                         SEQUENCE LISTING

<110>  University of Cape Town
 
<120>  Tobamovirus Pseudovirions for Stabilising Single Stranded RNA

<130>  PA176629/PCT

<150>  UK 2108392.8
<151>  2021-06-11

<160>  11    

<170>  PatentIn version 3.5

<210>  1
<211>  1234
<212>  DNA
<213>  Artificial sequence

<220>
<223>  Artificial DNA sequence containing SARS-CoV-2 target sequences

<400>  1
acatgttccc gggtagcggc cgcgagattt ggacctgcga gcgggttctg acctgaaggc       60

tctgcgcggg agcggctgtc tccacaagtg cggccgctac tgcagatgag cttagtcctg      120

ttgcactacg acagatgtct tgtgctgccg gtactacaca aactgcttgc actgatgaca      180

atgcgttagc ttactacaac acaacaaagg gagtaaccct gtgggtttta cacttaaaaa      240

cacagtctgt accgtctgcg gtatgtggaa aggttatggc tgtagttgtg atcaactccg      300

cgaacccatg cttcagtcag ctgatgcaca atcgttgagg taactggtat gatttcggtg      360

atttcataca aaccacgcca ggtagtggag ttcctgttgt agattcttat tattcattgt      420

taatgcctat attaaccttg accagatgag tgaaatggtc atgtgtggcg gttcactata      480

tgttaaacca ggtggaacct catcaggaga tgccacaact gcttatgcta atagtgtttt      540

taacatttgg agctgcaggg agatatcaga caggtacgtt aatagttaat agcgtacttc      600

tttttcttgc tttcgtggta ttcttgctag ttacactagc catccttact gcgcttcgat      660

tgtgtgcgta ctgctgcaat attgttgata tccaatgacc ccaaaatcag cgaaatcacc      720

ccgcattacg tttggtggac cctcagattc aactggcagt aaccagaatg gagaacgcag      780

tggggcgtaa ctgagggagc cttgaataca ccaaaagatc acattggcac ccgcaatcct      840

gctaacaatg ctgcaatcgt gctacaactt cctcaaggaa caacattgcc aaaaggcttc      900

tacgcagaag ggagcagagg cggcagtcaa gcctcttctc gttcctcatt agagtagggg      960

aacttctcct gctagaatgg ctggcaatgg cggtgatgct gctcttgctt tgctgctgct     1020

tgacagattg aaccagcttg agagcaaaat gtctggtaaa gccaaggaaa ttttggggac     1080

caggaactaa tcagacaagg aactgattac aaacattggc cgcaaattgc acaatttgcc     1140

cccagcgctt cagcgttctt cggaatgtcg cgcattggca tggaagtcac accttcggga     1200

acgtggttga cctacacagg tgccaggacc atgg                                 1234


<210>  2
<211>  720
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EGFP reporter gene

<400>  2
atggtgagca agggtgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac       60

ggcgacgtta acggccacaa gtttagcgtg tccggcgagg gcgagggcga tgccacctac      120

ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc      180

ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag      240

cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc      300

ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg      360

gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac      420

aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac      480

ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc      540

gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac      600

tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc      660

ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa      720


<210>  3
<211>  188
<212>  DNA
<213>  Tobacco Mosaic Virus

<400>  3
ttatagaaat aatataaaat taggtttgag agagaagatt acaaacgtga gagacggagg       60

gcccatggaa cttacagaag aagtcgttga tgagttcatg gaagatgtcc ctatgtcgat      120

caggcttgca aagtttcgat ctcgaaccgg aaaaaagagt gatgtccgca aagggaaaaa      180

tagtagta                                                               188


<210>  4
<211>  477
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA version of TMV coat protein (CP) gene

<400>  4
atgagttact ctattacaac tccgagtcaa tttgttttcc ttagttctgc ttgggctgat       60

ccgattgaac ttattaatct atgcacaaac gctctaggta atcaatttca aacacaacaa      120

gctcgcactg ttgtacaaag acaattctct gaggtttgga aaccgagtcc gcaagttaca      180

gtaagatttc cggattctga tttcaaggta taccgttaca acgctgttct tgatccgcta      240

gtaacagctc ttctaggagc tttcgatact agaaaccgta ttattgaagt agagaaccaa      300

gctaacccga caactgctga aacacttgat gctactcgtc gcgttgatga tgctacagta      360

gctattcgct ctgctattaa taaccttatt gttgaactaa ttcgtggtac tggaagttac      420

aaccgcagtt ctttcgagag ttctagtggt ctagtatgga caagtggacc ggctact         477


<210>  5
<211>  34
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TMV CP forward primer

<400>  5
atgagttact ctattacaac tccgagtcaa tttg                                   34


<210>  6
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  TMV CP reverse primer

<400>  6
agtagccggt ccacttgtcc a                                                 21


<210>  7
<211>  52
<212>  DNA
<213>  Artificial sequence

<220>
<223>  OriA forward primer

<400>  7
caagtaaaca tgtcccgggg aattatagaa ataatataaa attaggtttg ag               52


<210>  8
<211>  43
<212>  DNA
<213>  Artificial sequence

<220>
<223>  OriA reverse primer

<400>  8
ctctagagag ctcgactcga gtactactat ttttcccttt gcg                         43


<210>  9
<211>  33
<212>  DNA
<213>  Artificial sequence

<220>
<223>  EGFP forward primer

<400>  9
aaaaaaaaaa acatggtgag caagggtgag gag                                    33


<210>  10
<211>  288
<212>  DNA
<213>  Tomato Aspermy Virus

<400>  10
atggcaagca tcgagatccc tctacacgag atcattcgaa agttggaacg gatgaatcaa       60

aagaaacaag cacagaggaa acgacacaaa ctgaaccgca aggagcgggg tcacaaaagt      120

ccaagtgaac aaaggcgatc ggagttatgg cacgcgcgtc aagttgaact ttctgccatt      180

aattccgata attcttcaga tgagggtacc actctgtgtc gctttgacac atttggttcc      240

aagtctgatg ctatttgtga tcgctctgac tggtgtctcg atcaatga                   288


<210>  11
<211>  7392
<212>  DNA
<213>  Artificial sequence

<220>
<223>  pRIC4 vector

<400>  11
gaagagcgtg actccgaggg gttgcctcaa actctatctt ataaccggcg tggaggcatg       60

gaggcagggg tattttggtc attttaatag atagtggaaa atgacgtgga atttacttaa      120

agacgaagtc tttgcgacaa gggggggccc acgccgaatt taatattacc ggcgtggccc      180

ccccttatcg cgagtgcttt agcacgagcg gtccagattt aaagtagaaa atttcccgcc      240

cactagggtt aaaggtgttc acactataaa agcatatacg atgtgatggt atttgctcga      300

cctggtccaa agaccagagg gctattgaga cttttcaaca aagggtaata tcgggaaacc      360

tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag      420

atggcttcta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctcta      480

ccgacagtgg tcccaaagat ggacccccac ccacgaggaa catcgtggaa aaagaagacg      540

ttccaaccac gtcttcaaag caagtggatt gatgtgatac atggtggagc acgacactct      600

cgtctactcc aagaatatca aagatacagt ctcagaagac cagagggcta ttgagacttt      660

tcaacaaagg gtaatatcgg gaaacctcct cggattccat tgcccagcta tctgtcactt      720

catcgaaagg acagtagaaa aggaagatgg cttctacaaa tgccatcatt gcgataaagg      780

aaaggctatc gttcaagatg cctctaccga cagtggtccc aaagatggac ccccacccac      840

gaggaacatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg      900

tgatatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc      960

ctctatataa ggaagttcat ttcatttgga gaggacacgc tgagttcaca acacaaatca     1020

gatttataga gagatttata aaaaaaaaaa aacatgtgat cccgggaatt cgctagcact     1080

agtaagcttg tcgactcgag ctctctagag tccgcaaaaa tcaccagtct ctctctacaa     1140

atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt     1200

agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt     1260

gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac     1320

cgggcggcgg gtcgagaatg attattttat gaatatattt cattgtgcaa gtagatagaa     1380

attacatatg ttacataaca cacgaaataa acaaaaaaag acaatccaaa aacaaacacc     1440

ccaaaaaaaa taatcacttt agataaactc gtatgaggag aggcacgttc agtgactcga     1500

cgattcccga gcaaaaaaag tctccccgtc acacatatag tgggtgacgc aattatcttt     1560

aaagtaatcc ttctgttgac ttgtcattga taacatccag tcttcgtcag gattgcaaag     1620

aattatagaa gggatcccac cttttatttt cttctttttt ccatatttag ggttgacagt     1680

gaaatcagac tggcaaccta ttaattgctt ccacaatggg acgaacttga aggggatgtc     1740

gtcgatgata ttataggtgg cgtgttcatc gtagttggtg aaatcgatgg taccgttcca     1800

atagttgtgt cgtccgagac ttctagccca ggtggtcttt ccggtacgag ttggtccgca     1860

gatgtagagg ctggggtgtc ggattccatt ccttccattg tcctggttaa atcggccatc     1920

cattcaaggt cagattgagc ttgttggtat gagacaggat gtatgtaagt ataagcgtct     1980

atgcttacat ggtatagatg ggtttccctc caggagtgta gatcttcgtg gcagcgaaga     2040

tctgattctg tgaagggcga cacatacggt tcaggttgtg gagggaataa tttgttggct     2100

gaatattcca gccattgaag ttttgttgcc cattcatgag ggaattcttc cttgatcatg     2160

tcaagatatt cctccttaga cgttgcagtc tggataatag ttctccatcg tgcgtcagat     2220

ttgcgaggag agaccttatg atctcggaaa tctcctctgg ttttaatatc tccgtccttt     2280

gatatgtaat caaggacttg tttagagttt ctagctggct ggatattagg gtgatttcct     2340

tcaaaatcga aaaaagaagg atccctaata caaggttttt tatcaagctg gagaagagca     2400

tgatagtggg tagtgccatc ttgatgaagc tcagaagcaa caccaaggaa gaaaataaga     2460

aaaggtgtga gtttctccca gagaaactgg aataaatcat ctctttgaga tgagcacttg     2520

ggataggtaa ggaaaacata tttagattgg agtctgaagt tcttactagc agaaggcatg     2580

ttgttgtgac tccgaggggt tgcctcaaac tctatcttat aaccggcgtg gaggcatgga     2640

ggcaggggta ttttggtcat tttaatagat agtggaaaat gacgtggaat ttacttaaag     2700

acgaagtctt tgcgacaagg gggggcccac gccgaattta atattaccgg cgtggccccc     2760

ccttatcgcg agtgctttag cacgagcggt ccagatttaa agtagaaaat ttcccgccca     2820

ctagggttaa aggtgttcac actatacaag catatacgat gtgatggtat tgactagagt     2880

ttaaacgaat tcgagctcgg taccccccta ctccaaaaat gtcaaagata cagtctcaga     2940

agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc tcctcggatt     3000

ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag gtggctccta     3060

caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg ccgacagtgg     3120

tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac     3180

gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg acgcacaatc     3240

ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac     3300

agcccaagct tcgactctag aggatcccct taaatcgata tggcaagcat cgagatccct     3360

ctacacgaga tcattcgaaa gttggaacgg atgaatcaaa agaaacaagc acagaggaaa     3420

cgacacaaac tgaaccgcaa ggagcggggt cacaaaagtc caagtgaaca aaggcgatcg     3480

gagttatggc acgcgcgtca agttgaactt tctgccatta attccgataa ttcttcagat     3540

gagggtacca ctctgtgtcg ctttgacaca tttggttcca agtctgatgc tatttgtgat     3600

cgctctgact ggtgtctcga tcaatgacaa ttgctcgaat tcgctgaaat caccagtctc     3660

tctctacaaa tctatctctc tctattttct ccataaataa tgtgtgagta gtttcccgat     3720

aagggaaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta     3780

gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa     3840

atccagtact aaaatccaga tctcctaaag tccctataga tctttgtcgt gaatataaac     3900

cagacacgag acgactaaac ctggagccca gacgccgttc gaagctagaa gtaccgctta     3960

ggcaggaggc cgttagggaa aagatgctaa ggcagggttg gttacgttga ctcccccgta     4020

ggtttggttt aaatatgatg aagtggacgg aaggaaggag gaagacaagg aaggataagg     4080

ttgcaggccc tgtgcaaggt aagaagatgg aaatttgata gaggtacgct actatactta     4140

tactatacgc taagggaatg cttgtattta taccctatac cccctaataa ccccttatca     4200

atttaagaaa taatccgcat aagcccccgc ttaaaaattg gtatcagagc catgaatagg     4260

tctatgacca aaactcaaga ggataaaacc tcaccaaaat acgaaagagt tcttaactct     4320

aaagataaaa gatgtttaaa ctatcagtgt ttgacaggat atattggcgg gtaaacctaa     4380

gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa ggtttatccg     4440

ttcgtccatt tgtatgtgta catcaccgac gagcaaggca agaccgagcg cctttccgac     4500

gctcaccggg ctggttgccc tcgccgctgg gctggcggcc gtctatggcc ctgcaaacgc     4560

gccagaaacg ccgtcgaagc cgtgtgcgag acaccggccg ccggcgttgt ggatacctcg     4620

cggaaaactt ggccctcact gacagatgag gggcggacgt tgacacttga ggggccgact     4680

cacccggcgc ggcgttgaca gatgaggggc aggctcgatt tcggccggcg acgtggagct     4740

ggccagcctc gcaaatcggc gaaaacgcct gattttacgc gagtttccca cagatgatgt     4800

ggacaagcct ggggataagt gccctgcggt attgacactt gaggggcgcg actactgaca     4860

gatgaggggc gcgatccttg acacttgagg ggcagagtgc tgacagatga ggggcgcacc     4920

tattgacatt tgaggggctg tccacaggca gaaaatccag catttgcaag ggtttccgcc     4980

cgtttttcgg ccaccgctaa cctgtctttt aacctgcttt taaaccaata tttataaacc     5040

ttgtttttaa ccagggctgc gccctgtgcg cgtgaccgcg cacgccgaag gggggtgccc     5100

ccccttctcg aaccctcccg gcccgctaac gcgggcctcc catcccccca ggggctgcgc     5160

ccctcggccg cgaacggcct caccccaaaa atggcagcgc tggcagtcct tgccattgcc     5220

gggatcgggg cagtaacggg atgggcgatc agcccgaaag ctacccctat ttgtttattt     5280

ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa     5340

taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt     5400

tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat     5460

gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag     5520

atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg     5580

ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata     5640

cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat     5700

ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc     5760

aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg     5820

ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac     5880

gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact     5940

ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa     6000

gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct     6060

ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc     6120

tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga     6180

cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac     6240

tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag     6300

atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg     6360

tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc     6420

tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag     6480

ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt     6540

cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac     6600

ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc     6660

gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt     6720

tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt     6780

gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc     6840

ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt     6900

tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca     6960

ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt     7020

tgctggcctt ttgctcacat ggactctagc tagaggatca caggcagcaa cgctctgtca     7080

tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta     7140

gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct     7200

ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg     7260

agctgccggt cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa     7320

ttgacgctta gacaacttaa taacacattg cggacgtttt taatgatcga atactaacgt     7380

ctctaccaca tt                                                         7392


