                         SEQUENCE LISTING

<110>  Wilmar International Ltd.
 
<120>  METHOD OF EXTRACTION AND FERMENTATION

<130>  77142SG

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  794
<212>  DNA
<213>  Artificial sequence

<220>
<223>  DNA sequence for ferulic acid esterase gene from Aspergillus 
       niger (AnFAE)


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  EcoRI restriction site

<220>
<221>  misc_feature
<222>  (787)..(794)
<223>  NotI restriction site

<400>  1
gaattcgctt ccactcaggg tatttccgag gacttgtaca acagattggt tgagatggcc       60

actatctccc aagctgctta cgctgacttg tgtaacatcc catccaccat catcaagggt      120

gagaaaatct acaacgccca gaccgacatt aacggttgga tcttgagaga tgacacctcc      180

aaagagatca tcaccgtttt cagaggtact ggttccgaca ccaacttgca gttggacact      240

aactacactc tgaccccatt cgacactttg ccacagtgta acgactgtga agttcacggt      300

ggttactaca tcggttggat ttccgttcaa gaccaggttg agtccttggt taagcaacag      360

gcttctcagt acccagacta cgctttgact gttactggtc actctttggg tgcttctatg      420

gctgctttga cagctgctca attgtccgct acttacgaca acgttagact gtacactttc      480

ggtgagccaa gatccggtaa ccaggctttt gcttcttaca tgaacgacgc tttccaggtg      540

tcctctccag agactactca gtacttcaga gttacccact ccaacgacgg tatcccaaat      600

ttgccaccag ctgaacaagg ttacgctcat ggtggtgttg aatactggtc tgttgaccca      660

tactccgctc agaacacttt cgtctgtact ggtgacgagg ttcagtgttg tgaagctcaa      720

ggtggacaag gtgttaacga cgctcacact acttacttcg gtatgacttc cggtgcttgt      780

acttgggcgg ccgc                                                        794


<210>  2
<211>  260
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Protein sequence of the synthesized gene for ferulic acid 
       esterase gene from Aspergillus niger (AnFAE)

<400>  2

Ala Ser Thr Gln Gly Ile Ser Glu Asp Leu Tyr Asn Arg Leu Val Glu 
1               5                   10                  15      


Met Ala Thr Ile Ser Gln Ala Ala Tyr Ala Asp Leu Cys Asn Ile Pro 
            20                  25                  30          


Ser Thr Ile Ile Lys Gly Glu Lys Ile Tyr Asn Ala Gln Thr Asp Ile 
        35                  40                  45              


Asn Gly Trp Ile Leu Arg Asp Asp Thr Ser Lys Glu Ile Ile Thr Val 
    50                  55                  60                  


Phe Arg Gly Thr Gly Ser Asp Thr Asn Leu Gln Leu Asp Thr Asn Tyr 
65                  70                  75                  80  


Thr Leu Thr Pro Phe Asp Thr Leu Pro Gln Cys Asn Asp Cys Glu Val 
                85                  90                  95      


His Gly Gly Tyr Tyr Ile Gly Trp Ile Ser Val Gln Asp Gln Val Glu 
            100                 105                 110         


Ser Leu Val Lys Gln Gln Ala Ser Gln Tyr Pro Asp Tyr Ala Leu Thr 
        115                 120                 125             


Val Thr Gly His Ser Leu Gly Ala Ser Met Ala Ala Leu Thr Ala Ala 
    130                 135                 140                 


Gln Leu Ser Ala Thr Tyr Asp Asn Val Arg Leu Tyr Thr Phe Gly Glu 
145                 150                 155                 160 


Pro Arg Ser Gly Asn Gln Ala Phe Ala Ser Tyr Met Asn Asp Ala Phe 
                165                 170                 175     


Gln Val Ser Ser Pro Glu Thr Thr Gln Tyr Phe Arg Val Thr His Ser 
            180                 185                 190         


Asn Asp Gly Ile Pro Asn Leu Pro Pro Ala Glu Gln Gly Tyr Ala His 
        195                 200                 205             


Gly Gly Val Glu Tyr Trp Ser Val Asp Pro Tyr Ser Ala Gln Asn Thr 
    210                 215                 220                 


Phe Val Cys Thr Gly Asp Glu Val Gln Cys Cys Glu Ala Gln Gly Gly 
225                 230                 235                 240 


Gln Gly Val Asn Asp Ala His Thr Thr Tyr Phe Gly Met Thr Ser Gly 
                245                 250                 255     


Ala Cys Thr Trp 
            260 


<210>  3
<211>  17
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WIC1P2336 (universal primer for 16S rRNA analysis)

<400>  3
cctacgggag gcagcag                                                      17


<210>  4
<211>  20
<212>  DNA
<213>  Artificial sequence

<220>
<223>  WIC1P2337 (universal primer for 16S rRNA analysis)

<400>  4
ggactachvg ggtwtctaat                                                   20


