               SEQUENCE LISTING

<110> BERYSOL GmbH

<120> Composite Biomarker for the Identification of Selenium Deficiency in a Bodily Fluid

<130> BY022429-P-WO

<150> DE102021209147.4
<151> 2021-08-19
<150> DE102021206749.2
<151> 2021-06-29

<160> 8

<170> BiSSAP 1.3.6

<210> 1
<211> 30
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer P1

<400> 1
atagatatca tgctgctgct gctgctgctg                                     30


<210> 2
<211> 32
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer P2

<400> 2
atagcggccg ccccgactct agagtaaccc gg                                   32


<210> 3
<211> 4677
<212> DNA
<213> Artificial Sequence


<220> 
<223> Expreesion vector containing cDNA of SEAP

<400> 3
ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttcg       60

aatcgcgaat tcgcccacca tgctgctgct gctgctgctg ctgggcctga ggctacagct      120

ctccctgggc atcatcccag ttgaggagga gaacccggac ttctggaacc gcgaggcagc      180

cgaggccctg ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat      240

catcttcctg ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg      300

gcagaagaag gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt      360

ggctctgtcc aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac      420

ggcctacctg tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg      480

ctttaaccag tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa      540

gaaagcaggg aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc      600

cggcacctac gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc      660

ggcccgccag gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga      720

cgtgatccta ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta      780

cccagatgac tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg      840

gctggcgaag cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc      900

cctggacccg tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga      960

gatccaccga gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg     1020

cctgctgagc aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca     1080

tggtcatcat gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc     1140

cattgagagg gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga     1200

ccactcccac gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct     1260

ggcccctggc aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc     1320

aggctatgtg ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc     1380

cgagtatcgg cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt     1440

ggcggtgttc gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt     1500

catagcgcac gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc     1560

gccccccgcc ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg     1620

ccgcttcgag cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg     1680

cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt     1740

ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag     1800

ggggaggtgt gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat     1860

aaggatccgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc     1920

gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg     1980

acaggtgccg gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc     2040

ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag     2100

gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa     2160

aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc     2220

gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc     2280

ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg     2340

cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt     2400

cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc     2460

gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc     2520

cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag     2580

agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg     2640

ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa     2700

ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag     2760

gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact     2820

cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa     2880

attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt     2940

accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag     3000

ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca     3060

gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc     3120

agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt     3180

ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg     3240

ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca     3300

gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg     3360

ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca     3420

tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg     3480

tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct     3540

cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca     3600

tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca     3660

gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg     3720

tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac     3780

ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt     3840

attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc     3900

cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg     3960

cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc     4020

ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa     4080

atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac     4140

ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt     4200

tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca     4260

accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt     4320

taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta     4380

caatttgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct     4440

cttcgctatt acgccagccc aagctaccat gataagtaag taatattaag gtacgggagg     4500

tacttggagc ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt     4560

gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact     4620

agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct atcgata        4677


<210> 4
<211> 36
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer P3

<400> 4
atagcggccg ctgagagcca ggaccaaagc tcctta                               36


<210> 5
<211> 33
<212> DNA
<213> Artificial Sequence


<220> 
<223> Primer P4

<400> 5
atagaattct tagtttgaag ggcattcgca ctt                                  33


<210> 6
<211> 1089
<212> DNA
<213> Homo sapiens


<220> 
<223> cDNA SELENOP

<400> 6
gagagccagg accaaagctc cttatgtaag caacccccag cctggagcat aagagatcaa      60

gatccaatgc taaactccaa tggttcagtg actgtggttg ctcttcttca agccagctgc     120

tacctgtgca tactgcaggc atctaaatta gaagacctgc gagtaaaact gaagaaagaa     180

ggatattcta atatttctta tattgttgtt aatcatcaag gaatctcttc tcgattaaaa     240

tacacacatc ttaagaataa ggtttcagag catattcctg tttatcaaca agaagaaaac     300

caaacagatg tctggactct tttaaatgga agcaaagatg acttcctcat atatgataga     360

tgtggccgtc ttgtatatca tcttggtttg cctttttcct tcctaacttt cccatatgta     420

gaagaagcca ttaagattgc ttactgtgaa aagaaatgtg gaaactgctc tctcacgact     480

ctcaaagatg aagacttttg taaacgtgta tctttggcta ctgtggataa aacagttgaa     540

actccatcgc ctcattacca tcatgagcat catcacaatc atggacatca gcaccttggc     600

agcagtgagc tttcagagaa tcagcaacca ggagcaccaa atgctcctac tcatcctgct     660

cctccaggcc ttcatcacca ccataagcac aagggtcagc ataggcaggg tcacccagag     720

aaccgagata tgccagcaag tgaagattta caagatttac aaaagaagct ctgtcgaaag     780

agatgtataa atcaattact ctgtaaattg cccacagatt cagagttggc tcctaggagc     840

tgctgctgcc attgtcgaca tctgatattt gaaaaaacag ggtctgcaat cacctgccag     900

tgtaaagaaa acctcccatc tttatgtagc tgccagggac ttcgggcaga ggagaacata     960

actgaatctt gtcagtgccg tttgcctcca gctgcctgcc aaataagtca gcagcttata    1020

cccacagaag ccagtgccag ttgccgctgc aagaatcagg caaaaaagtg cgaatgccct    1080

tcaaactaa                                                            1089


<210> 7
<211> 2631
<212> DNA
<213> Artificial Sequence


<220> 
<223> cDNA of fusion protein SEAP-SELENOP

<400> 7
atgctgctgc tgctgctgct gctgggcctg aggctacagc tctccctggg catcatccca      60

gttgaggagg agaacccgga cttctggaac cgcgaggcag ccgaggccct gggtgccgcc     120

aagaagctgc agcctgcaca gacagccgcc aagaacctca tcatcttcct gggcgatggg     180

atgggggtgt ctacggtgac agctgccagg atcctaaaag ggcagaagaa ggacaaactg     240

gggcctgaga tacccctggc catggaccgc ttcccatatg tggctctgtc caagacatac     300

aatgtagaca aacatgtgcc agacagtgga gccacagcca cggcctacct gtgcggggtc     360

aagggcaact tccagaccat tggcttgagt gcagccgccc gctttaacca gtgcaacacg     420

acacgcggca acgaggtcat ctccgtgatg aatcgggcca agaaagcagg gaagtcagtg     480

ggagtggtaa ccaccacacg agtgcagcac gcctcgccag ccggcaccta cgcccacacg     540

gtgaaccgca actggtactc ggacgccgac gtgcctgcct cggcccgcca ggaggggtgc     600

caggacatcg ctacgcagct catctccaac atggacattg acgtgatcct aggtggaggc     660

cgaaagtaca tgtttcgcat gggaacccca gaccctgagt acccagatga ctacagccaa     720

ggtgggacca ggctggacgg gaagaatctg gtgcaggaat ggctggcgaa gcgccagggt     780

gcccggtatg tgtggaaccg cactgagctc atgcaggctt ccctggaccc gtctgtgacc     840

catctcatgg gtctctttga gcctggagac atgaaatacg agatccaccg agactccaca     900

ctggacccct ccctgatgga gatgacagag gctgccctgc gcctgctgag caggaacccc     960

cgcggcttct tcctcttcgt ggagggtggt cgcatcgacc atggtcatca tgaaagcagg    1020

gcttaccggg cactgactga gacgatcatg ttcgacgacg ccattgagag ggcgggccag    1080

ctcaccagcg aggaggacac gctgagcctc gtcactgccg accactccca cgtcttctcc    1140

ttcggaggct accccctgcg agggagctcc atcttcgggc tggcccctgg caaggcccgg    1200

gacaggaagg cctacacggt cctcctatac ggaaacggtc caggctatgt gctcaaggac    1260

ggcgcccggc cggatgttac cgagagcgag agcgggagcc ccgagtatcg gcagcagtca    1320

gcagtgcccc tggacgaaga gacccacgca ggcgaggacg tggcggtgtt cgcgcgcggc    1380

ccgcaggcgc acctggttca cggcgtgcag gagcagacct tcatagcgca cgtcatggcc    1440

ttcgccgcct gcctggagcc ctacaccgcc tgcgacctgg cgccccccgc cggcaccacc    1500

gacgccgcgc acccgggtta ctctagagtc ggggcggccg ctgagagcca ggaccaaagc    1560

tccttatgta agcaaccccc agcctggagc ataagagatc aagatccaat gctaaactcc    1620

aatggttcag tgactgtggt tgctcttctt caagccagct gctacctgtg catactgcag    1680

gcatctaaat tagaagacct gcgagtaaaa ctgaagaaag aaggatattc taatatttct    1740

tatattgttg ttaatcatca aggaatctct tctcgattaa aatacacaca tcttaagaat    1800

aaggtttcag agcatattcc tgtttatcaa caagaagaaa accaaacaga tgtctggact    1860

cttttaaatg gaagcaaaga tgacttcctc atatatgata gatgtggccg tcttgtatat    1920

catcttggtt tgcctttttc cttcctaact ttcccatatg tagaagaagc cattaagatt    1980

gcttactgtg aaaagaaatg tggaaactgc tctctcacga ctctcaaaga tgaagacttt    2040

tgtaaacgtg tatctttggc tactgtggat aaaacagttg aaactccatc gcctcattac    2100

catcatgagc atcatcacaa tcatggacat cagcaccttg gcagcagtga gctttcagag    2160

aatcagcaac caggagcacc aaatgctcct actcatcctg ctcctccagg ccttcatcac    2220

caccataagc acaagggtca gcataggcag ggtcacccag agaaccgaga tatgccagca    2280

agtgaagatt tacaagattt acaaaagaag ctctgtcgaa agagatgtat aaatcaatta    2340

ctctgtaaat tgcccacaga ttcagagttg gctcctagga gctgctgctg ccattgtcga    2400

catctgatat ttgaaaaaac agggtctgca atcacctgcc agtgtaaaga aaacctccca    2460

tctttatgta gctgccaggg acttcgggca gaggagaaca taactgaatc ttgtcagtgc    2520

cgtttgcctc cagctgcctg ccaaataagt cagcagctta tacccacaga agccagtgcc    2580

agttgccgct gcaagaatca ggcaaaaaag tgcgaatgcc cttcaaacta a             2631


<210> 8
<211> 876
<212> PRT
<213> Artificial Sequence


<220> 
<223> Fusion Protein SEAP-SELENOP

<400> 8
Met Leu Leu Leu Leu Leu Leu Leu Gly Leu Arg Leu Gln Leu Ser Leu 
1               5                   10                  15      
Gly Ile Ile Pro Val Glu Glu Glu Asn Pro Asp Phe Trp Asn Arg Glu 
            20                  25                  30          
Ala Ala Glu Ala Leu Gly Ala Ala Lys Lys Leu Gln Pro Ala Gln Thr 
        35                  40                  45              
Ala Ala Lys Asn Leu Ile Ile Phe Leu Gly Asp Gly Met Gly Val Ser 
    50                  55                  60                  
Thr Val Thr Ala Ala Arg Ile Leu Lys Gly Gln Lys Lys Asp Lys Leu 
65                  70                  75                  80  
Gly Pro Glu Ile Pro Leu Ala Met Asp Arg Phe Pro Tyr Val Ala Leu 
                85                  90                  95      
Ser Lys Thr Tyr Asn Val Asp Lys His Val Pro Asp Ser Gly Ala Thr 
            100                 105                 110         
Ala Thr Ala Tyr Leu Cys Gly Val Lys Gly Asn Phe Gln Thr Ile Gly 
        115                 120                 125             
Leu Ser Ala Ala Ala Arg Phe Asn Gln Cys Asn Thr Thr Arg Gly Asn 
    130                 135                 140                 
Glu Val Ile Ser Val Met Asn Arg Ala Lys Lys Ala Gly Lys Ser Val 
145                 150                 155                 160 
Gly Val Val Thr Thr Thr Arg Val Gln His Ala Ser Pro Ala Gly Thr 
                165                 170                 175     
Tyr Ala His Thr Val Asn Arg Asn Trp Tyr Ser Asp Ala Asp Val Pro 
            180                 185                 190         
Ala Ser Ala Arg Gln Glu Gly Cys Gln Asp Ile Ala Thr Gln Leu Ile 
        195                 200                 205             
Ser Asn Met Asp Ile Asp Val Ile Leu Gly Gly Gly Arg Lys Tyr Met 
    210                 215                 220                 
Phe Arg Met Gly Thr Pro Asp Pro Glu Tyr Pro Asp Asp Tyr Ser Gln 
225                 230                 235                 240 
Gly Gly Thr Arg Leu Asp Gly Lys Asn Leu Val Gln Glu Trp Leu Ala 
                245                 250                 255     
Lys Arg Gln Gly Ala Arg Tyr Val Trp Asn Arg Thr Glu Leu Met Gln 
            260                 265                 270         
Ala Ser Leu Asp Pro Ser Val Thr His Leu Met Gly Leu Phe Glu Pro 
        275                 280                 285             
Gly Asp Met Lys Tyr Glu Ile His Arg Asp Ser Thr Leu Asp Pro Ser 
    290                 295                 300                 
Leu Met Glu Met Thr Glu Ala Ala Leu Arg Leu Leu Ser Arg Asn Pro 
305                 310                 315                 320 
Arg Gly Phe Phe Leu Phe Val Glu Gly Gly Arg Ile Asp His Gly His 
                325                 330                 335     
His Glu Ser Arg Ala Tyr Arg Ala Leu Thr Glu Thr Ile Met Phe Asp 
            340                 345                 350         
Asp Ala Ile Glu Arg Ala Gly Gln Leu Thr Ser Glu Glu Asp Thr Leu 
        355                 360                 365             
Ser Leu Val Thr Ala Asp His Ser His Val Phe Ser Phe Gly Gly Tyr 
    370                 375                 380                 
Pro Leu Arg Gly Ser Ser Ile Phe Gly Leu Ala Pro Gly Lys Ala Arg 
385                 390                 395                 400 
Asp Arg Lys Ala Tyr Thr Val Leu Leu Tyr Gly Asn Gly Pro Gly Tyr 
                405                 410                 415     
Val Leu Lys Asp Gly Ala Arg Pro Asp Val Thr Glu Ser Glu Ser Gly 
            420                 425                 430         
Ser Pro Glu Tyr Arg Gln Gln Ser Ala Val Pro Leu Asp Glu Glu Thr 
        435                 440                 445             
His Ala Gly Glu Asp Val Ala Val Phe Ala Arg Gly Pro Gln Ala His 
    450                 455                 460                 
Leu Val His Gly Val Gln Glu Gln Thr Phe Ile Ala His Val Met Ala 
465                 470                 475                 480 
Phe Ala Ala Cys Leu Glu Pro Tyr Thr Ala Cys Asp Leu Ala Pro Pro 
                485                 490                 495     
Ala Gly Thr Thr Asp Ala Ala His Pro Gly Tyr Ser Arg Val Gly Ala 
            500                 505                 510         
Ala Ala Glu Ser Gln Asp Gln Ser Ser Leu Cys Lys Gln Pro Pro Ala 
        515                 520                 525             
Trp Ser Ile Arg Asp Gln Asp Pro Met Leu Asn Ser Asn Gly Ser Val 
    530                 535                 540                 
Thr Val Val Ala Leu Leu Gln Ala Ser Cys Tyr Leu Cys Ile Leu Gln 
545                 550                 555                 560 
Ala Ser Lys Leu Glu Asp Leu Arg Val Lys Leu Lys Lys Glu Gly Tyr 
                565                 570                 575     
Ser Asn Ile Ser Tyr Ile Val Val Asn His Gln Gly Ile Ser Ser Arg 
            580                 585                 590         
Leu Lys Tyr Thr His Leu Lys Asn Lys Val Ser Glu His Ile Pro Val 
        595                 600                 605             
Tyr Gln Gln Glu Glu Asn Gln Thr Asp Val Trp Thr Leu Leu Asn Gly 
    610                 615                 620                 
Ser Lys Asp Asp Phe Leu Ile Tyr Asp Arg Cys Gly Arg Leu Val Tyr 
625                 630                 635                 640 
His Leu Gly Leu Pro Phe Ser Phe Leu Thr Phe Pro Tyr Val Glu Glu 
                645                 650                 655     
Ala Ile Lys Ile Ala Tyr Cys Glu Lys Lys Cys Gly Asn Cys Ser Leu 
            660                 665                 670         
Thr Thr Leu Lys Asp Glu Asp Phe Cys Lys Arg Val Ser Leu Ala Thr 
        675                 680                 685             
Val Asp Lys Thr Val Glu Thr Pro Ser Pro His Tyr His His Glu His 
    690                 695                 700                 
His His Asn His Gly His Gln His Leu Gly Ser Ser Glu Leu Ser Glu 
705                 710                 715                 720 
Asn Gln Gln Pro Gly Ala Pro Asn Ala Pro Thr His Pro Ala Pro Pro 
                725                 730                 735     
Gly Leu His His His His Lys His Lys Gly Gln His Arg Gln Gly His 
            740                 745                 750         
Pro Glu Asn Arg Asp Met Pro Ala Ser Glu Asp Leu Gln Asp Leu Gln 
        755                 760                 765             
Lys Lys Leu Cys Arg Lys Arg Cys Ile Asn Gln Leu Leu Cys Lys Leu 
    770                 775                 780                 
Pro Thr Asp Ser Glu Leu Ala Pro Arg Ser Cys Cys Cys His Cys Arg 
785                 790                 795                 800 
His Leu Ile Phe Glu Lys Thr Gly Ser Ala Ile Thr Cys Gln Cys Lys 
                805                 810                 815     
Glu Asn Leu Pro Ser Leu Cys Ser Cys Gln Gly Leu Arg Ala Glu Glu 
            820                 825                 830         
Asn Ile Thr Glu Ser Cys Gln Cys Arg Leu Pro Pro Ala Ala Cys Gln 
        835                 840                 845             
Ile Ser Gln Gln Leu Ile Pro Thr Glu Ala Ser Ala Ser Cys Arg Cys 
    850                 855                 860                 
Lys Asn Gln Ala Lys Lys Cys Glu Cys Pro Ser Asn 
865                 870                 875     

