                         SEQUENCE LISTING

<110>  Keramati, Malihe
       Ahangari Cohan, Reza
       Afshari, Elnaz
       Parsian, Parsa
 
<120>  TRUNCATED HYALURONAN SYNTHASE AND POLYNUCLEOTIDE ENCODING THE 
       SAME

<130>  0051-0007-01000

<150>  US 63212669
<151>  2021-06-20

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  417
<212>  PRT
<213>  Streptococcus dysgalactiae subspecies equisimilis


<220>
<221>  TRANSMEM
<222>  (7)..(29)
<223>  MD1 (i.e., membrane-associated domain 1)

<220>
<221>  TRANSMEM
<222>  (34)..(56)
<223>  MD2 (i.e., membrane-associated domain 2)

<220>
<221>  TRANSMEM
<222>  (320)..(342)
<223>  MD3 (i.e., membrane-associated domain 3)

<220>
<221>  TRANSMEM
<222>  (352)..(371)
<223>  MD4 (i.e., membrane-associated domain 4)

<220>
<221>  TRANSMEM
<222>  (378)..(395)
<223>  MD5 (i.e., membrane-associated domain 5)

<300>
<308>  GenBank/QSG30229.1
<309>  2021-03-06
<313>  (5)..(421)

<400>  1

Leu Lys Asn Leu Ile Thr Val Val Ala Phe Ser Ile Phe Trp Val Leu 
1               5                   10                  15      


Leu Ile Tyr Val Asn Val Tyr Leu Phe Gly Ala Lys Gly Ser Leu Ser 
            20                  25                  30          


Ile Tyr Gly Phe Leu Leu Ile Ala Tyr Leu Leu Val Lys Met Ser Leu 
        35                  40                  45              


Ser Phe Phe Tyr Lys Pro Phe Lys Gly Arg Ala Gly Gln Tyr Lys Val 
    50                  55                  60                  


Ala Ala Ile Ile Pro Ser Tyr Asn Glu Asp Ala Glu Ser Leu Leu Glu 
65                  70                  75                  80  


Thr Leu Lys Ser Val Gln Gln Gln Thr Tyr Pro Leu Ala Glu Ile Tyr 
                85                  90                  95      


Val Val Asp Asp Gly Ser Ala Asp Glu Thr Gly Ile Lys Arg Ile Glu 
            100                 105                 110         


Asp Tyr Val Arg Glu Thr Gly Asp Leu Ser Ser Asn Val Ile Val His 
        115                 120                 125             


Arg Ser Glu Lys Asn Gln Gly Lys Arg His Ala Gln Ala Trp Ala Phe 
    130                 135                 140                 


Glu Arg Ser Asp Ala Asp Val Phe Leu Thr Val Asp Ser Asp Thr Tyr 
145                 150                 155                 160 


Ile Tyr Pro Asp Ala Leu Glu Glu Leu Leu Lys Thr Phe Asn Asp Pro 
                165                 170                 175     


Thr Val Phe Ala Ala Thr Gly His Leu Asn Val Arg Asn Arg Gln Thr 
            180                 185                 190         


Asn Leu Leu Thr Arg Leu Thr Asp Ile Arg Tyr Asp Asn Ala Phe Gly 
        195                 200                 205             


Val Glu Arg Ala Ala Gln Ser Val Thr Gly Asn Ile Leu Val Cys Ser 
    210                 215                 220                 


Gly Pro Leu Ser Val Tyr Arg Arg Glu Val Val Val Pro Asn Ile Asp 
225                 230                 235                 240 


Arg Tyr Ile Asn Gln Thr Phe Leu Gly Ile Pro Val Ser Ile Gly Asp 
                245                 250                 255     


Asp Arg Cys Leu Thr Asn Tyr Ala Thr Asp Leu Gly Lys Thr Val Tyr 
            260                 265                 270         


Gln Ser Thr Ala Lys Cys Ile Thr Asp Val Pro Asp Lys Met Ser Thr 
        275                 280                 285             


Tyr Leu Lys Gln Gln Asn Arg Trp Asn Lys Ser Phe Phe Arg Glu Ser 
    290                 295                 300                 


Ile Ile Ser Val Lys Lys Ile Met Asn Asn Pro Phe Val Ala Leu Trp 
305                 310                 315                 320 


Thr Ile Leu Glu Val Ser Met Phe Met Met Leu Val Tyr Ser Val Val 
                325                 330                 335     


Asp Phe Phe Val Gly Asn Val Arg Glu Phe Asp Trp Leu Arg Val Leu 
            340                 345                 350         


Ala Phe Leu Val Ile Ile Phe Ile Val Ala Leu Cys Arg Asn Ile His 
        355                 360                 365             


Tyr Met Leu Lys His Pro Leu Ser Phe Leu Leu Ser Pro Phe Tyr Gly 
    370                 375                 380                 


Val Leu His Leu Phe Val Leu Gln Pro Leu Lys Leu Tyr Ser Leu Phe 
385                 390                 395                 400 


Thr Ile Arg Asn Ala Asp Trp Gly Thr Arg Lys Lys Leu Leu Leu Glu 
                405                 410                 415     


Arg 
   
<210>  2
<211>  159
<212>  PRT
<213>  Unknown

<220>
<223>  Truncated hyaluronan synthase (HAS), expressed in Escherichia 
       coli BL21

<400>  2

Pro Phe Lys Gly Arg Ala Gly Gln Tyr Lys Val Ala Ala Ile Ile Pro 
1               5                   10                  15      


Ser Tyr Asn Glu Asp Ala Glu Ser Leu Leu Glu Thr Leu Lys Ser Val 
            20                  25                  30          


Gln Gln Gln Thr Tyr Pro Leu Ala Glu Ile Tyr Val Val Asp Asp Gly 
        35                  40                  45              


Ser Ala Asp Glu Thr Gly Ile Lys Arg Ile Glu Asp Tyr Val Arg Glu 
    50                  55                  60                  


Thr Gly Asp Leu Ser Ser Asn Val Ile Val His Arg Ser Glu Lys Asn 
65                  70                  75                  80  


Gln Gly Lys Arg His Ala Gln Ala Trp Ala Phe Glu Arg Ser Asp Ala 
                85                  90                  95      


Asp Val Phe Leu Thr Val Asp Ser Asp Thr Tyr Ile Tyr Pro Asp Ala 
            100                 105                 110         


Leu Glu Glu Leu Leu Lys Thr Phe Asn Asp Pro Thr Val Phe Ala Ala 
        115                 120                 125             


Thr Gly His Leu Asn Val Arg Asn Arg Gln Thr Asn Leu Leu Thr Arg 
    130                 135                 140                 


Leu Thr Asp Ile Arg Tyr Asp Asn Ala Phe Gly Val Glu Arg Ala 
145                 150                 155                 


<210>  3
<211>  482
<212>  DNA
<213>  Unknown

<220>
<223>  Nucleic acid sequence of the truncated hyaluronan synthase (HAS) 
       gene that may encode the truncated HAS set forth in SEQ ID NO: 1

<400>  3
aagccattta agggaagggc tgggcaatat aaggttgcag ccattattcc ctcttataac       60

gaagatgctg agtcattgct agagacctta aaaagtgttc agcagcaaac ctatccccta      120

gcagaaattt atgttgttga cgatggaagt gctgatgaga caggtattaa gcgcattgaa      180

gactatgtgc gtgaaactgg tgacctatca agcaatgtca ttgttcaccg gtcagagaaa      240

aatcaaggaa agcgtcatgc acaggcctgg gcctttgaaa gatcagacgc tgatgtcttt      300

ttgaccgttg actcagatac ttatatctac cctgatgctt tagaggagtt gttaaaaacc      360

tttaatgacc caactgtttt tgctgcgacg ggtcatctta atgtcagaaa tagacaaacc      420

aatctcttaa cacgcttgac agatattcgc tatgataatg cttttggtgt tgaacgagct      480

gc                                                                     482


<210>  4
<211>  1261
<212>  DNA
<213>  Streptococcus dysgalactiae subspecies equisimilis

<300>
<308>  GenBank/MW285140.1
<309>  2021-03-06
<313>  (4)..(1264)

<400>  4
atgagaacat taaaaaacct cataactgtt gtggccttta gtattttttg ggtactgttg       60

atttacgtca atgtttatct ctttggtgct aaaggaagct tgtcaattta tggcttttta      120

ctgatagctt acctattagt caaaatgtcc ttatcctttt tttacaagcc atttaaggga      180

agggctgggc aatataaggt tgcagccatt attccctctt ataacgaaga tgctgagtca      240

ttgctagaga ccttaaaaag tgttcagcag caaacctatc ccctagcaga aatttatgtt      300

gttgacgatg gaagtgctga tgagacaggt attaagcgca ttgaagacta tgtgcgtgaa      360

actggtgacc tatcaagcaa tgtcattgtt caccggtcag agaaaaatca aggaaagcgt      420

catgcacagg cctgggcctt tgaaagatca gacgctgatg tctttttgac cgttgactca      480

gatacttata tctaccctga tgctttagag gagttgttaa aaacctttaa tgacccaact      540

gtttttgctg cgacgggtca tcttaatgtc agaaatagac aaaccaatct cttaacacgc      600

ttgacagata ttcgctatga taatgctttt ggtgttgaac gagctgccca atccgttaca      660

ggtaatattc tcgtttgctc aggcccgctt agcgtttaca gacgcgaggt ggttgttcct      720

aacatagata gatacatcaa ccagaccttc ctgggtattc ctgtaagtat cggtgatgac      780

aggtgcttga ccaactatgc aactgattta ggaaagactg tttatcaatc cactgctaaa      840

tgtattacag atgttcctga caagatgtct acttacttga agcagcaaaa ccgctggaac      900

aagtccttct ttagagagtc cattatttct gttaagaaaa tcatgaacaa tccttttgta      960

gccctatgga ccatacttga ggtgtctatg tttatgatgc ttgtttattc tgtggtggat     1020

ttctttgtag gcaatgtcag agaatttgat tggctcaggg ttttagcctt tctggtgatt     1080

atcttcattg ttgctctttg tcgtaatatt cactatatgc ttaagcaccc gctgtccttc     1140

ttgttatctc cgttttatgg ggtactgcat ttgtttgtcc tacagccctt gaaattgtat     1200

tctcttttta ctattagaaa tgctgactgg ggaacacgta aaaaattatt actcgagcgg     1260

g                                                                     1261


<210>  5
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying the full-length hyaluronan synthase
       (HAS) gene set forth in SEQ ID NO: 4, using the HAS nucleic acid 
       sequence of S. zooepidemicus (GenBank: AF023876.1) as template

<400>  5
ggaattccat atgagaacat taaaaaacct cataactg                               38


<210>  6
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying the full-length hyaluronan synthase
       (HAS) gene set forth in SEQ ID NO: 4, using the HAS nucleic acid 
       sequence of S. zooepidemicus (GenBank: AF023876.1) as template

<400>  6
cccgctcgag taataatttt ttacgtgttc c                                      31


<210>  7
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer for amplifying the truncated hyaluronan synthase 
       (HAS) gene set forth in SEQ ID NO: 3, using the full-length HAS 
       gene set forth in SEQ ID NO: 4 as template

<400>  7
taccatatga agccatttaa gggaagg                                           27


<210>  8
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer for amplifying the truncated hyaluronan synthase 
       (HAS) gene set forth in SEQ ID NO: 3, using the full-length HAS 
       gene set forth in SEQ ID NO: 4 as template

<400>  8
atactcgaga ggattgttca tgattttctt aacag                                  35


