                         SEQUENCE LISTING

<110>  Lygos, Inc.
 
<120>  METHOD FOR PREPARATION OF MALONIC ACID

<130>  LYGOS-0025-01-US

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  500
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  1

Met Leu Arg Asn Thr Leu Lys Cys Ala Gln Leu Ser Ser Lys Tyr Gly 
1               5                   10                  15      


Phe Lys Thr Thr Thr Arg Thr Phe Met Thr Thr Gln Pro Gln Leu Asn 
            20                  25                  30          


Val Thr Asp Ala Pro Pro Val Leu Phe Thr Val Gln Asp Thr Ala Arg 
        35                  40                  45              


Val Ile Thr Leu Asn Arg Pro Lys Lys Leu Asn Ala Leu Asn Ala Glu 
    50                  55                  60                  


Met Ser Glu Ser Met Phe Lys Thr Leu Asn Glu Tyr Ala Lys Ser Asp 
65                  70                  75                  80  


Thr Thr Asn Leu Val Ile Leu Lys Ser Ser Asn Arg Pro Arg Ser Phe 
                85                  90                  95      


Cys Ala Gly Gly Asp Val Ala Thr Val Ala Ile Phe Asn Phe Asn Lys 
            100                 105                 110         


Glu Phe Ala Lys Ser Ile Lys Phe Phe Thr Asp Glu Tyr Ser Leu Asn 
        115                 120                 125             


Phe Gln Ile Ala Thr Tyr Leu Lys Pro Ile Val Thr Phe Met Asp Gly 
    130                 135                 140                 


Ile Thr Met Gly Gly Gly Val Gly Leu Ser Ile His Thr Pro Phe Arg 
145                 150                 155                 160 


Ile Ala Thr Glu Asn Thr Lys Trp Ala Met Pro Glu Met Asp Ile Gly 
                165                 170                 175     


Phe Phe Pro Asp Val Gly Ser Thr Phe Ala Leu Pro Arg Ile Val Thr 
            180                 185                 190         


Leu Ala Asn Ser Asn Ser Gln Met Ala Leu Tyr Leu Cys Leu Thr Gly 
        195                 200                 205             


Glu Val Val Thr Gly Ala Asp Ala Tyr Met Leu Gly Leu Ala Ser His 
    210                 215                 220                 


Tyr Val Ser Ser Glu Asn Leu Asp Ala Leu Gln Lys Arg Leu Gly Glu 
225                 230                 235                 240 


Ile Ser Pro Pro Phe Asn Asn Asp Pro Gln Ser Ala Tyr Phe Phe Gly 
                245                 250                 255     


Met Val Asn Glu Ser Ile Asp Glu Phe Val Ser Pro Leu Pro Lys Asp 
            260                 265                 270         


Tyr Val Phe Lys Tyr Ser Asn Glu Lys Leu Asn Val Ile Glu Ala Cys 
        275                 280                 285             


Phe Asn Leu Ser Lys Asn Gly Thr Ile Glu Asp Ile Met Asn Asn Leu 
    290                 295                 300                 


Arg Gln Tyr Glu Gly Ser Ala Glu Gly Lys Ala Phe Ala Gln Glu Ile 
305                 310                 315                 320 


Lys Thr Lys Leu Leu Thr Lys Ser Pro Ser Ser Leu Gln Ile Ala Leu 
                325                 330                 335     


Arg Leu Val Gln Glu Asn Ser Arg Asp His Ile Glu Ser Ala Ile Lys 
            340                 345                 350         


Arg Asp Leu Tyr Thr Ala Ala Asn Met Cys Met Asn Gln Asp Ser Leu 
        355                 360                 365             


Val Glu Phe Ser Glu Ala Thr Lys His Lys Leu Ile Asp Lys Gln Arg 
    370                 375                 380                 


Val Pro Tyr Pro Trp Thr Lys Lys Glu Gln Leu Phe Val Ser Gln Leu 
385                 390                 395                 400 


Thr Ser Ile Thr Ser Pro Lys Pro Ser Leu Pro Met Ser Leu Leu Arg 
                405                 410                 415     


Asn Thr Ser Asn Val Thr Trp Thr Gln Tyr Pro Tyr His Ser Lys Tyr 
            420                 425                 430         


Gln Leu Pro Thr Glu Gln Glu Ile Ala Ala Tyr Ile Glu Lys Arg Thr 
        435                 440                 445             


Asn Asp Asp Thr Gly Ala Lys Val Thr Glu Arg Glu Val Leu Asn His 
    450                 455                 460                 


Phe Ala Asn Val Ile Pro Ser Arg Arg Gly Lys Leu Gly Ile Gln Ser 
465                 470                 475                 480 


Leu Cys Lys Ile Val Cys Glu Arg Lys Cys Glu Glu Val Asn Asp Gly 
                485                 490                 495     


Leu Arg Trp Lys 
            500 


<210>  2
<211>  1000
<212>  DNA
<213>  Haemophilus influenzae

<400>  2
atgttttaca ctgaaactta tgatgtgatt gtgatcggtg gtggtcatgc gggtacagaa       60

gccgcacttg caccagctcg tatgggattt aaaacccttt tattaacaca taatgtagat      120

actttagggc aaatgtcttg taaccctgca attggtggga tcggtaaagg tcatttagta      180

aaagaagtag atgcaatggg cggtttaatg gcgcatgctg cagataaagc agggatccaa      240

tttcgtactt taaatagcag taaaggccca gcagtgcgtg ctactcgagc tcaagctgac      300

agagttctat atcgtcaagc tgttcgtact gcattagaaa atcaacctaa tttagatatt      360

ttccaacaag aagcgaccga tattctgatt aagcaagatc gagttacagg cgttagcaca      420

aaaatgggat taacttttcg tgctaaatca gtggtattaa ctgcgggtac tttcttagct      480

ggtaaaattc atattggttt ggaaaattat gaaggtggcc gtgcagggga tcctgcttct      540

gtaaatcttt cacatcgatt aagagatctc ggattacgtg tagatcgcct taaaacaggt      600

acaccgccgc gtattgatgc acgtacgatc aattttgata ttttagctaa acaacacggt      660

gatgctgttt tacctgtgtt ttcttttatg ggatcagttg atgatcaccc tcaacaaatt      720

ccttgttata taactcatac caatgaacaa acccatgaag tgatccgtaa taacttggat      780

cgcagtccaa tgtatactgg tgtgattgaa gggatcggtc cacgttattg cccatccatt      840

gaagataaag tgatgcgttt ctcggatcgt aattcacatc aaatttattt agaaccagaa      900

ggcttaacca gtaatgaagt gtatccaaac gggatctcta ccagtttacc gtttgacgtg      960

caaatgggca ttgtgaattc tatgaaaggt ttagaaaacg                           1000


<210>  3
<211>  598
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  EHD3 EC https://protect-us.mimecast.com/s/OOiwCJ6AkKTVxRWAiVil7A?domain=3.1.2.4 malonyl-CoA hydrolase consensus sequence


<220>
<221>  VARIANT
<222>  (1)..(16)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (19)..(19)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (20)..(21)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (22)..(22)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (23)..(23)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (27)..(27)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (28)..(28)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (29)..(30)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (32)..(35)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (37)..(40)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (44)..(44)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (45)..(46)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (47)..(47)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (48)..(48)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (49)..(52)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (53)..(53)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (54)..(58)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (59)..(59)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (60)..(60)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (61)..(61)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (62)..(62)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (63)..(65)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (66)..(90)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (95)..(97)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (98)..(100)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (104)..(104)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (107)..(107)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (108)..(108)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (111)..(112)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (113)..(113)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (118)..(118)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (125)..(125)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (126)..(126)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (129)..(129)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (130)..(130)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (131)..(131)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (133)..(133)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (134)..(134)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (139)..(139)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (142)..(142)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (143)..(144)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (146)..(149)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (150)..(150)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (152)..(152)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (157)..(157)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (158)..(158)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (166)..(166)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (167)..(167)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (169)..(169)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (172)..(172)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (173)..(173)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (174)..(175)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (177)..(178)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (179)..(180)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (181)..(181)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (182)..(182)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (183)..(183)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (186)..(186)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (187)..(187)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (188)..(188)
<223>  Ala, Asp, Arg, His, Lys, Ser, Thr, Asn, Gln, Tyr

<220>
<221>  VARIANT
<222>  (191)..(191)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (195)..(195)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (199)..(199)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (202)..(205)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (207)..(207)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (217)..(217)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (218)..(218)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (219)..(219)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (221)..(221)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (225)..(225)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (231)..(231)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (247)..(248)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (251)..(251)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (253)..(253)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (254)..(255)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (256)..(256)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (257)..(257)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (260)..(260)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (261)..(261)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (262)..(262)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (264)..(264)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (266)..(266)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (270)..(270)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (273)..(273)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (274)..(275)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (276)..(276)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (278)..(279)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (282)..(283)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (285)..(285)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (290)..(290)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (291)..(291)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (292)..(292)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (293)..(293)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (295)..(295)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (296)..(297)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (299)..(299)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (300)..(300)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (305)..(305)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (306)..(306)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (308)..(308)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (309)..(309)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (310)..(310)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (311)..(311)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (312)..(312)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (313)..(313)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (314)..(314)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (315)..(317)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (318)..(318)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (320)..(320)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (321)..(322)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (325)..(325)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (326)..(327)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (329)..(329)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (330)..(330)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (331)..(331)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (332)..(332)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (335)..(336)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (338)..(338)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (339)..(339)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (341)..(341)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (342)..(342)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (344)..(344)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (346)..(346)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (348)..(348)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (350)..(350)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (351)..(351)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (353)..(353)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (356)..(356)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (357)..(357)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (360)..(360)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (361)..(361)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (362)..(362)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (363)..(363)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (364)..(364)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (365)..(366)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (367)..(367)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (368)..(368)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (369)..(369)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (370)..(371)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (372)..(373)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (375)..(376)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (377)..(377)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (378)..(378)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (379)..(380)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (381)..(381)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (383)..(383)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (385)..(385)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (386)..(387)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (389)..(389)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (390)..(390)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (393)..(393)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (394)..(394)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (395)..(395)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (396)..(396)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (397)..(397)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (398)..(398)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (400)..(400)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (401)..(401)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (405)..(405)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (407)..(407)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (409)..(409)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (411)..(411)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (412)..(412)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (413)..(414)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (415)..(415)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (416)..(416)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (418)..(418)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (419)..(419)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (420)..(420)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (421)..(421)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (422)..(422)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (423)..(423)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (424)..(424)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (426)..(426)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (427)..(428)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (431)..(431)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (434)..(434)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (438)..(438)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (440)..(440)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (441)..(443)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (445)..(445)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (446)..(446)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (447)..(448)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (451)..(451)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (452)..(452)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (454)..(454)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (455)..(456)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (459)..(459)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (460)..(460)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (463)..(463)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (464)..(464)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (464)..(464)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (469)..(471)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (472)..(472)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (473)..(474)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (475)..(475)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (476)..(476)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (477)..(477)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (478)..(478)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (480)..(480)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (481)..(482)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (483)..(484)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (485)..(485)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (487)..(487)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (489)..(489)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (490)..(490)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (492)..(492)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (493)..(493)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (495)..(495)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (496)..(496)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (498)..(499)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (501)..(501)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (504)..(504)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (505)..(505)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (508)..(509)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (510)..(510)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (511)..(511)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (516)..(516)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (517)..(517)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (518)..(518)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (519)..(519)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (520)..(520)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (521)..(521)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (524)..(524)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (525)..(527)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (528)..(528)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (530)..(530)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (532)..(534)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (535)..(535)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (537)..(537)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (538)..(539)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (540)..(540)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (541)..(541)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (542)..(542)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (543)..(543)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (543)..(543)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (544)..(545)
<223>  Arg, Lys, or His

<220>
<221>  VARIANT
<222>  (546)..(546)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (547)..(548)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (549)..(550)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (552)..(552)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (554)..(554)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (556)..(556)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (557)..(557)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (558)..(559)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (560)..(560)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (562)..(562)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (564)..(564)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (565)..(565)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (566)..(566)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (567)..(568)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (569)..(569)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (570)..(572)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (573)..(573)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (574)..(575)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (577)..(577)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (578)..(580)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (582)..(582)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (585)..(585)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (586)..(586)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (588)..(588)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (589)..(590)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (591)..(591)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (592)..(592)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (593)..(598)
<223>  Xaa can be any naturally occurring amino acid or absent

<400>  3

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Met Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Gln Xaa Xaa Xaa Xaa Xaa Xaa 
            20                  25                  30          


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gly Phe Xaa Xaa Xaa Xaa Xaa 
        35                  40                  45              


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    50                  55                  60                  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
65                  70                  75                  80  


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Gln Xaa Asn Xaa Xaa 
                85                  90                  95      


Xaa Xaa Xaa Xaa Val Xaa Phe Xaa Xaa Gln Xaa Xaa Ala Arg Xaa Xaa 
            100                 105                 110         


Xaa Leu Asn Arg Pro Xaa Lys Leu Asn Ala Leu Asn Xaa Xaa Met Xaa 
        115                 120                 125             


Xaa Xaa Xaa Phe Xaa Xaa Leu Asn Glu Tyr Xaa Lys Ser Xaa Xaa Xaa 
    130                 135                 140                 


Asn Xaa Xaa Xaa Xaa Xaa Ser Xaa Asn Gln Pro Arg Xaa Xaa Cys Ala 
145                 150                 155                 160 


Gly Gly Asp Val Ala Xaa Xaa Ala Xaa Xaa Asn Xaa Xaa Xaa Xaa Phe 
                165                 170                 175     


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Phe Phe Xaa Xaa Xaa Tyr Ser Xaa Asn 
            180                 185                 190         


Phe Gln Xaa Ala Thr Tyr Xaa Lys Pro Xaa Xaa Xaa Xaa Met Xaa Gly 
        195                 200                 205             


Ile Thr Met Gly Gly Gly Val Gly Xaa Xaa Xaa His Xaa Pro Phe Arg 
    210                 215                 220                 


Xaa Ala Thr Glu Asn Thr Xaa Trp Ala Met Pro Glu Met Asp Ile Gly 
225                 230                 235                 240 


Phe Phe Pro Asp Val Gly Xaa Xaa Phe Ala Xaa Pro Xaa Xaa Xaa Xaa 
                245                 250                 255     


Xaa Ala Asn Xaa Xaa Xaa Gln Xaa Ala Xaa Tyr Leu Cys Xaa Thr Gly 
            260                 265                 270         


Xaa Xaa Xaa Xaa Gly Xaa Xaa Ala Tyr Xaa Xaa Gly Xaa Ala Ser His 
        275                 280                 285             


Tyr Xaa Xaa Xaa Xaa Asn Xaa Xaa Xaa Leu Xaa Xaa Arg Leu Gly Glu 
    290                 295                 300                 


Xaa Xaa Pro Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gln Xaa 
305                 310                 315                 320 


Xaa Xaa Phe Phe Xaa Xaa Xaa Asn Xaa Xaa Xaa Xaa Glu Phe Xaa Xaa 
                325                 330                 335     


Pro Xaa Xaa Pro Xaa Xaa Tyr Xaa Phe Xaa Tyr Xaa Asn Xaa Xaa Leu 
            340                 345                 350         


Xaa Val Ile Xaa Xaa Cys Phe Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        355                 360                 365             


Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Tyr Xaa Gly 
    370                 375                 380                 


Xaa Xaa Xaa Ala Xaa Xaa Phe Ala Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa 
385                 390                 395                 400 


Xaa Lys Ser Pro Xaa Ser Xaa Gln Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa 
                405                 410                 415     


Asn Xaa Xaa Xaa Xaa Xaa Xaa Xaa Ala Xaa Xaa Xaa Asp Leu Xaa Thr 
            420                 425                 430         


Ala Xaa Asn Met Cys Xaa Asn Xaa Xaa Xaa Xaa Gln Xaa Xaa Xaa Xaa 
        435                 440                 445             


Glu Phe Xaa Xaa Ala Xaa Xaa Xaa Lys Leu Xaa Xaa Lys Gln Xaa Xaa 
    450                 455                 460                 


Pro Tyr Pro Trp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Gln Xaa 
465                 470                 475                 480 


Xaa Xaa Xaa Xaa Xaa Pro Xaa Pro Xaa Xaa Pro Xaa Xaa Leu Xaa Xaa 
                485                 490                 495     


Asn Xaa Xaa Asn Xaa Thr Trp Xaa Xaa Tyr Pro Xaa Xaa Xaa Xaa Tyr 
            500                 505                 510         


Gln Leu Pro Xaa Xaa Xaa Xaa Xaa Xaa Gln Tyr Xaa Xaa Xaa Xaa Xaa 
        515                 520                 525             


Asn Xaa Asn Xaa Xaa Xaa Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    530                 535                 540                 


Xaa Xaa Xaa Xaa Xaa Xaa Phe Xaa Asn Xaa Asn Xaa Xaa Xaa Xaa Xaa 
545                 550                 555                 560 


Lys Xaa Gly Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Cys 
                565                 570                 575     


Xaa Xaa Xaa Xaa Ala Xaa Gly Gly Xaa Xaa Trp Xaa Xaa Xaa Xaa Xaa 
            580                 585                 590         


Xaa Xaa Xaa Xaa Xaa Xaa 
        595             


<210>  4
<211>  351
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Bacillus EC https://protect-us.mimecast.com/s/OOiwCJ6AkKTVxRWAiVil7A?domain=3.1.2.4 malonyl-CoA hydrolase consensus sequence


<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (9)..(9)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (10)..(10)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (16)..(16)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (45)..(45)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (46)..(47)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (48)..(48)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (51)..(51)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (59)..(59)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (79)..(79)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (86)..(86)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (91)..(91)
<223>  Ala, Asp, Arg, His, Lys, Ser, Thr, Asn, Gln, Tyr

<220>
<221>  VARIANT
<222>  (93)..(93)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (98)..(98)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (125)..(125)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (131)..(131)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (156)..(156)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (161)..(161)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (163)..(163)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (168)..(168)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (171)..(171)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (175)..(175)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (188)..(189)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (190)..(190)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (191)..(191)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (194)..(194)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (197)..(197)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (198)..(198)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (199)..(199)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (200)..(200)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (200)..(200)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (201)..(201)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (202)..(202)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (205)..(206)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (207)..(209)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (211)..(211)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (212)..(212)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (213)..(213)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (216)..(216)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (217)..(218)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (219)..(219)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (220)..(220)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (223)..(226)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (227)..(227)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (228)..(228)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (229)..(229)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (230)..(230)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (232)..(233)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (234)..(235)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (237)..(237)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (238)..(238)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (240)..(240)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (245)..(245)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (246)..(246)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (247)..(247)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (249)..(249)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (252)..(252)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (256)..(256)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (257)..(257)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (259)..(260)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (263)..(263)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (264)..(264)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (265)..(265)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (266)..(266)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (267)..(267)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (268)..(268)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (275)..(275)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (285)..(285)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (286)..(286)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (288)..(288)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (289)..(289)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (292)..(292)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (294)..(294)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (301)..(301)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (310)..(310)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (319)..(319)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (321)..(321)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (332)..(332)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (334)..(334)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (335)..(335)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (338)..(338)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (339)..(341)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (344)..(344)
<223>  Arg, Lys or His

<400>  4

Met Thr Glu Xaa Val Leu Phe Ser Xaa Xaa Xaa Asn Gly Val Ala Xaa 
1               5                   10                  15      


Ile Thr Leu Asn Arg Pro Lys Ala Leu Asn Ser Leu Ser Tyr Xaa Met 
            20                  25                  30          


Leu Gln Pro Ile Gly Gln Lys Leu Lys Glu Trp Glu Xaa Xaa Xaa Xaa 
        35                  40                  45              


Ile Ala Xaa Ile Val Leu Lys Gly Ala Gly Xaa Lys Gly Phe Cys Ala 
    50                  55                  60                  


Gly Gly Asp Ile Lys Thr Leu Tyr Glu Ala Arg Ser Asn Glu Xaa Ala 
65                  70                  75                  80  


Leu Gln Xaa Ala Glu Xaa Phe Phe Xaa Glu Xaa Tyr Xaa Ile Asp Thr 
                85                  90                  95      


Tyr Xaa Tyr Gln Tyr Xaa Lys Pro Ile Ile Ala Cys Leu Asp Gly Ile 
            100                 105                 110         


Val Met Gly Gly Gly Val Gly Leu Thr Asn Gly Ala Xaa Tyr Arg Ile 
        115                 120                 125             


Val Thr Xaa Xaa Thr Lys Trp Ala Met Pro Glu Met Asn Ile Gly Phe 
    130                 135                 140                 


Phe Pro Asp Val Gly Ala Ala Tyr Phe Leu Asn Xaa Ala Pro Gly Tyr 
145                 150                 155                 160 


Xaa Gly Xaa Tyr Val Ala Leu Xaa Ala Xaa Xaa Leu Lys Ala Xaa Asp 
                165                 170                 175     


Val Leu Phe Ile Asn Ala Ala Asp Tyr Phe Met Xaa Xaa Xaa Xaa Leu 
            180                 185                 190         


Pro Xaa Phe Leu Xaa Xaa Xaa Xaa Xaa Xaa Asn Trp Xaa Xaa Xaa Xaa 
        195                 200                 205             


Xaa Val Xaa Xaa Xaa Leu Lys Xaa Xaa Xaa Xaa Xaa Phe Ala Xaa Xaa 
    210                 215                 220                 


Xaa Xaa Xaa Xaa Xaa Xaa Leu Xaa Xaa Xaa Xaa Glu Xaa Xaa Asn Xaa 
225                 230                 235                 240 


His Phe Ala Phe Xaa Xaa Xaa Glu Xaa Ile Ile Xaa Ser Leu Glu Xaa 
                245                 250                 255     


Xaa Gln Xaa Xaa Phe Ala Xaa Xaa Xaa Xaa Xaa Xaa Leu Leu Ser Lys 
            260                 265                 270         


Ser Pro Xaa Ser Leu Lys Val Thr Leu Lys Gln Phe Xaa Xaa Gly Xaa 
        275                 280                 285             


Xaa Lys Ser Xaa Glu Xaa Cys Phe Ala Thr Asp Leu Xaa Leu Ala Lys 
    290                 295                 300                 


Asn Phe Met Arg His Xaa Asp Phe Phe Glu Gly Val Arg Ser Xaa Val 
305                 310                 315                 320 


Xaa Asp Lys Asp Gln Asn Pro Asn Tyr Lys Tyr Xaa Gln Xaa Xaa Asp 
                325                 330                 335     


Val Xaa Xaa Xaa Xaa Val Asn Xaa Phe Phe Asn Leu Leu Asn Ala 
            340                 345                 350     


<210>  5
<211>  370
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Pseudomonas EC https://protect-us.mimecast.com/s/OOiwCJ6AkKTVxRWAiVil7A?domain=3.1.2.4 malonyl-CoA hydrolase consensus sequence


<220>
<221>  VARIANT
<222>  (3)..(3)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (4)..(4)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (7)..(7)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (8)..(8)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (9)..(10)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (12)..(12)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (18)..(18)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (20)..(20)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (25)..(25)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (26)..(26)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (31)..(31)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (36)..(36)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (37)..(37)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (40)..(40)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (41)..(41)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (47)..(47)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (48)..(48)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (51)..(52)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (55)..(55)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (69)..(69)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (71)..(72)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (78)..(78)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (79)..(79)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (80)..(80)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (84)..(84)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (90)..(90)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (94)..(94)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (95)..(95)
<223>  Ala, Asp, Arg, His, Lys, Ser, Thr, Asn, Gln, or Tyr

<220>
<221>  VARIANT
<222>  (99)..(99)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (100)..(100)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (101)..(101)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (104)..(104)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (109)..(110)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (116)..(116)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (118)..(118)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (125)..(125)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (129)..(129)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (130)..(130)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (136)..(137)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (144)..(144)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (145)..(145)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (159)..(159)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (161)..(161)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (164)..(164)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (169)..(169)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (172)..(172)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (175)..(175)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (176)..(176)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (179)..(179)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (182)..(182)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (185)..(185)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (191)..(191)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (192)..(192)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (193)..(193)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (194)..(194)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (197)..(198)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (200)..(200)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (201)..(202)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (204)..(204)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (206)..(206)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (212)..(212)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (213)..(213)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (214)..(214)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (217)..(217)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (220)..(220)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (221)..(221)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (223)..(224)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (225)..(225)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (226)..(226)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (228)..(228)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (229)..(230)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (231)..(231)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (233)..(233)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (236)..(236)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (242)..(242)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (243)..(243)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (245)..(245)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (246)..(246)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (249)..(249)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (251)..(251)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (252)..(252)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (254)..(254)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (257)..(257)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (259)..(259)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (260)..(260)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (262)..(262)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (263)..(263)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (264)..(264)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (265)..(265)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (266)..(266)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (270)..(270)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (272)..(272)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (275)..(276)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (277)..(277)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (278)..(278)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (279)..(279)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (283)..(283)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (284)..(284)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (286)..(286)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (287)..(287)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (290)..(290)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (292)..(292)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (293)..(293)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (297)..(297)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (302)..(302)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (303)..(303)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (305)..(305)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (309)..(309)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (310)..(310)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (319)..(319)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (321)..(321)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (329)..(329)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (331)..(331)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (333)..(333)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (336)..(336)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (338)..(338)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (341)..(341)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (342)..(342)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (345)..(345)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (347)..(347)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (349)..(350)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (350)..(350)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (351)..(351)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (355)..(355)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (356)..(356)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (357)..(357)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (357)..(357)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (359)..(359)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (361)..(362)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (364)..(364)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (364)..(364)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (366)..(366)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (367)..(367)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (367)..(367)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (368)..(368)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (369)..(370)
<223>  Xaa can be any naturally occurring amino acid

<400>  5

Met Asn Xaa Xaa Phe Glu Xaa Xaa Xaa Xaa Xaa Xaa Gly Ala Arg Ile 
1               5                   10                  15      


Gly Xaa Ala Xaa Leu Asp Ala Xaa Xaa Xaa Leu Asn Ala Leu Xaa Leu 
            20                  25                  30          


Pro Met Ile Xaa Xaa Leu Gly Xaa Xaa Xaa Xaa Ala Trp Ala Xaa Xaa 
        35                  40                  45              


Pro Gly Xaa Xaa Cys Val Xaa Leu Arg Gly Asn Gly Ala Lys Ala Phe 
    50                  55                  60                  


Cys Ala Gly Gly Xaa Val Xaa Xaa Leu Xaa Xaa Ala Cys Xaa Xaa Xaa 
65                  70                  75                  80  


Pro Gly Xaa Xaa Pro Pro Leu Ala Ala Xaa Phe Phe Ala Xaa Xaa Tyr 
                85                  90                  95      


Arg Leu Xaa Xaa Xaa Xaa His Xaa Tyr Pro Lys Pro Xaa Xaa Cys Trp 
            100                 105                 110         


Gly His Gly Xaa Val Xaa Gly Gly Gly Met Gly Leu Xaa Gln Gly Ala 
        115                 120                 125             


Xaa Xaa Arg Ile Val Thr Pro Xaa Xaa Arg Leu Ala Met Pro Glu Xaa 
    130                 135                 140                 


Xaa Ile Gly Leu Tyr Pro Asp Val Gly Ala Ser Trp Phe Leu Xaa Arg 
145                 150                 155                 160 


Xaa Pro Gly Xaa Leu Gly Leu Phe Xaa Gly Leu Xaa Gly Ala Xaa Xaa 
                165                 170                 175     


Asn Ala Xaa Asp Ala Xaa Asp Leu Xaa Leu Ala Asp Arg Phe Xaa Xaa 
            180                 185                 190         


Xaa Xaa Gln Gln Xaa Xaa Leu Xaa Xaa Xaa Leu Xaa Gln Xaa Asn Trp 
        195                 200                 205             


Gln Glu Gln Xaa Xaa Xaa Gln Leu Xaa Ser Leu Xaa Xaa Ala Xaa Xaa 
    210                 215                 220                 


Xaa Xaa Ala Xaa Xaa Xaa Xaa Pro Xaa Ala Gln Xaa Leu Pro Arg Arg 
225                 230                 235                 240 


Gln Xaa Xaa Asp Xaa Xaa Leu Asp Xaa Ala Xaa Xaa Ala Xaa Ala Trp 
                245                 250                 255     


Xaa Ala Xaa Xaa Ala Xaa Xaa Xaa Xaa Xaa Asp Pro Leu Xaa Ala Xaa 
            260                 265                 270         


Ala Ala Xaa Xaa Xaa Xaa Xaa Gly Cys Pro Xaa Xaa Ala Xaa Xaa Val 
        275                 280                 285             


Trp Xaa Gln Xaa Xaa Arg Ala Arg Xaa Leu Ser Leu Ala Xaa Xaa Phe 
    290                 295                 300                 


Xaa Met Glu Tyr Xaa Xaa Ser Leu Asn Cys Cys Arg His Pro Xaa Phe 
305                 310                 315                 320 


Xaa Glu Gly Val Arg Ala Arg Leu Xaa Asp Xaa Asp Xaa Gln Pro Xaa 
                325                 330                 335     


Trp Xaa Trp Pro Xaa Xaa Ala Gln Xaa Pro Xaa Ala Xaa Xaa Xaa Ala 
            340                 345                 350         


His Phe Xaa Xaa Xaa Trp Xaa Gly Xaa Xaa Pro Xaa Ala Xaa Xaa Xaa 
        355                 360                 365             


Xaa Xaa 
    370 


<210>  6
<211>  505
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  General bacterial EC https://protect-us.mimecast.com/s/OOiwCJ6AkKTVxRWAiVil7A?domain=3.1.2.4 malonyl-CoA hydrolase consensus 
       sequence


<220>
<221>  VARIANT
<222>  (1)..(10)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (11)..(11)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (12)..(23)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (24)..(24)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (25)..(30)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (32)..(32)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (34)..(35)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (39)..(40)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (42)..(42)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (43)..(43)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (48)..(48)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (52)..(52)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (57)..(57)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (63)..(63)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (67)..(67)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (70)..(70)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (73)..(73)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (74)..(74)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (75)..(75)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (80)..(80)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (83)..(83)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (86)..(87)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (89)..(89)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (90)..(90)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (95)..(95)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (102)..(102)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (103)..(103)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (104)..(104)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (105)..(105)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (106)..(106)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (108)..(108)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (116)..(116)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (127)..(127)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (128)..(128)
<223>  Ala, Asp, Arg, His, Lys, Ser, Thr, Asn, Glu, Tyr

<220>
<221>  VARIANT
<222>  (131)..(131)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (132)..(132)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (135)..(135)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (142)..(143)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (150)..(150)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (164)..(164)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (165)..(166)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (170)..(170)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (171)..(171)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (193)..(193)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (195)..(200)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (208)..(208)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (211)..(211)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (215)..(215)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (221)..(221)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (223)..(223)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (227)..(227)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (233)..(233)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (239)..(239)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (242)..(242)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (245)..(245)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (252)..(252)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (253)..(253)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (261)..(261)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (262)..(262)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (263)..(263)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (265)..(265)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (275)..(275)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (278)..(278)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (281)..(284)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (289)..(289)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (296)..(298)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (301)..(301)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (306)..(306)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (308)..(308)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (311)..(311)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (313)..(314)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (316)..(316)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (328)..(328)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (332)..(332)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (334)..(334)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (335)..(335)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (337)..(337)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (338)..(338)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (350)..(350)
<223>  Ser or Thr

<220>
<221>  VARIANT
<222>  (351)..(351)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (353)..(353)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (358)..(358)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (361)..(361)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (367)..(368)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (369)..(372)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (374)..(374)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (379)..(379)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (380)..(380)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (383)..(384)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (386)..(386)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (387)..(387)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (393)..(393)
<223>  Arg, Lys or His

<220>
<221>  VARIANT
<222>  (397)..(397)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (400)..(400)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (402)..(402)
<223>  Asp or Glu

<220>
<221>  VARIANT
<222>  (405)..(419)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (420)..(420)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (427)..(427)
<223>  Ile, Leu or Val

<220>
<221>  VARIANT
<222>  (431)..(431)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (433)..(434)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (440)..(440)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (441)..(446)
<223>  Xaa can be any naturally occurring amino acid or absent

<220>
<221>  VARIANT
<222>  (447)..(448)
<223>  Xaa can be any naturally occurring amino acid

<220>
<221>  VARIANT
<222>  (449)..(505)
<223>  Xaa can be any naturally occurring amino acid or absent

<400>  6

Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
1               5                   10                  15      


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Met Xaa 
            20                  25                  30          


Met Xaa Xaa Thr Glu His Xaa Xaa Phe Xaa Xaa Ser Glu Asn Gly Xaa 
        35                  40                  45              


Ala Ser Ile Xaa Leu Asn Arg Pro Xaa Ala Leu Asn Ser Leu Xaa Tyr 
    50                  55                  60                  


Asp Met Xaa Gln Pro Xaa Gly Gln Xaa Xaa Xaa Glu Trp Glu Asn Xaa 
65                  70                  75                  80  


Glu Arg Xaa Ala Leu Xaa Xaa Leu Xaa Xaa Gly Ala Gly Thr Xaa Gly 
                85                  90                  95      


Phe Cys Ala Gly Gly Xaa Xaa Xaa Xaa Xaa Tyr Xaa Ala Arg Ser Asn 
            100                 105                 110         


Glu Pro Gly Xaa Ala Leu Gln His Ala Glu Arg Phe Phe Glu Xaa Xaa 
        115                 120                 125             


Tyr Glu Xaa Xaa Thr Tyr Xaa Tyr Gln Tyr Lys Lys Pro Xaa Xaa Ala 
    130                 135                 140                 


Cys Leu Asp Gly Ile Xaa Met Gly Gly Gly Val Gly Leu Thr Asn Gly 
145                 150                 155                 160 


Ala Lys Tyr Xaa Xaa Xaa Thr Glu Arg Xaa Xaa Trp Ala Met Pro Glu 
                165                 170                 175     


Met Asn Ile Gly Phe Phe Pro Asp Val Gly Ala Ala Tyr Phe Leu Asn 
            180                 185                 190         


Xaa Ala Xaa Xaa Xaa Xaa Xaa Xaa Pro Gly Tyr Leu Gly Arg Tyr Xaa 
        195                 200                 205             


Ala Leu Xaa Ala Ser Ile Xaa Lys Ala Ser Asp Val Xaa Phe Xaa Asn 
    210                 215                 220                 


Ala Ala Xaa Tyr Phe Met Thr Ser Xaa Ser Leu Pro Ala Phe Xaa Thr 
225                 230                 235                 240 


Glu Xaa Glu Ser Xaa Asn Trp His Lys Glu Asp Xaa Xaa His Thr His 
                245                 250                 255     


Leu Leu Lys Glu Xaa Xaa Xaa Val Xaa Arg Thr Phe Ala Thr Ala Pro 
            260                 265                 270         


Asn Leu Xaa Ser Glu Xaa Ala Pro Xaa Xaa Xaa Xaa Ser Leu Glu Glu 
        275                 280                 285             


Xaa Asn Ser His Phe Ala Phe Xaa Xaa Xaa Asp Thr Xaa Glu Glu Ile 
    290                 295                 300                 


Trp Xaa Ala Xaa His Ser Xaa Glu Xaa Xaa Lys Xaa Gln Ser Ser Phe 
305                 310                 315                 320 


Ala Leu Lys Thr Lys Glu Thr Xaa Leu Ser Lys Xaa Pro Xaa Xaa Leu 
                325                 330                 335     


Xaa Xaa Thr Leu Lys Gln Phe Ile Asp Gly Arg Asp Lys Xaa Xaa Glu 
            340                 345                 350         


Xaa Cys Phe Ala Thr Xaa Leu Val Xaa Ala Lys Asn Phe Met Xaa Xaa 
        355                 360                 365             


Xaa Xaa Xaa Xaa Glu Xaa Phe Phe Glu Gly Xaa Xaa Ser Val Xaa Xaa 
    370                 375                 380                 


Asp Xaa Xaa Gln Asn Pro Asn Tyr Xaa Tyr Lys Gln Xaa Ser Asp Xaa 
385                 390                 395                 400 


Ser Xaa Glu Asp Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                405                 410                 415     


Xaa Xaa Xaa Xaa Asn Arg Phe Phe Asn Leu Xaa Asn Ala Gly Xaa His 
            420                 425                 430         


Xaa Xaa Pro Leu Ala Asp Leu Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
        435                 440                 445             


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
    450                 455                 460                 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
465                 470                 475                 480 


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
                485                 490                 495     


Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa Xaa 
            500                 505 


<210>  7
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A93

<400>  7
ccaatatata ataaaatatg gaggaatgcg atgctcagaa atacgctaaa atgtgcccaa       60


<210>  8
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A94

<400>  8
tgcctggaga tccttactcg agttggatcc ttatttccat cttaagccat cgttaacttc       60


<210>  9
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A95

<400>  9
ttttactgat gcgtattctt tgaattttca aatagca                                37


<210>  10
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A96

<400>  10
tcaaagaata cgcatcagta aaaaatttga tgga                                   34


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A97

<400>  11
ttttactgat gtttattctt tgaattttca aatagcaact t                           41


<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A98

<400>  12
tcaaagaata aacatcagta aaaaatttga tggacttgg                              39


<210>  13
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A99

<400>  13
ttttactgat tcgtattctt tgaattttca aatagcaac                              39


<210>  14
<211>  36
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A100

<400>  14
tcaaagaata cgaatcagta aaaaatttga tggact                                 36


<210>  15
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (15)..(17)
<223>  n is a, c, g, or t

<400>  15
aattttttac tgatnnntat tctttgaatt ttcaaatagc                             40


<210>  16
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer


<220>
<221>  misc_feature
<222>  (13)..(15)
<223>  n is a, c, g, or t

<400>  16
ttcaaagaat aannnatcag taaaaaattt gatggacttg                             40


<210>  17
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A120

<400>  17
ccaatatata ataaaatatg gaggaatgcg atgtctacaa cacataacgt ccctc            55


<210>  18
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer A121

<400>  18
tgcctggaga tccttactcg agttggatcc ttactcaaca ggtaaggcgc gag              53


<210>  19
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Y1-11_A13-R

<400>  19
ctaattacat gactcgaggt cgacggtatc gttatttcca tcttaagcca tcgttaactt       60

c                                                                       61


<210>  20
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer Y1-11_A13-F

<400>  20
cattagaaag aaagcatagc aatctaatct aagtttaaaa caatgactac tcaaccccag       60

ctaaatg                                                                 67


<210>  21
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO012

<400>  21
gaaagcatag caatctaatc taagtttaaa acaatgaccg aacaagtctt attctcagta       60


<210>  22
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO013

<400>  22
ctaattacat gactcgaggt cgacggtatc gttaagcgtt caacaaattg aaaaatctg        59


<210>  23
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO014

<400>  23
gaaagcatag caatctaatc taagtttaaa acaatgaccg aacatgtatt attctcag         58


<210>  24
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO015

<400>  24
ctaattacat gactcgaggt cgacggtatc gttaagcgtt taacaaattg aaaaatc          57


<210>  25
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO016

<400>  25
gaaagcatag caatctaatc taagtttaaa acaatgactg aacacgtctt gttctctg         58


<210>  26
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO017

<400>  26
ctaattacat gactcgaggt cgacggtatc gttaagcgtt taacaaattg aaaaatctg        59


<210>  27
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO018

<400>  27
gaaagcatag caatctaatc taagtttaaa acaatgagaa gatacatcag aggtggt          57


<210>  28
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO019

<400>  28
ctaattacat gactcgaggt cgacggtatc gttatgcagc gttcaacaaa ttgaaaa          57


<210>  29
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO020

<400>  29
gaaagcatag caatctaatc taagtttaaa acaatgaccg aacaagtctt attctcag         58


<210>  30
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO021

<400>  30
ctaattacat gactcgaggt cgacggtatc gttaagcgtt caacaaattg aaaaatct         58


<210>  31
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO024

<400>  31
gaaagcatag caatctaatc taagtttaaa acaatgaact tacaatttga agaaagacca       60


<210>  32
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO025

<400>  32
ctaattacat gactcgaggt cgacggtatc gttacaaatc agctaaaggg tgttcac          57


<210>  33
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO026

<400>  33
gaaagcatag caatctaatc taagtttaaa acaatgaact tacactttga agaattgac        59


<210>  34
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO027

<400>  34
ctaattacat gactcgaggt cgacggtatc gttagtagtc agacaaatct gctaaag          57


<210>  35
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO028

<400>  35
gaaagcatag caatctaatc taagtttaaa acaatgacaa tccactgtga agtattaac        59


<210>  36
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO029

<400>  36
ctaattacat gactcgaggt cgacggtatc gttaaccaac gtcagccaaa gggtg            55


<210>  37
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO030

<400>  37
gaaagcatag caatctaatc taagtttaaa acaatgaatg tcacctttga agaaagag         58


<210>  38
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer YO031

<400>  38
ctaattacat gactcgaggt cgacggtatc gttatgccaa atcagctaaa gggtg            55


<210>  39
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F1

<400>  39
atgacgcaat ttgcatttgt gttccc                                            26


<210>  40
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer F2

<400>  40
ttaaagctcg agcgccgct                                                    19


