                         SEQUENCE LISTING

<110>  BioNTech Cell & Gene Therapies GmbH
 
<120>  AGENTS AND METHODS FOR ACTIVATION AND TARGETING OF IMMUNE 
       EFFECTOR CELLS

<130>  674-381 PCT3

<150>  PCT/EP2021/065290
<151>  2021-06-08

<160>  51    

<170>  PatentIn version 3.5

<210>  1
<211>  47
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  5'-UTR

<400>  1
aacuaguauu cuucuggucc ccacagacuc agagagaacc cgccacc                     47


<210>  2
<211>  311
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR

<400>  2
cucgagagcu cgcuuucuug cuguccaauu ucuauuaaag guuccuuugu ucccuaaguc       60

caacuacuaa acugggggau auuaugaagg gccuugagca ucuggauucu gccuaauaaa      120

aaacauuuau uuucauugcu gcgucgagag cucgcuuucu ugcuguccaa uuucuauuaa      180

agguuccuuu guucccuaag uccaacuacu aaacuggggg auauuaugaa gggccuugag      240

caucuggauu cugccuaaua aaaaacauuu auuuucauug cugcgucgag accuggucca      300

gagucgcuag c                                                           311


<210>  3
<211>  278
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  3'-UTR

<400>  3
cugguacugc augcacgcaa ugcuagcugc cccuuucccg uccuggguac cccgagucuc       60

ccccgaccuc gggucccagg uaugcuccca ccuccaccug ccccacucac caccucugcu      120

aguuccagac accucccaag cacgcagcaa ugcagcucaa aacgcuuagc cuagccacac      180

ccccacggga aacagcagug auuaaccuuu agcaauaaac gaaaguuuaa cuaagcuaua      240

cuaaccccag gguuggucaa uuucgugcca gccacacc                              278


<210>  4
<211>  110
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  A30L70

<400>  4
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa gcauaugacu aaaaaaaaaa aaaaaaaaaa       60

aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa                 110


<210>  5
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Epitope tag

<400>  5

Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr Glu 
1               5                   10              


<210>  6
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR1

<400>  6

Gly Val Thr Ile Ser Ala Leu Asn Ala Met Ala Met Gly 
1               5                   10              


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR2

<400>  7

Ala Val Ser Glu Arg Gly Asn Ala Met 
1               5                   


<210>  8
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CDR3

<400>  8

Leu Glu Asp Arg Val Asp Ser Phe His Asp Tyr 
1               5                   10      


<210>  9
<211>  122
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VHH

<400>  9

Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Val Thr Ile Ser Ala Leu 
            20                  25                  30          


Asn Ala Met Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly Glu Arg Arg 
        35                  40                  45              


Val Met Val Ala Ala Val Ser Glu Arg Gly Asn Ala Met Tyr Arg Glu 
    50                  55                  60                  


Ser Val Gln Gly Arg Phe Thr Val Thr Arg Asp Phe Thr Asn Lys Met 
65                  70                  75                  80  


Val Ser Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys His Val Leu Glu Asp Arg Val Asp Ser Phe His Asp Tyr Trp 
            100                 105                 110         


Gly Gln Gly Thr Gln Val Thr Val Ser Ser 
        115                 120         


<210>  10
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG-Leader

<400>  10

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser 
            


<210>  11
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ALFA

<400>  11

Pro Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr Glu Pro 
1               5                   10                  15  


<210>  12
<211>  69
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD8 hinge

<400>  12

Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala 
1               5                   10                  15      


Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly 
            20                  25                  30          


Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile 
        35                  40                  45              


Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val 
    50                  55                  60                  


Ile Thr Leu Tyr Cys 
65                  


<210>  13
<211>  42
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human 41-BB domain

<400>  13

Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met 
1               5                   10                  15      


Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe 
            20                  25                  30          


Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu 
        35                  40          


<210>  14
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human CD3 zeta domain

<400>  14

Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly 
1               5                   10                  15      


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              


Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys 
    50                  55                  60                  


Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg 
65                  70                  75                  80  


Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala 
                85                  90                  95      


Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
            100                 105                 110         


<210>  15
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  15

Gly Gly Gly Gly Ser 
1               5   


<210>  16
<211>  254
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNFL9 (full sequence)

<400>  16

Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu 
                245                 250                 


<210>  17
<211>  26
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Human sec signal

<400>  17

Met Arg Val Met Ala Pro Arg Thr Leu Ile Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      


Leu Ala Leu Thr Glu Thr Trp Ala Gly Ser 
            20                  25      


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  18

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15  


<210>  19
<211>  37
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GPI Anchor (human CD55)

<400>  19

Pro Asn Lys Gly Ser Gly Thr Thr Ser Gly Thr Thr Arg Leu Leu Ser 
1               5                   10                  15      


Gly His Thr Cys Phe Thr Leu Thr Gly Leu Leu Gly Thr Leu Val Thr 
            20                  25                  30          


Met Gly Leu Leu Thr 
        35          


<210>  20
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  IgG-Leader

<400>  20

Met Asp Trp Thr Trp Arg Val Phe Cys Leu Leu Ala Val Ala Pro Gly 
1               5                   10                  15      


Ala His Ser 
            


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  21

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  22
<211>  117
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aCLDN6 VH

<400>  22

Glu Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Met Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Thr Met Asn Trp Val Lys Gln Ser His Gly Lys Asn Leu Glu Trp Ile 
        35                  40                  45              


Gly Leu Ile Asn Pro Tyr Asn Gly Gly Thr Ile Tyr Asn Gln Lys Phe 
    50                  55                  60                  


Lys Gly Lys Ala Thr Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu Leu Ser Leu Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys 
                85                  90                  95      


Ala Arg Asp Tyr Gly Phe Val Leu Asp Tyr Trp Gly Gln Gly Thr Thr 
            100                 105                 110         


Leu Thr Val Ser Ser 
        115         


<210>  23
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  aCLDN6 VL

<400>  23

Asp Ile Val Leu Thr Gln Ser Pro Ser Ile Met Ser Val Ser Pro Gly 
1               5                   10                  15      


Glu Lys Val Thr Ile Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met 
            20                  25                  30          


His Trp Phe Gln Gln Lys Pro Gly Thr Ser Pro Lys Leu Ser Ile Tyr 
        35                  40                  45              


Ser Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Arg 
    50                  55                  60                  


Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser Arg Val Ala Ala Glu 
65                  70                  75                  80  


Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Arg Ser Asn Tyr Pro Pro Trp 
                85                  90                  95      


Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro Ala 
            100                 105                 110         


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  24

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  25
<211>  3
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  25

Gly Gly Ser 
1           


<210>  26
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  His-Tag

<400>  26

His His His His His His 
1               5       


<210>  27
<211>  257
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  27

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Pro Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr 
            20                  25                  30          


Glu Pro Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
        35                  40                  45              


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
    50                  55                  60                  


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
65                  70                  75                  80  


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
                85                  90                  95      


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
            100                 105                 110         


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
        115                 120                 125             


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
    130                 135                 140                 


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln 
145                 150                 155                 160 


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
                165                 170                 175     


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
            180                 185                 190         


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        195                 200                 205             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    210                 215                 220                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
225                 230                 235                 240 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                245                 250                 255     


Arg 
    


<210>  28
<211>  262
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  28

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Pro Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr 
            20                  25                  30          


Glu Pro Gly Gly Gly Gly Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
        35                  40                  45              


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
    50                  55                  60                  


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
65                  70                  75                  80  


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
                85                  90                  95      


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
            100                 105                 110         


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
        115                 120                 125             


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
    130                 135                 140                 


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
145                 150                 155                 160 


Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
                165                 170                 175     


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
            180                 185                 190         


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
        195                 200                 205             


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
    210                 215                 220                 


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
225                 230                 235                 240 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
                245                 250                 255     


Gln Ala Leu Pro Pro Arg 
            260         


<210>  29
<211>  364
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  29

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Val Thr Ile 
        35                  40                  45              


Ser Ala Leu Asn Ala Met Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly 
    50                  55                  60                  


Glu Arg Arg Val Met Val Ala Ala Val Ser Glu Arg Gly Asn Ala Met 
65                  70                  75                  80  


Tyr Arg Glu Ser Val Gln Gly Arg Phe Thr Val Thr Arg Asp Phe Thr 
                85                  90                  95      


Asn Lys Met Val Ser Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys His Val Leu Glu Asp Arg Val Asp Ser Phe His 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Thr Thr Thr 
    130                 135                 140                 


Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro 
145                 150                 155                 160 


Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala Ala Gly Gly Ala Val 
                165                 170                 175     


His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro 
            180                 185                 190         


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
        195                 200                 205             


Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro 
    210                 215                 220                 


Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys 
225                 230                 235                 240 


Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe 
                245                 250                 255     


Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu 
            260                 265                 270         


Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp 
        275                 280                 285             


Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys 
    290                 295                 300                 


Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala 
305                 310                 315                 320 


Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys 
                325                 330                 335     


Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr 
            340                 345                 350         


Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg 
        355                 360                 


<210>  30
<211>  369
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CAR construct

<400>  30

Met Asp Trp Ile Trp Arg Ile Leu Phe Leu Val Gly Ala Ala Thr Gly 
1               5                   10                  15      


Ala His Ser Glu Val Gln Leu Gln Glu Ser Gly Gly Gly Leu Val Gln 
            20                  25                  30          


Pro Gly Gly Ser Leu Arg Leu Ser Cys Thr Ala Ser Gly Val Thr Ile 
        35                  40                  45              


Ser Ala Leu Asn Ala Met Ala Met Gly Trp Tyr Arg Gln Ala Pro Gly 
    50                  55                  60                  


Glu Arg Arg Val Met Val Ala Ala Val Ser Glu Arg Gly Asn Ala Met 
65                  70                  75                  80  


Tyr Arg Glu Ser Val Gln Gly Arg Phe Thr Val Thr Arg Asp Phe Thr 
                85                  90                  95      


Asn Lys Met Val Ser Leu Gln Met Asp Asn Leu Lys Pro Glu Asp Thr 
            100                 105                 110         


Ala Val Tyr Tyr Cys His Val Leu Glu Asp Arg Val Asp Ser Phe His 
        115                 120                 125             


Asp Tyr Trp Gly Gln Gly Thr Gln Val Thr Val Ser Ser Gly Gly Gly 
    130                 135                 140                 


Gly Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro Thr Pro Ala Pro Thr 
145                 150                 155                 160 


Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu Ala Cys Arg Pro Ala 
                165                 170                 175     


Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp Phe Ala Cys Asp Ile 
            180                 185                 190         


Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser 
        195                 200                 205             


Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg Lys Lys Leu Leu Tyr 
    210                 215                 220                 


Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu 
225                 230                 235                 240 


Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu 
                245                 250                 255     


Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Lys Gln 
            260                 265                 270         


Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu 
        275                 280                 285             


Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly 
    290                 295                 300                 


Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
305                 310                 315                 320 


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
                325                 330                 335     


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
            340                 345                 350         


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
        355                 360                 365             


Arg 
    


<210>  31
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Activation compound

<400>  31

Met Glu Tyr Ala Ser Asp Ala Ser Leu Asp Pro Glu Ala Pro Trp Pro 
1               5                   10                  15      


Pro Ala Pro Arg Ala Arg Ala Cys Arg Val Leu Pro Trp Ala Leu Val 
            20                  25                  30          


Ala Gly Leu Leu Leu Leu Leu Leu Leu Ala Ala Ala Cys Ala Val Phe 
        35                  40                  45              


Leu Ala Cys Pro Trp Ala Val Ser Gly Ala Arg Ala Ser Pro Gly Ser 
    50                  55                  60                  


Ala Ala Ser Pro Arg Leu Arg Glu Gly Pro Glu Leu Ser Pro Asp Asp 
65                  70                  75                  80  


Pro Ala Gly Leu Leu Asp Leu Arg Gln Gly Met Phe Ala Gln Leu Val 
                85                  90                  95      


Ala Gln Asn Val Leu Leu Ile Asp Gly Pro Leu Ser Trp Tyr Ser Asp 
            100                 105                 110         


Pro Gly Leu Ala Gly Val Ser Leu Thr Gly Gly Leu Ser Tyr Lys Glu 
        115                 120                 125             


Asp Thr Lys Glu Leu Val Val Ala Lys Ala Gly Val Tyr Tyr Val Phe 
    130                 135                 140                 


Phe Gln Leu Glu Leu Arg Arg Val Val Ala Gly Glu Gly Ser Gly Ser 
145                 150                 155                 160 


Val Ser Leu Ala Leu His Leu Gln Pro Leu Arg Ser Ala Ala Gly Ala 
                165                 170                 175     


Ala Ala Leu Ala Leu Thr Val Asp Leu Pro Pro Ala Ser Ser Glu Ala 
            180                 185                 190         


Arg Asn Ser Ala Phe Gly Phe Gln Gly Arg Leu Leu His Leu Ser Ala 
        195                 200                 205             


Gly Gln Arg Leu Gly Val His Leu His Thr Glu Ala Arg Ala Arg His 
    210                 215                 220                 


Ala Trp Gln Leu Thr Gln Gly Ala Thr Val Leu Gly Leu Phe Arg Val 
225                 230                 235                 240 


Thr Pro Glu Ile Pro Ala Gly Leu Pro Ser Pro Arg Ser Glu Pro Ser 
                245                 250                 255     


Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr Glu 
            260                 265             


<210>  32
<211>  92
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Activation compound

<400>  32

Met Arg Val Met Ala Pro Arg Thr Leu Ile Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      


Leu Ala Leu Thr Glu Thr Trp Ala Gly Ser Ser Arg Leu Glu Glu Glu 
            20                  25                  30          


Leu Arg Arg Arg Leu Thr Glu Pro Glu Ala Ala Ala Lys Glu Ala Ala 
        35                  40                  45              


Ala Lys Glu Ala Ala Ala Lys Pro Asn Lys Gly Ser Gly Thr Thr Ser 
    50                  55                  60                  


Gly Thr Thr Arg Leu Leu Ser Gly His Thr Cys Phe Thr Leu Thr Gly 
65                  70                  75                  80  


Leu Leu Gly Thr Leu Val Thr Met Gly Leu Leu Thr 
                85                  90          


<210>  33
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Activation compound

<400>  33

Met Arg Val Met Ala Pro Arg Thr Leu Ile Leu Leu Leu Ser Gly Ala 
1               5                   10                  15      


Leu Ala Leu Thr Glu Thr Trp Ala Gly Ser Glu Val Gln Leu Gln Glu 
            20                  25                  30          


Ser Gly Gly Gly Leu Val Gln Pro Gly Gly Ser Leu Arg Leu Ser Cys 
        35                  40                  45              


Thr Ala Ser Gly Val Thr Ile Ser Ala Leu Asn Ala Met Ala Met Gly 
    50                  55                  60                  


Trp Tyr Arg Gln Ala Pro Gly Glu Arg Arg Val Met Val Ala Ala Val 
65                  70                  75                  80  


Ser Glu Arg Gly Asn Ala Met Tyr Arg Glu Ser Val Gln Gly Arg Phe 
                85                  90                  95      


Thr Val Thr Arg Asp Phe Thr Asn Lys Met Val Ser Leu Gln Met Asp 
            100                 105                 110         


Asn Leu Lys Pro Glu Asp Thr Ala Val Tyr Tyr Cys His Val Leu Glu 
        115                 120                 125             


Asp Arg Val Asp Ser Phe His Asp Tyr Trp Gly Gln Gly Thr Gln Val 
    130                 135                 140                 


Thr Val Ser Ser Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala 
145                 150                 155                 160 


Ala Ala Lys Pro Asn Lys Gly Ser Gly Thr Thr Ser Gly Thr Thr Arg 
                165                 170                 175     


Leu Leu Ser Gly His Thr Cys Phe Thr Leu Thr Gly Leu Leu Gly Thr 
            180                 185                 190         


Leu Val Thr Met Gly Leu Leu Thr 
        195                 200 


<210>  34
<211>  292
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Docketing compound

<400>  34

Met Asp Trp Thr Trp Arg Val Phe Cys Leu Leu Ala Val Ala Pro Gly 
1               5                   10                  15      


Ala His Ser Pro Ser Arg Leu Glu Glu Glu Leu Arg Arg Arg Leu Thr 
            20                  25                  30          


Glu Pro Gly Gly Gly Ser Gly Gly Gly Ser Glu Val Gln Leu Gln Gln 
        35                  40                  45              


Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser Met Lys Ile Ser Cys 
    50                  55                  60                  


Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Thr Met Asn Trp Val Lys 
65                  70                  75                  80  


Gln Ser His Gly Lys Asn Leu Glu Trp Ile Gly Leu Ile Asn Pro Tyr 
                85                  90                  95      


Asn Gly Gly Thr Ile Tyr Asn Gln Lys Phe Lys Gly Lys Ala Thr Leu 
            100                 105                 110         


Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met Glu Leu Leu Ser Leu 
        115                 120                 125             


Thr Ser Glu Asp Ser Ala Val Tyr Tyr Cys Ala Arg Asp Tyr Gly Phe 
    130                 135                 140                 


Val Leu Asp Tyr Trp Gly Gln Gly Thr Thr Leu Thr Val Ser Ser Gly 
145                 150                 155                 160 


Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Asp Ile Val Leu Thr 
                165                 170                 175     


Gln Ser Pro Ser Ile Met Ser Val Ser Pro Gly Glu Lys Val Thr Ile 
            180                 185                 190         


Thr Cys Ser Ala Ser Ser Ser Val Ser Tyr Met His Trp Phe Gln Gln 
        195                 200                 205             


Lys Pro Gly Thr Ser Pro Lys Leu Ser Ile Tyr Ser Thr Ser Asn Leu 
    210                 215                 220                 


Ala Ser Gly Val Pro Ala Arg Phe Ser Gly Arg Gly Ser Gly Thr Ser 
225                 230                 235                 240 


Tyr Ser Leu Thr Ile Ser Arg Val Ala Ala Glu Asp Ala Ala Thr Tyr 
                245                 250                 255     


Tyr Cys Gln Gln Arg Ser Asn Tyr Pro Pro Trp Thr Phe Gly Gly Gly 
            260                 265                 270         


Thr Lys Leu Glu Ile Lys Arg Ser Asp Pro Ala Gly Gly Ser His His 
        275                 280                 285             


His His His His 
    290         


<210>  35
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG-Leader

<400>  35
atggactgga tctggcgaat actgtttctg gtgggagccg ccactggggc ccattct          57


<210>  36
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  ALFA

<400>  36
cctagcaggc tggaggagga actccgcaga cggctgaccg aaccc                       45


<210>  37
<211>  207
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human CD8 hinge

<400>  37
actactaccc cagcacctag accgcctaca cccgcaccca ctatcgcgtc tcagcccttg       60

agtctgcggc ccgaggcttg tcggcccgca gctggcgggg ctgtgcatac ccgaggactc      120

gactttgcat gcgacatcta catttgggcc cctctggccg gcacttgcgg cgtccttctt      180

ctgagtctgg tcataacgtt gtattgc                                          207


<210>  38
<211>  126
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human 41-BB domain

<400>  38
aaacggggca ggaagaaact gctgtatatc ttcaagcagc ctttcatgcg cccagttcag       60

accactcagg aggaggatgg gtgttcctgt cgtttccctg aggaagaaga aggcgggtgc      120

gaattg                                                                 126


<210>  39
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human CD3 zeta domain

<400>  39
agggtcaagt ttagccgatc agctgacgcc cctgcttaca aacaggggca aaatcagctt       60

tacaatgagc tgaacctcgg gcgtagagag gagtacgacg tgttggacaa gcgcagaggg      120

agagatcccg agatgggcgg caaaccaaga cgcaagaatc cccaagaagg cctctacaac      180

gagctgcaga aggataaaat ggccgaagcc tatagcgaga ttggcatgaa aggagaacgg      240

aggagaggga aaggtcacga tggactctac caaggcctga gcacagctac caaagacacg      300

tatgatgcct tgcacatgca agcactgcca cccaggtag                             339


<210>  40
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  40
ggaggaggcg ggagc                                                        15


<210>  41
<211>  762
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  TNFL9 (full sequence)

<400>  41
atggagtatg ctagcgatgc ctctctggac ccggaagctc catggccacc agctcctagg       60

gctcgcgctt gccgtgtgct tccttgggcc ctggtggctg gcctcctgtt gctgctgctg      120

ctggctgccg catgcgctgt gttcctcgcc tgtccatggg cggtaagtgg cgcgagagcc      180

tctcctggtt cagccgcatc accgaggctg agggaagggc cagagcttag ccccgatgac      240

cctgctgggt tgctcgacct gagacagggg atgtttgccc agttggtagc gcagaacgtg      300

ctgctgatcg acggtccctt gagctggtat tccgatcccg gtcttgccgg agtcagcctc      360

actggcggcc tgagttacaa ggaggacacc aaggaactgg tggttgccaa agccggtgtc      420

tactacgtgt tcttccagct cgaactcagg cgcgtggttg caggagaggg gtcaggctct      480

gtgagtcttg cccttcatct ccagcccctg agaagcgcag ccggagccgc tgcactggcg      540

ctgaccgtgg atctcccacc cgcctcctcc gaagcccgca atagcgcttt tggcttccaa      600

gggcgactgt tgcacctgtc tgcaggccaa cggctgggag tccatctgca cacggaggcc      660

agagcacgac acgcatggca gctgacacag ggagccactg tcctgggact gtttcgggtt      720

acacccgaga ttcctgcagg ccttccctcc cctcggtccg ag                         762


<210>  42
<211>  78
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Human sec signal

<400>  42
atgagagtga tggcccccag aaccctgatc ctgctgctgt ctggcgccct ggccctgaca       60

gagacatggg ccggaagc                                                     78


<210>  43
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  43
gaggcggcag ctaaggaagc tgctgctaaa gaggctgcgg ctaag                       45


<210>  44
<211>  114
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  GPI Anchor (human CD55)

<400>  44
ccaaataaag gaagtggaac cacttcaggt actacccgtc ttctatctgg gcacacgtgt       60

ttcacgttga caggtttgct tgggacgcta gtaaccatgg gcttgctgac ttga            114


<210>  45
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  IgG-Leader

<400>  45
atggattgga cttggcgggt cttttgcctg ttagcagtgg cccccggcgc tcatagc          57


<210>  46
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  46
ggaggaggcg ggagcggcgg gggtagt                                           27


<210>  47
<211>  351
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aCLDN6 VH

<400>  47
gaagttcagc tgcagcagtc agggccggaa ctggtgaaac ccggcgctag catgaagatc       60

tcctgtaagg cgagcgggta ttcctttacc ggctacacca tgaactgggt taagcaatct      120

cacggcaaga accttgagtg gataggactc attaatcctt acaatggcgg cacaatctac      180

aaccagaagt tcaaaggcaa agcaaccctt accgtggaca agagcagctc tacagcctat      240

atggagctgc tctcactgac gtccgaggat agcgcagtgt actattgtgc gcgggattac      300

gggtttgtgc tggattattg gggacagggc accacactga ccgtaagcag t               351


<210>  48
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aCLDN6 VL

<400>  48
gacattgtcc tgacacagag tccatccatt atgagcgtga gtcctggtga aaaggttaca       60

atcacctgca gtgcaagctc atcagtgtct tacatgcatt ggtttcagca gaagccggga      120

acttctccta agctgagcat ctactctacg agcaatctcg cctctggtgt cccagcgagg      180

ttctcaggac gcgggtccgg gacttcctat tccctcacaa tttccagggt agccgctgaa      240

gatgctgcca cctattattg ccaacagcgc agcaactacc caccctggac attcggtggt      300

ggaacaaaac tggagattaa gcggtccgac ccagcc                                336


<210>  49
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  49
ggaggcggtg gaagtggcgg tggagggtca ggaggtggtg gatct                       45


<210>  50
<211>  9
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  50
ggcggctct                                                                9


<210>  51
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  His-Tag

<400>  51
caccaccacc accatcat                                                     18


