                         SEQUENCE LISTING

<110>  ɶ̼Ƽ޹˾
 
<120>  ׵׵ͻ塢׿׼Ӧ

<130>  PCT21093CN

<160>  28    

<170>  PatentIn version 3.5

<210>  1
<211>  312
<212>  PRT
<213>  Thermodesulfovibrio

<400>  1

Met Phe Asn Phe Ile Lys Phe Lys Phe Ile Leu Ile Leu Phe Leu Pro 
1               5                   10                  15      


Pro Ile Leu Phe Gly Cys Met Ala Met Thr Pro Asn Ile Ala Ser Ile 
            20                  25                  30          


Arg Ala Thr Ser Gly Pro Glu Tyr Ile Thr Ser Ile His Arg Asp Leu 
        35                  40                  45              


Val Ser Leu Pro Lys Pro Glu Asn Pro Ile Pro Val Ala Val Tyr Lys 
    50                  55                  60                  


Phe Arg Asp Gln Thr Gly Gln Tyr Lys Ala Pro Gly Asn Ala Thr Asn 
65                  70                  75                  80  


Phe Ser Thr Ala Val Thr Gln Gly Ala Thr Ser Ile Leu Ile Lys Ala 
                85                  90                  95      


Leu Glu Asp Ser Gly Trp Phe Leu Pro Val Glu Arg Glu Gly Leu Ala 
            100                 105                 110         


Asn Leu Leu Gln Glu Arg Lys Ile Val Leu Gln Met Arg Glu Leu Tyr 
        115                 120                 125             


Leu Thr Glu Glu Gln Lys Lys Gln Phe Glu Pro Leu Pro Pro Leu Leu 
    130                 135                 140                 


Tyr Ala Gly Ile Ile Phe Glu Gly Gly Ile Ile Gly Tyr Asp Ser Asn 
145                 150                 155                 160 


Val Thr Thr Gly Gly Ile Gly Ala Lys Tyr Phe Gly Ala Gly Gly Ser 
                165                 170                 175     


Ala Glu Tyr Arg Val Asp Lys Val Ser Ile Tyr Leu Arg Ala Val Ser 
            180                 185                 190         


Val Lys Asn Gly Ala Val Leu Lys Thr Val Gln Thr Ser Lys Thr Val 
        195                 200                 205             


Leu Ser Gln Met Leu Ser Leu Gly Ile Phe Arg Phe Val Arg Leu Asn 
    210                 215                 220                 


Arg Leu Leu Glu Ala Glu Ala Gly Ile Ala Ala Asn Glu Pro Val Glu 
225                 230                 235                 240 


Met Ala Val Gln Glu Ala Ile Glu Lys Ala Val Tyr Asp Ile Ile Ile 
                245                 250                 255     


Glu Gly Val Lys Thr Gly Ile Trp Lys Pro Lys Asp Thr Lys Glu Phe 
            260                 265                 270         


Glu Lys Trp Leu Ser Lys Tyr Glu Asn Glu Leu Lys Glu Lys Thr Phe 
        275                 280                 285             


Ala Ser Glu Ser Ile Ser Lys Leu Ser Lys Gln Lys Glu Ser Lys Asp 
    290                 295                 300                 


Ile Val Lys Glu Leu Phe Lys Tyr 
305                 310         


<210>  2
<211>  939
<212>  DNA
<213>  Thermodesulfovibrio

<400>  2
atgttcaact tcatcaagtt caagttcatc ctgatcctgt tcctgccgcc gatcctgttt       60

ggttgcatgg cgatgacccc gaacattgcg agcatccgtg cgaccagcgg tccggagtac      120

attaccagca tccaccgtga cctggtgagc ctgccgaaac cggaaaatcc gatcccggtg      180

gcggtttaca aattccgtga tcagaccggt caatataagg cgccgggcaa cgcgaccaat      240

tttagcaccg cggttaccca gggtgcgacc agcattctga tcaaagcgct ggaggacagc      300

ggttggttcc tgccggtgga gcgtgaaggc ctggcgaacc tgctgcagga gcgcaagatc      360

gttctgcaaa tgcgtgaact gtacctgacc gaggaacaga agaaacaatt tgagccgctg      420

ccgccgctgc tgtatgcggg tatcattttt gaaggtggca tcattggcta tgacagcaac      480

gtgaccaccg gtggcattgg tgcgaaatat tttggtgcgg gtggcagcgc ggagtaccgt      540

gtggataaag ttagcatcta tctgcgtgct gtgagcgtta agaatggtgc ggtgctgaaa      600

accgttcaga ccagcaagac cgtgctgagc caaatgctga gcctgggtat tttccgtttt      660

gttcgcctga accgtctgct ggaggcggaa gcgggtattg cggcgaatga gccggtggaa      720

atggcggttc aggaagcgat cgaaaaagcg gtgtacgaca tcattatcga aggtgttaag      780

accggcatct ggaagccgaa agataccaag gagttcgaaa aatggctgag caagtatgag      840

aacgaactga aggagaaaac ctttgcgagc gaaagcatta gcaagctgag caagcaaaaa      900

gagagcaaag atatcgttaa ggaactgttc aaatattaa                             939


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  3
tttttttttt                                                              10


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  4
aatgtacttc gttcagttac gtattgct                                          28


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  5
gcaatacgta actgaacgaa gttcactatc gcattctcat ga                          42


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  6
tcatgagaat gcgatagtga                                                   20


<210>  7
<211>  89
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  7
aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aagcaatacg taactgaacg       60

aagtacatta aaaaaaaaaa aaaaaaaaa                                         89


<210>  8
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  8
atcctttttt tttttttttt tttt                                              24


<210>  9
<211>  48
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  9
aatgtacttc gttcagttac gtattgcttt tttttttttt tttttttt                    48


<210>  10
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-SE1

<400>  10
tttttttttt tttttttttt                                                   20


<210>  11
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  11
tttttttttt                                                              10


<210>  12
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  12
aatgtacttc gttcagttac gtattgct                                          28


<210>  13
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  13
gcaatacgta actgaacgaa gttcactatc gcattctcat ga                          42


<210>  14
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  14
tcatgagaat gcgatagtga                                                   20


<210>  15
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  15
aaaaaaaaaa aaaaaaaaaa aaaaaaaa                                          28


<210>  16
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  16
aaaaaaaaaa aa                                                           12


<210>  17
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  17
aaaaaaaaaa aaaatctctg aatctctgaa tctctgaatc tctaaaaaaa aaaaagaaaa       60

aaaaaaaaca aaaaaaaaaa ataaaaaaaa aaaaagcaat acgtaactga acgaagtaca      120

ttaaaaaaaa aa                                                          132


<210>  18
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  18
atcctttttt ttttaatgta cttcgttcag ttacgtattg ct                          42


<210>  19
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  19
tttttttttt ttattttttt tttttgtttt ttttttttct tttttttttt tagagattca       60

gagattcaga gattcagaga tttttttttt tttt                                   94


<210>  20
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  20
tttttttttt tt                                                           12


<210>  21
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  21
tttttttttt tttttttttt tttttttt                                          28


<210>  22
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  22

Met Ile Thr Ile Asp Gln Leu Thr Glu Gly Gln Phe Asp Ser Leu Gln 
1               5                   10                  15      


Arg Ala Lys Val Leu Ile Gln Glu Ala Thr Lys Asn Asp Gly Asn Trp 
            20                  25                  30          


Asn His Arg Thr Lys His Leu Thr Ile Asn Gly Pro Ala Gly Thr Gly 
        35                  40                  45              


Lys Thr Thr Met Met Lys Phe Leu Val Ser Trp Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Thr Gly Val Ala Leu Ala Ala Pro Thr His Ala Ala Lys Lys Val 
65                  70                  75                  80  


Leu Ala Asn Ala Val Gly Glu Glu Val Ser Thr Ile His Ser Ile Leu 
                85                  90                  95      


Lys Ile Asn Pro Thr Thr Tyr Glu Cys Lys Gln Phe Phe Glu Gln Ser 
            100                 105                 110         


Ala Pro Pro Asp Leu Ser Lys Ile Arg Ile Leu Ile Cys Glu Glu Cys 
        115                 120                 125             


Ser Phe Tyr Asp Ile Lys Leu Phe Glu Ile Leu Met Asn Ser Ile Gln 
    130                 135                 140                 


Pro Trp Thr Ile Ile Ile Gly Ile Gly Asp Arg Ala Gln Leu Arg Pro 
145                 150                 155                 160 


Ala Asp Asp Lys Gly Ile Ser Arg Phe Phe Thr Asp Gln Arg Phe Glu 
                165                 170                 175     


Gln Thr Tyr Leu Thr Glu Ile Lys Arg Ser Asn Met Pro Ile Ile Glu 
            180                 185                 190         


Val Ala Thr Glu Ile Arg Asn Gly Gly Trp Ile Arg Glu Asn Ile Ile 
        195                 200                 205             


Asp Asp Leu Gly Val Lys Gln Asp Lys Ser Val Ser Glu Phe Met Thr 
    210                 215                 220                 


Asn Tyr Phe Lys Val Val Lys Ser Ile Asp Asp Leu Tyr Glu Thr Arg 
225                 230                 235                 240 


Met Tyr Ala Tyr Thr Asn Asn Ser Val Asp Thr Leu Asn Lys Ile Ile 
                245                 250                 255     


Arg Lys Lys Leu Tyr Glu Thr Glu Gln Asp Phe Ile Val Gly Glu Pro 
            260                 265                 270         


Ile Val Met Gln Glu Pro Leu Ile Arg Asp Ile Asn Tyr Glu Gly Lys 
        275                 280                 285             


Arg Phe Gln Glu Ile Val Phe Asn Asn Gly Glu Tyr Leu Glu Val Ser 
    290                 295                 300                 


Glu Ile Lys Pro Met Glu Ser Val Leu Lys Cys Arg Asn Ile Asp Tyr 
305                 310                 315                 320 


Gln Leu Val Leu His Tyr Tyr Gln Leu Lys Val Lys Ser Ile Asp Thr 
                325                 330                 335     


Gly Glu Ser Gly Leu Ile Asn Thr Ile Ser Asp Lys Asn Glu Leu Asn 
            340                 345                 350         


Lys Phe Tyr Met Phe Leu Gly Lys Val Cys Gln Asp Tyr Lys Ser Gly 
        355                 360                 365             


Thr Ile Lys Ala Phe Trp Asp Asp Phe Trp Lys Ile Lys Asn Asn Tyr 
    370                 375                 380                 


His Arg Val Lys Pro Leu Pro Val Ser Thr Ile His Lys Gly Gln Gly 
385                 390                 395                 400 


Ser Thr Val Asp Asn Ser Phe Leu Tyr Thr Pro Cys Ile Thr Lys Tyr 
                405                 410                 415     


Ala Glu Pro Asp Leu Ala Ser Gln Leu Leu Tyr Val Gly Val Thr Arg 
            420                 425                 430         


Ala Arg His Asn Val Asn Phe Val Gly 
        435                 440     


<210>  23
<211>  1326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  23
atgatcacca tcgaccagct gaccgaaggt cagttcgact ctctgcagcg tgctaaagtt       60

ctgatccagg aagctaccaa aaacgacggt aactggaacc accgtaccaa acacctgacc      120

atcaacggtc cggctggtac cggtaaaacc accatgatga aattcctggt ttcttggctg      180

cgtgacgaag gtatcaccgg tgttgctctg gctgctccga cccacgctgc taaaaaagtt      240

ctggctaacg ctgttggtga agaagtttct accatccact ctatcctgaa aatcaacccg      300

accacctacg aatgcaaaca gttcttcgaa cagtctgctc cgccggacct gtctaaaatc      360

cgtatcctga tctgcgaaga atgctctttc tacgacatca aactgttcga aatcctgatg      420

aactctatcc agccgtggac catcatcatc ggtatcggtg accgtgctca gctgcgtccg      480

gctgacgaca aaggtatctc tcgtttcttc accgaccagc gtttcgaaca gacctacctg      540

accgaaatca aacgttctaa catgccgatc atcgaagttg ctaccgaaat ccgtaacggt      600

ggttggattc gtgaaaacat catcgacgac ctgggtgtta aacaggacaa atctgtttct      660

gaatttatga ccaactactt caaagttgtt aaatctatcg acgacctgta cgaaacccgt      720

atgtacgctt acaccaacaa ctctgttgac accctgaaca aaatcatccg taaaaaactg      780

tacgaaaccg aacaggactt catcgttggt gaaccgatcg ttatgcagga accgctgatc      840

cgtgacatca actacgaagg taaacgtttc caggaaatcg ttttcaacaa cggtgaatac      900

ctggaagttt ctgaaatcaa accgatggaa tctgttctga aatgccgtaa catcgactac      960

cagctggttc tgcactacta ccagctgaaa gttaaatcta tcgacaccgg tgaatctggt     1020

ctgatcaaca ccatctctga caaaaacgaa ctgaacaaat tctacatgtt cctgggtaaa     1080

gtttgccagg actacaaatc tggtaccatc aaagcgttct gggacgactt ctggaaaatc     1140

aaaaacaact accaccgtgt taaaccgctg ccggtttcta ccatccacaa aggtcagggt     1200

tctaccgttg acaactcttt cctgtacacc ccgtgcatca ccaaatacgc tgaaccggac     1260

ctggcttctc agctgctgta cgttggtgtt acccgtgctc gtcacaacgt taacttcgtt     1320

ggttaa                                                                1326


<210>  24
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 1

<400>  24

Met Phe Asn Phe Ile Lys Phe Lys Phe Ile Leu Ile Leu Phe Leu Pro 
1               5                   10                  15      


Pro Ile Leu Phe Gly Cys Met Ala Met Thr Pro Asn Ile Ala Ser Ile 
            20                  25                  30          


Arg Ala Thr Ser Gly Pro Glu Tyr Ile Thr Ser Ile His Arg Asp Leu 
        35                  40                  45              


Val Ser Leu Pro Lys Pro Glu Asn Pro Ile Pro Val Ala Val Tyr Lys 
    50                  55                  60                  


Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Tyr Pro Ala Asn Ala Ser 
65                  70                  75                  80  


Thr Ala Val Thr Gln Gly Ala Thr Ser Ile Leu Ile Lys Ala Leu Glu 
                85                  90                  95      


Asp Ser Gly Trp Phe Leu Pro Val Glu Arg Glu Gly Leu Ala Asn Leu 
            100                 105                 110         


Leu Gln Glu Arg Lys Ile Val Leu Gln Met Arg Glu Leu Tyr Leu Thr 
        115                 120                 125             


Glu Glu Gln Lys Lys Gln Phe Glu Pro Leu Pro Pro Leu Leu Tyr Ala 
    130                 135                 140                 


Gly Ile Ile Phe Glu Gly Gly Ile Ile Gly Tyr Asp Ser Asn Val Thr 
145                 150                 155                 160 


Thr Gly Gly Ile Gly Ala Lys Tyr Phe Gly Ala Gly Gly Ser Ala Glu 
                165                 170                 175     


Tyr Arg Val Asp Lys Val Ser Ile Tyr Leu Arg Ala Val Ser Val Lys 
            180                 185                 190         


Asn Gly Ala Val Leu Lys Thr Val Gln Thr Ser Lys Thr Val Leu Ser 
        195                 200                 205             


Gln Met Leu Ser Leu Gly Ile Phe Arg Phe Val Arg Leu Asn Arg Leu 
    210                 215                 220                 


Leu Glu Ala Glu Ala Gly Ile Ala Ala Asn Glu Pro Val Glu Met Ala 
225                 230                 235                 240 


Val Gln Glu Ala Ile Glu Lys Ala Val Tyr Asp Ile Ile Ile Glu Gly 
                245                 250                 255     


Val Lys Thr Gly Ile Trp Lys Pro Lys Asp Thr Lys Glu Phe Glu Lys 
            260                 265                 270         


Trp Leu Ser Lys Tyr Glu Asn Glu Leu Lys Glu Lys Thr Phe Ala Ser 
        275                 280                 285             


Glu Ser Ile Ser Lys Leu Ser Lys Gln Lys Glu Ser Lys Asp Ile Val 
    290                 295                 300                 


Lys Glu Leu Phe Lys Tyr 
305                 310 


<210>  25
<211>  933
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  mutant 1

<400>  25
atgtttaatt ttattaagtt taaattcata cttatcttgt tcctgccgcc tattctcttt       60

ggctgcatgg caatgacccc gaatattgcg tccattcgtg ctactagtgg tccggagtac      120

attacctcga ttcatcgtga cctggttagc ctgccgaaac cggaaaaccc gattccggta      180

gccgtttata aattccgcga ccagaccggt cagtataaac cgtacccggc taatgcttcg      240

accgcagtga cccagggtgc gacctctatc ctgatcaaag cactagaaga tagcggttgg      300

tttctcccag tggaacgcga gggcctggcg aatctgttgc aagagcgcaa aatcgtgctg      360

cagatgcgtg aactgtactt gaccgaagag cagaagaaac aatttgagcc gctcccaccg      420

ttgttgtacg caggcatcat cttcgaaggt ggcattatcg gctatgatag caacgtgacc      480

actggtggta tcggtgctaa gtattttggc gcgggtggta gcgcggaata ccgtgttgat      540

aaagtttcca tatatctgag agcggtgagc gttaagaacg gtgcggttct taagaccgtt      600

caaacctcga agacggtcct gtcccagatg ctgagcctgg gcatcttccg ctttgttcgt      660

ctgaaccgtc tgttagaggc cgaagctggc atcgctgcca atgaaccggt cgaaatggcg      720

gtgcaagagg cgattgaaaa agcggtctac gacattatta tcgagggcgt taagacgggt      780

atttggaaac cgaaagatac caaagagttc gaaaaatggc tgagcaagta cgagaacgag      840

ctgaaagaga agacgtttgc gtccgaatct atcagcaagc tgtcaaagca gaaggagagc      900

aaggacatcg tgaaggagct gttcaaatac tga                                   933


<210>  26
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 2

<400>  26

Met Phe Asn Phe Ile Lys Phe Lys Phe Ile Leu Ile Leu Phe Leu Pro 
1               5                   10                  15      


Pro Ile Leu Phe Gly Cys Met Ala Met Thr Pro Asn Ile Ala Ser Ile 
            20                  25                  30          


Arg Ala Thr Ser Gly Pro Glu Tyr Ile Thr Ser Ile His Arg Asp Leu 
        35                  40                  45              


Val Ser Leu Pro Lys Pro Glu Asn Pro Ile Pro Val Ala Val Tyr Lys 
    50                  55                  60                  


Phe Arg Asp Gln Thr Gly Gln Tyr Lys Ala His Pro Ala Ala Thr Ser 
65                  70                  75                  80  


Pro Ala Val Thr Gln Gly Ala Thr Ser Ile Leu Ile Lys Ala Leu Glu 
                85                  90                  95      


Asp Ser Gly Trp Phe Leu Pro Val Glu Arg Glu Gly Leu Ala Asn Leu 
            100                 105                 110         


Leu Gln Glu Arg Lys Ile Val Leu Gln Met Arg Glu Leu Tyr Leu Thr 
        115                 120                 125             


Glu Glu Gln Lys Lys Gln Phe Glu Pro Leu Pro Pro Leu Leu Tyr Ala 
    130                 135                 140                 


Gly Ile Ile Phe Glu Gly Gly Ile Ile Gly Tyr Asp Ser Asn Val Thr 
145                 150                 155                 160 


Thr Gly Gly Ile Gly Ala Lys Tyr Phe Gly Ala Gly Gly Ser Ala Glu 
                165                 170                 175     


Tyr Arg Val Asp Lys Val Ser Ile Tyr Leu Arg Ala Val Ser Val Lys 
            180                 185                 190         


Asn Gly Ala Val Leu Lys Thr Val Gln Thr Ser Lys Thr Val Leu Ser 
        195                 200                 205             


Gln Met Leu Ser Leu Gly Ile Phe Arg Phe Val Arg Leu Asn Arg Leu 
    210                 215                 220                 


Leu Glu Ala Glu Ala Gly Ile Ala Ala Asn Glu Pro Val Glu Met Ala 
225                 230                 235                 240 


Val Gln Glu Ala Ile Glu Lys Ala Val Tyr Asp Ile Ile Ile Glu Gly 
                245                 250                 255     


Val Lys Thr Gly Ile Trp Lys Pro Lys Asp Thr Lys Glu Phe Glu Lys 
            260                 265                 270         


Trp Leu Ser Lys Tyr Glu Asn Glu Leu Lys Glu Lys Thr Phe Ala Ser 
        275                 280                 285             


Glu Ser Ile Ser Lys Leu Ser Lys Gln Lys Glu Ser Lys Asp Ile Val 
    290                 295                 300                 


Lys Glu Leu Phe Lys Tyr 
305                 310 


<210>  27
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 3

<400>  27

Met Phe Asn Phe Ile Lys Phe Lys Phe Ile Leu Ile Leu Phe Leu Pro 
1               5                   10                  15      


Pro Ile Leu Phe Gly Cys Met Ala Met Thr Pro Asn Ile Ala Ser Ile 
            20                  25                  30          


Arg Ala Thr Ser Gly Pro Glu Tyr Ile Thr Ser Ile His Arg Asp Leu 
        35                  40                  45              


Val Ser Leu Pro Lys Pro Glu Asn Pro Ile Pro Val Ala Val Tyr Lys 
    50                  55                  60                  


Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Ala Ala Ser Ser Leu Ser 
65                  70                  75                  80  


Pro Ala Val Thr Gln Gly Ala Thr Ser Ile Leu Ile Lys Ala Leu Glu 
                85                  90                  95      


Asp Ser Gly Trp Phe Leu Pro Val Glu Arg Glu Gly Leu Ala Asn Leu 
            100                 105                 110         


Leu Gln Glu Arg Lys Ile Val Leu Gln Met Arg Glu Leu Tyr Leu Thr 
        115                 120                 125             


Glu Glu Gln Lys Lys Gln Phe Glu Pro Leu Pro Pro Leu Leu Tyr Ala 
    130                 135                 140                 


Gly Ile Ile Phe Glu Gly Gly Ile Ile Gly Tyr Asp Ser Asn Val Thr 
145                 150                 155                 160 


Thr Gly Gly Ile Gly Ala Lys Tyr Phe Gly Ala Gly Gly Ser Ala Glu 
                165                 170                 175     


Tyr Arg Val Asp Lys Val Ser Ile Tyr Leu Arg Ala Val Ser Val Lys 
            180                 185                 190         


Asn Gly Ala Val Leu Lys Thr Val Gln Thr Ser Lys Thr Val Leu Ser 
        195                 200                 205             


Gln Met Leu Ser Leu Gly Ile Phe Arg Phe Val Arg Leu Asn Arg Leu 
    210                 215                 220                 


Leu Glu Ala Glu Ala Gly Ile Ala Ala Asn Glu Pro Val Glu Met Ala 
225                 230                 235                 240 


Val Gln Glu Ala Ile Glu Lys Ala Val Tyr Asp Ile Ile Ile Glu Gly 
                245                 250                 255     


Val Lys Thr Gly Ile Trp Lys Pro Lys Asp Thr Lys Glu Phe Glu Lys 
            260                 265                 270         


Trp Leu Ser Lys Tyr Glu Asn Glu Leu Lys Glu Lys Thr Phe Ala Ser 
        275                 280                 285             


Glu Ser Ile Ser Lys Leu Ser Lys Gln Lys Glu Ser Lys Asp Ile Val 
    290                 295                 300                 


Lys Glu Leu Phe Lys Tyr 
305                 310 


<210>  28
<211>  310
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 4

<400>  28

Met Phe Asn Phe Ile Lys Phe Lys Phe Ile Leu Ile Leu Phe Leu Pro 
1               5                   10                  15      


Pro Ile Leu Phe Gly Cys Met Ala Met Thr Pro Asn Ile Ala Ser Ile 
            20                  25                  30          


Arg Ala Thr Ser Gly Pro Glu Tyr Ile Thr Ser Ile His Arg Asp Leu 
        35                  40                  45              


Val Ser Leu Pro Lys Pro Glu Asn Pro Ile Pro Val Ala Val Tyr Lys 
    50                  55                  60                  


Phe Arg Asp Gln Thr Gly Gln Tyr Lys Pro Tyr Pro Ala Asn Ala Ser 
65                  70                  75                  80  


Pro Ala Val Thr Gln Gly Ala Thr Ser Ile Leu Ile Lys Ala Leu Glu 
                85                  90                  95      


Asp Ser Gly Trp Phe Leu Pro Val Glu Arg Glu Gly Leu Ala Asn Leu 
            100                 105                 110         


Leu Gln Glu Arg Lys Ile Val Leu Gln Met Arg Glu Leu Tyr Leu Thr 
        115                 120                 125             


Glu Glu Gln Lys Lys Gln Phe Glu Pro Leu Pro Pro Leu Leu Tyr Ala 
    130                 135                 140                 


Gly Ile Ile Phe Glu Gly Gly Ile Ile Gly Tyr Asp Ser Asn Val Thr 
145                 150                 155                 160 


Thr Gly Gly Ile Gly Ala Lys Tyr Phe Gly Ala Gly Gly Ser Ala Glu 
                165                 170                 175     


Tyr Arg Val Asp Lys Val Ser Ile Tyr Leu Arg Ala Val Ser Val Lys 
            180                 185                 190         


Asn Gly Ala Val Leu Lys Thr Val Gln Thr Ser Lys Thr Val Leu Ser 
        195                 200                 205             


Gln Met Leu Ser Leu Gly Ile Phe Arg Phe Val Arg Leu Asn Arg Leu 
    210                 215                 220                 


Leu Glu Ala Glu Ala Gly Ile Ala Ala Asn Glu Pro Val Glu Met Ala 
225                 230                 235                 240 


Val Gln Glu Ala Ile Glu Lys Ala Val Tyr Asp Ile Ile Ile Glu Gly 
                245                 250                 255     


Val Lys Thr Gly Ile Trp Lys Pro Lys Asp Thr Lys Glu Phe Glu Lys 
            260                 265                 270         


Trp Leu Ser Lys Tyr Glu Asn Glu Leu Lys Glu Lys Thr Phe Ala Ser 
        275                 280                 285             


Glu Ser Ile Ser Lys Leu Ser Lys Gln Lys Glu Ser Lys Asp Ile Val 
    290                 295                 300                 


Lys Glu Leu Phe Lys Tyr 
305                 310 


