                         SEQUENCE LISTING

<110>  Yissum Research Development Company of The Hebrew 
       University of Jerusalem Ltd.
 
<120>  SESAME PLANTS RESISTANT TO ACETOLACTATE SYNTHASE-INHIBITING 
       HERBICIDES, COMPOSITIONS AND METHODS FOR PRODUCING SAME

<130>  YISSUM/0174 PCT

<150>  63/208,077
<151>  2021-06-08

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  656
<212>  PRT
<213>  Sesamum indicum

<400>  1

Met Ala Ala Ala Thr Pro Ala Ser Ser Ser Ser Thr Val Trp Lys Arg 
1               5                   10                  15      


Ser Ser Phe Ser Asn Ser Ser Lys Leu Leu Ser Arg Phe Thr Val Pro 
            20                  25                  30          


Phe Pro His Asn Pro Gln Lys Pro Thr Ala Gly Val Ile Ser Gln Arg 
        35                  40                  45              


Arg Pro Leu Gln Ile Ser Asn Val Leu Ser Ala Pro Arg Glu Gln Val 
    50                  55                  60                  


His Pro Glu Glu Ala Phe Val Ser Arg Phe Ala Ala Asp Glu Pro Arg 
65                  70                  75                  80  


Lys Gly Cys Asp Val Leu Val Glu Ala Leu Glu Arg Glu Gly Val Thr 
                85                  90                  95      


Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met Glu Ile His Gln Ala 
            100                 105                 110         


Leu Thr Arg Ser Lys Ile Ile Arg Asn Val Leu Pro Arg His Glu Gln 
        115                 120                 125             


Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg Ala Ser Gly Leu Pro 
    130                 135                 140                 


Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val Ser 
145                 150                 155                 160 


Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro Leu Val Ala Ile Thr 
                165                 170                 175     


Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp Ala Phe Gln Glu Thr 
            180                 185                 190         


Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys His Asn Tyr Leu Val 
        195                 200                 205             


Leu Asp Val Glu Asp Ile Pro Arg Val Val Lys Glu Ala Phe Phe Ile 
    210                 215                 220                 


Ala Arg Ser Gly Arg Pro Gly Pro Val Leu Ile Asp Val Pro Lys Asp 
225                 230                 235                 240 


Ile Gln Gln Gln Met Val Val Pro Asn Trp Asn Gln Pro Met Arg Leu 
                245                 250                 255     


Ala Gly Tyr Leu Ser Arg Leu Pro Arg Pro Pro Ser Ala Met Leu Leu 
            260                 265                 270         


Glu Gln Ile Val Arg Leu Ile Ser Glu Ser Lys Lys Pro Val Leu Tyr 
        275                 280                 285             


Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val 
    290                 295                 300                 


Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser 
305                 310                 315                 320 


Tyr Pro Cys Ser Asp Glu Glu Phe Ala Leu Gln Met Leu Gly Met His 
                325                 330                 335     


Gly Thr Val Tyr Ala Asn Tyr Ala Val Asp Lys Ser Asp Leu Leu Leu 
            340                 345                 350         


Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala 
        355                 360                 365             


Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu 
    370                 375                 380                 


Ile Gly Lys Asn Lys Gln Pro His Val Ser Ile Cys Ala Asp Met Lys 
385                 390                 395                 400 


Leu Ala Leu Gln Gly Leu Asn Leu Ile Leu Glu Asp Met Leu Lys Glu 
                405                 410                 415     


Gly Lys Asn Asn Val Asp Phe Ser Ala Trp Arg Gln Glu Leu Lys Glu 
            420                 425                 430         


Gln Lys Met Asn Phe Pro Leu Ser Phe Lys Thr Phe Glu Asp Ala Ile 
        435                 440                 445             


Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Gly Gly Asn 
    450                 455                 460                 


Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala Ala Gln 
465                 470                 475                 480 


Phe Tyr Lys Tyr Asn Lys Pro Arg Gln Trp Leu Thr Ser Gly Gly Leu 
                485                 490                 495     


Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala Val Ala 
            500                 505                 510         


Arg Pro Asp Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser Phe Ile 
        515                 520                 525             


Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu Pro Val 
    530                 535                 540                 


Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val Gln Trp 
545                 550                 555                 560 


Glu Asp Arg Phe Tyr Lys Ser Asn Arg Ala His Thr Tyr Leu Gly Asn 
                565                 570                 575     


Pro Ser Lys Glu Ser Glu Ile Phe Pro Asp Met Leu Lys Phe Ala Glu 
            580                 585                 590         


Ala Cys Asp Ile Pro Ala Ala Arg Val Thr Lys Lys Asp Asp Leu Arg 
        595                 600                 605             


Ala Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu Leu Asp 
    610                 615                 620                 


Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro Ser Gly 
625                 630                 635                 640 


Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Ile Lys Tyr 
                645                 650                 655     


<210>  2
<211>  2394
<212>  DNA
<213>  Sesamum indicum

<400>  2
aagggcaatt ccgacatttc cctctgctgc ctcctgaata agttccgcct tccaaatcaa       60

acgtgcttcc cttttcctcg ccacagaaaa ccctaaggag ctacagaccc gccgtcgccg      120

ccgatttgca ttctctaacg tccgtcggaa atggcggccg ctactcctgc ctccagctcg      180

tccactgtgt ggaaacgctc ttctttctct aattcgtcga agcttctttc tagatttacc      240

gtcccgttcc ctcacaatcc tcagaagccc acagctggcg tcatttcgca acgccgtccg      300

ctgcaaatct caaatgtgct gtctgctccc agggaacaag tgcatcctga ggaggcgttt      360

gtttcccgat ttgccgctga cgaacctcgg aagggctgcg acgttttagt ggaggcgctt      420

gaacgagaag gggtcaccga cgtctttgct taccccggag gcgcgtctat ggagatccat      480

caagcgctta cccgctccaa aatcatccgg aacgtgctcc cgcgccacga gcagggaggc      540

gtcttcgctg ccgaggggta cgcccgcgct tccggtcttc ccggcgtctg catcgcgaca      600

tctggccctg gtgccaccaa tttggtctct ggcctcgccg atgctcttct cgatagcgta      660

ccgcttgtgg ctatcacagg ccaggttccc cgtcgtatga tcggcactga tgctttccaa      720

gaaaccccta ttgttgaggt aactaggtcg attaccaagc ataattatct tgttttagat      780

gttgaggata ttcctcgggt tgtcaaggaa gctttcttta tcgctcggtc gggaaggcct      840

ggcccagttt tgattgacgt tccaaaagat attcaacagc aaatggtggt accaaattgg      900

aatcagccaa tgaggttggc tggttacttg tctagattgc ctaggccgcc tagtgcgatg      960

ttgttggagc agatagttag gctgatatcc gagtccaaaa agccggtact ttatgtcggt     1020

ggtgggtgtt tgaattcaag tgaggagttg aggaggtttg tggagctcac aggaattccg     1080

gttgctagca cgcttatggg tcttggttct tatccttgtt ctgatgagga atttgcgctg     1140

caaatgctgg gtatgcatgg gactgtttat gcaaattatg cagtggacaa gagtgatttg     1200

ttgcttgctt ttggagtcag gtttgatgac cgtgtgacgg gtaagctgga ggcatttgcc     1260

agtcgagcaa agattgtcca cattgacatt gattctgctg agattggaaa gaataagcaa     1320

cctcatgtgt ccatttgtgc cgatatgaag ttagctttgc agggtttgaa cttgatattg     1380

gaagacatgc tgaaagaggg taagaacaat gttgactttt cagcctggag gcaagagtta     1440

aaagagcaga agatgaactt tcctttgagt ttcaagacat ttgaagatgc cattccacct     1500

cagtatgcaa ttcaagttct tgatgaactg acaggaggga atgcaatcat tagtactggt     1560

gttgggcagc atcagatgtg ggctgcacaa ttttataagt ataataagcc acgacagtgg     1620

ttgacatcag gtggtttagg ggcaatgggt ttcggacttc ctgcagcaat tggagctgct     1680

gttgcaagac cagatgcagt tgtagtggat attgatggtg atggaagttt tataatgaac     1740

gtgcaggagt tggccactat ccgggtagag aaccttcctg ttaagattat gttgttaaat     1800

aatcagcact tgggtatggt tgtccagtgg gaagaccgct tctataagtc caacagggca     1860

cacacttatc tcggaaatcc ttccaaagag tcagagattt ttccagatat gttgaagttt     1920

gcagaggctt gtgatatacc tgcagctcgt gtgacaaaga aggatgatct cagggcagca     1980

atacaaaaga tgttggatac tcctgggcca tatttgttgg atgtcattgt accccatcaa     2040

gagcatgtct tgcccatgat tcccagtgga ggagccttta aagacgtgat cacagagggt     2100

gatggaagaa ttaagtattg atgtgaagta cggcagagcg gccatttggt tgtgaaaatt     2160

aggtcgcagc taaagggggt ccaagtttat agttttggct tgtaagttgt gttgtgttct     2220

gacatttgca aggtctgatt gtagtacgtc tgctctttat gcttattggt attcagtgat     2280

acctatgttc atttatcaca ttctgtttaa tctattataa taacggttgg atgctcggct     2340

ctattcatgt attttgataa ttatgtagag aaacgcatcc acctaatttg ttaa           2394


<210>  3
<211>  656
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Mutant acetolactate synthase protein

<400>  3

Met Ala Ala Ala Thr Pro Ala Ser Ser Ser Ser Thr Val Trp Lys Arg 
1               5                   10                  15      


Ser Ser Phe Ser Asn Ser Ser Lys Leu Leu Ser Arg Phe Thr Val Pro 
            20                  25                  30          


Phe Pro His Asn Pro Gln Lys Pro Thr Ala Gly Val Ile Ser Gln Arg 
        35                  40                  45              


Arg Pro Leu Gln Ile Ser Asn Val Leu Ser Ala Pro Arg Glu Gln Val 
    50                  55                  60                  


His Pro Glu Glu Ala Phe Val Ser Arg Phe Ala Ala Asp Glu Pro Arg 
65                  70                  75                  80  


Lys Gly Cys Asp Val Leu Val Glu Ala Leu Glu Arg Glu Gly Val Thr 
                85                  90                  95      


Asp Val Phe Ala Tyr Pro Gly Gly Ala Ser Met Glu Ile His Gln Ala 
            100                 105                 110         


Leu Thr Arg Ser Lys Ile Ile Arg Asn Val Leu Pro Arg His Glu Gln 
        115                 120                 125             


Gly Gly Val Phe Ala Ala Glu Gly Tyr Ala Arg Ala Ser Gly Leu Pro 
    130                 135                 140                 


Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val Ser 
145                 150                 155                 160 


Gly Leu Ala Asp Ala Leu Leu Asp Ser Val Pro Leu Val Ala Ile Thr 
                165                 170                 175     


Gly Gln Val Pro Arg Arg Met Ile Gly Thr Asp Val Phe Gln Glu Thr 
            180                 185                 190         


Pro Ile Val Glu Val Thr Arg Ser Ile Thr Lys His Asn Tyr Leu Val 
        195                 200                 205             


Leu Asp Val Glu Asp Ile Pro Arg Val Val Lys Glu Ala Phe Phe Ile 
    210                 215                 220                 


Ala Arg Ser Gly Arg Pro Gly Pro Val Leu Ile Asp Val Pro Lys Asp 
225                 230                 235                 240 


Ile Gln Gln Gln Met Val Val Pro Asn Trp Asn Gln Pro Met Arg Leu 
                245                 250                 255     


Ala Gly Tyr Leu Ser Arg Leu Pro Arg Pro Pro Ser Ala Met Leu Leu 
            260                 265                 270         


Glu Gln Ile Val Arg Leu Ile Ser Glu Ser Lys Lys Pro Val Leu Tyr 
        275                 280                 285             


Val Gly Gly Gly Cys Leu Asn Ser Ser Glu Glu Leu Arg Arg Phe Val 
    290                 295                 300                 


Glu Leu Thr Gly Ile Pro Val Ala Ser Thr Leu Met Gly Leu Gly Ser 
305                 310                 315                 320 


Tyr Pro Cys Ser Asp Glu Glu Phe Ala Leu Gln Met Leu Gly Met His 
                325                 330                 335     


Gly Thr Val Tyr Ala Asn Tyr Ala Val Asp Lys Ser Asp Leu Leu Leu 
            340                 345                 350         


Ala Phe Gly Val Arg Phe Asp Asp Arg Val Thr Gly Lys Leu Glu Ala 
        355                 360                 365             


Phe Ala Ser Arg Ala Lys Ile Val His Ile Asp Ile Asp Ser Ala Glu 
    370                 375                 380                 


Ile Gly Lys Asn Lys Gln Pro His Val Ser Ile Cys Ala Asp Met Lys 
385                 390                 395                 400 


Leu Ala Leu Gln Gly Leu Asn Leu Ile Leu Glu Asp Met Leu Lys Glu 
                405                 410                 415     


Gly Lys Asn Asn Val Asp Phe Ser Ala Trp Arg Gln Glu Leu Lys Glu 
            420                 425                 430         


Gln Lys Met Asn Phe Pro Leu Ser Phe Lys Thr Phe Glu Asp Ala Ile 
        435                 440                 445             


Pro Pro Gln Tyr Ala Ile Gln Val Leu Asp Glu Leu Thr Gly Gly Asn 
    450                 455                 460                 


Ala Ile Ile Ser Thr Gly Val Gly Gln His Gln Met Trp Ala Ala Gln 
465                 470                 475                 480 


Phe Tyr Lys Tyr Asn Lys Pro Arg Gln Trp Leu Thr Ser Gly Gly Leu 
                485                 490                 495     


Gly Ala Met Gly Phe Gly Leu Pro Ala Ala Ile Gly Ala Ala Val Ala 
            500                 505                 510         


Arg Pro Asp Ala Val Val Val Asp Ile Asp Gly Asp Gly Ser Phe Ile 
        515                 520                 525             


Met Asn Val Gln Glu Leu Ala Thr Ile Arg Val Glu Asn Leu Pro Val 
    530                 535                 540                 


Lys Ile Met Leu Leu Asn Asn Gln His Leu Gly Met Val Val Gln Trp 
545                 550                 555                 560 


Glu Asp Arg Phe Tyr Lys Ser Asn Arg Ala His Thr Tyr Leu Gly Asn 
                565                 570                 575     


Pro Ser Lys Glu Ser Glu Ile Phe Pro Asp Met Leu Lys Phe Ala Glu 
            580                 585                 590         


Ala Cys Asp Ile Pro Ala Ala Arg Val Thr Lys Lys Asp Asp Leu Arg 
        595                 600                 605             


Ala Ala Ile Gln Lys Met Leu Asp Thr Pro Gly Pro Tyr Leu Leu Asp 
    610                 615                 620                 


Val Ile Val Pro His Gln Glu His Val Leu Pro Met Ile Pro Ser Gly 
625                 630                 635                 640 


Gly Ala Phe Lys Asp Val Ile Thr Glu Gly Asp Gly Arg Ile Lys Tyr 
                645                 650                 655     


<210>  4
<211>  2394
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide encoding mutant ALS protein

<400>  4
aagggcaatt ccgacatttc cctctgctgc ctcctgaata agttccgcct tccaaatcaa       60

acgtgcttcc cttttcctcg ccacagaaaa ccctaaggag ctacagaccc gccgtcgccg      120

ccgatttgca ttctctaacg tccgtcggaa atggcggccg ctactcctgc ctccagctcg      180

tccactgtgt ggaaacgctc ttctttctct aattcgtcga agcttctttc tagatttacc      240

gtcccgttcc ctcacaatcc tcagaagccc acagctggcg tcatttcgca acgccgtccg      300

ctgcaaatct caaatgtgct gtctgctccc agggaacaag tgcatcctga ggaggcgttt      360

gtttcccgat ttgccgctga cgaacctcgg aagggctgcg acgttttagt ggaggcgctt      420

gaacgagaag gggtcaccga cgtctttgct taccccggag gcgcgtctat ggagatccat      480

caagcgctta cccgctccaa aatcatccgg aacgtgctcc cgcgccacga gcagggaggc      540

gtcttcgctg ccgaggggta cgcccgcgct tccggtcttc ccggcgtctg catcgcgaca      600

tctggccctg gtgccaccaa tttggtctct ggcctcgccg atgctcttct cgatagcgta      660

ccgcttgtgg ctatcacagg ccaggttccc cgtcgtatga tcggcactga tgttttccaa      720

gaaaccccta ttgttgaggt aactaggtcg attaccaagc ataattatct tgttttagat      780

gttgaggata ttcctcgggt tgtcaaggaa gctttcttta tcgctcggtc gggaaggcct      840

ggcccagttt tgattgacgt tccaaaagat attcaacagc aaatggtggt accaaattgg      900

aatcagccaa tgaggttggc tggttacttg tctagattgc ctaggccgcc tagtgcgatg      960

ttgttggagc agatagttag gctgatatcc gagtccaaaa agccggtact ttatgtcggt     1020

ggtgggtgtt tgaattcaag tgaggagttg aggaggtttg tggagctcac aggaattccg     1080

gttgctagca cgcttatggg tcttggttct tatccttgtt ctgatgagga atttgcgctg     1140

caaatgctgg gtatgcatgg gactgtttat gcaaattatg cagtggacaa gagtgatttg     1200

ttgcttgctt ttggagtcag gtttgatgac cgtgtgacgg gtaagctgga ggcatttgcc     1260

agtcgagcaa agattgtcca cattgacatt gattctgctg agattggaaa gaataagcaa     1320

cctcatgtgt ccatttgtgc cgatatgaag ttagctttgc agggtttgaa cttgatattg     1380

gaagacatgc tgaaagaggg taagaacaat gttgactttt cagcctggag gcaagagtta     1440

aaagagcaga agatgaactt tcctttgagt ttcaagacat ttgaagatgc cattccacct     1500

cagtatgcaa ttcaagttct tgatgaactg acaggaggga atgcaatcat tagtactggt     1560

gttgggcagc atcagatgtg ggctgcacaa ttttataagt ataataagcc acgacagtgg     1620

ttgacatcag gtggtttagg ggcaatgggt ttcggacttc ctgcagcaat tggagctgct     1680

gttgcaagac cagatgcagt tgtagtggat attgatggtg atggaagttt tataatgaac     1740

gtgcaggagt tggccactat ccgggtagag aaccttcctg ttaagattat gttgttaaat     1800

aatcagcact tgggtatggt tgtccagtgg gaagaccgct tctataagtc caacagggca     1860

cacacttatc tcggaaatcc ttccaaagag tcagagattt ttccagatat gttgaagttt     1920

gcagaggctt gtgatatacc tgcagctcgt gtgacaaaga aggatgatct cagggcagca     1980

atacaaaaga tgttggatac tcctgggcca tatttgttgg atgtcattgt accccatcaa     2040

gagcatgtct tgcccatgat tcccagtgga ggagccttta aagacgtgat cacagagggt     2100

gatggaagaa ttaagtattg atgtgaagta cggcagagcg gccatttggt tgtgaaaatt     2160

aggtcgcagc taaagggggt ccaagtttat agttttggct tgtaagttgt gttgtgttct     2220

gacatttgca aggtctgatt gtagtacgtc tgctctttat gcttattggt attcagtgat     2280

acctatgttc atttatcaca ttctgtttaa tctattataa taacggttgg atgctcggct     2340

ctattcatgt attttgataa ttatgtagag aaacgcatcc acctaatttg ttaa           2394


<210>  5
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
caggttcccc gtcgtatg                                                     18


<210>  6
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
tccttgacaa cccgagga                                                     18


<210>  7
<211>  92
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid marker

<400>  7
atcggcactg atgttttcca agaaacccct attgttgagg taactaggtc gattaccaag       60

cataattatc ttgttttaga tgttgaggat at                                     92


<210>  8
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
cccactcagc tgcctcat                                                     18


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
gcgaggccag agaccaaa                                                     18


<210>  10
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
agggaggtgt cttcgctg                                                     18


<210>  11
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
cctccagctt acccgtca                                                     18


<210>  12
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
gctgggtatg catgggac                                                     18


<210>  13
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
ttgtcacacg agctgcag                                                     18


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
cagcacttgg gtatggttgt                                                   20


<210>  15
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
ttcgagtggg atgtgcc                                                      17


