﻿                         序列表

<110>  北京伟德杰生物科技有限公司

<120>  长效化重组人白细胞介素2融合蛋白及其制备方法和应用

<130>  WO11358WD

<160>  5

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  133
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  1
Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      
Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          
Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              
Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  
Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  
Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      
Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         
Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             
Ile Ser Thr Leu Thr 
    130             

<210>  2
<211>  585
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  2
Asp Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu 
1               5                   10                  15      
Glu Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln 
            20                  25                  30          
Gln Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu 
        35                  40                  45              
Phe Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys 
    50                  55                  60                  
Ser Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu 
65                  70                  75                  80  
Arg Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro 
                85                  90                  95      
Glu Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu 
            100                 105                 110         
Pro Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His 
        115                 120                 125             
Asp Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg 
    130                 135                 140                 
Arg His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg 
145                 150                 155                 160 
Tyr Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala 
                165                 170                 175     
Cys Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser 
            180                 185                 190         
Ser Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu 
        195                 200                 205             
Arg Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro 
    210                 215                 220                 
Lys Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys 
225                 230                 235                 240 
Val His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp 
                245                 250                 255     
Arg Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser 
            260                 265                 270         
Ser Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His 
        275                 280                 285             
Cys Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser 
    290                 295                 300                 
Leu Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala 
305                 310                 315                 320 
Glu Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg 
                325                 330                 335     
Arg His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr 
            340                 345                 350         
Tyr Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu 
        355                 360                 365             
Cys Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro 
    370                 375                 380                 
Gln Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu 
385                 390                 395                 400 
Tyr Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro 
                405                 410                 415     
Gln Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys 
            420                 425                 430         
Val Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys 
        435                 440                 445             
Ala Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His 
    450                 455                 460                 
Glu Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser 
465                 470                 475                 480 
Leu Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr 
                485                 490                 495     
Tyr Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp 
            500                 505                 510         
Ile Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala 
        515                 520                 525             
Leu Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu 
    530                 535                 540                 
Lys Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys 
545                 550                 555                 560 
Ala Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val 
                565                 570                 575     
Ala Ala Ser Gln Ala Ala Leu Gly Leu 
            580                 585 

<210>  3
<211>  2187
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
gcaccaacta gcagcagcac taagaagacc cagctgcaac tcgaacatct gctgctcgac        60
ctgcaaatga tcctgaatgg gattaacaac tataagaatc ccaagctcac tcggatgctc       120
accttcaagt tctacatgcc caagaaagct actgaactca aacatctgca atgtctcgaa       180
gaggaactga agccactgga agaggtgctg aatctggccc aatctaagaa tttccacctg       240
aggcctaggg atctgataag caatatcaac gtgattgtgc tggagctgaa gggttctgaa       300
acaaccttta tgtgtgagta cgcagacgaa accgccacca tcgtcgagtt cctcaatcgg       360
tggatcacct tcgctcagag catcatctcc acactcacag gaggcggtgg gagtggtggt       420
ggagggtcag acgctcacaa gagcgaggtg gctcatcggt tcaaagacct cggcgaggag       480
aacttcaagg ccctggtcct gatcgccttt gcccagtacc tgcaacagtg tccctttgaa       540
gatcatgtca agctcgtcaa cgaggtgact gagttcgcca agacatgcgt cgcagacgag       600
agtgccgaga attgcgacaa atccctccac accctgtttg gcgataagct ctgtactgtc       660
gctactctga gggagacata cggcgagatg gctgactgct gcgccaagca ggagcccgag       720
cggaatgaat gctttctgca acacaaagac gacaatccta atctgccaag gctcgtcaga       780
ccagaggtgg atgtgatgtg taccgccttt catgataatg aggaaacctt tctcaagaag       840
tatctgtatg aaattgctag aaggcaccca tacttctatg ctcctgagct gctgttcttc       900
gccaaacgct acaaggctgc tttcactgaa tgctgccagg cagccgacaa agcagcctgc       960
ctcctgccaa agctcgacga actccgggac gaagggaagg cctcaagtgc caagcagaga      1020
ctgaagtgcg caagcctcca gaaattcgga gaaagggcat tcaaggcttg ggcagtggca      1080
aggctgtccc agaggtttcc taaggccgaa tttgccgaag tctctaaact ggtcacagac      1140
ctgaccaaag tgcataccga atgttgccac ggcgacctcc tggagtgcgc tgacgatagg      1200
gcagacctgg ccaaatacat ttgcgagaac caggatagta taagtagcaa actcaaggag      1260
tgctgtgaga agcctctcct ggagaagtcc cactgcatcg ccgaagtgga gaacgatgag      1320
atgccagctg acctccctag cctggcagcc gactttgtcg aatcaaagga cgtctgcaag      1380
aattacgccg aggcaaagga tgtgttcctg ggaatgtttc tgtacgagta tgccagaagg      1440
caccctgact attcagtcgt gctgctgctg cgcctggcca agacttatga aactaccctg      1500
gagaagtgtt gcgcagctgc cgacccacac gagtgttatg ccaaggtgtt cgatgagttc      1560
aagccactcg tcgaggagcc tcagaacctg atcaagcaga actgcgaact cttcgagcag      1620
ctgggagagt acaaattcca gaacgccctg ctggtgcggt acaccaagaa ggtgccacag      1680
gtgtctacac ctacactggt tgaagtgtcc agaaacctcg gcaaggtggg aagcaaatgc      1740
tgcaaacatc ccgaggcaaa gcggatgcca tgcgcagaag attacctctc tgtggtgctc      1800
aatcaactgt gcgtgctgca cgagaagacc cccgtcagtg acagagtgac caaatgctgc      1860
actgagagtc tggtcaatag acggccatgc ttcagtgccc tggaggtgga cgaaacatac      1920
gttcccaaag agttcaacgc cgaaactttc acctttcatg ctgacatttg taccctgtca      1980
gagaaggagc ggcagattaa gaaacaaact gccctggtgg aactggtgaa acacaagcct      2040
aaggcaacca aagaacaact gaaggccgtc atggacgatt tcgctgcctt cgtcgagaag      2100
tgttgtaagg ctgacgacaa ggagacatgt ttcgccgaag agggcaagaa actggttgct      2160
gccagccaag ctgcactggg actgtga                                          2187

<210>  4
<211>  728
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  4
Ala Pro Thr Ser Ser Ser Thr Lys Lys Thr Gln Leu Gln Leu Glu His 
1               5                   10                  15      
Leu Leu Leu Asp Leu Gln Met Ile Leu Asn Gly Ile Asn Asn Tyr Lys 
            20                  25                  30          
Asn Pro Lys Leu Thr Arg Met Leu Thr Phe Lys Phe Tyr Met Pro Lys 
        35                  40                  45              
Lys Ala Thr Glu Leu Lys His Leu Gln Cys Leu Glu Glu Glu Leu Lys 
    50                  55                  60                  
Pro Leu Glu Glu Val Leu Asn Leu Ala Gln Ser Lys Asn Phe His Leu 
65                  70                  75                  80  
Arg Pro Arg Asp Leu Ile Ser Asn Ile Asn Val Ile Val Leu Glu Leu 
                85                  90                  95      
Lys Gly Ser Glu Thr Thr Phe Met Cys Glu Tyr Ala Asp Glu Thr Ala 
            100                 105                 110         
Thr Ile Val Glu Phe Leu Asn Arg Trp Ile Thr Phe Ala Gln Ser Ile 
        115                 120                 125             
Ile Ser Thr Leu Thr Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
    130                 135                 140                 
Ala His Lys Ser Glu Val Ala His Arg Phe Lys Asp Leu Gly Glu Glu 
145                 150                 155                 160 
Asn Phe Lys Ala Leu Val Leu Ile Ala Phe Ala Gln Tyr Leu Gln Gln 
                165                 170                 175     
Cys Pro Phe Glu Asp His Val Lys Leu Val Asn Glu Val Thr Glu Phe 
            180                 185                 190         
Ala Lys Thr Cys Val Ala Asp Glu Ser Ala Glu Asn Cys Asp Lys Ser 
        195                 200                 205             
Leu His Thr Leu Phe Gly Asp Lys Leu Cys Thr Val Ala Thr Leu Arg 
    210                 215                 220                 
Glu Thr Tyr Gly Glu Met Ala Asp Cys Cys Ala Lys Gln Glu Pro Glu 
225                 230                 235                 240 
Arg Asn Glu Cys Phe Leu Gln His Lys Asp Asp Asn Pro Asn Leu Pro 
                245                 250                 255     
Arg Leu Val Arg Pro Glu Val Asp Val Met Cys Thr Ala Phe His Asp 
            260                 265                 270         
Asn Glu Glu Thr Phe Leu Lys Lys Tyr Leu Tyr Glu Ile Ala Arg Arg 
        275                 280                 285             
His Pro Tyr Phe Tyr Ala Pro Glu Leu Leu Phe Phe Ala Lys Arg Tyr 
    290                 295                 300                 
Lys Ala Ala Phe Thr Glu Cys Cys Gln Ala Ala Asp Lys Ala Ala Cys 
305                 310                 315                 320 
Leu Leu Pro Lys Leu Asp Glu Leu Arg Asp Glu Gly Lys Ala Ser Ser 
                325                 330                 335     
Ala Lys Gln Arg Leu Lys Cys Ala Ser Leu Gln Lys Phe Gly Glu Arg 
            340                 345                 350         
Ala Phe Lys Ala Trp Ala Val Ala Arg Leu Ser Gln Arg Phe Pro Lys 
        355                 360                 365             
Ala Glu Phe Ala Glu Val Ser Lys Leu Val Thr Asp Leu Thr Lys Val 
    370                 375                 380                 
His Thr Glu Cys Cys His Gly Asp Leu Leu Glu Cys Ala Asp Asp Arg 
385                 390                 395                 400 
Ala Asp Leu Ala Lys Tyr Ile Cys Glu Asn Gln Asp Ser Ile Ser Ser 
                405                 410                 415     
Lys Leu Lys Glu Cys Cys Glu Lys Pro Leu Leu Glu Lys Ser His Cys 
            420                 425                 430         
Ile Ala Glu Val Glu Asn Asp Glu Met Pro Ala Asp Leu Pro Ser Leu 
        435                 440                 445             
Ala Ala Asp Phe Val Glu Ser Lys Asp Val Cys Lys Asn Tyr Ala Glu 
    450                 455                 460                 
Ala Lys Asp Val Phe Leu Gly Met Phe Leu Tyr Glu Tyr Ala Arg Arg 
465                 470                 475                 480 
His Pro Asp Tyr Ser Val Val Leu Leu Leu Arg Leu Ala Lys Thr Tyr 
                485                 490                 495     
Glu Thr Thr Leu Glu Lys Cys Cys Ala Ala Ala Asp Pro His Glu Cys 
            500                 505                 510         
Tyr Ala Lys Val Phe Asp Glu Phe Lys Pro Leu Val Glu Glu Pro Gln 
        515                 520                 525             
Asn Leu Ile Lys Gln Asn Cys Glu Leu Phe Glu Gln Leu Gly Glu Tyr 
    530                 535                 540                 
Lys Phe Gln Asn Ala Leu Leu Val Arg Tyr Thr Lys Lys Val Pro Gln 
545                 550                 555                 560 
Val Ser Thr Pro Thr Leu Val Glu Val Ser Arg Asn Leu Gly Lys Val 
                565                 570                 575     
Gly Ser Lys Cys Cys Lys His Pro Glu Ala Lys Arg Met Pro Cys Ala 
            580                 585                 590         
Glu Asp Tyr Leu Ser Val Val Leu Asn Gln Leu Cys Val Leu His Glu 
        595                 600                 605             
Lys Thr Pro Val Ser Asp Arg Val Thr Lys Cys Cys Thr Glu Ser Leu 
    610                 615                 620                 
Val Asn Arg Arg Pro Cys Phe Ser Ala Leu Glu Val Asp Glu Thr Tyr 
625                 630                 635                 640 
Val Pro Lys Glu Phe Asn Ala Glu Thr Phe Thr Phe His Ala Asp Ile 
                645                 650                 655     
Cys Thr Leu Ser Glu Lys Glu Arg Gln Ile Lys Lys Gln Thr Ala Leu 
            660                 665                 670         
Val Glu Leu Val Lys His Lys Pro Lys Ala Thr Lys Glu Gln Leu Lys 
        675                 680                 685             
Ala Val Met Asp Asp Phe Ala Ala Phe Val Glu Lys Cys Cys Lys Ala 
    690                 695                 700                 
Asp Asp Lys Glu Thr Cys Phe Ala Glu Glu Gly Lys Lys Leu Val Ala 
705                 710                 715                 720 
Ala Ser Gln Ala Ala Leu Gly Leu 
                725             

<210>  5
<211>  16
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  5
Met Pro Leu Leu Leu Leu Leu Pro Leu Leu Trp Ala Gly Ala Leu Ala 
1               5                   10                  15      

