                         SEQUENCE LISTING

<110>  SUZHOU FUSE BIOSCIENCES LTD
 
<120>  IMMUNOCONJUGATES CONTAINING TNF-ALPHA AND RELATED METHODS AND 
       COMPOSITIONS THEREOF

<130>  14625-007-228

<140>  TBA
<141>  On even date herewith

<150>  PCT/CN2021/121318
<151>  2021-09-28

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  157
<212>  PRT
<213>  Homo sapiens

<220>
<223>  sequence of an exemplary human TNF-alpha

<400>  1

Val Arg Ser Ser Ser Arg Thr Pro Ser Asp Lys Pro Val Ala His Val 
1               5                   10                  15      


Val Ala Asn Pro Gln Ala Glu Gly Gln Leu Gln Trp Leu Asn Arg Arg 
            20                  25                  30          


Ala Asn Ala Leu Leu Ala Asn Gly Val Glu Leu Arg Asp Asn Gln Leu 
        35                  40                  45              


Val Val Pro Ser Glu Gly Leu Tyr Leu Ile Tyr Ser Gln Val Leu Phe 
    50                  55                  60                  


Lys Gly Gln Gly Cys Pro Ser Thr His Val Leu Leu Thr His Thr Ile 
65                  70                  75                  80  


Ser Arg Ile Ala Val Ser Tyr Gln Thr Lys Val Asn Leu Leu Ser Ala 
                85                  90                  95      


Ile Lys Ser Pro Cys Gln Arg Glu Thr Pro Glu Gly Ala Glu Ala Lys 
            100                 105                 110         


Pro Trp Tyr Glu Pro Ile Tyr Leu Gly Gly Val Phe Gln Leu Glu Lys 
        115                 120                 125             


Gly Asp Arg Leu Ser Ala Glu Ile Asn Arg Pro Asp Tyr Leu Asp Phe 
    130                 135                 140                 


Ala Glu Ser Gly Gln Val Tyr Phe Gly Ile Ile Ala Leu 
145                 150                 155         


<210>  2
<211>  454
<212>  PRT
<213>  Homo sapiens

<220>
<223>  sequence of an exemplary human TNFR1

<400>  2

Gly Leu Ser Thr Val Pro Asp Leu Leu Leu Pro Leu Val Leu Leu Glu 
1               5                   10                  15      


Leu Leu Val Gly Ile Tyr Pro Ser Gly Val Ile Gly Leu Val Pro His 
            20                  25                  30          


Leu Gly Asp Arg Glu Lys Arg Asp Ser Val Cys Pro Gln Gly Lys Tyr 
        35                  40                  45              


Ile His Pro Gln Asn Asn Ser Ile Cys Cys Thr Lys Cys His Lys Gly 
    50                  55                  60                  


Thr Tyr Leu Tyr Asn Asp Cys Pro Gly Pro Gly Gln Asp Thr Asp Cys 
65                  70                  75                  80  


Arg Glu Cys Glu Ser Gly Ser Phe Thr Ala Ser Glu Asn His Leu Arg 
                85                  90                  95      


His Cys Leu Ser Cys Ser Lys Cys Arg Lys Glu Met Gly Gln Val Glu 
            100                 105                 110         


Ile Ser Ser Cys Thr Val Asp Arg Asp Thr Val Cys Gly Cys Arg Lys 
        115                 120                 125             


Asn Gln Tyr Arg His Tyr Trp Ser Glu Asn Leu Phe Gln Cys Phe Asn 
    130                 135                 140                 


Cys Ser Leu Cys Leu Asn Gly Thr Val His Leu Ser Cys Gln Glu Lys 
145                 150                 155                 160 


Gln Asn Thr Val Cys Thr Cys His Ala Gly Phe Phe Leu Arg Glu Asn 
                165                 170                 175     


Glu Cys Val Ser Cys Ser Asn Cys Lys Lys Ser Leu Glu Cys Thr Lys 
            180                 185                 190         


Leu Cys Leu Pro Gln Ile Glu Asn Val Lys Gly Thr Glu Asp Ser Gly 
        195                 200                 205             


Thr Thr Val Leu Leu Pro Leu Val Ile Phe Phe Gly Leu Cys Leu Leu 
    210                 215                 220                 


Ser Leu Leu Phe Ile Gly Leu Met Tyr Arg Tyr Gln Arg Trp Lys Ser 
225                 230                 235                 240 


Lys Leu Tyr Ser Ile Val Cys Gly Lys Ser Thr Pro Glu Lys Glu Gly 
                245                 250                 255     


Glu Leu Glu Gly Thr Thr Thr Lys Pro Leu Ala Pro Asn Pro Ser Phe 
            260                 265                 270         


Ser Pro Thr Pro Gly Phe Thr Pro Thr Leu Gly Phe Ser Pro Val Pro 
        275                 280                 285             


Ser Ser Thr Phe Thr Ser Ser Ser Thr Tyr Thr Pro Gly Asp Cys Pro 
    290                 295                 300                 


Asn Phe Ala Ala Pro Arg Arg Glu Val Ala Pro Pro Tyr Gln Gly Ala 
305                 310                 315                 320 


Asp Pro Ile Leu Ala Thr Ala Leu Ala Ser Asp Pro Ile Pro Asn Pro 
                325                 330                 335     


Leu Gln Lys Trp Glu Asp Ser Ala His Lys Pro Gln Ser Leu Asp Thr 
            340                 345                 350         


Asp Asp Pro Ala Thr Leu Tyr Ala Val Val Glu Asn Val Pro Pro Leu 
        355                 360                 365             


Arg Trp Lys Glu Phe Val Arg Arg Leu Gly Leu Ser Asp His Glu Ile 
    370                 375                 380                 


Asp Arg Leu Glu Leu Gln Asn Gly Arg Cys Leu Arg Glu Ala Gln Tyr 
385                 390                 395                 400 


Ser Met Leu Ala Thr Trp Arg Arg Arg Thr Pro Arg Arg Glu Ala Thr 
                405                 410                 415     


Leu Glu Leu Leu Gly Arg Val Leu Arg Asp Met Asp Leu Leu Gly Cys 
            420                 425                 430         


Leu Glu Asp Ile Glu Glu Ala Leu Cys Gly Pro Ala Ala Leu Pro Pro 
        435                 440                 445             


Ala Pro Ser Leu Leu Arg 
    450                 


<210>  3
<211>  460
<212>  PRT
<213>  Homo sapiens

<220>
<223>  sequence of an exemplary human TNFR2

<400>  3

Ala Pro Val Ala Val Trp Ala Ala Leu Ala Val Gly Leu Glu Leu Trp 
1               5                   10                  15      


Ala Ala Ala His Ala Leu Pro Ala Gln Val Ala Phe Thr Pro Tyr Ala 
            20                  25                  30          


Pro Glu Pro Gly Ser Thr Cys Arg Leu Arg Glu Tyr Tyr Asp Gln Thr 
        35                  40                  45              


Ala Gln Met Cys Cys Ser Lys Cys Ser Pro Gly Gln His Ala Lys Val 
    50                  55                  60                  


Phe Cys Thr Lys Thr Ser Asp Thr Val Cys Asp Ser Cys Glu Asp Ser 
65                  70                  75                  80  


Thr Tyr Thr Gln Leu Trp Asn Trp Val Pro Glu Cys Leu Ser Cys Gly 
                85                  90                  95      


Ser Arg Cys Ser Ser Asp Gln Val Glu Thr Gln Ala Cys Thr Arg Glu 
            100                 105                 110         


Gln Asn Arg Ile Cys Thr Cys Arg Pro Gly Trp Tyr Cys Ala Leu Ser 
        115                 120                 125             


Lys Gln Glu Gly Cys Arg Leu Cys Ala Pro Leu Arg Lys Cys Arg Pro 
    130                 135                 140                 


Gly Phe Gly Val Ala Arg Pro Gly Thr Glu Thr Ser Asp Val Val Cys 
145                 150                 155                 160 


Lys Pro Cys Ala Pro Gly Thr Phe Ser Asn Thr Thr Ser Ser Thr Asp 
                165                 170                 175     


Ile Cys Arg Pro His Gln Ile Cys Asn Val Val Ala Ile Pro Gly Asn 
            180                 185                 190         


Ala Ser Met Asp Ala Val Cys Thr Ser Thr Ser Pro Thr Arg Ser Met 
        195                 200                 205             


Ala Pro Gly Ala Val His Leu Pro Gln Pro Val Ser Thr Arg Ser Gln 
    210                 215                 220                 


His Thr Gln Pro Thr Pro Glu Pro Ser Thr Ala Pro Ser Thr Ser Phe 
225                 230                 235                 240 


Leu Leu Pro Met Gly Pro Ser Pro Pro Ala Glu Gly Ser Thr Gly Asp 
                245                 250                 255     


Phe Ala Leu Pro Val Gly Leu Ile Val Gly Val Thr Ala Leu Gly Leu 
            260                 265                 270         


Leu Ile Ile Gly Val Val Asn Cys Val Ile Met Thr Gln Val Lys Lys 
        275                 280                 285             


Lys Pro Leu Cys Leu Gln Arg Glu Ala Lys Val Pro His Leu Pro Ala 
    290                 295                 300                 


Asp Lys Ala Arg Gly Thr Gln Gly Pro Glu Gln Gln His Leu Leu Ile 
305                 310                 315                 320 


Thr Ala Pro Ser Ser Ser Ser Ser Ser Leu Glu Ser Ser Ala Ser Ala 
                325                 330                 335     


Leu Asp Arg Arg Ala Pro Thr Arg Asn Gln Pro Gln Ala Pro Gly Val 
            340                 345                 350         


Glu Ala Ser Gly Ala Gly Glu Ala Arg Ala Ser Thr Gly Ser Ser Asp 
        355                 360                 365             


Ser Ser Pro Gly Gly His Gly Thr Gln Val Asn Val Thr Cys Ile Val 
    370                 375                 380                 


Asn Val Cys Ser Ser Ser Asp His Ser Ser Gln Cys Ser Ser Gln Ala 
385                 390                 395                 400 


Ser Ser Thr Met Gly Asp Thr Asp Ser Ser Pro Ser Glu Ser Pro Lys 
                405                 410                 415     


Asp Glu Gln Val Pro Phe Ser Lys Glu Glu Cys Ala Phe Arg Ser Gln 
            420                 425                 430         


Leu Glu Thr Pro Glu Thr Leu Leu Gly Ser Thr Glu Glu Lys Pro Leu 
        435                 440                 445             


Pro Leu Gly Val Pro Asp Ala Gly Met Lys Pro Ser 
    450                 455                 460 


<210>  4
<211>  760
<212>  PRT
<213>  Homo sapiens

<220>
<223>  sequence of an exemplary human FAP

<400>  4

Met Lys Thr Trp Val Lys Ile Val Phe Gly Val Ala Thr Ser Ala Val 
1               5                   10                  15      


Leu Ala Leu Leu Val Met Cys Ile Val Leu Arg Pro Ser Arg Val His 
            20                  25                  30          


Asn Ser Glu Glu Asn Thr Met Arg Ala Leu Thr Leu Lys Asp Ile Leu 
        35                  40                  45              


Asn Gly Thr Phe Ser Tyr Lys Thr Phe Phe Pro Asn Trp Ile Ser Gly 
    50                  55                  60                  


Gln Glu Tyr Leu His Gln Ser Ala Asp Asn Asn Ile Val Leu Tyr Asn 
65                  70                  75                  80  


Ile Glu Thr Gly Gln Ser Tyr Thr Ile Leu Ser Asn Arg Thr Met Lys 
                85                  90                  95      


Ser Val Asn Ala Ser Asn Tyr Gly Leu Ser Pro Asp Arg Gln Phe Val 
            100                 105                 110         


Tyr Leu Glu Ser Asp Tyr Ser Lys Leu Trp Arg Tyr Ser Tyr Thr Ala 
        115                 120                 125             


Thr Tyr Tyr Ile Tyr Asp Leu Ser Asn Gly Glu Phe Val Arg Gly Asn 
    130                 135                 140                 


Glu Leu Pro Arg Pro Ile Gln Tyr Leu Cys Trp Ser Pro Val Gly Ser 
145                 150                 155                 160 


Lys Leu Ala Tyr Val Tyr Gln Asn Asn Ile Tyr Leu Lys Gln Arg Pro 
                165                 170                 175     


Gly Asp Pro Pro Phe Gln Ile Thr Phe Asn Gly Arg Glu Asn Lys Ile 
            180                 185                 190         


Phe Asn Gly Ile Pro Asp Trp Val Tyr Glu Glu Glu Met Leu Ala Thr 
        195                 200                 205             


Lys Tyr Ala Leu Trp Trp Ser Pro Asn Gly Lys Phe Leu Ala Tyr Ala 
    210                 215                 220                 


Glu Phe Asn Asp Thr Asp Ile Pro Val Ile Ala Tyr Ser Tyr Tyr Gly 
225                 230                 235                 240 


Asp Glu Gln Tyr Pro Arg Thr Ile Asn Ile Pro Tyr Pro Lys Ala Gly 
                245                 250                 255     


Ala Lys Asn Pro Val Val Arg Ile Phe Ile Ile Asp Thr Thr Tyr Pro 
            260                 265                 270         


Ala Tyr Val Gly Pro Gln Glu Val Pro Val Pro Ala Met Ile Ala Ser 
        275                 280                 285             


Ser Asp Tyr Tyr Phe Ser Trp Leu Thr Trp Val Thr Asp Glu Arg Val 
    290                 295                 300                 


Cys Leu Gln Trp Leu Lys Arg Val Gln Asn Val Ser Val Leu Ser Ile 
305                 310                 315                 320 


Cys Asp Phe Arg Glu Asp Trp Gln Thr Trp Asp Cys Pro Lys Thr Gln 
                325                 330                 335     


Glu His Ile Glu Glu Ser Arg Thr Gly Trp Ala Gly Gly Phe Phe Val 
            340                 345                 350         


Ser Thr Pro Val Phe Ser Tyr Asp Ala Ile Ser Tyr Tyr Lys Ile Phe 
        355                 360                 365             


Ser Asp Lys Asp Gly Tyr Lys His Ile His Tyr Ile Lys Asp Thr Val 
    370                 375                 380                 


Glu Asn Ala Ile Gln Ile Thr Ser Gly Lys Trp Glu Ala Ile Asn Ile 
385                 390                 395                 400 


Phe Arg Val Thr Gln Asp Ser Leu Phe Tyr Ser Ser Asn Glu Phe Glu 
                405                 410                 415     


Glu Tyr Pro Gly Arg Arg Asn Ile Tyr Arg Ile Ser Ile Gly Ser Tyr 
            420                 425                 430         


Pro Pro Ser Lys Lys Cys Val Thr Cys His Leu Arg Lys Glu Arg Cys 
        435                 440                 445             


Gln Tyr Tyr Thr Ala Ser Phe Ser Asp Tyr Ala Lys Tyr Tyr Ala Leu 
    450                 455                 460                 


Val Cys Tyr Gly Pro Gly Ile Pro Ile Ser Thr Leu His Asp Gly Arg 
465                 470                 475                 480 


Thr Asp Gln Glu Ile Lys Ile Leu Glu Glu Asn Lys Glu Leu Glu Asn 
                485                 490                 495     


Ala Leu Lys Asn Ile Gln Leu Pro Lys Glu Glu Ile Lys Lys Leu Glu 
            500                 505                 510         


Val Asp Glu Ile Thr Leu Trp Tyr Lys Met Ile Leu Pro Pro Gln Phe 
        515                 520                 525             


Asp Arg Ser Lys Lys Tyr Pro Leu Leu Ile Gln Val Tyr Gly Gly Pro 
    530                 535                 540                 


Cys Ser Gln Ser Val Arg Ser Val Phe Ala Val Asn Trp Ile Ser Tyr 
545                 550                 555                 560 


Leu Ala Ser Lys Glu Gly Met Val Ile Ala Leu Val Asp Gly Arg Gly 
                565                 570                 575     


Thr Ala Phe Gln Gly Asp Lys Leu Leu Tyr Ala Val Tyr Arg Lys Leu 
            580                 585                 590         


Gly Val Tyr Glu Val Glu Asp Gln Ile Thr Ala Val Arg Lys Phe Ile 
        595                 600                 605             


Glu Met Gly Phe Ile Asp Glu Lys Arg Ile Ala Ile Trp Gly Trp Ser 
    610                 615                 620                 


Tyr Gly Gly Tyr Val Ser Ser Leu Ala Leu Ala Ser Gly Thr Gly Leu 
625                 630                 635                 640 


Phe Lys Cys Gly Ile Ala Val Ala Pro Val Ser Ser Trp Glu Tyr Tyr 
                645                 650                 655     


Ala Ser Val Tyr Thr Glu Arg Phe Met Gly Leu Pro Thr Lys Asp Asp 
            660                 665                 670         


Asn Leu Glu His Tyr Lys Asn Ser Thr Val Met Ala Arg Ala Glu Tyr 
        675                 680                 685             


Phe Arg Asn Val Asp Tyr Leu Leu Ile His Gly Thr Ala Asp Asp Asn 
    690                 695                 700                 


Val His Phe Gln Asn Ser Ala Gln Ile Ala Lys Ala Leu Val Asn Ala 
705                 710                 715                 720 


Gln Val Asp Phe Gln Ala Met Trp Tyr Ser Asp Gln Asn His Gly Leu 
                725                 730                 735     


Ser Gly Leu Ser Thr Asn His Leu Tyr Thr His Met Thr His Phe Leu 
            740                 745                 750         


Lys Gln Cys Phe Ser Leu Ser Asp 
        755                 760 


<210>  5
<211>  322
<212>  PRT
<213>  Homo sapiens

<220>
<223>  sequence of an exemplary human Trop-2 

<400>  5

Ala Arg Gly Pro Gly Leu Ala Pro Pro Pro Leu Arg Leu Pro Leu Leu 
1               5                   10                  15      


Leu Leu Val Leu Ala Ala Val Thr Gly His Thr Ala Ala Gln Asp Asn 
            20                  25                  30          


Cys Thr Cys Pro Thr Asn Lys Met Thr Val Cys Ser Pro Asp Gly Pro 
        35                  40                  45              


Gly Gly Arg Cys Gln Cys Arg Ala Leu Gly Ser Gly Met Ala Val Asp 
    50                  55                  60                  


Cys Ser Thr Leu Thr Ser Lys Cys Leu Leu Leu Lys Ala Arg Met Ser 
65                  70                  75                  80  


Ala Pro Lys Asn Ala Arg Thr Leu Val Arg Pro Ser Glu His Ala Leu 
                85                  90                  95      


Val Asp Asn Asp Gly Leu Tyr Asp Pro Asp Cys Asp Pro Glu Gly Arg 
            100                 105                 110         


Phe Lys Ala Arg Gln Cys Asn Gln Thr Ser Val Cys Trp Cys Val Asn 
        115                 120                 125             


Ser Val Gly Val Arg Arg Thr Asp Lys Gly Asp Leu Ser Leu Arg Cys 
    130                 135                 140                 


Asp Glu Leu Val Arg Thr His His Ile Leu Ile Asp Leu Arg His Arg 
145                 150                 155                 160 


Pro Thr Ala Gly Ala Phe Asn His Ser Asp Leu Asp Ala Glu Leu Arg 
                165                 170                 175     


Arg Leu Phe Arg Glu Arg Tyr Arg Leu His Pro Lys Phe Val Ala Ala 
            180                 185                 190         


Val His Tyr Glu Gln Pro Thr Ile Gln Ile Glu Leu Arg Gln Asn Thr 
        195                 200                 205             


Ser Gln Lys Ala Ala Gly Asp Val Asp Ile Gly Asp Ala Ala Tyr Tyr 
    210                 215                 220                 


Phe Glu Arg Asp Ile Lys Gly Glu Ser Leu Phe Gln Gly Arg Gly Gly 
225                 230                 235                 240 


Leu Asp Leu Arg Val Arg Gly Glu Pro Leu Gln Val Glu Arg Thr Leu 
                245                 250                 255     


Ile Tyr Tyr Leu Asp Glu Ile Pro Pro Lys Phe Ser Met Lys Arg Leu 
            260                 265                 270         


Thr Ala Gly Leu Ile Ala Val Ile Val Val Val Val Val Ala Leu Val 
        275                 280                 285             


Ala Gly Met Ala Val Leu Val Ile Thr Asn Arg Arg Lys Ser Gly Lys 
    290                 295                 300                 


Tyr Lys Lys Val Glu Ile Lys Glu Leu Gly Glu Leu Arg Lys Glu Pro 
305                 310                 315                 320 


Ser Leu 
        


<210>  6
<211>  227
<212>  PRT
<213>  Homo sapiens

<220>
<223>  a native human IgG1 Fc region amino acid sequence

<400>  6

Asp Lys Thr His Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly 
1               5                   10                  15      


Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met 
            20                  25                  30          


Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His 
        35                  40                  45              


Glu Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val 
    50                  55                  60                  


His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr 
65                  70                  75                  80  


Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly 
                85                  90                  95      


Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile 
            100                 105                 110         


Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val 
        115                 120                 125             


Tyr Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser 
    130                 135                 140                 


Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu 
145                 150                 155                 160 


Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro 
                165                 170                 175     


Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val 
            180                 185                 190         


Asp Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met 
        195                 200                 205             


His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser 
    210                 215                 220                 


Pro Gly Lys 
225         


<210>  7
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<221>  REPEAT
<222>  (1)..(5)
<223>  repeats of G4S fragment at the specified length

<400>  7

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  




