                         SEQUENCE LISTING

<110>  National Health Research Institutes
 
<120>  METHOD OF ENDING A DOUBLE-STRANDED DNA WITH AT LEAST ONE 
       STEM-LOOP AND A KIT THEREOF

<130>  5382/0357PWO1

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16S-Takara-For

<400>  1
aagcagtggt atcaacgcag agtagrgtty gatymtggct cag                         43


<210>  2
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16S-RCA5-RevT

<400>  2
cagacgtgtg ctcttccgat ctrgytacct tgttacgact t                           41


<210>  3
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  one of the two looping oligos


<220>
<221>  stem_loop
<222>  (1)..(53)

<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  linked with phosphorothiolate bonds

<400>  3
ggccagcagc agaggaggac gctgctggcc aagcagtggt atcaacgcag agt              53


<210>  4
<211>  61
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other one of the two looping oligos


<220>
<221>  stem_loop
<222>  (1)..(61)

<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  linked with phosphorothiolate bonds

<400>  4
gaagtgcgct gtaagtatta ccagcgcact tctataatgg cagacgtgtg ctcttccgat       60

c                                                                       61


<210>  5
<211>  49
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  linked with phosphorothiolate bonds

<400>  5
gaagtgcgct gtaagtatta cataatggca gacgtgtgct cttccgatc                   49


<210>  6
<211>  11
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer


<220>
<221>  misc_feature
<222>  (1)..(11)
<223>  linked with phosphorothiolate bonds

<400>  6
cagcgcactt c                                                            11


<210>  7
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SL(RCA-SLPrime)

<400>  7
gtccatcctc gtcctcctc                                                    19


<210>  8
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SL10Ta (RCA-SL10TaPrime)

<400>  8
agcagcgtcc tcctctgctg                                                   20


<210>  9
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16S-V3-F


<220>
<221>  misc_feature
<222>  (42)..(42)
<223>  n is a, c, g, or t

<400>  9
acactctttc cctacacgac gctcttccga tctcctacgg gnggcwgcag                  50


<210>  10
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  16S-V4-R

<400>  10
gtgactggag ttcagacgtg tgctcttccg atctgactac hvgggtatct aatcc            55


<210>  11
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the sequnce for exonuclease-resistant percentage test

<400>  11
ggcgaccacc gagatctaca cagrgttyga tymtggctca g                           41


<210>  12
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other end of a primer

<400>  12
gcagaagacg gcatacgaga trgytacctt gttacgactt                             40


<210>  13
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other end of a primer

<400>  13
gcagaagacg gcatacgaga tccaagtcga catcgtttac gg                          42


<210>  14
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other end of a primer

<400>  14
gcagaagacg gcatacgaga tccttcctcc ccgctgaaag ta                          42


<210>  15
<211>  41
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other end of a primer

<400>  15
gcagaagacg gcatacgaga tgcacatccg atggcaagag g                           41


<210>  16
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  the other end of a primer

<400>  16
gcagaagacg gcatacgaga tacattactc acccgtccgc cact                        44


<210>  17
<211>  64
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Looping oligo


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  linked with phosphorothiolate bonds

<400>  17
ggccagcagc agaggaggac gctgctggcc atatgaatga tacggcgacc accgagatct       60

acac                                                                    64


<210>  18
<211>  60
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  looping oligo


<220>
<221>  misc_feature
<222>  (1)..(6)
<223>  linked with phosphorothiolate bonds

<400>  18
gaagtgcgct gtaagtatta ccagcgcact tcatatcaag cagaagacgg catacgagat       60


