile Trp Arg Lys Arg Gly 
                645                 650                 655     


Val Met Pro Met Leu Arg Ala Leu Met Ser Ala Arg Asn Ile Ala Glu 
            660                 665                 670         


Asn Leu Leu Ala Thr Ala Gly Gly Glu Arg Arg Leu Thr Asp Ile Leu 
        675                 680                 685             


His Ile Ser Glu Leu Leu Gln Glu Ala Gly Thr Gln Leu Glu Ser Glu 
    690                 695                 700                 


His Ala Leu Val Arg Trp Leu Ser Gln His Ile Leu Glu Pro Asp Ser 
705                 710                 715                 720 


Asn Ala Ser Ser Gln Gln Met Arg Leu Glu Ser Asp Lys His Leu Val 
                725                 730                 735     


Gln Ile Val Thr Ile His Lys Ser Lys Gly Leu Glu Tyr Pro Leu Val 
            740                 745                 750         


Trp Leu Pro Phe Ile Thr Asn Phe Arg Val Gln Glu Gln Ala Phe Tyr 
        755                 760                 765             


His Asp Arg His Ser Phe Glu Ala Val Leu Asp Leu Asn Ala Ala Pro 
    770                 775                 780                 


Glu Ser Val Asp Leu Ala Glu Ala Glu Arg Leu Ala Glu Asp Leu Arg 
785                 790                 795                 800 


Leu Leu Tyr Val Ala Leu Thr Arg Ser Val Trp His Cys Ser Leu Gly 
                805                 810                 815     


Val Ala Pro Leu Val Arg Arg Arg Gly Asp Lys Lys Gly Asp Thr Asp 
            820                 825                 830         


Val His Gln Ser Ala Leu Gly Arg Leu Leu Gln Lys Gly Glu Pro Gln 
        835                 840                 845             


Asp Ala Ala Gly Leu Arg Thr Cys Ile Glu Ala Leu Cys Asp Asp Asp 
    850                 855                 860                 


Ile Ala Trp Gln Thr Ala Gln Thr Gly Asp Asn Gln Pro Trp Gln Val 
865                 870                 875                 880 


Asn Asp Val Ser Thr Ala Glu Leu Asn Ala Lys Thr Leu Gln Arg Leu 
                885                 890                 895     


Pro Gly Asp Asn Trp Arg Val Thr Ser Tyr Ser Gly Leu Gln Gln Arg 
            900                 905                 910         


Gly His Gly Ile Ala Gln Asp Leu Met Pro Arg Leu Asp Val Asp Ala 
        915                 920                 925             


Ala Gly Val Ala Ser Val Val Glu Glu Pro Thr Leu Thr Pro His Gln 
    930                 935                 940                 


Phe Pro Arg Gly Ala Ser Pro Gly Thr Phe Leu His Ser Leu Phe Glu 
945                 950                 955                 960 


Asp Leu Asp Phe Thr Gln Pro Val Asp Pro Asn Trp Val Arg Glu Lys 
                965                 970                 975     


Leu Glu Leu Gly Gly Phe Glu Ser Gln Trp Glu Pro Val Leu Thr Glu 
            980                 985                 990         


Trp Ile Thr Ala Val Leu Gln Ala  Pro Leu Asn Glu Thr  Gly Val Ser 
        995                 1000                 1005             


Leu Ser  Gln Leu Ser Ala Arg  Asn Lys Gln Val Ala  Met Glu Phe 
    1010                 1015                 1020             


Tyr Leu  Pro Ile Ser Glu Pro  Leu Ile Ala Ser Gln  Leu Asp Thr 
    1025                 1030                 1035             


Leu Ile  Arg Gln Phe Asp Pro  Leu Ser Ala Gly Cys  Pro Pro Leu 
    1040                 1045                 1050             


Glu Phe  Met Gln Val Arg Gly  Met Leu Lys Gly Phe  Ile Asp Leu 
    1055                 1060                 1065             


Val Phe  Arg His Glu Gly Arg  Tyr Tyr Leu Leu Ala  Tyr Ala Ser 
    1070                 1075                 1080             


Asn Trp  Leu Gly Glu Asp Ser  Ser Ala Tyr Thr Gln  Gln Ala Met 
    1085                 1090                 1095             


Ala Ala  Ala Met Gln Ala His  Arg Tyr Asp Leu Gln  Tyr Gln Leu 
    1100                 1105                 1110             


Tyr Thr  Leu Ala Leu His Arg  Tyr Leu Arg His Arg  Ile Ala Asp 
    1115                 1120                 1125             


Tyr Asp  Tyr Glu His His Phe  Gly Gly Val Ile Tyr  Leu Phe Leu 
    1130                 1135                 1140             


Arg Gly  Val Asp Lys Glu His  Pro Gln Gln Gly Ile  Tyr Thr Thr 
    1145                 1150                 1155             


Arg Pro  Asn Ala Gly Leu Ile  Ala Leu Met Asp Glu  Met Phe Ala 
    1160                 1165                 1170             


Gly Met  Thr Leu Glu Glu Ala  
    1175                 1180 


<210>  3
<211>  1122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  RecC protein sequence

<400>  3

Met Leu Arg Val Tyr His Ser Asn Arg Leu Asp Val Leu Glu Ala Leu 
1               5                   10                  15      


Met Glu Phe Ile Val Glu Arg Glu Arg Leu Asp Asp Pro Phe Glu Pro 
            20                  25                  30          


Glu Met Ile Leu Val Gln Ser Thr Gly Met Ala Gln Trp Leu Gln Met 
        35                  40                  45              


Thr Leu Ser Gln Lys Phe Gly Ile Ala Ala Asn Ile Asp Phe Pro Leu 
    50                  55                  60                  


Pro Ala Ser Phe Ile Trp Asp Met Phe Val Arg Val Leu Pro Glu Ile 
65                  70                  75                  80  


Pro Lys Glu Ser Ala Phe Asn Lys Gln Ser Met Ser Trp Lys Leu Met 
                85                  90                  95      


Thr Leu Leu Pro Gln Leu Leu Glu Arg Glu Asp Phe Thr Leu Leu Arg 
            100                 105                 110         


His Tyr Leu Thr Asp Asp Ser Asp Lys Arg Lys Leu Phe Gln Leu Ser 
        115                 120                 125             


Ser Lys Ala Ala Asp Leu Phe Asp Gln Tyr Leu Val Tyr Arg Pro Asp 
    130                 135                 140                 


Trp Leu Ala Gln Trp Glu Thr Gly His Leu Val Glu Gly Leu Gly Glu 
145                 150                 155                 160 


Ala Gln Ala Trp Gln Ala Pro Leu Trp Lys Ala Leu Val Glu Tyr Thr 
                165                 170                 175     


His Gln Leu Gly Gln Pro Arg Trp His Arg Ala Asn Leu Tyr Gln Arg 
            180                 185                 190         


Phe Ile Glu Thr Leu Glu Ser Ala Thr Thr Cys Pro Pro Gly Leu Pro 
        195                 200                 205             


Ser Arg Val Phe Ile Cys Gly Ile Ser Ala Leu Pro Pro Val Tyr Leu 
    210                 215                 220                 


Gln Ala Leu Gln Ala Leu Gly Lys His Ile Glu Ile His Leu Leu Phe 
225                 230                 235                 240 


Thr Asn Pro Cys Arg Tyr Tyr Trp Gly Asp Ile Lys Asp Pro Ala Tyr 
                245                 250                 255     


Leu Ala Lys Leu Leu Thr Arg Gln Arg Arg His Ser Phe Glu Asp Arg 
            260                 265                 270         


Glu Leu Pro Leu Phe Arg Asp Ser Glu Asn Ala Gly Gln Leu Phe Asn 
        275                 280                 285             


Ser Asp Gly Glu Gln Asp Val Gly Asn Pro Leu Leu Ala Ser Trp Gly 
    290                 295                 300                 


Lys Leu Gly Arg Asp Tyr Ile Tyr Leu Leu Ser Asp Leu Glu Ser Ser 
305                 310                 315                 320 


Gln Glu Leu Asp Ala Phe Val Asp Val Thr Pro Asp Asn Leu Leu His 
                325                 330                 335     


Asn Ile Gln Ser Asp Ile Leu Glu Leu Glu Asn Arg Ala Val Ala Gly 
            340                 345                 350         


Val Asn Ile Glu Glu Phe Ser Arg Ser Asp Asn Lys Arg Pro Leu Asp 
        355                 360                 365             


Pro Leu Asp Ser Ser Ile Thr Phe His Val Cys His Ser Pro Gln Arg 
    370                 375                 380                 


Glu Val Glu Val Leu His Asp Arg Leu Leu Ala Met Leu Glu Glu Asp 
385                 390                 395                 400 


Pro Thr Leu Thr Pro Arg Asp Ile Ile Val Met Val Ala Asp Ile Asp 
                405                 410                 415     


Ser Tyr Ser Pro Phe Ile Gln Ala Val Phe Gly Ser Ala Pro Ala Asp 
            420                 425                 430         


Arg Tyr Leu Pro Tyr Ala Ile Ser Asp Arg Arg Ala Arg Gln Ser His 
        435                 440                 445             


Pro Val Leu Glu Ala Phe Ile Ser Leu Leu Ser Leu Pro Asp Ser Arg 
    450                 455                 460                 


Phe Val Ser Glu Asp Val Leu Ala Leu Leu Asp Val Pro Val Leu Ala 
465                 470                 475                 480 


Ala Arg Phe Asp Ile Thr Glu Glu Gly Leu Arg Tyr Leu Arg Gln Trp 
                485                 490                 495     


Val Asn Glu Ser Gly Ile Arg Trp Gly Ile Asp Asp Asp Asn Val Arg 
            500                 505                 510         


Glu Leu Glu Leu Pro Ala Thr Gly Gln His Thr Trp Arg Phe Gly Leu 
        515                 520                 525             


Thr Arg Met Leu Leu Gly Tyr Ala Met Glu Ser Ala Gln Gly Glu Trp 
    530                 535                 540                 


Gln Ser Val Leu Pro Tyr Asp Glu Ser Ser Gly Leu Ile Ala Glu Leu 
545                 550                 555                 560 


Val Gly His Leu Ala Ser Leu Leu Met Gln Leu Asn Ile Trp Arg Arg 
                565                 570                 575     


Gly Leu Ala Gln Glu Arg Pro Leu Glu Glu Trp Leu Pro Val Cys Arg 
            580                 585                 590         


Asp Met Leu Asn Ala Phe Phe Leu Pro Asp Ala Glu Thr Glu Ala Ala 
        595                 600                 605             


Met Thr Leu Ile Glu Gln Gln Trp Gln Ala Ile Ile Ala Glu Gly Leu 
    610                 615                 620                 


Gly Ala Gln Tyr Gly Asp Ala Val Pro Leu Ser Leu Leu Arg Asp Glu 
625                 630                 635                 640 


Leu Ala Gln Arg Leu Asp Gln Glu Arg Ile Ser Gln Arg Phe Leu Ala 
                645                 650                 655     


Gly Pro Val Asn Ile Cys Thr Leu Met Pro Met Arg Ser Ile Pro Phe 
            660                 665                 670         


Lys Val Val Cys Leu Leu Gly Met Asn Asp Gly Val Tyr Pro Arg Gln 
        675                 680                 685             


Leu Ala Pro Leu Gly Phe Asp Leu Met Ser Gln Lys Pro Lys Arg Gly 
    690                 695                 700                 


Asp Arg Ser Arg Arg Asp Asp Asp Arg Tyr Leu Phe Leu Glu Ala Leu 
705                 710                 715                 720 


Ile Ser Ala Gln Gln Lys Leu Tyr Ile Ser Tyr Ile Gly Arg Ser Ile 
                725                 730                 735     


Gln Asp Asn Ser Glu Arg Phe Pro Ser Val Leu Val Gln Glu Leu Ile 
            740                 745                 750         


Asp Tyr Ile Gly Gln Ser His Tyr Leu Pro Gly Asp Glu Ala Leu Asn 
        755                 760                 765             


Cys Asp Glu Ser Glu Ala Arg Val Lys Ala His Leu Thr Cys Leu His 
    770                 775                 780                 


Thr Arg Met Pro Phe Asp Pro Gln Asn Tyr Gln Pro Gly Glu Arg Gln 
785                 790                 795                 800 


Ser Tyr Ala Arg Glu Trp Leu Pro Ala Ala Ser Gln Ala Gly Lys Ala 
                805                 810                 815     


His Ser Glu Phe Val Gln Pro Leu Pro Phe Thr Leu Pro Glu Thr Val 
            820                 825                 830         


Pro Leu Glu Thr Leu Gln Arg Phe Trp Ala His Pro Val Arg Ala Phe 
        835                 840                 845             


Phe Gln Met Arg Leu Gln Val Asn Phe Arg Thr Glu Asp Ser Glu Ile 
    850                 855                 860                 


Pro Asp Thr Glu Pro Phe Ile Leu Glu Gly Leu Ser Arg Tyr Gln Ile 
865                 870                 875                 880 


Asn Gln Gln Leu Leu Asn Ala Leu Val Glu Gln Asp Asp Ala Glu Arg 
                885                 890                 895     


Leu Phe Arg Arg Phe Arg Ala Ala Gly Asp Leu Pro Tyr Gly Ala Phe 
            900                 905                 910         


Gly Glu Ile Phe Trp Glu Thr Gln Cys Gln Glu Met Gln Gln Leu Ala 
        915                 920                 925             


Asp Arg Val Ile Ala Cys Arg Gln Pro Gly Gln Ser Met Glu Ile Asp 
    930                 935                 940                 


Leu Ala Cys Asn Gly Val Gln Ile Thr Gly Trp Leu Pro Gln Val Gln 
945                 950                 955                 960 


Pro Asp Gly Leu Leu Arg Trp Arg Pro Ser Leu Leu Ser Val Ala Gln 
                965                 970                 975     


Gly Met Gln Leu Trp Leu Glu His Leu Val Tyr Cys Ala Ser Gly Gly 
            980                 985                 990         


Asn Gly Glu Ser Arg Leu Phe Leu  Arg Lys Asp Gly Glu  Trp Arg Phe 
        995                 1000                 1005             


Pro Pro  Leu Ala Ala Glu Gln  Ala Leu His Tyr Leu  Ser Gln Leu 
    1010                 1015                 1020             


Ile Glu  Gly Tyr Arg Glu Gly  Met Ser Ala Pro Leu  Leu Val Leu 
    1025                 1030                 1035             


Pro Glu  Ser Gly Gly Ala Trp  Leu Lys Thr Cys Tyr  Asp Ala Gln 
    1040                 1045                 1050             


Asn Asp  Ala Met Leu Asp Asp  Asp Ser Thr Leu Gln  Lys Ala Arg 
    1055                 1060                 1065             


Thr Lys  Phe Leu Gln Ala Tyr  Glu Gly Asn Met Met  Val Arg Gly 
    1070                 1075                 1080             


Glu Gly  Asp Asp Ile Trp Tyr  Gln Arg Leu Trp Arg  Gln Leu Thr 
    1085                 1090                 1095             


Pro Glu  Thr Met Glu Ala Ile  Val Glu Gln Ser Gln  Arg Phe Leu 
    1100                 1105                 1110             


Leu Pro  Leu Phe Arg Phe Asn  Gln Ser 
    1115                 1120         


<210>  4
<211>  439
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Phage Slur07 Dda helicase sequence (YP_009197435.1)

<400>  4

Met Thr Phe Asp Asp Leu Thr Glu Gly Gln Lys Asn Ala Phe Asn Thr 
1               5                   10                  15      


Val Met Lys Ala Ile Lys Glu Lys Lys His His Val Thr Ile Asn Gly 
            20                  25                  30          


Pro Ala Gly Thr Gly Lys Thr Thr Leu Thr Lys Phe Ile Ile Glu Ala 
        35                  40                  45              


Leu Ile Ser Thr Gly Glu Thr Gly Ile Ile Leu Ala Ala Pro Thr His 
    50                  55                  60                  


Ala Ala Lys Lys Ile Leu Ser Lys Leu Ser Gly Lys Glu Ala Ser Thr 
65                  70                  75                  80  


Ile His Ser Ile Leu Lys Ile Asn Pro Val Thr Tyr Glu Glu Asn Val 
                85                  90                  95      


Leu Phe Glu Gln Lys Glu Val Pro Asp Leu Ala Lys Cys Arg Val Leu 
            100                 105                 110         


Ile Cys Asp Glu Val Ser Met Tyr Asp Arg Lys Leu Phe Lys Ile Leu 
        115                 120                 125             


Leu Ser Thr Ile Pro Pro Trp Cys Thr Ile Ile Gly Ile Gly Asp Asn 
    130                 135                 140                 


Lys Gln Ile Arg Pro Val Asp Pro Gly Glu Asn Thr Ala Tyr Ile Ser 
145                 150                 155                 160 


Pro Phe Phe Thr His Lys Asp Phe Tyr Gln Cys Glu Leu Thr Glu Val 
                165                 170                 175     


Lys Arg Ser Asn Ala Pro Ile Ile Asp Val Ala Thr Asp Val Arg Asn 
            180                 185                 190         


Gly Lys Trp Ile Tyr Asp Lys Val Val Asp Gly His Gly Val Arg Gly 
        195                 200                 205             


Phe Thr Gly Asp Thr Ala Leu Arg Asp Phe Met Val Asn Tyr Phe Ser 
    210                 215                 220                 


Ile Val Lys Ser Leu Asp Asp Leu Phe Glu Asn Arg Val Met Ala Phe 
225                 230                 235                 240 


Thr Asn Lys Ser Val Asp Lys Leu Asn Ser Ile Ile Arg Lys Lys Ile 
                245                 250                 255     


Phe Glu Thr Asp Lys Asp Phe Ile Val Gly Glu Ile Ile Val Met Gln 
            260                 265                 270         


Glu Pro Leu Ile Lys Thr Tyr Lys Ile Asp Gly Lys Pro Val Ser Glu 
        275                 280                 285             


Ile Ile Phe Asn Asn Gly Gln Leu Val Arg Ile Ile Glu Ala Glu Tyr 
    290                 295                 300                 


Thr Ser Thr Phe Val Lys Ala Arg Gly Val Pro Gly Glu Tyr Leu Ile 
305                 310                 315                 320 


Arg His Trp Asp Leu Thr Val Glu Thr Tyr Gly Asp Asp Glu Tyr Tyr 
                325                 330                 335     


Arg Glu Lys Ile Lys Ile Ile Ser Ser Asp Glu Glu Leu Tyr Lys Phe 
            340                 345                 350         


Asn Leu Phe Leu Gly Lys Thr Ala Glu Thr Tyr Lys Asn Trp Asn Lys 
        355                 360                 365             


Gly Gly Lys Ala Pro Trp Ser Asp Phe Trp Asp Ala Lys Ser Gln Phe 
    370                 375                 380                 


Ser Lys Val Lys Ala Leu Pro Ala Ser Thr Phe His Lys Ala Gln Gly 
385                 390                 395                 400 


Met Ser Val Asp Arg Ala Phe Ile Tyr Thr Pro Cys Ile His Tyr Ala 
                405                 410                 415     


Asp Ala Glu Leu Ala Gln Gln Leu Leu Tyr Val Gly Val Thr Arg Gly 
            420                 425                 430         


Arg Tyr Asp Val Phe Tyr Val 
        435                 


<210>  5
<211>  609
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  E.coli RecQ helicase sequence (WP_096324295.1)

<400>  5

Met Ala Gln Ala Glu Val Leu Asn Leu Glu Ser Gly Ala Lys Gln Val 
1               5                   10                  15      


Leu Gln Glu Thr Phe Gly Tyr Gln Gln Phe Arg Pro Gly Gln Glu Glu 
            20                  25                  30          


Ile Ile Asp Thr Val Leu Ser Gly Arg Asp Cys Leu Val Val Met Pro 
        35                  40                  45              


Thr Gly Gly Gly Lys Ser Leu Cys Tyr Gln Ile Pro Ala Leu Leu Leu 
    50                  55                  60                  


Asn Gly Leu Thr Val Val Val Ser Pro Leu Ile Ser Leu Met Lys Asp 
65                  70                  75                  80  


Gln Val Asp Gln Leu Gln Ala Asn Gly Val Ala Ala Ala Cys Leu Asn 
                85                  90                  95      


Ser Thr Gln Thr Arg Glu Gln Gln Leu Glu Val Met Thr Gly Cys Arg 
            100                 105                 110         


Thr Gly Gln Ile Arg Leu Leu Tyr Ile Ala Pro Glu Arg Leu Met Leu 
        115                 120                 125             


Asp Asn Phe Leu Glu His Leu Ala His Trp Asn Pro Val Leu Leu Ala 
    130                 135                 140                 


Val Asp Glu Ala His Cys Ile Ser Gln Trp Gly His Asp Phe Arg Pro 
145                 150                 155                 160 


Glu Tyr Ala Ala Leu Gly Gln Leu Arg Gln Arg Phe Pro Thr Leu Pro 
                165                 170                 175     


Phe Met Ala Leu Thr Ala Thr Ala Asp Asp Thr Thr Arg Gln Asp Ile 
            180                 185                 190         


Val Arg Leu Leu Gly Leu Asn Asp Pro Leu Ile Gln Ile Ser Ser Phe 
        195                 200                 205             


Asp Arg Pro Asn Ile Arg Tyr Met Leu Met Glu Lys Phe Lys Pro Leu 
    210                 215                 220                 


Asp Gln Leu Met Arg Tyr Val Gln Glu Gln Arg Gly Lys Ser Gly Ile 
225                 230                 235                 240 


Ile Tyr Cys Asn Ser Arg Ala Lys Val Glu Asp Thr Ala Ala Arg Leu 
                245                 250                 255     


Gln Ser Lys Gly Ile Ser Ala Ala Ala Tyr His Ala Gly Leu Glu Asn 
            260                 265                 270         


Asn Val Arg Ala Asp Val Gln Glu Lys Phe Gln Arg Asp Asp Leu Gln 
        275                 280                 285             


Ile Val Val Ala Thr Val Ala Phe Gly Met Gly Ile Asn Lys Pro Asn 
    290                 295                 300                 


Val Arg Phe Val Val His Phe Asp Ile Pro Arg Asn Ile Glu Ser Tyr 
305                 310                 315                 320 


Tyr Gln Glu Thr Gly Arg Ala Gly Arg Asp Gly Leu Pro Ala Glu Ala 
                325                 330                 335     


Met Leu Phe Tyr Asp Pro Ala Asp Met Ala Trp Leu Arg Arg Cys Leu 
            340                 345                 350         


Glu Glu Lys Pro Gln Gly Gln Leu Gln Asp Ile Glu Arg His Lys Leu 
        355                 360                 365             


Asn Ala Met Gly Ala Phe Ala Glu Ala Gln Thr Cys Arg Arg Leu Val 
    370                 375                 380                 


Leu Leu Asn Tyr Phe Gly Glu Gly Arg Gln Glu Pro Cys Gly Asn Cys 
385                 390                 395                 400 


Asp Ile Cys Leu Asp Pro Pro Lys Gln Tyr Asp Gly Ser Thr Asp Ala 
                405                 410                 415     


Gln Ile Ala Leu Ser Thr Ile Gly Arg Val Asn Gln Arg Phe Gly Met 
            420                 425                 430         


Gly Tyr Val Val Glu Val Ile Arg Gly Ala Asn Asn Gln Arg Ile Arg 
        435                 440                 445             


Asp Tyr Gly His Asp Lys Leu Lys Val Tyr Gly Met Gly Arg Asp Lys 
    450                 455                 460                 


Ser His Glu His Trp Val Ser Val Ile Arg Gln Leu Ile His Leu Gly 
465                 470                 475                 480 


Leu Val Thr Gln Asn Ile Ala Gln His Ser Ala Leu Gln Leu Thr Glu 
                485                 490                 495     


Ala Ala Arg Pro Val Leu Arg Gly Glu Ser Ser Leu Gln Leu Ala Val 
            500                 505                 510         


Pro Arg Ile Val Ala Leu Lys Pro Lys Ala Met Gln Lys Ser Phe Gly 
        515                 520                 525             


Gly Asn Tyr Asp Arg Lys Leu Phe Ala Lys Leu Arg Lys Leu Arg Lys 
    530                 535                 540                 


Ser Ile Ala Asp Glu Ser Asn Val Pro Pro Tyr Val Val Phe Asn Asp 
545                 550                 555                 560 


Ala Thr Leu Ile Glu Met Ala Glu Gln Met Pro Ile Thr Ala Ser Glu 
                565                 570                 575     


Met Leu Ser Val Asn Gly Val Gly Met Arg Lys Leu Glu Arg Phe Gly 
            580                 585                 590         


Lys Pro Phe Met Ala Leu Ile Arg Ala His Val Asp Gly Asp Asp Glu 
        595                 600                 605             


Glu 
    


<210>  6
<211>  724
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Clostridium perfringens Cpe RecQ DNA helicase sequence 
       (WP_011590145.1)

<400>  6

Met Lys Arg Ala Lys Glu Ile Leu Lys Ser Phe Tyr Gly Tyr Ser Glu 
1               5                   10                  15      


Phe Arg Lys Gly Gln Glu Glu Ile Ile Asn Glu Ile Ile Ser Gly Asn 
            20                  25                  30          


Asp Val Val Ala Ile Met Pro Thr Gly Gly Gly Lys Ser Ile Cys Tyr 
        35                  40                  45              


Gln Ile Pro Ala Leu Ile Leu Glu Gly Val Thr Val Val Ile Ser Pro 
    50                  55                  60                  


Leu Ile Ser Leu Met Lys Asp Gln Val Asp Ala Ile Asn Ser Met Gly 
65                  70                  75                  80  


Ile Glu Ser Ala Tyr Ile Asn Ser Thr Leu Ser Asn Glu Glu Ile Asn 
                85                  90                  95      


Asn Ile Phe Gln Ser Val Lys Asp Gly His Ile Lys Ile Leu Tyr Val 
            100                 105                 110         


Ala Pro Glu Arg Leu Glu Ser Met Glu Phe Leu Asn Leu Val Ser Asn 
        115                 120                 125             


Ile Asp Ile Ser Gln Val Ala Val Asp Glu Ala His Cys Val Ser Gln 
    130                 135                 140                 


Trp Gly His Asp Phe Arg Ser Ser Tyr Lys Phe Ile Gly Arg Phe Ile 
145                 150                 155                 160 


Asn Leu Leu Arg Lys Arg Pro Val Val Ser Ala Phe Thr Ala Thr Ala 
                165                 170                 175     


Thr Glu Glu Val Arg Lys Asp Ile Val Lys Leu Leu Glu Leu Asn Asn 
            180                 185                 190         


Pro Lys Val Phe Val Ser Gly Phe Asp Arg Asp Asn Leu Lys Ile Ile 
        195                 200                 205             


Ile Glu Lys Gly Val Asn Lys Ala His Tyr Ile Leu Asp Tyr Leu Lys 
    210                 215                 220                 


Glu Asn Lys Asp Val Ser Gly Ile Ile Tyr Cys Ala Thr Arg Lys Glu 
225                 230                 235                 240 


Val Asp Ser Leu Cys Glu Leu Ile Asn Ser Arg Gly Ile Gly Cys Thr 
                245                 250                 255     


Lys Tyr His Ala Gly Leu Ser Asp Glu Glu Arg Lys Lys Asn Gln Glu 
            260                 265                 270         


Asp Phe Val Phe Asp Lys Val Ser Val Met Val Ala Thr Asn Ala Phe 
        275                 280                 285             


Gly Met Gly Ile Asp Lys Pro Asn Ile Arg Tyr Val Ile His Asn Asn 
    290                 295                 300                 


Met Pro Lys Asn Ile Glu Gly Tyr Tyr Gln Glu Ile Gly Arg Ala Gly 
305                 310                 315                 320 


Arg Asp Gly Glu Lys Ser Glu Cys Ile Leu Ile Phe Ser Pro Gly Asp 
                325                 330                 335     


Val Gln Thr Gln Lys Tyr Ile Ile Glu Thr Gly Thr Leu Asn Pro Glu 
            340                 345                 350         


Arg Lys Ile Asn Glu Leu Ser Lys Leu Gln Thr Met Met Asp Leu Val 
        355                 360                 365             


Tyr Ser Asn Gly Cys Tyr Arg Arg Phe Ile Leu Asn Tyr Phe Gly Glu 
    370                 375                 380                 


Asp Leu Glu Glu Asp Cys His Asn Cys Ser Asn Cys Glu Met Glu Gly 
385                 390                 395                 400 


Glu Leu Val Asp Lys Thr Ile Asp Ala Gln Lys Val Ile Ser Cys Val 
                405                 410                 415     


Tyr Arg Met Lys Arg Pro Phe Gly Ile Gly Val Ile Ile Asp Val Leu 
            420                 425                 430         


Arg Ala Ser Lys Asn Lys Lys Ile Ile Glu Leu Gly Leu Asp Gln Leu 
        435                 440                 445             


Ser Thr Tyr Gly Ile Met Lys Asp Tyr Ser Lys Glu Gly Leu Lys Asp 
    450                 455                 460                 


Phe Ile Asn Thr Leu Ile Ser His Arg Tyr Ile Asp Tyr Ile Gly Glu 
465                 470                 475                 480 


Tyr Pro Val Val Arg Leu Asn Gln Ser Ser Ile Asp Ile Leu Lys Gly 
                485                 490                 495     


Asn Glu Lys Val Met Phe Lys Glu Lys Ile Lys Ala Lys Arg Val Ser 
            500                 505                 510         


Lys Asn Asn Glu Leu Phe Asp Leu Leu Arg Ser Leu Arg Arg Glu Ile 
        515                 520                 525             


Ala Leu Glu Glu Gly Val Pro Pro Tyr Ile Val Phe Gly Asp Asn Thr 
    530                 535                 540                 


Leu Lys Glu Met Ser Val Arg Ile Pro Val Asn Glu Ser Gln Leu Ser 
545                 550                 555                 560 


Glu Ile Ser Gly Val Gly Glu Lys Lys Ile Leu Lys Tyr Gly Glu Gln 
                565                 570                 575     


Phe Leu Asn Val Ile Lys Glu Tyr Val Lys Val Asn Asn Ile Glu Val 
            580                 585                 590         


Gln Trp Glu Ser Gly Leu Lys Ile Asn Lys Asp Glu Leu Glu Glu Lys 
        595                 600                 605             


Phe Lys Lys Gly Asn Gly Lys Asp Gly Lys Lys Lys Ser Phe Glu Ile 
    610                 615                 620                 


Thr Ile Asp Met Leu Arg Glu Gly Asp Lys Phe His Thr Ile Ala Lys 
625                 630                 635                 640 


Glu Arg Glu Leu Ser Ile Thr Thr Val Leu Ser His Ile Gln Gln Tyr 
                645                 650                 655     


Leu Glu Glu Gly Asn Lys Ile Asp Phe Glu Ile Asp Phe Asp Ser Leu 
            660                 665                 670         


Phe Ser Lys Arg Glu Glu Glu Leu Val Leu Asp Ala Ile Asp Lys Val 
        675                 680                 685             


Gly Tyr Ser Lys Leu Lys Pro Ile Lys Glu Asn Leu Glu Glu Asp Val 
    690                 695                 700                 


Ser Tyr Glu Lys Ile Arg Phe Ile Val Thr Lys Leu Thr Ile Asn Asn 
705                 710                 715                 720 


Leu Arg Lys Glu 
                


<210>  7
<211>  816
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  MISC_FEATURE
<223>  Clostridium butyricum Cbu RecQ DNA helicase sequence 
       (WP_003411240.1)

<400>  7

Met Ile Asn Lys Ser Pro Gln Ser Val Leu Glu Lys Tyr Tyr Gly Tyr 
1               5                   10                  15      


Lys Ser Phe Arg Glu Gly Gln Glu Asn Ile Ile Asn Ser Ile Val Gln 
            20                  25                  30          


Gly Arg Asp Val Leu Ala Ile Met Pro Thr Gly Gly Gly Lys Ser Ile 
        35                  40                  45              


Cys Tyr Gln Val Pro Ala Leu Met Leu Glu Gly Leu Thr Ile Val Ile 
    50                  55                  60                  


Ser Pro Leu Ile Ser Leu Met Lys Asp Gln Val Asp Thr Leu Lys Asp 
65                  70                  75                  80  


Met Gly Val Glu Ala Glu Tyr Ile Asn Ser Thr Leu Ser Ser Ala Glu 
                85                  90                  95      


Glu Asn Asp Ile Ile Asn Lys Ile Glu Arg Asn Glu Val Lys Ile Leu 
            100                 105                 110         


Tyr Ile Ala Pro Glu Arg Leu Ser Ser Ser Gly Phe Leu Asn Ile Ile 
        115                 120                 125             


Gly Arg Cys Ser Ile Ser Gln Ile Ala Val Asp Glu Ala His Cys Ile 
    130                 135                 140                 


Ser Gln Trp Gly His Asp Phe Arg Ser Ser Tyr Lys Lys Ile Ser His 
145                 150                 155                 160 


Phe Ile Gly Ile Leu Asn Lys Arg Pro Thr Val Thr Ala Phe Thr Ala 
                165                 170                 175     


Thr Ala Ser Glu Glu Val Arg Asp Asp Ile Ile Arg Leu Leu Lys Leu 
            180                 185                 190         


Glu Asn Pro Lys Ile Phe Ile Thr Gly Phe Asn Arg Glu Asn Leu Phe 
        195                 200                 205             


Ile Asn Ile Ile Lys Gly Gly Asp Lys Lys Asn Tyr Leu Leu Asp Tyr 
    210                 215                 220                 


Ile Asn Asn Asn Ser Asp Val Ser Gly Ile Ile Tyr Ala Ser Thr Arg 
225                 230                 235                 240 


Lys Glu Val Asp Asn Ile Tyr Glu Leu Leu Ser Ser Lys Gly Tyr Ser 
                245                 250                 255     


Val Val His Tyr His Ala Gly Leu Ser Glu Glu Ala Arg Arg Glu Asn 
            260                 265                 270         


Gln Glu Gly Phe Ile Tyr Asp Arg Ala Asn Ile Met Val Ala Thr Asn 
        275                 280                 285             


Ala Phe Gly Met Gly Ile Asp Lys Pro Asp Ile Arg Tyr Val Val His 
    290                 295                 300                 


Tyr Asn Met Pro Arg Asn Val Glu Ser Tyr Tyr Gln Glu Ile Gly Arg 
305                 310                 315                 320 


Ala Gly Arg Asp Gly Asp Lys Ser Glu Cys Ile Leu Leu Phe Ser Pro 
                325                 330                 335     


Gln Asp Val Gln Ile Gln Lys Tyr Leu Ile Glu Thr Ser Ile Glu Asn 
            340                 345                 350         


Pro Glu Arg Gln Asn Ile Gln Tyr Lys Lys Leu Gln Gln Met Ile Asp 
        355                 360                 365             


Phe Val Tyr Ser Asn Glu Cys Tyr Lys Lys Phe Ile Leu Ala Tyr Phe 
    370                 375                 380                 


Gly Glu Glu Leu Lys Asp Asp Cys Asn Ser Cys Ser Asn Cys Met Asn 
385                 390                 395                 400 


Glu Gly Glu Val Val Asp Lys Thr Val Asp Ala Gln Lys Val Leu Ser 
                405                 410                 415     


Cys Ile Tyr Arg Met Asn Gln Lys Phe Gly Ser Gly Met Val Val Asp 
            420                 425                 430         


Val Leu Arg Gly Ser Lys Asn Lys Lys Val Leu Gln Phe Lys Phe Asn 
        435                 440                 445             


Glu Leu Ser Thr Tyr Gly Ile Met Lys Glu Phe Ser Ala Asp Glu Leu 
    450                 455                 460                 


Lys Thr Phe Ile Asn Thr Leu Val Ser Gln Gly Tyr Ile Arg Val Val 
465                 470                 475                 480 


Glu Gly Thr Tyr Pro Ile Leu Ala Leu Asn Ser Met Ser Arg Lys Ile 
                485                 490                 495     


Leu Val Gly Glu Glu Lys Val Met Leu Lys Glu Phe Ala Ala His Lys 
            500                 505                 510         


Lys Val Arg Glu Asn Asn Glu Leu Phe Glu Ile Leu Lys Glu Leu Arg 
        515                 520                 525             


Lys Glu Ile Ala His Glu Asn Asn Val Pro Pro Tyr Val Ile Phe Gly 
    530                 535                 540                 


Asp Val Thr Leu Lys Glu Met Ser Val Lys Tyr Pro Val Tyr Lys Glu 
545                 550                 555                 560 


Ala Met Leu Asn Ile Thr Gly Val Gly Glu Leu Lys Tyr Ser Lys Tyr 
                565                 570                 575     


Gly Glu Leu Phe Glu Asn Thr Ile Arg Lys Tyr Ser Glu Asp Asn Asn 
            580                 585                 590         


Ile Lys Ile Ser Gly Glu Gly Thr Leu Glu Ile Ile Thr Ser Asn Asn 
        595                 600                 605             


Asp Asn Asp Leu Lys Leu Glu Ile Lys Thr Asp Asp Lys Leu Tyr Asn 
    610                 615                 620                 


Leu Leu Phe Glu Ala Arg Lys Lys Trp Ala Glu Lys Glu Lys Asn Ala 
625                 630                 635                 640 


Tyr Pro Gln Ser Ile Met His Met Asn Ile Leu Lys Glu Ile Ser Gly 
                645                 650                 655     


Arg Tyr Pro Val Ser Ile Glu Glu Leu Lys Asp Ile Ser Gly Phe Gly 
            660                 665                 670         


Pro Lys Lys Ile Glu Ala Tyr Gly Asp Glu Ile Ile Glu Ile Val Asn 
        675                 680                 685             


Asn Tyr Ile Leu Glu Asn Asn Ile Glu His Val Trp Ile Gln Lys Asn 
    690                 695                 700                 


Lys Arg Lys Val Ile Ile Asp Gly Glu Thr Arg Asn Ser Asp Gln Ile 
705                 710                 715                 720 


Ala Ile Asp Met Leu Lys Glu Asn Val Asn Ile His Glu Val Ser Glu 
                725                 730                 735     


Lys Leu Glu Leu Ser Val Ser Thr Ile Leu Gly Tyr Val Ser Glu Tyr 
            740                 745                 750         


Ile Lys Glu Phe Gly Glu Ile Lys Phe Asn Ile Asn Phe Glu Glu Phe 
        755                 760                 765             


Tyr Asn Glu Glu Glu Glu Ser Ala Ile Leu Asn Ala Ile Asn Glu Val 
    770                 775                 780                 


Gly Phe Glu Lys Ile Ser Asp Leu Lys Lys Ala Leu Pro Lys Tyr Thr 
785                 790                 795                 800 


Lys Tyr Glu Ser Ile Arg Ala Val Ile Leu Lys His Cys Ile Leu Ala 
                805                 810                 815     


<210>  8
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1 ss DNA target

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  8
tatttaaata atttaatata ctatacaacc tactacctca tataaatttt                  50


<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T-2 ss DNA target

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  9
cagaatggct tggactcaag tgtaaatgta cagggttcag ttttgtaata                  50


<210>  10
<211>  50
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T-3 ss RNA target

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  10
cagaauggcu uggacucaag uguaaaugua caggguucag uuuuguaaua                  50


<210>  11
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  ss-phiX174 ss DNA target

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  11
gtttgctgat gaactaagtc aacctcagca tcaaccttgc gagtcatttc ttt              53


<210>  12
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Top oligo, 60nt Anneals to 60 nt Bottom oligo

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  12
actcatgcag gcatgcagga acgcagtcag atttatgtgt catatagtac gtgattcaag       60


<210>  13
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom oligo, 60nt Anneals to 60nt Top oligo

<400>  13
taacgtattc aagatacctc gtactctgta ctgactgcgt tcctgcatgc ctgcatgagt       60


<210>  14
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Top oligo, 60 nt Anneals to Bottom 1-4 oligos

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  14
actcatgcag gcatgcagga tcgcagtcag atttatgtgt catatagtac gtgattcaag       60


<210>  15
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom olito 1, 60nt For Blunt stubstrate

<400>  15
cttgaatcac gtactatatg acacataaat ctgactgcga tcctgcatgc ctgcatgagt       60


<210>  16
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom oligo 2, 60 nt For forked substrate

<400>  16
taacgtattc gtactatatg acacataaat ctgactgcga tcctgcatgc ctgcatgagt       60


<210>  17
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom oligo 3, 50nt For substrate with 10 nt 3'-ss end

<400>  17
gtactatatg acacataaat ctgactgcga tcctgcatgc ctgcatgagt                  50


<210>  18
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom oligo 4, 70nt For substrate with 10 nt 5'-ss end

<400>  18
gtactctgta cttgaatcac gtactatatg acacataaat ctgactgcga tcctgcatgc       60

ctgcatgagt                                                              70


<210>  19
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  5'--FAM Top oligo, 80 nt Anneals to 80nt Bottom to make blunt end

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'--FAM

<400>  19
gctagataga ttgacagatt actcaagcag gcatgcagga tcgcagtcag atttatgtgt       60

catatagtac gtgattcaag                                                   80


<210>  20
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Botom oligo, 80 nt Anneals to 80 nt - FAM to make blunt substrate

<400>  20
cttgaatcac gtactatatg acacataaat ctgactgcga tcctgcatgc ctgcttgagt       60

aatctgtcaa tctatctagc                                                   80


<210>  21
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  5'-FAM Top oligo 1, 90 nt Anneals to 80 nt FAM Top to make 10 nt 
       5'-ss end

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  21
gctagataga ttgacagatt actcaagcag gcatgcagga tcgcagtcag atttatgtgt       60

catatagtac gtgattcaag tacagagaac                                        90


<210>  22
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom olito 1 90.nt Anneals to 90 nt Bottom 2 to make forked end

<400>  22
gttctctgta cttgaatcac gtactatatg acacataaat ctgactgcga tcctgcatgc       60

ctgcttgagt aatctgtcaa tctatctagc                                        90


<210>  23
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  5'-FAM Top oligo 2, 90 nt Anneals to 90 nt Bottom 2 to make 
       forked end

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  23
gctagataga ttgacagatt actcaagcag gcatgcagga tcgcagtcag atttatgtgt       60

catatagtac gtgattcaag tacagagaac                                        90


<210>  24
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom oligo 2, 90 nt Anneals to 90 nt FAM Top 2 to make forked 
       end

<400>  24
taaggtattc cttgaatcac gtactatatg acacataaat ctgactgcga tcctgcatgc       60

ctgcttgagt aatctgtcaa tctatctagc                                        90


<210>  25
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B12 DNA Guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  25
tgactgcgat cctgcat                                                      17


<210>  26
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  17 nt guide DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  26
tgactgcgtt cctgcat                                                      17


<210>  27
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  G-1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  27
tgaggtagta ggttgtatag t                                                 21


<210>  28
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  G-2 DNA Guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  28
tgaaccctgt acattta                                                      17


<210>  29
<211>  17
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  G-3 RNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  29
ugaacccugu acauuua                                                      17


<210>  30
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  30
tcgcaaggtt agtgctgagg t                                                 21


<210>  31
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-C1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  31
cgcaaggtta gtgctgaggt t                                                 21


<210>  32
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-G1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  32
gcaaggttag tgctgaggtt g                                                 21


<210>  33
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-C2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  33
caaggttagt gctgaggttg a                                                 21


<210>  34
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-A1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  34
aaggttagtg ctgaggttga c                                                 21


<210>  35
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-A2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  35
aggttagtgc tgaggttgac t                                                 21


<210>  36
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-G2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  36
ggttagtgct gaggttgact t                                                 21


<210>  37
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T1-G3 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  37
gttagtgctg aggttgactt a                                                 21


<210>  38
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  38
ttagtgctga ggttgactta g                                                 21


<210>  39
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T2-T2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  39
tagtgctgag gttgacttag t                                                 21


<210>  40
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T2-A1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  40
agtgctgagg ttgacttagt t                                                 21


<210>  41
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T2-G1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  41
gtgctgaggt tgacttagtt c                                                 21


<210>  42
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T3 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  42
tgctgaggtt gacttagttc a                                                 21


<210>  43
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B1 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  43
tgaactaagt caacctcagc a                                                 21


<210>  44
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B2 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  44
taagtcaacc tcagcactaa c                                                 21


<210>  45
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B3 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  45
tcaacctcag cactaacctt g                                                 21


<210>  46
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B4 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  46
tcagcactaa ccttgcgagt c                                                 21


<210>  47
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  XhoT DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  47
tttggtctaa ctttaccgct a                                                 21


<210>  48
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  XhoB DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  48
ttagtagcgg taaagttaga c                                                 21


<210>  49
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Sap21F DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  49
tggtcagatt ggtcgtctta t                                                 21


<210>  50
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  SapB DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  50
taagacgacc aatctgacca g                                                 21


<210>  51
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  StuT DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  51
tgatggaact gaccaaacgt c                                                 21


<210>  52
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  StuB DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  52
taacgacgtt tggtcagttc c                                                 21


<210>  53
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  AdB-450T DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  53
tcaagcaagt gtcttgctgt c                                                 21


<210>  54
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  AdB-450B DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  54
taaagacagc aagacacttg c                                                 21


<210>  55
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  AdA-20860T DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  55
tgtcatcttt taagcttgcc t                                                 21


<210>  56
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  AdA-20860B DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  56
taccaggcaa gcttaaaaga t                                                 21


<210>  57
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  T5 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  57
taggtatctc agttcggtgt a                                                 21


<210>  58
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B5 DNA guide

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  58
aacgacctac accgaactga g                                                 21


<210>  59
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  239-Fw PCR primer for 239 bp substrate, 25 nt

<400>  59
gccaaatgct tactcaagct caaac                                             25


<210>  60
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  239-ROX-Fw PCR primer for 239bp substrate, 25 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  60
gccaaatgct tactcaagct caaac                                             25


<210>  61
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  239-FAM-Rv PCR primer for 239bp substrate, 24 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  61
tccaagtatc ggcaacagct ttat                                              24


<210>  62
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  619-Fw PCR primer for 619bp substrate, 30 nt

<400>  62
ctaaaatgca actggacaat cagaaagaga                                        30


<210>  63
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  619-ROX-Fw PCR primer for 619bp substrate, 30 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  63
ctaaaatgca actggacaat cagaaagaga                                        30


<210>  64
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  619-FAM-Rv PCR primer for 619bp substrate, 29 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  64
tgacattcag aagggtaata agaacgaac                                         29


<210>  65
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  322-Fw PCR primer for 322bp substrate, 23 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  65
cagaaggagt ctactgctcg cgt                                               23


<210>  66
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  322-Rv PCR primer for 322bp substrate, 24 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  66
tccaagtatc ggcaacagct ttat                                              24


<210>  67
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  phiX174-ROX-T1-5 PCR primer for 332bp DNA1, 23nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  67
atgactcgca aggttagtgc tga                                               23


<210>  68
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  phiX174--FAM-Rv PCR primer for 332bp DNA1, 29 nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  68
tgacattcag aagggtaata agaacgaac                                         29


<210>  69
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  pUC19-300-Fw PCR primer for 300bp DNA2, 25nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  69
acatgtgagc aaaaggccag caaaa                                             25


<210>  70
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  pUC19-300-Rv PCR primer for 300bp DNA2, 23nt

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  70
agcgaacgac ctacaccgaa ctg                                               23


<210>  71
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  pET28c-Fw4 PCR primer for pET28c fragment, 22 nt

<400>  71
tgagatccgg ctgctaacaa ag                                                22


<210>  72
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  pET28c-Rv PCR primer for pET28c fragment, 21 nt

<400>  72
catatggctg ccgcgcggca c                                                 21


<210>  73
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B1-nhis-Fw PCR primer for recB B1 fragment,34 nt

<400>  73
gcgcggcagc catatgagtg atgtcgccga gaca                                   34


<210>  74
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B1-A1020-Rv PCR primer for recB B1 fragment, 27 nt

<400>  74
aactccatcg ccacctgttt attgcgg                                           27


<210>  75
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B2-A1020-Fw PCR primer for recB B2 fragment, 29 nt

<400>  75
acaggtggcg atggagtttt atctgccga                                         29


<210>  76
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B2-A1080-Fw PCR primer for recB B2 fragment, 32 nt

<400>  76
tgctcgccta tgcatccaac tggttgggtg aa                                     32


<210>  77
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B3-A1080-Rv PCR primer for recB B3 fragment, 33 nt

<400>  77
ttggatgcat aggcgagcag gtaataacgc cct                                    33


<210>  78
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  B3-end-Rv PCR primer for recB B3 fragment, 39 nt

<400>  78
gttagcagcc ggatctcatt acgcctcctc cagggtcat                              39


<210>  79
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Top strand, 60 nt Anneals to bottom strand

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<220>
<221>  misc_feature
<222>  (20)..(20)
<223>  m6A

<400>  79
actcatgcag gcatgcagga tcgcagtcag atttatgtgt catatagtac gtgattcaag       60


<210>  80
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom strand, 60 nt Anneals to top strand

<400>  80
taacgtattc aagatacctc gtactctgta ctgactgcga tcctgcatgc ctgcatgagt       60


<210>  81
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Trap oligo, 30 nt Anneals to top strand

<220>
<221>  misc_feature
<222>  (10)..(10)
<223>  m6A

<400>  81
ctgactgcga tcctgcatgc ctgcatgagt                                        30


<210>  82
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Top linker oligo 37 nt Anneals to bottom linker oligo

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos

<400>  82
gtaggtcgtt cgctatgact cgcaaggtta gtgctga                                37


<210>  83
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<223>  Bottom linker oligo, 37 nt Anneals to top linker oligo

<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/phos/

<400>  83
aaccttgcga gtcatagcga acgacctaca ccgaact                                37


<210>  84
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  84
aaagaaatga ctcgcaaggt tagtgctgag gttgacttag ttcatcagca aac              53


<210>  85
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  85
aaagaaatga ctcgcaaggt tagtgctgag gttgacttag ttcatcagca aac              53


<210>  86
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  86
gactcgcaag gttagtgctg aggttgactt agttcatca                              39


<210>  87
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  87
tgatgaacta agtcaacctc agcactaacc ttgcgagtc                              39


<210>  88
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  88
aaggttagtg ctgaggttga cttagtt                                           27


<210>  89
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  89
aactaagtca acctcagcac taacctt                                           27


<210>  90
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/ROX/

<400>  90
gcaaggttag tgctgaggtt gacttagtt                                         29


<210>  91
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  91
aactaagtca acctcagcac taaccttgc                                         29


<210>  92
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  92
caaggttagt gctgaggttg acttagttca                                        30


<210>  93
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'/FAM/

<400>  93
tgaactaagt caacctcagc actaaccttg                                        30


<210>  94
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic construct

<400>  94
cgtaattcgt acgaattacg                                                   20


