﻿                         序列表

<110>  浙江工业大学

<120>  一种多聚磷酸激酶突变体、工程菌及其应用

<160>  6

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  933
<212>  DNA
<213>  哈氏嗜纤维菌(Cytophaga hutchinsonii)

<400>  1
atggcaaccg attttagcaa actgagcaaa tatgttgaaa cgctgcgtgt gaaaccgaaa        60
cagagcattg atctgaaaaa ggattttgat accgattatg atcataaaat gctgacgaaa       120
gaagaaggtg aagaactgct gaatctgggt attagtaaac tgagcgaaat tcaggaaaaa       180
ctgtatgcat ctggcacaaa aagcgtgctg attgtttttc aggcaatgga tgcagcaggt       240
aaagatggta ccgttaaaca tattatgacg ggtctgaatc cgcagggtgt taaagtgacc       300
agctttaaag ttccgtccaa aattgaactg agtcatgatt atctgtggcg tcattatgtg       360
gcactgccgg caaccggcga aattggtatt tttaaccgta gccattatga aaatgtgctg       420
gttacccgtg tacatccgga atatctgctg agcgaacaga ccagcggtgt taccgcaatt       480
gaacaggtaa atcagaaatt ttgggataaa cgctttcagc agatcaataa ctttgaacag       540
catattagcg aaaacggtac cattgttctg aaattttttc tgcatgtttc caaaaaggaa       600
cagaaaaagc gttttattga acgtatcgaa ctggatacca aaaattggaa attttcaacc       660
ggtgatctga aagaacgtgc ccattggaaa gattatcgta atgcgtatga agatatgctg       720
gcaaatacct ctaccaaaca ggccccgtgg tttgttattc cggccgatga taaatggttt       780
acccgtctgc tgattgcaga aattatctgt accgaactgg aaaaactgaa tctgaccttt       840
ccgaccgtga gcctggaaca gaaagcggaa ctggaaaaag caaaagcaga actggttgca       900
gaaaaatcaa gcgatcatca tcatcaccac taa                                    933

<210>  2
<211>  311
<212>  PRT
<213>  哈氏嗜纤维菌(Cytophaga hutchinsonii)

<400>  2
Met Ala Thr Asp Phe Ser Lys Leu Ser Lys Tyr Val Glu Thr Leu Arg 
1               5                   10                  15      
Val Lys Pro Lys Gln Ser Ile Asp Leu Lys Lys Asp Phe Asp Thr Asp 
            20                  25                  30          
Tyr Asp His Lys Met Leu Thr Lys Glu Glu Gly Glu Glu Leu Leu Asn 
        35                  40                  45              
Leu Gly Ile Ser Lys Leu Ser Glu Ile Gln Glu Lys Leu Tyr Ala Ser 
    50                  55                  60                  
Gly Thr Lys Ser Val Leu Ile Val Phe Gln Ala Met Asp Ala Ala Gly 
65                  70                  75                  80  
Lys Asp Gly Thr Val Lys His Ile Met Thr Gly Leu Asn Pro Gln Gly 
                85                  90                  95      
Val Lys Val Thr Ser Phe Lys Val Pro Ser Lys Ile Glu Leu Ser His 
            100                 105                 110         
Asp Tyr Leu Trp Arg His Tyr Val Ala Leu Pro Ala Thr Gly Glu Ile 
        115                 120                 125             
Gly Ile Phe Asn Arg Ser His Tyr Glu Asn Val Leu Val Thr Arg Val 
    130                 135                 140                 
His Pro Glu Tyr Leu Leu Ser Glu Gln Thr Ser Gly Val Thr Ala Ile 
145                 150                 155                 160 
Glu Gln Val Asn Gln Lys Phe Trp Asp Lys Arg Phe Gln Gln Ile Asn 
                165                 170                 175     
Asn Phe Glu Gln His Ile Ser Glu Asn Gly Thr Ile Val Leu Lys Phe 
            180                 185                 190         
Phe Leu His Val Ser Lys Lys Glu Gln Lys Lys Arg Phe Ile Glu Arg 
        195                 200                 205             
Ile Glu Leu Asp Thr Lys Asn Trp Lys Phe Ser Thr Gly Asp Leu Lys 
    210                 215                 220                 
Glu Arg Ala His Trp Lys Asp Tyr Arg Asn Ala Tyr Glu Asp Met Leu 
225                 230                 235                 240 
Ala Asn Thr Ser Thr Lys Gln Ala Pro Trp Phe Val Ile Pro Ala Asp 
                245                 250                 255     
Asp Lys Trp Phe Thr Arg Leu Leu Ile Ala Glu Ile Ile Cys Thr Glu 
            260                 265                 270         
Leu Glu Lys Leu Asn Leu Thr Phe Pro Thr Val Ser Leu Glu Gln Lys 
        275                 280                 285             
Ala Glu Leu Glu Lys Ala Lys Ala Glu Leu Val Ala Glu Lys Ser Ser 
    290                 295                 300                 
Asp His His His His His His 
305                 310     

<210>  3
<211>  621
<212>  DNA
<213>  棕硬尾鸭(Oxyura jamaicensis)

<400>  3
atgaaataca tcatcggtat cggtggtgtt accaacggtg gcaaaaccac cctgacaaat        60
cgtctggtta aagcactgcc taactgttgt gtggttcacc aggacgattt ttttaaacct       120
caggatcaga ttgaagttgg tgaagatggc tttaaacaat gggacgttct ggactctctg       180
gatatggaag caatggttag caccgttcgt gcatggattg aaaatccggt taaatttgca       240
cgtagccacg gtgttaatgt tacaccgggc agcaaagaac cggcaagcaa agatattcat       300
attctggtta ttgagggatt tctgctgtat aattataaac cgctgattga cctgtttgat       360
attcgttatt atctggcagt cccttatgat gaatgtaaac gtcgtcgtag cacccgtaac       420
tataccgttc cggatccgcc gggtctgttc gatggccatg tttggccgat gtatctgaaa       480
catcgtaaag aaatggaaga caatggggtg gatgtggttt atctggatgg cctgaaaagc       540
cgcgatgaac tgtacaacca ggtctttgaa gatattcaga ataaactgct gaactgcctg       600
catcatcatc accaccatta a                                                 621

<210>  4
<211>  206
<212>  PRT
<213>  棕硬尾鸭(Oxyura jamaicensis)

<400>  4
Met Lys Tyr Ile Ile Gly Ile Gly Gly Val Thr Asn Gly Gly Lys Thr 
1               5                   10                  15      
Thr Leu Thr Asn Arg Leu Val Lys Ala Leu Pro Asn Cys Cys Val Val 
            20                  25                  30          
His Gln Asp Asp Phe Phe Lys Pro Gln Asp Gln Ile Glu Val Gly Glu 
        35                  40                  45              
Asp Gly Phe Lys Gln Trp Asp Val Leu Asp Ser Leu Asp Met Glu Ala 
    50                  55                  60                  
Met Val Ser Thr Val Arg Ala Trp Ile Glu Asn Pro Val Lys Phe Ala 
65                  70                  75                  80  
Arg Ser His Gly Val Asn Val Thr Pro Gly Ser Lys Glu Pro Ala Ser 
                85                  90                  95      
Lys Asp Ile His Ile Leu Val Ile Glu Gly Phe Leu Leu Tyr Asn Tyr 
            100                 105                 110         
Lys Pro Leu Ile Asp Leu Phe Asp Ile Arg Tyr Tyr Leu Ala Val Pro 
        115                 120                 125             
Tyr Asp Glu Cys Lys Arg Arg Arg Ser Thr Arg Asn Tyr Thr Val Pro 
    130                 135                 140                 
Asp Pro Pro Gly Leu Phe Asp Gly His Val Trp Pro Met Tyr Leu Lys 
145                 150                 155                 160 
His Arg Lys Glu Met Glu Asp Asn Gly Val Asp Val Val Tyr Leu Asp 
                165                 170                 175     
Gly Leu Lys Ser Arg Asp Glu Leu Tyr Asn Gln Val Phe Glu Asp Ile 
            180                 185                 190         
Gln Asn Lys Leu Leu Asn Cys Leu His His His His His His 
        195                 200                 205     

<210>  5
<211>  1302
<212>  DNA
<213>  酿酒酵母(Saccharomyces cerevisiae)

<400>  5
atgaccattg aaagcaccct ggcacgcgaa ctggaaagtc tgattctgcc ggcggatagc        60
attgtgaatg tggtggatca gtttcaggaa gaactgctga gccgcctgca gaccaacacc       120
attagcatgc tgccgcagtg cctggtgccg gataaacgca gccgctggaa tccggaagat       180
aaaattctga ccattgattt tggtggtacc cgtctgaaat ttgcgattat tagccttccg       240
cagattgtga ttgaatacaa cgatgcgttt gaactgacct ataacattgt ggattcaaat       300
ttctttaacc agatcattta taccatttgc acccgcctgg ccgccaatgg ttatatcaaa       360
aaaaaaaacg aaagctcaga agcgtcaaaa ttttttgtga gcgtgacctt tagctttccg       420
ctgaacccgg aaggcgaagt ggtggcgatg ggcaaaggtt ttgtgatgac cgataccctg       480
cagggcagca ccgtgaaaca gctgattcag agcagctttc atcgcattat tagcgagaat       540
attgaagagt ttttttgcac catgaatgtg tgtcatgtga ttaatgatgc cattgccgtg       600
agcctgacca gcaaatttat ttgtgaaaac gatagcatca gcctgattat tggcaccggt       660
accaatgcgt gctttgaagt gccgtatggc tatctgccgc cgtttaaacg cgatgcgctg       720
cgcgaaaccc tgccgagcag ctacaacaaa gaaaccctga attttaaaca tgtgctgatc       780
aacagcgaaa tcggctttat tggcaaaaat gtcattgcgc tgcagccgtt tgatattcac       840
ggcgcaatta gctatgaaat gccgctggaa tgcgtgacca gcggcaaatg gctgccgctg       900
agcctgaaaa acattctgct gcaatataat attattccga aaaattttcc ggttgaattt       960
aatggagaac tggtgtgcca gctggcggaa gattgcacca atgcgtggtt tgaaaatgaa      1020
cattatgccc tgatttgcca gattgcgcgc ctgttgatta aacgcgcagc gttctacgtg      1080
gcggccattg tgcaggcgat tgatattatc accggctgca aaaattataa ttttattcac      1140
attggctatg tgggctcatt tctgcataac agcaactttt accgtgaaca gattaaatat      1200
tatagcagca ttcacattaa actgcagttc ctgaatcact caaatctgct gggtgcggcc      1260
attgccacct acctgaataa atcagataac caggtgcagt aa                         1302

<210>  6
<211>  433
<212>  PRT
<213>  酿酒酵母(Saccharomyces cerevisiae)

<400>  6
Met Thr Ile Glu Ser Thr Leu Ala Arg Glu Leu Glu Ser Leu Ile Leu 
1               5                   10                  15      
Pro Ala Asp Ser Ile Val Asn Val Val Asp Gln Phe Gln Glu Glu Leu 
            20                  25                  30          
Leu Ser Arg Leu Gln Thr Asn Thr Ile Ser Met Leu Pro Gln Cys Leu 
        35                  40                  45              
Val Pro Asp Lys Arg Ser Arg Trp Asn Pro Glu Asp Lys Ile Leu Thr 
    50                  55                  60                  
Ile Asp Phe Gly Gly Thr Arg Leu Lys Phe Ala Ile Ile Ser Leu Pro 
65                  70                  75                  80  
Gln Ile Val Ile Glu Tyr Asn Asp Ala Phe Glu Leu Thr Tyr Asn Ile 
                85                  90                  95      
Val Asp Ser Asn Phe Phe Asn Gln Ile Ile Tyr Thr Ile Cys Thr Arg 
            100                 105                 110         
Leu Ala Ala Asn Gly Tyr Ile Lys Lys Lys Asn Glu Ser Ser Glu Ala 
        115                 120                 125             
Ser Lys Phe Phe Val Ser Val Thr Phe Ser Phe Pro Leu Asn Pro Glu 
    130                 135                 140                 
Gly Glu Val Val Ala Met Gly Lys Gly Phe Val Met Thr Asp Thr Leu 
145                 150                 155                 160 
Gln Gly Ser Thr Val Lys Gln Leu Ile Gln Ser Ser Phe His Arg Ile 
                165                 170                 175     
Ile Ser Glu Asn Ile Glu Glu Phe Phe Cys Thr Met Asn Val Cys His 
            180                 185                 190         
Val Ile Asn Asp Ala Ile Ala Val Ser Leu Thr Ser Lys Phe Ile Cys 
        195                 200                 205             
Glu Asn Asp Ser Ile Ser Leu Ile Ile Gly Thr Gly Thr Asn Ala Cys 
    210                 215                 220                 
Phe Glu Val Pro Tyr Gly Tyr Leu Pro Pro Phe Lys Arg Asp Ala Leu 
225                 230                 235                 240 
Arg Glu Thr Leu Pro Ser Ser Tyr Asn Lys Glu Thr Leu Asn Phe Lys 
                245                 250                 255     
His Val Leu Ile Asn Ser Glu Ile Gly Phe Ile Gly Lys Asn Val Ile 
            260                 265                 270         
Ala Leu Gln Pro Phe Asp Ile His Gly Ala Ile Ser Tyr Glu Met Pro 
        275                 280                 285             
Leu Glu Cys Val Thr Ser Gly Lys Trp Leu Pro Leu Ser Leu Lys Asn 
    290                 295                 300                 
Ile Leu Leu Gln Tyr Asn Ile Ile Pro Lys Asn Phe Pro Val Glu Phe 
305                 310                 315                 320 
Asn Gly Glu Leu Val Cys Gln Leu Ala Glu Asp Cys Thr Asn Ala Trp 
                325                 330                 335     
Phe Glu Asn Glu His Tyr Ala Leu Ile Cys Gln Ile Ala Arg Leu Leu 
            340                 345                 350         
Ile Lys Arg Ala Ala Phe Tyr Val Ala Ala Ile Val Gln Ala Ile Asp 
        355                 360                 365             
Ile Ile Thr Gly Cys Lys Asn Tyr Asn Phe Ile His Ile Gly Tyr Val 
    370                 375                 380                 
Gly Ser Phe Leu His Asn Ser Asn Phe Tyr Arg Glu Gln Ile Lys Tyr 
385                 390                 395                 400 
Tyr Ser Ser Ile His Ile Lys Leu Gln Phe Leu Asn His Ser Asn Leu 
                405                 410                 415     
Leu Gly Ala Ala Ile Ala Thr Tyr Leu Asn Lys Ser Asp Asn Gln Val 
            420                 425                 430         
Gln 
    

