﻿                         序列表

<110>  江苏丰华生物制药有限公司

<120>  一种人源化抗人GPVI单克隆抗体Fab片段及其应用

<160>  17

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  600
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  1
Gln Ser Gly Pro Leu Pro Lys Pro Ser Leu Gln Ala Leu Pro Ser Ser 
1               5                   10                  15      
Leu Val Pro Leu Glu Lys Pro Val Thr Leu Arg Cys Gln Gly Pro Pro 
            20                  25                  30          
Gly Val Asp Leu Tyr Arg Leu Glu Lys Leu Ser Ser Ser Arg Tyr Gln 
        35                  40                  45              
Asp Gln Ala Val Leu Phe Ile Pro Ala Met Lys Arg Ser Leu Ala Gly 
    50                  55                  60                  
Arg Tyr Arg Cys Ser Tyr Gln Asn Gly Ser Leu Trp Ser Leu Pro Ser 
65                  70                  75                  80  
Asp Gln Leu Glu Leu Val Ala Thr Gly Val Phe Ala Lys Pro Ser Leu 
                85                  90                  95      
Ser Ala Gln Pro Gly Pro Ala Val Ser Ser Gly Gly Asp Val Thr Leu 
            100                 105                 110         
Gln Cys Gln Thr Arg Tyr Gly Phe Asp Gln Phe Ala Leu Tyr Lys Glu 
        115                 120                 125             
Gly Asp Pro Ala Pro Tyr Lys Asn Pro Glu Arg Trp Tyr Arg Ala Ser 
    130                 135                 140                 
Phe Pro Ile Ile Thr Val Thr Ala Ala His Ser Gly Thr Tyr Arg Cys 
145                 150                 155                 160 
Tyr Ser Phe Ser Ser Arg Asp Pro Tyr Leu Trp Ser Ala Pro Ser Asp 
                165                 170                 175     
Pro Leu Glu Leu Val Val Thr Gly Thr Ser Val Thr Pro Ser Arg Leu 
            180                 185                 190         
Pro Thr Glu Pro Pro Ser Pro Val Ala Glu Phe Ser Glu Ala Thr Ala 
        195                 200                 205             
Glu Leu Thr Val Ser Phe Thr Asn Glu Val Phe Thr Thr Glu Thr Ser 
    210                 215                 220                 
Arg Ser Ile Thr Ala Ser Pro Lys Glu Ser Asp Ser Pro Ala Gly Glu 
225                 230                 235                 240 
Ser Cys Pro Pro Val Leu His Gln Gly Gln Pro Gly Pro Asp Met Pro 
                245                 250                 255     
Arg Gly Cys Asp Pro Asn Asn Pro Gly Gly Val Ser Gly Arg Gly Leu 
            260                 265                 270         
Ala Gln Pro Glu Glu Ala Pro Ala Ala Gln Gly Gln Gly Cys Ala Glu 
        275                 280                 285             
Ala Ala Ser Ala Pro Pro Ala Pro Pro Ala Asp Pro Glu Ile Lys Arg 
    290                 295                 300                 
Gly Ser Gly Trp Arg Pro Thr Gly Cys Ser Gln Pro Arg Val Met Phe 
305                 310                 315                 320 
Met Thr Ala Glu Pro Gln Ala Arg Ser Tyr Pro Arg Glu Gly Ser Trp 
                325                 330                 335     
His Gly Arg Arg Leu Lys Asp Trp Arg Val Trp Ser Val Glu Ala Gly 
            340                 345                 350         
Gly Gln Arg Leu Gln Leu Trp Lys Arg Gly His Ala Ala Ser Ser Trp 
        355                 360                 365             
Cys Ser Ile Arg Glu Pro Phe Gly Gln Cys Leu Ser Val Cys Leu Pro 
    370                 375                 380                 
Leu Cys Leu Arg Ala Pro Ser Ile Trp Asp Gly Arg Asn Leu Trp Arg 
385                 390                 395                 400 
Pro His Pro Pro Pro Cys Thr Leu Trp Met Thr Trp Tyr Pro Gly Trp 
                405                 410                 415     
Thr Thr Tyr Trp Pro Leu Ser Ser Thr Ser Leu Ile Trp Ala Pro Asp 
            420                 425                 430         
Gly Ser Leu Arg Phe Pro Ala Leu Arg Val Asp Ser Val Pro Ser Ser 
        435                 440                 445             
Val Gln Asn Pro Pro Val Leu Pro Phe Gly Pro Leu Cys Ser Cys Leu 
    450                 455                 460                 
Val Phe Pro Arg Asn Ser His Pro His Ser Ile Ser His Cys Gly Leu 
465                 470                 475                 480 
Thr Asn Leu Leu Ser Ser Leu Arg Thr Gly Leu Ala Gly Ser Leu Gly 
                485                 490                 495     
Met Ser Phe Ile Phe Leu Ser Val Lys Leu Ala Arg Cys Pro Leu Pro 
            500                 505                 510         
Phe Thr Leu Glu Asn Lys Ile Ser Leu Cys Asn Met Val Lys Pro His 
        515                 520                 525             
Leu Tyr Gln Gln Asn Lys Lys Thr Gln Lys Leu Ala Arg Cys Gly Gly 
    530                 535                 540                 
Ala Ser Leu Tyr Ser Gln Gln Leu Arg Gly Leu Arg Trp Glu Asn Gly 
545                 550                 555                 560 
Leu Ser Leu Gly Gly Arg Gly Cys Ser Glu Leu Arg Ser His His Cys 
                565                 570                 575     
Thr Leu Ala Arg Val Thr Lys Pro Asp Phe Val Ser Lys Asn Thr Gly 
            580                 585                 590         
Met Asn Met Ser Ile Thr Leu Ile 
        595                 600 

<210>  2
<211>  378
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
gaggtgaagc tgcaggagtc agggggaggc ttagtgcagc ctggaaggtc cttgaaactc        60
tcctgtacag cctcaggatt cactttcagt gactatttca tgtcctgggt ccgccaggct       120
ccaacgcgtg gtctggagtg ggtcgcatcc attagttctg gtggtgctag cgcttactgg       180
cgagactccg tgaagggccg attcactatc tccagagata atgcaaaaag cgccctatac       240
ctgcaaatgg acagtctgag gtctgaggac acggccactt atttctgtgc aagaggggag       300
ctcgactttg attactgggg ccaaggagtc atggtcgcag tctcctctgc tgaaacgaca       360
cccccatctg tctatccg                                                     378

<210>  3
<211>  126
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  3
Glu Val Lys Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Leu Lys Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Phe Met Ser Trp Val Arg Gln Ala Pro Thr Arg Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Ser Ser Gly Gly Ala Ser Ala Tyr Trp Arg Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Ala Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      
Ala Arg Gly Glu Leu Asp Phe Asp Tyr Trp Gly Gln Gly Val Met Val 
            100                 105                 110         
Ala Val Ser Ser Ala Glu Thr Thr Pro Pro Ser Val Tyr Pro 
        115                 120                 125     

<210>  4
<211>  342
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
gctgacccaa actccactct cctgtctgca tctgtgggag acagagtcac tctcaactgc        60
acagcaagtc agaatgttta taagaactta gcctggtatc agcaaaagct tggagaagct       120
cccagactcc tgctttatag tgccaacagt ttgcaaacgg gcatcccatc acggttcagt       180
ggcagtggat ctggtccaga tttcacactc accatcagca gcctgcagcc tgaagatgtt       240
gcctcatatt tctgccagca gtattatagc gggaacacgt ttggagctgg gaccaagctg       300
gaactcaaac gggctgatgc tgcaccaact gtatctatct tc                          342

<210>  5
<211>  114
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  5
Ala Asp Pro Asn Ser Thr Leu Leu Ser Ala Ser Val Gly Asp Arg Val 
1               5                   10                  15      
Thr Leu Asn Cys Thr Ala Ser Gln Asn Val Tyr Lys Asn Leu Ala Trp 
            20                  25                  30          
Tyr Gln Gln Lys Leu Gly Glu Ala Pro Arg Leu Leu Leu Tyr Ser Ala 
        35                  40                  45              
Asn Ser Leu Gln Thr Gly Ile Pro Ser Arg Phe Ser Gly Ser Gly Ser 
    50                  55                  60                  
Gly Pro Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro Glu Asp Val 
65                  70                  75                  80  
Ala Ser Tyr Phe Cys Gln Gln Tyr Tyr Ser Gly Asn Thr Phe Gly Ala 
                85                  90                  95      
Gly Thr Lys Leu Glu Leu Lys Arg Ala Asp Ala Ala Pro Thr Val Ser 
            100                 105                 110         
Ile Phe 
        

<210>  6
<211>  13
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  6
Thr Ala Ser Gly Phe Thr Phe Ser Asp Tyr Phe Met Ser 
1               5                   10              

<210>  7
<211>  20
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  7
Trp Val Ala Ser Ile Ser Ser Gly Gly Ala Ser Ala Tyr Trp Arg Asp 
1               5                   10                  15      
Ser Val Lys Gly 
            20  

<210>  8
<211>  9
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  8
Ala Arg Gly Glu Leu Asp Phe Asp Tyr 
1               5                   

<210>  9
<211>  13
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  9
Thr Ala Ser Gln Asn Val Tyr Lys Asn Leu Ala Trp Tyr 
1               5                   10              

<210>  10
<211>  11
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  10
Leu Leu Leu Tyr Ser Ala Asn Ser Leu Gln Thr 
1               5                   10      

<210>  11
<211>  8
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  11
Gln Gln Tyr Tyr Ser Gly Asn Thr 
1               5               

<210>  12
<211>  116
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  12
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Phe Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Ser Ser Gly Gly Ala Ser Ala Tyr Trp Arg Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Glu Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser 
        115     

<210>  13
<211>  106
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  13
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Thr Ala Ser Gln Asn Val Tyr Lys Asn 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Leu 
        35                  40                  45              
Tyr Ser Ala Asn Ser Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Gly Asn Thr 
                85                  90                  95      
Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys 
            100                 105     

<210>  14
<211>  223
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  14
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Phe Thr Phe Ser Asp Tyr 
            20                  25                  30          
Phe Met Ser Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Ser Ile Ser Ser Gly Gly Ala Ser Ala Tyr Trp Arg Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn Ser Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Gly Glu Leu Asp Phe Asp Tyr Trp Gly Gln Gly Thr Leu Val 
            100                 105                 110         
Thr Val Ser Ser Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala 
        115                 120                 125             
Pro Ser Ser Lys Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu 
    130                 135                 140                 
Val Lys Asp Tyr Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly 
145                 150                 155                 160 
Ala Leu Thr Ser Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser 
                165                 170                 175     
Gly Leu Tyr Ser Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu 
            180                 185                 190         
Gly Thr Gln Thr Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr 
        195                 200                 205             
Lys Val Asp Lys Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His 
    210                 215                 220             

<210>  15
<211>  213
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  15
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Thr Ala Ser Gln Asn Val Tyr Lys Asn 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Leu 
        35                  40                  45              
Tyr Ser Ala Asn Ser Leu Gln Thr Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Tyr Ser Gly Asn Thr 
                85                  90                  95      
Phe Gly Gln Gly Thr Lys Leu Glu Ile Lys Arg Thr Val Ala Ala Pro 
            100                 105                 110         
Ser Val Phe Ile Phe Pro Pro Ser Asp Glu Gln Leu Lys Ser Gly Thr 
        115                 120                 125             
Ala Ser Val Val Cys Leu Leu Asn Asn Phe Tyr Pro Arg Glu Ala Lys 
    130                 135                 140                 
Val Gln Trp Lys Val Asp Asn Ala Leu Gln Ser Gly Asn Ser Gln Glu 
145                 150                 155                 160 
Ser Val Thr Glu Gln Asp Ser Lys Asp Ser Thr Tyr Ser Leu Ser Ser 
                165                 170                 175     
Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu Lys His Lys Val Tyr Ala 
            180                 185                 190         
Cys Glu Val Thr His Gln Gly Leu Ser Ser Pro Val Thr Lys Ser Phe 
        195                 200                 205             
Asn Arg Gly Glu Cys 
    210             

<210>  16
<211>  669
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  16
gaggtgcagc tggtggaatc tggaggcggc ctggtgcagc ctggaggctc tctgagactg        60
tcttgcacag ctagcggatt cacattttct gattatttta tgtcttgggt gagacaggcc       120
cctggaaagg gactggaatg ggtggcttct atctcttctg gaggagctag cgcttactgg       180
agagattctg tgaagggaag gtttactatt tctagagata acgctaagaa tagtctgtat       240
ctgcagatga atagtctgag agctgaagat acagctgtgt attattgtgc tagaggagag       300
ctggatttcg attactgggg ccagggcacc ctggtgaccg tgagctctgc ctctacaaag       360
ggccctagcg tgttccctct ggccccttct tctaagtcta ccagcggcgg caccgccgcc       420
ctgggctgtc tggtgaagga ctacttccca gagcctgtga ccgtgagctg gaacagcggc       480
gccctgacca gcggcgtgca caccttccct gccgtgctgc agagctctgg cctgtacagc       540
ctgtctagcg tggtgaccgt gcctagctct agcctgggca cccagaccta catctgtaac       600
gtgaaccaca agccatctaa tactaaagtg gataaaaagg tggaacctaa gagctgtgac       660
aaaacccac                                                               669

<210>  17
<211>  639
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
gatatccaga tgacccagtc tccttcttct ctgtctgcct ctgtgggcga tagagtgacc        60
atcacctgta ccgcctctca gaacgtgtac aaaaacctgg cctggtacca gcagaagcct       120
ggcaaggccc ctaagctgct gctgtactct gctaactctc tgcagacagg cgtgccttct       180
agattctctg gcagcggctc tggcaccgat ttcaccctga ccatctctag cctgcagcct       240
gaggatttcg ccacctacta ctgtcagcag tactactctg gcaacacctt cggccagggc       300
accaagctgg agatcaagag aaccgtggcc gccccttctg tgttcatctt ccctccttct       360
gatgagcagc tgaaatctgg caccgcctct gtggtgtgtc tgctgaacaa cttctaccct       420
agagaggcca aggtgcagtg gaaggtggat aacgccctgc agtctggcaa ctctcaggag       480
tctgtgaccg agcaggattc taaggatagc acctactctc tgagctctac attgacactg       540
agtaaggctg attatgagaa gcataaggtc tacgcctgtg aagtgaccca tcagggcctg       600
tctagccctg tgaccaagag cttcaacagg ggcgagtgc                              639

