                         SEQUENCE LISTING

<110>  ɶ̼Ƽ޹˾
 
<120>  ׵׵ͻ塢׿׼Ӧ

<130>  PCT21125CN

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  277
<212>  PRT
<213>  Gammaproteobacteria bacterium MFB021

<400>  1

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Gln 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Ala Pro Ala Ser Thr Phe Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  2
<211>  834
<212>  DNA
<213>  Gammaproteobacteria bacterium MFB021

<400>  2
atgtacaaac tcatcgctgc ggtagcgact gccgcactgc tctccggctg tgccgggatg       60

gtcgccaatg tcgacggtct cgagggaaaa ccgcccacgc tgacgccccg cggggcgacc      120

tggcaggatc tcaaggcgct gcctcgcccc gccggcaaga ttctcgccgc ggtctacgat      180

tttcgtgatc agaccgggca gtaccggccg gctcctgcca gtaccttctc caccgctgtc      240

actcagggcg ccgcggcaat gctgaccggt gcgctggccg actccggctg gttcacgccg      300

ctggagcggg tcggtctgca gaatctgctg accgagcggc gtatcatccg cgccgagcag      360

gagcgcttca atcggccgga caacctgccc tcgctgcgct ccgcctcggt gctgctggag      420

ggcggtatca tcgcctacga atccaacgtg cgcaccggcg ggctgggcgc cgagtacttc      480

ggcatcggcg cctcggggca gtatcaggtc gaccaggtga cgatcaatct gcgcgcggtc      540

gagatctcca gcggagaggt gctggccaac gtcaccacca ccaagaccat ctactccaag      600

gagatccgcg ccggcgtatt ccgcttcatc gatttccgcc gcctgctcga ggcagaagct      660

ggcctgacta ccaacgaacc ggtacagatg gcggtgatgt cggcaatcga gtccgcggtc      720

atccatctga tcgccgaggg cgtgcaggat agactctgga atctcgccga cggcgtgccg      780

ctggagaaga cagtacttgc cgactacctg aacgagagcg tgccgatgat gtag            834


<210>  3
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  3
tttttttttt                                                              10


<210>  4
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  4
aatgtacttc gttcagttac gtattgct                                          28


<210>  5
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  5
gcaatacgta actgaacgaa gttcactatc gcattctcat ga                          42


<210>  6
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  6
tcatgagaat gcgatagtga                                                   20


<210>  7
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  7
aaaaaaaaaa aaaaaaaaaa aaaaaaaa                                          28


<210>  8
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  8
aaaaaaaaaa aa                                                           12


<210>  9
<211>  132
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  9
aaaaaaaaaa aaaatctctg aatctctgaa tctctgaatc tctaaaaaaa aaaaagaaaa       60

aaaaaaaaca aaaaaaaaaa ataaaaaaaa aaaaagcaat acgtaactga acgaagtaca      120

ttaaaaaaaa aa                                                          132


<210>  10
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  10
atcctttttt ttttaatgta cttcgttcag ttacgtattg ct                          42


<210>  11
<211>  94
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  11
tttttttttt ttattttttt tttttgtttt ttttttttct tttttttttt tagagattca       60

gagattcaga gattcagaga tttttttttt tttt                                   94


<210>  12
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  12
tttttttttt tt                                                           12


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  part of BS7-4C3-PLT

<400>  13
tttttttttt tttttttttt tttttttt                                          28


<210>  14
<211>  441
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  14

Met Ile Thr Ile Asp Gln Leu Thr Glu Gly Gln Phe Asp Ser Leu Gln 
1               5                   10                  15      


Arg Ala Lys Val Leu Ile Gln Glu Ala Thr Lys Asn Asp Gly Asn Trp 
            20                  25                  30          


Asn His Arg Thr Lys His Leu Thr Ile Asn Gly Pro Ala Gly Thr Gly 
        35                  40                  45              


Lys Thr Thr Met Met Lys Phe Leu Val Ser Trp Leu Arg Asp Glu Gly 
    50                  55                  60                  


Ile Thr Gly Val Ala Leu Ala Ala Pro Thr His Ala Ala Lys Lys Val 
65                  70                  75                  80  


Leu Ala Asn Ala Val Gly Glu Glu Val Ser Thr Ile His Ser Ile Leu 
                85                  90                  95      


Lys Ile Asn Pro Thr Thr Tyr Glu Cys Lys Gln Phe Phe Glu Gln Ser 
            100                 105                 110         


Ala Pro Pro Asp Leu Ser Lys Ile Arg Ile Leu Ile Cys Glu Glu Cys 
        115                 120                 125             


Ser Phe Tyr Asp Ile Lys Leu Phe Glu Ile Leu Met Asn Ser Ile Gln 
    130                 135                 140                 


Pro Trp Thr Ile Ile Ile Gly Ile Gly Asp Arg Ala Gln Leu Arg Pro 
145                 150                 155                 160 


Ala Asp Asp Lys Gly Ile Ser Arg Phe Phe Thr Asp Gln Arg Phe Glu 
                165                 170                 175     


Gln Thr Tyr Leu Thr Glu Ile Lys Arg Ser Asn Met Pro Ile Ile Glu 
            180                 185                 190         


Val Ala Thr Glu Ile Arg Asn Gly Gly Trp Ile Arg Glu Asn Ile Ile 
        195                 200                 205             


Asp Asp Leu Gly Val Lys Gln Asp Lys Ser Val Ser Glu Phe Met Thr 
    210                 215                 220                 


Asn Tyr Phe Lys Val Val Lys Ser Ile Asp Asp Leu Tyr Glu Thr Arg 
225                 230                 235                 240 


Met Tyr Ala Tyr Thr Asn Asn Ser Val Asp Thr Leu Asn Lys Ile Ile 
                245                 250                 255     


Arg Lys Lys Leu Tyr Glu Thr Glu Gln Asp Phe Ile Val Gly Glu Pro 
            260                 265                 270         


Ile Val Met Gln Glu Pro Leu Ile Arg Asp Ile Asn Tyr Glu Gly Lys 
        275                 280                 285             


Arg Phe Gln Glu Ile Val Phe Asn Asn Gly Glu Tyr Leu Glu Val Ser 
    290                 295                 300                 


Glu Ile Lys Pro Met Glu Ser Val Leu Lys Cys Arg Asn Ile Asp Tyr 
305                 310                 315                 320 


Gln Leu Val Leu His Tyr Tyr Gln Leu Lys Val Lys Ser Ile Asp Thr 
                325                 330                 335     


Gly Glu Ser Gly Leu Ile Asn Thr Ile Ser Asp Lys Asn Glu Leu Asn 
            340                 345                 350         


Lys Phe Tyr Met Phe Leu Gly Lys Val Cys Gln Asp Tyr Lys Ser Gly 
        355                 360                 365             


Thr Ile Lys Ala Phe Trp Asp Asp Phe Trp Lys Ile Lys Asn Asn Tyr 
    370                 375                 380                 


His Arg Val Lys Pro Leu Pro Val Ser Thr Ile His Lys Gly Gln Gly 
385                 390                 395                 400 


Ser Thr Val Asp Asn Ser Phe Leu Tyr Thr Pro Cys Ile Thr Lys Tyr 
                405                 410                 415     


Ala Glu Pro Asp Leu Ala Ser Gln Leu Leu Tyr Val Gly Val Thr Arg 
            420                 425                 430         


Ala Arg His Asn Val Asn Phe Val Gly 
        435                 440     


<210>  15
<211>  1326
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  helicase

<400>  15
atgatcacca tcgaccagct gaccgaaggt cagttcgact ctctgcagcg tgctaaagtt       60

ctgatccagg aagctaccaa aaacgacggt aactggaacc accgtaccaa acacctgacc      120

atcaacggtc cggctggtac cggtaaaacc accatgatga aattcctggt ttcttggctg      180

cgtgacgaag gtatcaccgg tgttgctctg gctgctccga cccacgctgc taaaaaagtt      240

ctggctaacg ctgttggtga agaagtttct accatccact ctatcctgaa aatcaacccg      300

accacctacg aatgcaaaca gttcttcgaa cagtctgctc cgccggacct gtctaaaatc      360

cgtatcctga tctgcgaaga atgctctttc tacgacatca aactgttcga aatcctgatg      420

aactctatcc agccgtggac catcatcatc ggtatcggtg accgtgctca gctgcgtccg      480

gctgacgaca aaggtatctc tcgtttcttc accgaccagc gtttcgaaca gacctacctg      540

accgaaatca aacgttctaa catgccgatc atcgaagttg ctaccgaaat ccgtaacggt      600

ggttggattc gtgaaaacat catcgacgac ctgggtgtta aacaggacaa atctgtttct      660

gaatttatga ccaactactt caaagttgtt aaatctatcg acgacctgta cgaaacccgt      720

atgtacgctt acaccaacaa ctctgttgac accctgaaca aaatcatccg taaaaaactg      780

tacgaaaccg aacaggactt catcgttggt gaaccgatcg ttatgcagga accgctgatc      840

cgtgacatca actacgaagg taaacgtttc caggaaatcg ttttcaacaa cggtgaatac      900

ctggaagttt ctgaaatcaa accgatggaa tctgttctga aatgccgtaa catcgactac      960

cagctggttc tgcactacta ccagctgaaa gttaaatcta tcgacaccgg tgaatctggt     1020

ctgatcaaca ccatctctga caaaaacgaa ctgaacaaat tctacatgtt cctgggtaaa     1080

gtttgccagg actacaaatc tggtaccatc aaagcgttct gggacgactt ctggaaaatc     1140

aaaaacaact accaccgtgt taaaccgctg ccggtttcta ccatccacaa aggtcagggt     1200

tctaccgttg acaactcttt cctgtacacc ccgtgcatca ccaaatacgc tgaaccggac     1260

ctggcttctc agctgctgta cgttggtgtt acccgtgctc gtcacaacgt taacttcgtt     1320

ggttaa                                                                1326


<210>  16
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 1

<400>  16

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Leu 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Leu Pro Ala Ser Ala Asn Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  17
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 2

<400>  17

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Gln 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Ala Pro Ala Ser Ser Asn Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  18
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 3

<400>  18

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Gln 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Leu Pro Ala Ser Ser Gln Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  19
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 4

<400>  19

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Gln 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Ala Pro Ala Ser Asn Gln Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  20
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 5

<400>  20

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Leu 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Leu Pro Ala Ser Ser Tyr Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  21
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 6

<400>  21

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Leu 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Leu Pro Ala Ser Ala Asn Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Glu Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Glu Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Glu Ala Glu Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


<210>  22
<211>  277
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  mutant 7

<400>  22

Met Tyr Lys Leu Ile Ala Ala Val Ala Thr Ala Ala Leu Leu Ser Gly 
1               5                   10                  15      


Cys Ala Gly Met Val Ala Asn Val Asp Gly Leu Glu Gly Lys Pro Pro 
            20                  25                  30          


Thr Leu Thr Pro Arg Gly Ala Thr Trp Gln Asp Leu Lys Ala Leu Pro 
        35                  40                  45              


Arg Pro Ala Gly Lys Ile Leu Ala Ala Val Tyr Asp Phe Arg Asp Leu 
    50                  55                  60                  


Thr Gly Gln Tyr Arg Pro Tyr Pro Ala Ser Ala Asn Ser Thr Ala Val 
65                  70                  75                  80  


Thr Gln Gly Ala Ala Ala Met Leu Thr Gly Ala Leu Ala Asp Ser Gly 
                85                  90                  95      


Trp Phe Thr Pro Leu Glu Arg Val Gly Leu Gln Asn Leu Leu Thr Glu 
            100                 105                 110         


Arg Arg Ile Ile Arg Ala Glu Gln Glu Arg Phe Asn Arg Pro Asp Asn 
        115                 120                 125             


Leu Pro Ser Leu Arg Ser Ala Ser Val Leu Leu Glu Gly Gly Ile Ile 
    130                 135                 140                 


Ala Tyr Glu Ser Asn Val Arg Thr Gly Gly Leu Gly Ala Arg Tyr Phe 
145                 150                 155                 160 


Gly Ile Gly Ala Ser Gly Gln Tyr Gln Val Asp Gln Val Thr Ile Asn 
                165                 170                 175     


Leu Arg Ala Val Glu Ile Ser Ser Gly Glu Val Leu Ala Asn Val Thr 
            180                 185                 190         


Thr Thr Lys Thr Ile Tyr Ser Lys Lys Ile Arg Ala Gly Val Phe Arg 
        195                 200                 205             


Phe Ile Asp Phe Arg Arg Leu Leu Gln Ala Arg Ala Gly Leu Thr Thr 
    210                 215                 220                 


Asn Glu Pro Val Gln Met Ala Val Met Ser Ala Ile Glu Ser Ala Val 
225                 230                 235                 240 


Ile His Leu Ile Ala Glu Gly Val Gln Asp Arg Leu Trp Asn Leu Ala 
                245                 250                 255     


Asp Gly Val Pro Leu Glu Lys Thr Val Leu Ala Asp Tyr Leu Asn Glu 
            260                 265                 270         


Ser Val Pro Met Met 
        275         


