﻿                         序列表

<110>  杭州师范大学

<120>  可卡因酯酶突变体及其应用

<160>  19

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  574
<212>  PRT
<213>  红球菌(Rhodococcus sp.)

<400>  1
Met Val Asp Gly Asn Tyr Ser Val Ala Ser Asn Val Met Val Pro Met 
1               5                   10                  15      
Arg Asp Gly Val Arg Leu Ala Val Asp Leu Tyr Arg Pro Asp Ala Asp 
            20                  25                  30          
Gly Pro Val Pro Val Leu Leu Val Arg Asn Pro Tyr Asp Lys Phe Asp 
        35                  40                  45              
Val Phe Ala Trp Ser Thr Gln Ser Thr Asn Trp Leu Glu Phe Val Arg 
    50                  55                  60                  
Asp Gly Tyr Ala Val Val Ile Gln Asp Thr Arg Gly Leu Phe Ala Ser 
65                  70                  75                  80  
Glu Gly Glu Phe Val Pro His Val Asp Asp Glu Ala Asp Ala Glu Asp 
                85                  90                  95      
Thr Leu Ser Trp Ile Leu Glu Gln Ala Trp Cys Asp Gly Asn Val Gly 
            100                 105                 110         
Met Phe Gly Val Ser Tyr Leu Gly Val Thr Gln Trp Gln Ala Ala Val 
        115                 120                 125             
Ser Gly Val Gly Gly Leu Lys Ala Ile Ala Pro Ser Met Ala Ser Ala 
    130                 135                 140                 
Asp Leu Tyr Arg Ala Pro Trp Tyr Gly Pro Gly Gly Ala Leu Ser Val 
145                 150                 155                 160 
Glu Ala Leu Leu Gly Trp Ser Ala Leu Ile Gly Arg Gln Leu Ile Thr 
                165                 170                 175     
Ser Arg Ser Asp Ala Arg Pro Glu Asp Ala Ala Asp Phe Val Gln Leu 
            180                 185                 190         
Ala Ala Ile Cys Asn Asp Val Ala Gly Ala Ala Ser Val Thr Pro Leu 
        195                 200                 205             
Ala Glu Gln Pro Leu Leu Gly Arg Leu Ile Pro Trp Val Ile Asp Gln 
    210                 215                 220                 
Val Val Asp His Pro Asp Asn Asp Glu Ser Trp Gln Ser Ile Ser Leu 
225                 230                 235                 240 
Phe Glu Arg Leu Gly Gly Leu Ala Thr Pro Ala Leu Ile Thr Ala Gly 
                245                 250                 255     
Trp Tyr Asp Gly Phe Val Gly Glu Ser Leu Arg Thr Phe Val Ala Val 
            260                 265                 270         
Lys Asp Asn Ala Asp Ala Arg Leu Val Val Gly Pro Trp Ser His Ser 
        275                 280                 285             
Asn Leu Thr Gly Arg Asn Ala Asp Arg Lys Phe Gly Cys Ala Ala Thr 
    290                 295                 300                 
Tyr Pro Ile Gln Glu Ala Thr Thr Met His Lys Ala Phe Phe Asp Arg 
305                 310                 315                 320 
His Leu Arg Gly Glu Thr Asp Ala Leu Ala Gly Val Pro Lys Val Arg 
                325                 330                 335     
Leu Phe Val Met Gly Ile Asp Glu Trp Arg Asp Glu Thr Asp Trp Pro 
            340                 345                 350         
Leu Pro Asp Thr Ala Tyr Thr Pro Phe Tyr Leu Gly Gly Ser Gly Ala 
        355                 360                 365             
Ala Asn Thr Ser Thr Gly Gly Gly Thr Leu Ser Thr Ser Ile Ser Gly 
    370                 375                 380                 
Thr Glu Ser Ala Asp Thr Tyr Leu Tyr Asp Pro Ala Asp Pro Val Pro 
385                 390                 395                 400 
Ser Leu Gly Gly Thr Leu Leu Phe His Asn Gly Asp Asn Gly Pro Ala 
                405                 410                 415     
Asp Gln Arg Pro Ile His Asp Arg Asp Asp Val Leu Cys Tyr Ser Thr 
            420                 425                 430         
Glu Val Leu Thr Asp Pro Val Glu Val Thr Gly Thr Val Ser Ala Arg 
        435                 440                 445             
Leu Phe Val Ser Ser Ser Ala Val Asp Thr Asp Phe Thr Ala Lys Leu 
    450                 455                 460                 
Val Asp Val Phe Pro Asp Gly Arg Ala Ile Ala Leu Cys Asp Gly Ile 
465                 470                 475                 480 
Val Arg Met Arg Tyr Arg Glu Thr Leu Val Asn Pro Thr Leu Ile Glu 
                485                 490                 495     
Ala Gly Glu Ile Tyr Glu Val Ala Ile Asp Met Leu Ala Thr Ser Asn 
            500                 505                 510         
Val Phe Leu Pro Gly His Arg Ile Met Val Gln Val Ser Ser Ser Asn 
        515                 520                 525             
Phe Pro Lys Tyr Asp Arg Asn Ser Asn Thr Gly Gly Val Ile Ala Arg 
    530                 535                 540                 
Glu Gln Leu Glu Glu Met Cys Thr Ala Val Asn Arg Ile His Arg Gly 
545                 550                 555                 560 
Pro Glu His Pro Ser His Ile Val Leu Pro Ile Ile Lys Arg 
                565                 570                 

<210>  2
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
ctctcatagg tcgccagctc atcacgtc                                           28

<210>  3
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
gacgtgatga gctggcgacc tatgagag                                           28

<210>  4
<211>  25
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
ctcgcagcaa tttgcaatga cgtcg                                              25

<210>  5
<211>  25
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
cgacgtcatt gcaaattgct gcgag                                              25

<210>  6
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  6
ggaagttcgg ctgcgccgcg acctac                                             26

<210>  7
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  7
gtaggtcgcg gcgcagccga acttcc                                             26

<210>  8
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  8
atgtgggcat gttcggcttt tcgtacttgg gt                                      32

<210>  9
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  9
acccaagtac gaaaagccga acatgcccac at                                      32

<210>  10
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
gtgttcttgt ggtcgacgca gt                                                 22

<210>  11
<211>  22
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
actgcgtcga ccacaagaac ac                                                 22

<210>  12
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
ttcgacgtgt tctactggtc gacgcagtcg                                         30

<210>  13
<211>  30
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
cgactgcgtc gaccagtaga acacgtcgaa                                         30

<210>  14
<211>  23
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
gacgtgttcg tttggtcgac gca                                                23

<210>  15
<211>  23
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  15
tgcgtcgacc aaacgaacac gtc                                                23

<210>  16
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  16
ccatacgaca agttcgacgt gttcttctgg tc                                      32

<210>  17
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
gaccagaaga acacgtcgaa cttgtcgtat gg                                      32

<210>  18
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  18
ccatacgaca agttcgacgt gttctggtgg tc                                      32

<210>  19
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  19
gaccaccaga acacgtcgaa cttgtcgtat gg                                      32