               SEQUENCE LISTING

<110> Abiopep, S.L.
      CONSEJO SUPERIOR DE INVESTIGACIONES CIENTÍFICAS (CSIC)

<120> Plants resistant to infection by pepino mosaic virus

<130> PCT4185.1

<150> ES P202130569
<151> 2021-06-18

<160> 9

<170> BiSSAP 1.3.6

<210> 1
<211> 831
<212> PRT
<213> Solanum lycopersicum


<400> 1
Met Ile Gln Ser Ser Phe Ser Ala Asp Ser Pro Ser Met Ala Ala Asn 
1               5                   10                  15      
Ser Thr Phe Ser Pro Pro Pro Ala Ala Gly Asp Gly Asp Phe Asn Tyr 
            20                  25                  30          
Asp Val Ala Trp Tyr Gly Asn Ile Gln Tyr Leu Leu Asn Ile Ser Ala 
        35                  40                  45              
Ile Gly Ala Leu Thr Cys Leu Leu Ile Phe Ile Phe Gly Lys Leu Arg 
    50                  55                  60                  
Ser Asp His Arg Arg Met Pro Gly Pro Thr Ala Ile Val Ser Lys Leu 
65                  70                  75                  80  
Leu Ala Ala Trp His Ala Thr Gly Val Glu Ile Ala Arg His Cys Gly 
                85                  90                  95      
Ala Asp Ala Ala Gln Tyr Leu Leu Ile Glu Gly Gly Ser Ser Ala Leu 
            100                 105                 110         
Leu Leu Phe Leu Ala Leu Leu Ser Leu Ala Val Met Leu Pro Leu Asn 
        115                 120                 125             
Ile Tyr Ala Gly Lys Ala Pro Met Ala Asp Gln Phe Ser Lys Thr Thr 
    130                 135                 140                 
Ile Asn His Ile Glu Lys Gly Ser Pro Leu Leu Trp Ile His Phe Ile 
145                 150                 155                 160 
Phe Val Val Ile Val Val Val Leu Val His Tyr Gly Ile Ser Glu Ile 
                165                 170                 175     
Gln Glu Arg Leu Lys Ile Thr Arg Leu Arg Asp Gly Tyr Gly Asn Pro 
            180                 185                 190         
Ser Asn Ser Gly Thr Asn Val Ser Ala Ile Phe Ser Ile Met Val Gln 
        195                 200                 205             
Gly Val Pro Lys Thr Leu Gly Phe Asp Lys Thr Pro Leu Val Glu Tyr 
    210                 215                 220                 
Phe Gln His Lys Tyr Pro Gly Lys Val Tyr Arg Val Val Val Pro Met 
225                 230                 235                 240 
Asp Leu Cys Ala Leu Asp Asp Leu Ala Thr Glu Leu Val Lys Val Arg 
                245                 250                 255     
Glu Asp Ile Ser Lys Leu Val Ser Arg Ile Glu Leu Arg Gly Tyr Leu 
            260                 265                 270         
Asn Glu Gly Glu Glu Asp Glu Tyr Asn Asn Asp Ser Val Asn Gly Arg 
        275                 280                 285             
Gly Leu Leu Glu Arg Leu Cys Phe Leu Trp Arg Lys Ala Lys Asp Thr 
    290                 295                 300                 
Trp Tyr His Val Val Asp Gln Leu Gly Phe Ser Asp Glu Glu Arg Leu 
305                 310                 315                 320 
Arg Lys Leu Gln Glu Leu Arg Ala Asp Leu Glu Met Glu Met Ala Ser 
                325                 330                 335     
Tyr Lys Glu Gly Arg Ala Arg Gly Ala Gly Val Ala Phe Val Val Phe 
            340                 345                 350         
Lys Asp Val Phe Thr Ala Asn Lys Ala Val Gln Asp Leu Arg Asn Glu 
        355                 360                 365             
Lys Arg Arg Arg Tyr Gly Arg Phe Phe Ser Val Ile Glu Leu Gln Leu 
    370                 375                 380                 
Gln Arg Asn Gln Trp Lys Val Glu Arg Ala Pro Leu Ala Thr Asp Ile 
385                 390                 395                 400 
Tyr Trp Asn His Leu Gly Ser Thr Lys Phe Ser Leu Lys Leu Arg Arg 
                405                 410                 415     
Val Leu Val Asn Thr Cys Leu Leu Leu Met Leu Leu Phe Cys Ser Ser 
            420                 425                 430         
Pro Leu Ala Val Ile Ser Ala Ile Gln Ser Ala Gly Arg Ile Ile Asn 
        435                 440                 445             
Ala Glu Ala Met Asp His Ala Gln Met Trp Leu Asn Trp Val Gln Gly 
    450                 455                 460                 
Ser Ser Trp Leu Ala Thr Ile Ile Phe Gln Phe Leu Pro Asn Val Leu 
465                 470                 475                 480 
Ile Phe Val Ser Met Tyr Ile Val Val Pro Ser Val Leu Ser Tyr Leu 
                485                 490                 495     
Ser Lys Phe Glu Gln His Leu Thr Val Ser Gly Glu Gln Arg Ala Glu 
            500                 505                 510         
Leu Leu Lys Met Val Cys Phe Phe Leu Val Asn Leu Ile Leu Leu Arg 
        515                 520                 525             
Ala Leu Val Glu Ser Thr Leu Glu Gly Ala Leu Leu Ser Met Gly Arg 
    530                 535                 540                 
Cys Tyr Leu Asp Gly Glu Asp Cys Lys Lys Ile Glu Gln Tyr Met Thr 
545                 550                 555                 560 
Ala Ser Phe Leu Thr Arg Thr Cys Leu Ser Ser Leu Ala Phe Leu Ile 
                565                 570                 575     
Thr Ser Ser Phe Leu Gly Ile Ser Phe Asp Leu Leu Ala Pro Ile Pro 
            580                 585                 590         
Trp Ile Lys Lys Lys Leu Gln Lys Phe Arg Lys Asn Asp Met Leu Gln 
        595                 600                 605             
Leu Val Pro Glu Arg Ser Glu Glu Tyr Pro Leu Glu Asn Gln Asp Ile 
    610                 615                 620                 
Asp Ser Leu Glu Arg Pro Leu Ile His Glu Arg Ser Ser Thr Val Ile 
625                 630                 635                 640 
Ala Asp Asn Asn Gly Phe Leu His Asp Ala Ser Pro Asn Glu Ile Asp 
                645                 650                 655     
Phe Pro Gly Gln Asp Leu Ser Glu Tyr Pro Pro Val Ser Arg Thr Ser 
            660                 665                 670         
Pro Val Pro Lys Pro Lys Phe Asp Phe Ala Gln Tyr Tyr Ala Phe Asn 
        675                 680                 685             
Leu Thr Ile Phe Ala Leu Thr Leu Ile Tyr Cys Ser Phe Ala Pro Leu 
    690                 695                 700                 
Val Val Pro Val Gly Ala Val Tyr Phe Gly Tyr Arg Tyr Leu Val Asp 
705                 710                 715                 720 
Lys Tyr Asn Phe Leu Phe Val Tyr Arg Val Arg Gly Phe Pro Ala Gly 
                725                 730                 735     
Asn Asp Gly Arg Leu Met Asp Thr Val Leu Ser Ile Met Arg Phe Cys 
            740                 745                 750         
Val Asp Leu Phe Leu Leu Ser Met Leu Leu Phe Phe Ser Val Arg Gly 
        755                 760                 765             
Asp Ser Thr Lys Leu Gln Ala Ile Phe Thr Leu Gly Leu Leu Val Val 
    770                 775                 780                 
Tyr Lys Leu Leu Pro Ser Asp Lys Asp Ser Phe Gln Pro Ala Leu Leu 
785                 790                 795                 800 
Gln Gly Ile Gln Thr Ile Asp Asn Ile Val Glu Gly Pro Thr Asp Tyr 
                805                 810                 815     
Glu Val Phe Ser Gln Pro Thr Phe Asp Trp Asp Thr Tyr Asn Ser 
            820                 825                 830     

<210> 2
<211> 553
<212> PRT
<213> Artificial Sequence


<220> 
<223> EMS mutant

<400> 2
Met Ile Gln Ser Ser Phe Ser Ala Asp Ser Pro Ser Met Ala Ala Asn 
1               5                   10                  15      
Ser Thr Phe Ser Pro Pro Pro Ala Ala Gly Asp Gly Asp Phe Asn Tyr 
            20                  25                  30          
Asp Val Ala Trp Tyr Gly Asn Ile Gln Tyr Leu Leu Asn Ile Ser Ala 
        35                  40                  45              
Ile Gly Ala Leu Thr Cys Leu Leu Ile Phe Ile Phe Gly Lys Leu Arg 
    50                  55                  60                  
Ser Asp His Arg Arg Met Pro Gly Pro Thr Ala Ile Val Ser Lys Leu 
65                  70                  75                  80  
Leu Ala Ala Trp His Ala Thr Gly Val Glu Ile Ala Arg His Cys Gly 
                85                  90                  95      
Ala Asp Ala Ala Gln Tyr Leu Leu Ile Glu Gly Gly Ser Ser Ala Leu 
            100                 105                 110         
Leu Leu Phe Leu Ala Leu Leu Ser Leu Ala Val Met Leu Pro Leu Asn 
        115                 120                 125             
Ile Tyr Ala Gly Lys Ala Pro Met Ala Asp Gln Phe Ser Lys Thr Thr 
    130                 135                 140                 
Ile Asn His Ile Glu Lys Gly Ser Pro Leu Leu Trp Ile His Phe Ile 
145                 150                 155                 160 
Phe Val Val Ile Val Val Val Leu Val His Tyr Gly Ile Ser Glu Ile 
                165                 170                 175     
Gln Glu Arg Leu Lys Ile Thr Arg Leu Arg Asp Gly Tyr Gly Asn Pro 
            180                 185                 190         
Ser Asn Ser Gly Thr Asn Val Ser Ala Ile Phe Ser Ile Met Val Gln 
        195                 200                 205             
Gly Val Pro Lys Thr Leu Gly Phe Asp Lys Thr Pro Leu Val Glu Tyr 
    210                 215                 220                 
Phe Gln His Lys Tyr Pro Gly Lys Val Tyr Arg Val Val Val Pro Met 
225                 230                 235                 240 
Asp Leu Cys Ala Leu Asp Asp Leu Ala Thr Glu Leu Val Lys Val Arg 
                245                 250                 255     
Glu Asp Ile Ser Lys Leu Val Ser Arg Ile Glu Leu Arg Gly Tyr Leu 
            260                 265                 270         
Asn Glu Gly Glu Glu Asp Glu Tyr Asn Asn Asp Ser Val Asn Gly Arg 
        275                 280                 285             
Gly Leu Leu Glu Arg Leu Cys Phe Leu Trp Arg Lys Ala Lys Asp Thr 
    290                 295                 300                 
Trp Tyr His Val Val Asp Gln Leu Gly Phe Ser Asp Glu Glu Arg Leu 
305                 310                 315                 320 
Arg Lys Leu Gln Glu Leu Arg Ala Asp Leu Glu Met Glu Met Ala Ser 
                325                 330                 335     
Tyr Lys Glu Gly Arg Ala Arg Gly Ala Gly Val Ala Phe Val Val Phe 
            340                 345                 350         
Lys Asp Val Phe Thr Ala Asn Lys Ala Val Gln Asp Leu Arg Asn Glu 
        355                 360                 365             
Lys Arg Arg Arg Tyr Gly Arg Phe Phe Ser Val Ile Glu Leu Gln Leu 
    370                 375                 380                 
Gln Arg Asn Gln Trp Lys Val Glu Arg Ala Pro Leu Ala Thr Asp Ile 
385                 390                 395                 400 
Tyr Trp Asn His Leu Gly Ser Thr Lys Phe Ser Leu Lys Leu Arg Arg 
                405                 410                 415     
Val Leu Val Asn Thr Cys Leu Leu Leu Met Leu Leu Phe Cys Ser Ser 
            420                 425                 430         
Pro Leu Ala Val Ile Ser Ala Ile Gln Ser Ala Gly Arg Ile Ile Asn 
        435                 440                 445             
Ala Glu Ala Met Asp His Ala Gln Met Trp Leu Asn Trp Val Gln Gly 
    450                 455                 460                 
Ser Ser Trp Leu Ala Thr Ile Ile Phe Gln Phe Leu Pro Asn Val Leu 
465                 470                 475                 480 
Ile Phe Val Ser Met Tyr Ile Val Val Pro Ser Val Leu Ser Tyr Leu 
                485                 490                 495     
Ser Lys Phe Glu Gln His Leu Thr Val Ser Gly Glu Gln Arg Ala Glu 
            500                 505                 510         
Leu Leu Lys Met Val Cys Phe Phe Leu Val Asn Leu Ile Leu Leu Arg 
        515                 520                 525             
Ala Leu Val Glu Ser Thr Leu Glu Gly Ala Leu Leu Ser Met Gly Arg 
    530                 535                 540                 
Cys Tyr Leu Asp Gly Glu Asp Cys Lys 
545                 550             

<210> 3
<211> 434
<212> PRT
<213> Artificial Sequence


<220> 
<223> CRISPR/Cas9 mutant

<400> 3
Met Ile Gln Ser Ser Phe Ser Ala Asp Ser Gln Lys Phe Ser Leu Lys 
1               5                   10                  15      
Leu Arg Arg Val Leu Val Asn Thr Cys Leu Leu Leu Met Leu Leu Phe 
            20                  25                  30          
Cys Ser Ser Pro Leu Ala Val Ile Ser Ala Ile Gln Ser Ala Gly Arg 
        35                  40                  45              
Ile Ile Asn Ala Glu Ala Met Asp His Ala Gln Met Trp Leu Asn Trp 
    50                  55                  60                  
Val Gln Gly Ser Ser Trp Leu Ala Thr Ile Ile Phe Gln Phe Leu Pro 
65                  70                  75                  80  
Asn Val Leu Ile Phe Val Ser Met Tyr Ile Val Val Pro Ser Val Leu 
                85                  90                  95      
Ser Tyr Leu Ser Lys Phe Glu Gln His Leu Thr Val Ser Gly Glu Gln 
            100                 105                 110         
Arg Ala Glu Leu Leu Lys Met Val Cys Phe Phe Leu Val Asn Leu Ile 
        115                 120                 125             
Leu Leu Arg Ala Leu Val Glu Ser Thr Leu Glu Gly Ala Leu Leu Ser 
    130                 135                 140                 
Met Gly Arg Cys Tyr Leu Asp Gly Glu Asp Cys Lys Lys Ile Glu Gln 
145                 150                 155                 160 
Tyr Met Thr Ala Ser Phe Leu Thr Arg Thr Cys Leu Ser Ser Leu Ala 
                165                 170                 175     
Phe Leu Ile Thr Ser Ser Phe Leu Gly Ile Ser Phe Asp Leu Leu Ala 
            180                 185                 190         
Pro Ile Pro Trp Ile Lys Lys Lys Leu Gln Lys Phe Arg Lys Asn Asp 
        195                 200                 205             
Met Leu Gln Leu Val Pro Glu Arg Ser Glu Glu Tyr Pro Leu Glu Asn 
    210                 215                 220                 
Gln Asp Ile Asp Ser Leu Glu Arg Pro Leu Ile His Glu Arg Ser Ser 
225                 230                 235                 240 
Thr Val Ile Ala Asp Asn Asn Gly Phe Leu His Asp Ala Ser Pro Asn 
                245                 250                 255     
Glu Ile Asp Phe Pro Gly Gln Asp Leu Ser Glu Tyr Pro Pro Val Ser 
            260                 265                 270         
Arg Thr Ser Pro Val Pro Lys Pro Lys Phe Asp Phe Ala Gln Tyr Tyr 
        275                 280                 285             
Ala Phe Asn Leu Thr Ile Phe Ala Leu Thr Leu Ile Tyr Cys Ser Phe 
    290                 295                 300                 
Ala Pro Leu Val Val Pro Val Gly Ala Val Tyr Phe Gly Tyr Arg Tyr 
305                 310                 315                 320 
Leu Val Asp Lys Tyr Asn Phe Leu Phe Val Tyr Arg Val Arg Gly Phe 
                325                 330                 335     
Pro Ala Gly Asn Asp Gly Arg Leu Met Asp Thr Val Leu Ser Ile Met 
            340                 345                 350         
Arg Phe Cys Val Asp Leu Phe Leu Leu Ser Met Leu Leu Phe Phe Ser 
        355                 360                 365             
Val Arg Gly Asp Ser Thr Lys Leu Gln Ala Ile Phe Thr Leu Gly Leu 
    370                 375                 380                 
Leu Val Val Tyr Lys Leu Leu Pro Ser Asp Lys Asp Ser Phe Gln Pro 
385                 390                 395                 400 
Ala Leu Leu Gln Gly Ile Gln Thr Ile Asp Asn Ile Val Glu Gly Pro 
                405                 410                 415     
Thr Asp Tyr Glu Val Phe Ser Gln Pro Thr Phe Asp Trp Asp Thr Tyr 
            420                 425                 430         
Asn Ser 
        

<210> 4
<211> 2496
<212> DNA
<213> Solanum lycopersicum


<400> 4
atgatccaat ccagcttctc tgcagactca ccttccatgg cagccaattc cactttctct      60

cctccgccgg ccgccggtga cggagacttc aattacgacg tcgcttggta tggtaacatc     120

cagtacctcc tcaatatctc cgccattgga gctttgactt gccttcttat tttcatcttc     180

gggaagcttc gaagcgacca ccgtcgcatg cccggtccca ctgccattgt ctccaagctc     240

ttagctgcct ggcacgccac tggtgttgaa atcgcccgcc actgcggggc tgacgctgct     300

caatatctcc ttattgaggg cggcagctct gccctgctat tattcctcgc ccttctttct     360

cttgctgtaa tgctgccgtt gaatatatat gctggtaagg ctcctatggc tgatcagttt     420

tcaaagacta caataaacca tatagaaaaa ggttctccat tactctggat tcactttata     480

tttgttgtta ttgttgttgt tttggtacat tatggtataa gtgaaataca agaaaggttg     540

aaaattacta gacttagaga cggctatgga aatccgagta attctggtac aaatgtcagt     600

gcaatttttt ccattatggt gcagggtgta cctaagacct taggttttga taagacacct     660

ttagtggagt attttcagca taaatatccg gggaaggtgt atagagtagt tgtccctatg     720

gatttgtgtg ctctggatga tttagctaca gagttggtga aggttcggga agatatctct     780

aaactagtct caagaattga gttacggggt tatttgaatg agggtgagga agacgagtat     840

aataatgata gtgtgaacgg gcggggcttg ttagaacgac tgtgcttttt gtggagaaag     900

gctaaggata catggtatca tgttgtggat caattaggtt tctcagatga agagagatta     960

agaaaattgc aagagttgag agctgatttg gagatggaaa tggcatctta taaagaaggg    1020

agggcaagag gtgctggtgt agcttttgtg gtatttaagg acgtattcac agctaataag    1080

gctgtccagg acctccggaa tgagaagagg aggcgatatg gtcgattctt ctcagtcatt    1140

gagttgcaac tacagaggaa ccagtggaaa gtggagagag ctcctttagc tactgacata    1200

tactggaacc acctgggatc aacaaagttc tccttaaagc tgcgcagagt gttggtgaac    1260

acatgcctat tgttgatgtt gctattctgc agttctccac tagctgtgat tagtgctatt    1320

caaagtgcag ggagaataat caatgctgaa gctatggatc atgctcagat gtggctgaac    1380

tgggtgcagg gctcgagctg gctagcaaca ataatatttc aatttttgcc caatgttctg    1440

atttttgtga gcatgtacat tgttgtccct tcagttcttt cttatctttc taaatttgaa    1500

caacatctta ctgtatctgg tgagcaaagg gctgagctac tgaaaatggt ttgcttcttt    1560

ctggtaaatc tcattctgct tagggctctg gtcgaatcta ctcttgaggg tgctctctta    1620

agtatgggtc ggtgttattt ggatggagaa gattgcaaaa agatcgagca gtacatgact    1680

gcttcctttt tgacaaggac atgcctctcg tctcttgcat ttttaattac aagcagtttt    1740

ttgggtatat cttttgattt attagctcca attccttgga ttaagaagaa gcttcaaaag    1800

ttccgtaaaa atgacatgct tcagttggta ccagaacgga gtgaggagta cccattggaa    1860

aatcaagaca ttgatagttt ggagaggcct ctgattcatg aaaggagttc aactgtgatt    1920

gctgacaaca atggattttt acacgatgcc tctccaaatg aaattgattt ccctggacaa    1980

gatttgtctg aataccctcc agtcagccga acctcaccag ttccaaagcc gaagtttgat    2040

tttgcacagt attatgcttt caatctgaca atatttgccc taaccctgat ctattgttcg    2100

tttgctcctc tggtggttcc tgttggtgca gtttactttg ggtaccggta tttagttgac    2160

aagtacaact tcctgtttgt atacagagtg cgaggtttcc ctgctggtaa tgatgggagg    2220

ttgatggata ctgtattatc tatcatgagg ttttgtgttg acttgttcct cctttcaatg    2280

ctacttttct tttctgtacg aggagactca actaagcttc aagccatatt cacacttgga    2340

ttgttagtgg tgtataaact cttgccctct gataaggatt cttttcagcc agcgttatta    2400

caaggcatac agactattga caacattgtc gaagggccaa ctgattatga ggttttctca    2460

caacctacat ttgattggga tacgtataat tcatga                              2496


<210> 5
<211> 2496
<212> DNA
<213> Artificial Sequence


<220> 
<223> EMS mutant

<400> 5
atgatccaat ccagcttctc tgcagactca ccttccatgg cagccaattc cactttctct      60

cctccgccgg ccgccggtga cggagacttc aattacgacg tcgcttggta tggtaacatc     120

cagtacctcc tcaatatctc cgccattgga gctttgactt gccttcttat tttcatcttc     180

gggaagcttc gaagcgacca ccgtcgcatg cccggtccca ctgccattgt ctccaagctc     240

ttagctgcct ggcacgccac tggtgttgaa atcgcccgcc actgcggggc tgacgctgct     300

caatatctcc ttattgaggg cggcagctct gccctgctat tattcctcgc ccttctttct     360

cttgctgtaa tgctgccgtt gaatatatat gctggtaagg ctcctatggc tgatcagttt     420

tcaaagacta caataaacca tatagaaaaa ggttctccat tactctggat tcactttata     480

tttgttgtta ttgttgttgt tttggtacat tatggtataa gtgaaataca agaaaggttg     540

aaaattacta gacttagaga cggctatgga aatccgagta attctggtac aaatgtcagt     600

gcaatttttt ccattatggt gcagggtgta cctaagacct taggttttga taagacacct     660

ttagtggagt attttcagca taaatatccg gggaaggtgt atagagtagt tgtccctatg     720

gatttgtgtg ctctggatga tttagctaca gagttggtga aggttcggga agatatctct     780

aaactagtct caagaattga gttacggggt tatttgaatg agggtgagga agacgagtat     840

aataatgata gtgtgaacgg gcggggcttg ttagaacgac tgtgcttttt gtggagaaag     900

gctaaggata catggtatca tgttgtggat caattaggtt tctcagatga agagagatta     960

agaaaattgc aagagttgag agctgatttg gagatggaaa tggcatctta taaagaaggg    1020

agggcaagag gtgctggtgt agcttttgtg gtatttaagg acgtattcac agctaataag    1080

gctgtccagg acctccggaa tgagaagagg aggcgatatg gtcgattctt ctcagtcatt    1140

gagttgcaac tacagaggaa ccagtggaaa gtggagagag ctcctttagc tactgacata    1200

tactggaacc acctgggatc aacaaagttc tccttaaagc tgcgcagagt gttggtgaac    1260

acatgcctat tgttgatgtt gctattctgc agttctccac tagctgtgat tagtgctatt    1320

caaagtgcag ggagaataat caatgctgaa gctatggatc atgctcagat gtggctgaac    1380

tgggtgcagg gctcgagctg gctagcaaca ataatatttc aatttttgcc caatgttctg    1440

atttttgtga gcatgtacat tgttgtccct tcagttcttt cttatctttc taaatttgaa    1500

caacatctta ctgtatctgg tgagcaaagg gctgagctac tgaaaatggt ttgcttcttt    1560

ctggtaaatc tcattctgct tagggctctg gtcgaatcta ctcttgaggg tgctctctta    1620

agtatgggtc ggtgttattt ggatggagaa gattgcaaat agatcgagca gtacatgact    1680

gcttcctttt tgacaaggac atgcctctcg tctcttgcat ttttaattac aagcagtttt    1740

ttgggtatat cttttgattt attagctcca attccttgga ttaagaagaa gcttcaaaag    1800

ttccgtaaaa atgacatgct tcagttggta ccagaacgga gtgaggagta cccattggaa    1860

aatcaagaca ttgatagttt ggagaggcct ctgattcatg aaaggagttc aactgtgatt    1920

gctgacaaca atggattttt acacgatgcc tctccaaatg aaattgattt ccctggacaa    1980

gatttgtctg aataccctcc agtcagccga acctcaccag ttccaaagcc gaagtttgat    2040

tttgcacagt attatgcttt caatctgaca atatttgccc taaccctgat ctattgttcg    2100

tttgctcctc tggtggttcc tgttggtgca gtttactttg ggtaccggta tttagttgac    2160

aagtacaact tcctgtttgt atacagagtg cgaggtttcc ctgctggtaa tgatgggagg    2220

ttgatggata ctgtattatc tatcatgagg ttttgtgttg acttgttcct cctttcaatg    2280

ctacttttct tttctgtacg aggagactca actaagcttc aagccatatt cacacttgga    2340

ttgttagtgg tgtataaact cttgccctct gataaggatt cttttcagcc agcgttatta    2400

caaggcatac agactattga caacattgtc gaagggccaa ctgattatga ggttttctca    2460

caacctacat ttgattggga tacgtataat tcatga                              2496


<210> 6
<211> 1305
<212> DNA
<213> Artificial Sequence


<220> 
<223> CRISPR/Cas9 mutant

<400> 6
atgatccaat ccagcttctc tgcagactca caaaagttct ccttaaagct gcgcagagtg      60

ttggtgaaca catgcctatt gttgatgttg ctattctgca gttctccact agctgtgatt     120

agtgctattc aaagtgcagg gagaataatc aatgctgaag ctatggatca tgctcagatg     180

tggctgaact gggtgcaggg ctcgagctgg ctagcaacaa taatatttca atttttgccc     240

aatgttctga tttttgtgag catgtacatt gttgtccctt cagttctttc ttatctttct     300

aaatttgaac aacatcttac tgtatctggt gagcaaaggg ctgagctact gaaaatggtt     360

tgcttctttc tggtaaatct cattctgctt agggctctgg tcgaatctac tcttgagggt     420

gctctcttaa gtatgggtcg gtgttatttg gatggagaag attgcaaaaa gatcgagcag     480

tacatgactg cttccttttt gacaaggaca tgcctctcgt ctcttgcatt tttaattaca     540

agcagttttt tgggtatatc ttttgattta ttagctccaa ttccttggat taagaagaag     600

cttcaaaagt tccgtaaaaa tgacatgctt cagttggtac cagaacggag tgaggagtac     660

ccattggaaa atcaagacat tgatagtttg gagaggcctc tgattcatga aaggagttca     720

actgtgattg ctgacaacaa tggattttta cacgatgcct ctccaaatga aattgatttc     780

cctggacaag atttgtctga ataccctcca gtcagccgaa cctcaccagt tccaaagccg     840

aagtttgatt ttgcacagta ttatgctttc aatctgacaa tatttgccct aaccctgatc     900

tattgttcgt ttgctcctct ggtggttcct gttggtgcag tttactttgg gtaccggtat     960

ttagttgaca agtacaactt cctgtttgta tacagagtgc gaggtttccc tgctggtaat    1020

gatgggaggt tgatggatac tgtattatct atcatgaggt tttgtgttga cttgttcctc    1080

ctttcaatgc tacttttctt ttctgtacga ggagactcaa ctaagcttca agccatattc    1140

acacttggat tgttagtggt gtataaactc ttgccctctg ataaggattc ttttcagcca    1200

gcgttattac aaggcataca gactattgac aacattgtcg aagggccaac tgattatgag    1260

gttttctcac aacctacatt tgattgggat acgtataatt catga                    1305


<210> 7
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> guide RNA

<400> 7
acttcaatta cgacgtcgct                                                20


<210> 8
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Guide RNA

<400> 8
cagagctgcc gccctcaata                                                20


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence


<220> 
<223> Guide RNA

<400> 9
ataaggctgt ccaggacctc                                                20


