                         SEQUENCE LISTING

<110>  THE GOVERNING COUNCIL OF THE UNIVERSIY OF TORONTO ET AL.
 
<120>  NEUTRALIZATION ASSAY TO RAPIDLY ASSESS NEUTRALIZATION ACTIVITY OF
        ANTI-SARS-COV-2 ANTIBODIES

<130>  0180354.0075

<140>  PCT/CA2022/050924
<141>  2022-06-09

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: DELTA-11S nucleotide sequence

<400>  1
atgggccatc atcatcatca tcatcatcat atggttttta cacttgaaga ttttgtgggt       60

gattgggaac aaactgctgc atataattta gatcaagttt tagaacaggg tggagtgagt      120

tctcttttac aaaatcttgc agtctcagtt acaccaatac aaagaatagt tagaagtgga      180

gaaaatgcat taaagatcga tatacatgta ataattcctt atgagggact atcagcagac      240

caaatggcac aaattgagga agtattcaaa gttgtatatc cagtagacga tcatcacttt      300

aaagtaatat taccttatgg aactttagta attgatggtg taacaccaaa tatgttaaat      360

tattttggta gaccatacga agggattgca gtttttgatg gaaagaaaat aaccgtaaca      420

ggcactttgt ggaatggaaa taaaataata gatgaaaggt taattacacc tgattaa         477


<210>  2
<211>  158
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: DELTA-11S amino acid sequence

<400>  2

Met Gly His His His His His His His His Met Val Phe Thr Leu Glu 
1               5                   10                  15      


Asp Phe Val Gly Asp Trp Glu Gln Thr Ala Ala Tyr Asn Leu Asp Gln 
            20                  25                  30          


Val Leu Glu Gln Gly Gly Val Ser Ser Leu Leu Gln Asn Leu Ala Val 
        35                  40                  45              


Ser Val Thr Pro Ile Gln Arg Ile Val Arg Ser Gly Glu Asn Ala Leu 
    50                  55                  60                  


Lys Ile Asp Ile His Val Ile Ile Pro Tyr Glu Gly Leu Ser Ala Asp 
65                  70                  75                  80  


Gln Met Ala Gln Ile Glu Glu Val Phe Lys Val Val Tyr Pro Val Asp 
                85                  90                  95      


Asp His His Phe Lys Val Ile Leu Pro Tyr Gly Thr Leu Val Ile Asp 
            100                 105                 110         


Gly Val Thr Pro Asn Met Leu Asn Tyr Phe Gly Arg Pro Tyr Glu Gly 
        115                 120                 125             


Ile Ala Val Phe Asp Gly Lys Lys Ile Thr Val Thr Gly Thr Leu Trp 
    130                 135                 140                 


Asn Gly Asn Lys Ile Ile Asp Glu Arg Leu Ile Thr Pro Asp 
145                 150                 155             


<210>  3
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9 nucleotide sequence

<400>  3
ggctccatgc tgttccgagt aaccatcaac agt                                    33


<210>  4
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9 amino acid sequence

<400>  4

Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser 
1               5                   10      


<210>  5
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: BETA10 nucleotide sequence

<400>  5
gtgagcggct ggcggctgtt caagaagatt agc                                    33


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: BETA10 amino acid sequence

<400>  6

Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser 
1               5                   10      


<210>  7
<211>  867
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-(S)RBD

<400>  7
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg ggggctccat gctgttccga      120

gtaaccatca acagtggtgg cggaggatct ggaggtggat ccagggtgca gccaaccgag      180

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      240

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      300

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      360

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      420

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      480

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      540

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      600

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      660

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      720

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      780

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggaagc      840

catcatcacc accatcatca ccattga                                          867


<210>  8
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-(S)RBD

<400>  8

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
    50                  55                  60                  


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
65                  70                  75                  80  


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                85                  90                  95      


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
            100                 105                 110         


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
        115                 120                 125             


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
    130                 135                 140                 


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
145                 150                 155                 160 


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
                165                 170                 175     


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
            180                 185                 190         


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
        195                 200                 205             


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
    210                 215                 220                 


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
225                 230                 235                 240 


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
                245                 250                 255     


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
            260                 265                 270         


Lys Cys Val Asn Phe Gly Gly Ser His His His His His His His His 
        275                 280                 285             


<210>  9
<211>  807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: (S)RBD-Beta9

<400>  9
atgttcgtct tcctggtcct gctgcctctg gtctcctcac agagggtgca gccaaccgag       60

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      120

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      180

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      240

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      300

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      360

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      420

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      480

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      540

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      600

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      660

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggagga      720

ggatctggag gtggatccgg ctccatgctg ttccgagtaa ccatcaacag tggcggaagc      780

catcatcacc accatcatca ccattga                                          807


<210>  10
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: (S)RBD-Beta9

<400>  10

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Arg Val 
1               5                   10                  15      


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
    210                 215                 220                 


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Ser Gly Ser Met Leu Phe Arg Val Thr Ile Asn 
                245                 250                 255     


Ser Gly Gly Ser His His His His His His His His 
            260                 265             


<210>  11
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-(S)RBD-Beta9

<400>  11
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg ggggctccat gctgttccga      120

gtaaccatca acagtggtgg cggaggatct ggaggtggat ccagggtgca gccaaccgag      180

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      240

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      300

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      360

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      420

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      480

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      540

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      600

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      660

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      720

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      780

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggagga      840

ggatctggag gtggatccgg ctccatgctg ttccgagtaa ccatcaacag tggcggaagc      900

catcatcacc accatcatca ccattga                                          927


<210>  12
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-(S)RBD-Beta9

<400>  12

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
    50                  55                  60                  


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
65                  70                  75                  80  


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                85                  90                  95      


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
            100                 105                 110         


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
        115                 120                 125             


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
    130                 135                 140                 


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
145                 150                 155                 160 


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
                165                 170                 175     


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
            180                 185                 190         


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
        195                 200                 205             


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
    210                 215                 220                 


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
225                 230                 235                 240 


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
                245                 250                 255     


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
            260                 265                 270         


Lys Cys Val Asn Phe Gly Gly Gly Gly Ser Gly Gly Gly Ser Gly Ser 
        275                 280                 285             


Met Leu Phe Arg Val Thr Ile Asn Ser Gly Gly Ser His His His His 
    290                 295                 300                 


His His His His 
305             


<210>  13
<211>  867
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-(S)RBD

<400>  13
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg gagtgagcgg ctggcggctg      120

ttcaagaaga ttagcggtgg cggaggatct ggaggtggat ccagggtgca gccaaccgag      180

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      240

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      300

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      360

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      420

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      480

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      540

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      600

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      660

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      720

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      780

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggaagc      840

catcatcacc accatcatca ccattga                                          867


<210>  14
<211>  288
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-(S)RBD

<400>  14

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
    50                  55                  60                  


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
65                  70                  75                  80  


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                85                  90                  95      


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
            100                 105                 110         


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
        115                 120                 125             


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
    130                 135                 140                 


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
145                 150                 155                 160 


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
                165                 170                 175     


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
            180                 185                 190         


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
        195                 200                 205             


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
    210                 215                 220                 


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
225                 230                 235                 240 


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
                245                 250                 255     


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
            260                 265                 270         


Lys Cys Val Asn Phe Gly Gly Ser His His His His His His His His 
        275                 280                 285             


<210>  15
<211>  807
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: (S)RBD-Beta10

<400>  15
atgttcgtct tcctggtcct gctgcctctg gtctcctcac agagggtgca gccaaccgag       60

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      120

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      180

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      240

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      300

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      360

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      420

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      480

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      540

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      600

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      660

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggagga      720

ggatctggag gtggatccgt gagcggctgg cggctgttca agaagattag cggcggaagc      780

catcatcacc accatcatca ccattga                                          807


<210>  16
<211>  268
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: (S)RBD-Beta10

<400>  16

Met Phe Val Phe Leu Val Leu Leu Pro Leu Val Ser Ser Gln Arg Val 
1               5                   10                  15      


Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu Cys 
            20                  25                  30          


Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr Ala 
        35                  40                  45              


Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val Leu 
    50                  55                  60                  


Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser Pro 
65                  70                  75                  80  


Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser Phe 
                85                  90                  95      


Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr Gly 
            100                 105                 110         


Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly Cys 
        115                 120                 125             


Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly Asn 
    130                 135                 140                 


Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro Phe 
145                 150                 155                 160 


Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro Cys 
                165                 170                 175     


Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr Gly 
            180                 185                 190         


Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val Val 
        195                 200                 205             


Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro Lys 
    210                 215                 220                 


Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Gly Gly Gly 
225                 230                 235                 240 


Gly Ser Gly Gly Gly Ser Val Ser Gly Trp Arg Leu Phe Lys Lys Ile 
                245                 250                 255     


Ser Gly Gly Ser His His His His His His His His 
            260                 265             


<210>  17
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-(S)RBD-Beta10

<400>  17
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg gagtgagcgg ctggcggctg      120

ttcaagaaga ttagcggtgg cggaggatct ggaggtggat ccagggtgca gccaaccgag      180

tctatcgtgc gctttcctaa tatcacaaac ctgtgcccat ttggcgaggt gttcaacgca      240

acccgcttcg ccagcgtgta cgcctggaat aggaagcgga tcagcaactg cgtggccgac      300

tatagcgtgc tgtacaactc cgcctctttc agcaccttta agtgctatgg cgtgtccccc      360

acaaagctga atgacctgtg ctttaccaac gtctacgccg attctttcgt gatcaggggc      420

gacgaggtgc gccagatcgc ccccggccag acaggcaaga tcgcagacta caattataag      480

ctgccagacg atttcaccgg ctgcgtgatc gcctggaaca gcaacaatct ggattccaaa      540

gtgggcggca actacaatta tctgtaccgg ctgtttagaa agagcaatct gaagcccttc      600

gagagggaca tctctacaga aatctaccag gccggcagca ccccttgcaa tggcgtggag      660

ggctttaact gttatttccc actccagtcc tacggcttcc agcccacaaa cggcgtgggc      720

tatcagcctt accgcgtggt ggtgctgagc tttgagctgc tgcacgcccc agcaacagtg      780

tgcggcccca agaagtccac caatctggtg aagaacaagt gcgtgaactt cggcggagga      840

ggatctggag gtggatccgt gagcggctgg cggctgttca agaagattag cggcggaagc      900

catcatcacc accatcatca ccattga                                          927


<210>  18
<211>  308
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-(S)RBD-Beta10

<400>  18

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Arg Val Gln Pro Thr Glu Ser Ile Val Arg 
    50                  55                  60                  


Phe Pro Asn Ile Thr Asn Leu Cys Pro Phe Gly Glu Val Phe Asn Ala 
65                  70                  75                  80  


Thr Arg Phe Ala Ser Val Tyr Ala Trp Asn Arg Lys Arg Ile Ser Asn 
                85                  90                  95      


Cys Val Ala Asp Tyr Ser Val Leu Tyr Asn Ser Ala Ser Phe Ser Thr 
            100                 105                 110         


Phe Lys Cys Tyr Gly Val Ser Pro Thr Lys Leu Asn Asp Leu Cys Phe 
        115                 120                 125             


Thr Asn Val Tyr Ala Asp Ser Phe Val Ile Arg Gly Asp Glu Val Arg 
    130                 135                 140                 


Gln Ile Ala Pro Gly Gln Thr Gly Lys Ile Ala Asp Tyr Asn Tyr Lys 
145                 150                 155                 160 


Leu Pro Asp Asp Phe Thr Gly Cys Val Ile Ala Trp Asn Ser Asn Asn 
                165                 170                 175     


Leu Asp Ser Lys Val Gly Gly Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe 
            180                 185                 190         


Arg Lys Ser Asn Leu Lys Pro Phe Glu Arg Asp Ile Ser Thr Glu Ile 
        195                 200                 205             


Tyr Gln Ala Gly Ser Thr Pro Cys Asn Gly Val Glu Gly Phe Asn Cys 
    210                 215                 220                 


Tyr Phe Pro Leu Gln Ser Tyr Gly Phe Gln Pro Thr Asn Gly Val Gly 
225                 230                 235                 240 


Tyr Gln Pro Tyr Arg Val Val Val Leu Ser Phe Glu Leu Leu His Ala 
                245                 250                 255     


Pro Ala Thr Val Cys Gly Pro Lys Lys Ser Thr Asn Leu Val Lys Asn 
            260                 265                 270         


Lys Cys Val Asn Phe Gly Gly Gly Gly Ser Gly Gly Gly Ser Val Ser 
        275                 280                 285             


Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Gly Ser His His His His 
    290                 295                 300                 


His His His His 
305             


<210>  19
<211>  3915
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-spike (18 G/S linker)

<400>  19
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg gagtgagcgg ctggcggctg      120

ttcaagaaga ttagtggtgg cggaggatct ggaggtggat ccggtggcgg aggatctgga      180

ggtggatcct gcgtcaatct gacaactcgg actcagctgc cacctgctta tactaatagc      240

ttcaccagag gcgtgtacta tcctgacaag gtgtttagaa gctccgtgct gcactctaca      300

caggatctgt ttctgccatt ctttagcaac gtgacctggt tccacgccat ccacgtgagc      360

ggcaccaatg gcacaaagcg gttcgacaat cccgtgctgc cttttaacga tggcgtgtac      420

ttcgcctcta ccgagaagag caacatcatc agaggctgga tctttggcac cacactggac      480

tccaagacac agtctctgct gatcgtgaac aatgccacca acgtggtcat caaggtgtgc      540

gagttccagt tttgtaatga tcccttcctg ggcgtgtact atcacaagaa caataagagc      600

tggatggagt ccgagtttag agtgtattct agcgccaaca actgcacatt tgagtacgtg      660

agccagcctt tcctgatgga cctggagggc aagcagggca atttcaagaa cctgagggag      720

ttcgtgttta agaatatcga cggctacttc aaaatctact ctaagcacac ccccatcaac      780

ctggtgcgcg acctgcctca gggcttcagc gccctggagc ccctggtgga tctgcctatc      840

ggcatcaaca tcacccggtt tcagacactg ctggccctgc acagaagcta cctgacaccc      900

ggcgactcct ctagcggatg gaccgccggc gctgccgcct actatgtggg ctacctccag      960

ccccggacct tcctgctgaa gtacaacgag aatggcacca tcacagacgc agtggattgc     1020

gccctggacc ccctgagcga gacaaagtgt acactgaagt cctttaccgt ggagaagggc     1080

atctatcaga catccaattt cagggtgcag ccaaccgagt ctatcgtgcg ctttcctaat     1140

atcacaaacc tgtgcccatt tggcgaggtg ttcaacgcaa cccgcttcgc cagcgtgtac     1200

gcctggaata ggaagcggat cagcaactgc gtggccgact atagcgtgct gtacaactcc     1260

gcctctttca gcacctttaa gtgctatggc gtgtccccca caaagctgaa tgacctgtgc     1320

tttaccaacg tctacgccga ttctttcgtg atcaggggcg acgaggtgcg ccagatcgcc     1380

cccggccaga caggcaagat cgcagactac aattataagc tgccagacga tttcaccggc     1440

tgcgtgatcg cctggaacag caacaatctg gattccaaag tgggcggcaa ctacaattat     1500

ctgtaccggc tgtttagaaa gagcaatctg aagcccttcg agagggacat ctctacagaa     1560

atctaccagg ccggcagcac cccttgcaat ggcgtggagg gctttaactg ttatttccca     1620

ctccagtcct acggcttcca gcccacaaac ggcgtgggct atcagcctta ccgcgtggtg     1680

gtgctgagct ttgagctgct gcacgcccca gcaacagtgt gcggccccaa gaagtccacc     1740

aatctggtga agaacaagtg cgtgaacttc aacttcaacg gcctgaccgg cacaggcgtg     1800

ctgaccgagt ccaacaagaa gttcctgcca tttcagcagt tcggcaggga catcgcagat     1860

accacagacg ccgtgcgcga cccacagacc ctggagatcc tggacatcac accctgctct     1920

ttcggcggcg tgagcgtgat cacacccggc accaatacaa gcaaccaggt ggccgtgctg     1980

tatcaggacg tgaattgtac cgaggtgccc gtggctatcc acgccgatca gctgacccca     2040

acatggcggg tgtacagcac cggctccaac gtcttccaga caagagccgg atgcctgatc     2100

ggagcagagc acgtgaacaa ttcctatgag tgcgacatcc caatcggcgc cggcatctgt     2160

gcctcttacc agacccagac aaactctccc ggaagcgcca gcagcgtggc ctcccagtct     2220

atcatcgcct ataccatgtc cctgggcgcc gagaacagcg tggcctactc taacaatagc     2280

atcgccatcc caaccaactt cacaatctct gtgaccacag agatcctgcc cgtgtccatg     2340

accaagacat ctgtggactg cacaatgtat atctgtggcg attctaccga gtgcagcaac     2400

ctgctgctcc agtacggcag cttttgtacc cagctgaata gagccctgac aggcatcgcc     2460

gtggagcagg ataagaacac acaggaggtg ttcgcccagg tgaagcaaat ctacaagacc     2520

ccccctatca aggactttgg cggcttcaat ttttcccaga tcctgcctga tccatccaag     2580

ccttctaagc ggagctttat cgaggacctg ctgttcaaca aggtgaccct ggccgatgcc     2640

ggcttcatca agcagtatgg cgattgcctg ggcgacatcg cagccaggga cctgatctgc     2700

gcccagaagt ttaatggcct gaccgtgctg ccacccctgc tgacagatga gatgatcgca     2760

cagtacacaa gcgccctgct ggccggcacc atcacatccg gatggacctt cggcgcagga     2820

gccgccctcc agatcccctt tgccatgcag atggcctata ggttcaacgg catcggcgtg     2880

acccagaatg tgctgtacga gaaccagaag ctgatcgcca atcagtttaa ctccgccatc     2940

ggcaagatcc aggacagcct gtcctctaca gccagcgccc tgggcaagct ccaggatgtg     3000

gtgaatcaga acgcccaggc cctgaatacc ctggtgaagc agctgagcag caacttcggc     3060

gccatctcta gcgtgctgaa tgacatcctg agccggctgg accctcctga ggcagaggtg     3120

cagatcgacc ggctgatcac cggccggctc cagagcctcc agacctatgt gacacagcag     3180

ctgatcaggg ccgccgagat cagggccagc gccaatctgg cagcaaccaa gatgtccgag     3240

tgcgtgctgg gccagtctaa gagagtggac ttttgtggca agggctatca cctgatgtcc     3300

ttccctcagt ctgccccaca cggcgtggtg tttctgcacg tgacctacgt gcccgcccag     3360

gagaagaact tcaccacagc ccctgccatc tgccacgatg gcaaggccca ctttccaagg     3420

gagggcgtgt tcgtgtccaa cggcacccac tggtttgtga cacagcgcaa tttctacgag     3480

ccccagatca tcaccacaga caacaccttc gtgagcggca actgtgacgt ggtcatcggc     3540

atcgtgaaca ataccgtgta tgatccactc cagcccgagc tggacagctt taaggaggag     3600

ctggataagt atttcaagaa tcacacctcc cctgacgtgg atctgggcga catcagcggc     3660

atcaatgcct ccgtggtgaa catccagaag gagatcgacc gcctgaacga ggtggctaag     3720

aatctgaacg agagcctgat cgacctccag gagctgggca agtatgagca gtacatcaag     3780

tggcccggag gcagcggagg ctacatcccc gaggcccctc gcgatggaca ggcttacgtg     3840

cgcaaggacg gcgagtgggt gctgctgagc accttcctgg gcggaagcca tcatcaccac     3900

catcatcacc attga                                                      3915


<210>  20
<211>  1304
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-spike (18 G/S linker)

<400>  20

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Cys 
    50                  55                  60                  


Val Asn Leu Thr Thr Arg Thr Gln Leu Pro Pro Ala Tyr Thr Asn Ser 
65                  70                  75                  80  


Phe Thr Arg Gly Val Tyr Tyr Pro Asp Lys Val Phe Arg Ser Ser Val 
                85                  90                  95      


Leu His Ser Thr Gln Asp Leu Phe Leu Pro Phe Phe Ser Asn Val Thr 
            100                 105                 110         


Trp Phe His Ala Ile His Val Ser Gly Thr Asn Gly Thr Lys Arg Phe 
        115                 120                 125             


Asp Asn Pro Val Leu Pro Phe Asn Asp Gly Val Tyr Phe Ala Ser Thr 
    130                 135                 140                 


Glu Lys Ser Asn Ile Ile Arg Gly Trp Ile Phe Gly Thr Thr Leu Asp 
145                 150                 155                 160 


Ser Lys Thr Gln Ser Leu Leu Ile Val Asn Asn Ala Thr Asn Val Val 
                165                 170                 175     


Ile Lys Val Cys Glu Phe Gln Phe Cys Asn Asp Pro Phe Leu Gly Val 
            180                 185                 190         


Tyr Tyr His Lys Asn Asn Lys Ser Trp Met Glu Ser Glu Phe Arg Val 
        195                 200                 205             


Tyr Ser Ser Ala Asn Asn Cys Thr Phe Glu Tyr Val Ser Gln Pro Phe 
    210                 215                 220                 


Leu Met Asp Leu Glu Gly Lys Gln Gly Asn Phe Lys Asn Leu Arg Glu 
225                 230                 235                 240 


Phe Val Phe Lys Asn Ile Asp Gly Tyr Phe Lys Ile Tyr Ser Lys His 
                245                 250                 255     


Thr Pro Ile Asn Leu Val Arg Asp Leu Pro Gln Gly Phe Ser Ala Leu 
            260                 265                 270         


Glu Pro Leu Val Asp Leu Pro Ile Gly Ile Asn Ile Thr Arg Phe Gln 
        275                 280                 285             


Thr Leu Leu Ala Leu His Arg Ser Tyr Leu Thr Pro Gly Asp Ser Ser 
    290                 295                 300                 


Ser Gly Trp Thr Ala Gly Ala Ala Ala Tyr Tyr Val Gly Tyr Leu Gln 
305                 310                 315                 320 


Pro Arg Thr Phe Leu Leu Lys Tyr Asn Glu Asn Gly Thr Ile Thr Asp 
                325                 330                 335     


Ala Val Asp Cys Ala Leu Asp Pro Leu Ser Glu Thr Lys Cys Thr Leu 
            340                 345                 350         


Lys Ser Phe Thr Val Glu Lys Gly Ile Tyr Gln Thr Ser Asn Phe Arg 
        355                 360                 365             


Val Gln Pro Thr Glu Ser Ile Val Arg Phe Pro Asn Ile Thr Asn Leu 
    370                 375                 380                 


Cys Pro Phe Gly Glu Val Phe Asn Ala Thr Arg Phe Ala Ser Val Tyr 
385                 390                 395                 400 


Ala Trp Asn Arg Lys Arg Ile Ser Asn Cys Val Ala Asp Tyr Ser Val 
                405                 410                 415     


Leu Tyr Asn Ser Ala Ser Phe Ser Thr Phe Lys Cys Tyr Gly Val Ser 
            420                 425                 430         


Pro Thr Lys Leu Asn Asp Leu Cys Phe Thr Asn Val Tyr Ala Asp Ser 
        435                 440                 445             


Phe Val Ile Arg Gly Asp Glu Val Arg Gln Ile Ala Pro Gly Gln Thr 
    450                 455                 460                 


Gly Lys Ile Ala Asp Tyr Asn Tyr Lys Leu Pro Asp Asp Phe Thr Gly 
465                 470                 475                 480 


Cys Val Ile Ala Trp Asn Ser Asn Asn Leu Asp Ser Lys Val Gly Gly 
                485                 490                 495     


Asn Tyr Asn Tyr Leu Tyr Arg Leu Phe Arg Lys Ser Asn Leu Lys Pro 
            500                 505                 510         


Phe Glu Arg Asp Ile Ser Thr Glu Ile Tyr Gln Ala Gly Ser Thr Pro 
        515                 520                 525             


Cys Asn Gly Val Glu Gly Phe Asn Cys Tyr Phe Pro Leu Gln Ser Tyr 
    530                 535                 540                 


Gly Phe Gln Pro Thr Asn Gly Val Gly Tyr Gln Pro Tyr Arg Val Val 
545                 550                 555                 560 


Val Leu Ser Phe Glu Leu Leu His Ala Pro Ala Thr Val Cys Gly Pro 
                565                 570                 575     


Lys Lys Ser Thr Asn Leu Val Lys Asn Lys Cys Val Asn Phe Asn Phe 
            580                 585                 590         


Asn Gly Leu Thr Gly Thr Gly Val Leu Thr Glu Ser Asn Lys Lys Phe 
        595                 600                 605             


Leu Pro Phe Gln Gln Phe Gly Arg Asp Ile Ala Asp Thr Thr Asp Ala 
    610                 615                 620                 


Val Arg Asp Pro Gln Thr Leu Glu Ile Leu Asp Ile Thr Pro Cys Ser 
625                 630                 635                 640 


Phe Gly Gly Val Ser Val Ile Thr Pro Gly Thr Asn Thr Ser Asn Gln 
                645                 650                 655     


Val Ala Val Leu Tyr Gln Asp Val Asn Cys Thr Glu Val Pro Val Ala 
            660                 665                 670         


Ile His Ala Asp Gln Leu Thr Pro Thr Trp Arg Val Tyr Ser Thr Gly 
        675                 680                 685             


Ser Asn Val Phe Gln Thr Arg Ala Gly Cys Leu Ile Gly Ala Glu His 
    690                 695                 700                 


Val Asn Asn Ser Tyr Glu Cys Asp Ile Pro Ile Gly Ala Gly Ile Cys 
705                 710                 715                 720 


Ala Ser Tyr Gln Thr Gln Thr Asn Ser Pro Gly Ser Ala Ser Ser Val 
                725                 730                 735     


Ala Ser Gln Ser Ile Ile Ala Tyr Thr Met Ser Leu Gly Ala Glu Asn 
            740                 745                 750         


Ser Val Ala Tyr Ser Asn Asn Ser Ile Ala Ile Pro Thr Asn Phe Thr 
        755                 760                 765             


Ile Ser Val Thr Thr Glu Ile Leu Pro Val Ser Met Thr Lys Thr Ser 
    770                 775                 780                 


Val Asp Cys Thr Met Tyr Ile Cys Gly Asp Ser Thr Glu Cys Ser Asn 
785                 790                 795                 800 


Leu Leu Leu Gln Tyr Gly Ser Phe Cys Thr Gln Leu Asn Arg Ala Leu 
                805                 810                 815     


Thr Gly Ile Ala Val Glu Gln Asp Lys Asn Thr Gln Glu Val Phe Ala 
            820                 825                 830         


Gln Val Lys Gln Ile Tyr Lys Thr Pro Pro Ile Lys Asp Phe Gly Gly 
        835                 840                 845             


Phe Asn Phe Ser Gln Ile Leu Pro Asp Pro Ser Lys Pro Ser Lys Arg 
    850                 855                 860                 


Ser Phe Ile Glu Asp Leu Leu Phe Asn Lys Val Thr Leu Ala Asp Ala 
865                 870                 875                 880 


Gly Phe Ile Lys Gln Tyr Gly Asp Cys Leu Gly Asp Ile Ala Ala Arg 
                885                 890                 895     


Asp Leu Ile Cys Ala Gln Lys Phe Asn Gly Leu Thr Val Leu Pro Pro 
            900                 905                 910         


Leu Leu Thr Asp Glu Met Ile Ala Gln Tyr Thr Ser Ala Leu Leu Ala 
        915                 920                 925             


Gly Thr Ile Thr Ser Gly Trp Thr Phe Gly Ala Gly Ala Ala Leu Gln 
    930                 935                 940                 


Ile Pro Phe Ala Met Gln Met Ala Tyr Arg Phe Asn Gly Ile Gly Val 
945                 950                 955                 960 


Thr Gln Asn Val Leu Tyr Glu Asn Gln Lys Leu Ile Ala Asn Gln Phe 
                965                 970                 975     


Asn Ser Ala Ile Gly Lys Ile Gln Asp Ser Leu Ser Ser Thr Ala Ser 
            980                 985                 990         


Ala Leu Gly Lys Leu Gln Asp Val  Val Asn Gln Asn Ala  Gln Ala Leu 
        995                 1000                 1005             


Asn Thr  Leu Val Lys Gln Leu  Ser Ser Asn Phe Gly  Ala Ile Ser 
    1010                 1015                 1020             


Ser Val  Leu Asn Asp Ile Leu  Ser Arg Leu Asp Pro  Pro Glu Ala 
    1025                 1030                 1035             


Glu Val  Gln Ile Asp Arg Leu  Ile Thr Gly Arg Leu  Gln Ser Leu 
    1040                 1045                 1050             


Gln Thr  Tyr Val Thr Gln Gln  Leu Ile Arg Ala Ala  Glu Ile Arg 
    1055                 1060                 1065             


Ala Ser  Ala Asn Leu Ala Ala  Thr Lys Met Ser Glu  Cys Val Leu 
    1070                 1075                 1080             


Gly Gln  Ser Lys Arg Val Asp  Phe Cys Gly Lys Gly  Tyr His Leu 
    1085                 1090                 1095             


Met Ser  Phe Pro Gln Ser Ala  Pro His Gly Val Val  Phe Leu His 
    1100                 1105                 1110             


Val Thr  Tyr Val Pro Ala Gln  Glu Lys Asn Phe Thr  Thr Ala Pro 
    1115                 1120                 1125             


Ala Ile  Cys His Asp Gly Lys  Ala His Phe Pro Arg  Glu Gly Val 
    1130                 1135                 1140             


Phe Val  Ser Asn Gly Thr His  Trp Phe Val Thr Gln  Arg Asn Phe 
    1145                 1150                 1155             


Tyr Glu  Pro Gln Ile Ile Thr  Thr Asp Asn Thr Phe  Val Ser Gly 
    1160                 1165                 1170             


Asn Cys  Asp Val Val Ile Gly  Ile Val Asn Asn Thr  Val Tyr Asp 
    1175                 1180                 1185             


Pro Leu  Gln Pro Glu Leu Asp  Ser Phe Lys Glu Glu  Leu Asp Lys 
    1190                 1195                 1200             


Tyr Phe  Lys Asn His Thr Ser  Pro Asp Val Asp Leu  Gly Asp Ile 
    1205                 1210                 1215             


Ser Gly  Ile Asn Ala Ser Val  Val Asn Ile Gln Lys  Glu Ile Asp 
    1220                 1225                 1230             


Arg Leu  Asn Glu Val Ala Lys  Asn Leu Asn Glu Ser  Leu Ile Asp 
    1235                 1240                 1245             


Leu Gln  Glu Leu Gly Lys Tyr  Glu Gln Tyr Ile Lys  Trp Pro Gly 
    1250                 1255                 1260             


Gly Ser  Gly Gly Tyr Ile Pro  Glu Ala Pro Arg Asp  Gly Gln Ala 
    1265                 1270                 1275             


Tyr Val  Arg Lys Asp Gly Glu  Trp Val Leu Leu Ser  Thr Phe Leu 
    1280                 1285                 1290             


Gly Gly  Ser His His His His  His His His His 
    1295                 1300                 


<210>  21
<211>  1995
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-ACE2 (9 G/S linker)

<400>  21
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg ggggctccat gctgttccga      120

gtaaccatca acagtggtgg cggaggatct ggaggtggat ccgcagcaca gagcaccatt      180

gaagaacagg caaaaacctt tctggacaaa tttaaccatg aagccgagga cctgttttat      240

cagagcagcc tggcaagctg gaattataac accaatatta ccgaagagaa cgtgcagaat      300

atgaacaatg ccggtgataa atggtcagcc tttctgaaag aacagagtac cctggcacag      360

atgtatccgc tgcaagaaat tcagaatctg accgttaaac tgcaactgca ggcactgcag      420

cagaatggta gcagcgttct gagcgaagat aaaagcaaac gcctgaacac cattctgaat      480

accatgagta ccatttatag caccggtaaa gtttgcaatc cggataatcc gcaagaatgt      540

ctgctgctgg aaccgggtct gaatgaaatt atggcaaata gcctggatta taacgaacgt      600

ctgtgggcat gggaaagctg gcgtagcgaa gttggtaaac agctgcgtcc gctgtatgaa      660

gaatatgttg ttctgaaaaa tgagatggca cgtgcgaacc actatgaaga ttatggtgat      720

tattggcgtg gcgattatga agttaatggt gtggatggtt atgattatag ccgtggtcag      780

ctgattgaag atgttgaaca taccttcgaa gaaatcaaac cgctgtacga acatctgcat      840

gcctatgtgc gtgcaaaact gatgaatgca tatccgagct atattagccc gattggttgt      900

ctgcctgcac atctgctggg tgatatgtgg ggtcgttttt ggaccaatct gtatagcctg      960

accgttccgt ttggtcagaa accgaatatt gatgttaccg atgcaatggt tgatcaggca     1020

tgggatgcac agcgtatttt caaagaagcc gaaaaattct ttgttagcgt tggtctgccg     1080

aatatgaccc aaggcttttg ggaaaattca atgctgaccg atccgggtaa tgttcagaaa     1140

gcagtttgtc atccgaccgc atgggattta ggtaaaggtg attttcgtat tctgatgtgc     1200

accaaagtga cgatggatga ttttctgacc gcacatcatg aaatgggcca tattcagtat     1260

gatatggcat atgcagcaca gccgtttctg ctgcgtaatg gtgcaaatga aggttttcat     1320

gaagcggttg gcgaaattat gagcctgagc gcagcaaccc cgaaacatct gaaaagcatt     1380

ggtctgctga gtccggattt tcaagaagat aacgaaaccg aaatcaactt cctgctgaaa     1440

caggcactga ccattgttgg caccctgccg tttacctata tgctggaaaa atggcgttgg     1500

atggtgttta aaggtgaaat tccgaaagac cagtggatga aaaaatggtg ggaaatgaaa     1560

cgtgaaattg ttggtgttgt tgaaccggtg ccgcatgatg aaacctattg tgatccggca     1620

agtctgtttc atgtgagcaa cgattatagc ttcatccgtt attatacccg tacgctgtat     1680

cagtttcagt ttcaagaggc actgtgtcag gcagcaaaac atgaaggtcc gctgcataaa     1740

tgtgatatca gcaatagcac cgaagcaggc cagaaactgt ttaatatgct gcgtctgggt     1800

aaaagcgaac cgtggacact ggcactggaa aatgttgttg gtgccaaaaa tatgaatgtt     1860

cgtccgctgc tgaactattt tgaaccgctg tttacatggc tgaaggacca gaataaaaac     1920

agctttgttg gttggagcac cgattggagc ccgtatgccg gcggaagcca tcatcaccac     1980

catcatcacc attga                                                      1995


<210>  22
<211>  664
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-ACE2 (9 G/S linker)

<400>  22

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Ala Ala Gln Ser Thr Ile Glu Glu Gln Ala 
    50                  55                  60                  


Lys Thr Phe Leu Asp Lys Phe Asn His Glu Ala Glu Asp Leu Phe Tyr 
65                  70                  75                  80  


Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu 
                85                  90                  95      


Asn Val Gln Asn Met Asn Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu 
            100                 105                 110         


Lys Glu Gln Ser Thr Leu Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln 
        115                 120                 125             


Asn Leu Thr Val Lys Leu Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser 
    130                 135                 140                 


Ser Val Leu Ser Glu Asp Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn 
145                 150                 155                 160 


Thr Met Ser Thr Ile Tyr Ser Thr Gly Lys Val Cys Asn Pro Asp Asn 
                165                 170                 175     


Pro Gln Glu Cys Leu Leu Leu Glu Pro Gly Leu Asn Glu Ile Met Ala 
            180                 185                 190         


Asn Ser Leu Asp Tyr Asn Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg 
        195                 200                 205             


Ser Glu Val Gly Lys Gln Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val 
    210                 215                 220                 


Leu Lys Asn Glu Met Ala Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp 
225                 230                 235                 240 


Tyr Trp Arg Gly Asp Tyr Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr 
                245                 250                 255     


Ser Arg Gly Gln Leu Ile Glu Asp Val Glu His Thr Phe Glu Glu Ile 
            260                 265                 270         


Lys Pro Leu Tyr Glu His Leu His Ala Tyr Val Arg Ala Lys Leu Met 
        275                 280                 285             


Asn Ala Tyr Pro Ser Tyr Ile Ser Pro Ile Gly Cys Leu Pro Ala His 
    290                 295                 300                 


Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu 
305                 310                 315                 320 


Thr Val Pro Phe Gly Gln Lys Pro Asn Ile Asp Val Thr Asp Ala Met 
                325                 330                 335     


Val Asp Gln Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys 
            340                 345                 350         


Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp Glu 
        355                 360                 365             


Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys His 
    370                 375                 380                 


Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met Cys 
385                 390                 395                 400 


Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met Gly 
                405                 410                 415     


His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu Leu Arg 
            420                 425                 430         


Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile Met Ser 
        435                 440                 445             


Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu Leu Ser 
    450                 455                 460                 


Pro Asp Phe Gln Glu Asp Asn Glu Thr Glu Ile Asn Phe Leu Leu Lys 
465                 470                 475                 480 


Gln Ala Leu Thr Ile Val Gly Thr Leu Pro Phe Thr Tyr Met Leu Glu 
                485                 490                 495     


Lys Trp Arg Trp Met Val Phe Lys Gly Glu Ile Pro Lys Asp Gln Trp 
            500                 505                 510         


Met Lys Lys Trp Trp Glu Met Lys Arg Glu Ile Val Gly Val Val Glu 
        515                 520                 525             


Pro Val Pro His Asp Glu Thr Tyr Cys Asp Pro Ala Ser Leu Phe His 
    530                 535                 540                 


Val Ser Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr 
545                 550                 555                 560 


Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln Ala Ala Lys His Glu Gly 
                565                 570                 575     


Pro Leu His Lys Cys Asp Ile Ser Asn Ser Thr Glu Ala Gly Gln Lys 
            580                 585                 590         


Leu Phe Asn Met Leu Arg Leu Gly Lys Ser Glu Pro Trp Thr Leu Ala 
        595                 600                 605             


Leu Glu Asn Val Val Gly Ala Lys Asn Met Asn Val Arg Pro Leu Leu 
    610                 615                 620                 


Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu Lys Asp Gln Asn Lys Asn 
625                 630                 635                 640 


Ser Phe Val Gly Trp Ser Thr Asp Trp Ser Pro Tyr Ala Gly Gly Ser 
                645                 650                 655     


His His His His His His His His 
            660                 


<210>  23
<211>  1995
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-ACE2 (9 G/S linker)

<400>  23
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg gagtgagcgg ctggcggctg      120

ttcaagaaga ttagcggtgg cggaggatct ggaggtggat ccgcagcaca gagcaccatt      180

gaagaacagg caaaaacctt tctggacaaa tttaaccatg aagccgagga cctgttttat      240

cagagcagcc tggcaagctg gaattataac accaatatta ccgaagagaa cgtgcagaat      300

atgaacaatg ccggtgataa atggtcagcc tttctgaaag aacagagtac cctggcacag      360

atgtatccgc tgcaagaaat tcagaatctg accgttaaac tgcaactgca ggcactgcag      420

cagaatggta gcagcgttct gagcgaagat aaaagcaaac gcctgaacac cattctgaat      480

accatgagta ccatttatag caccggtaaa gtttgcaatc cggataatcc gcaagaatgt      540

ctgctgctgg aaccgggtct gaatgaaatt atggcaaata gcctggatta taacgaacgt      600

ctgtgggcat gggaaagctg gcgtagcgaa gttggtaaac agctgcgtcc gctgtatgaa      660

gaatatgttg ttctgaaaaa tgagatggca cgtgcgaacc actatgaaga ttatggtgat      720

tattggcgtg gcgattatga agttaatggt gtggatggtt atgattatag ccgtggtcag      780

ctgattgaag atgttgaaca taccttcgaa gaaatcaaac cgctgtacga acatctgcat      840

gcctatgtgc gtgcaaaact gatgaatgca tatccgagct atattagccc gattggttgt      900

ctgcctgcac atctgctggg tgatatgtgg ggtcgttttt ggaccaatct gtatagcctg      960

accgttccgt ttggtcagaa accgaatatt gatgttaccg atgcaatggt tgatcaggca     1020

tgggatgcac agcgtatttt caaagaagcc gaaaaattct ttgttagcgt tggtctgccg     1080

aatatgaccc aaggcttttg ggaaaattca atgctgaccg atccgggtaa tgttcagaaa     1140

gcagtttgtc atccgaccgc atgggattta ggtaaaggtg attttcgtat tctgatgtgc     1200

accaaagtga cgatggatga ttttctgacc gcacatcatg aaatgggcca tattcagtat     1260

gatatggcat atgcagcaca gccgtttctg ctgcgtaatg gtgcaaatga aggttttcat     1320

gaagcggttg gcgaaattat gagcctgagc gcagcaaccc cgaaacatct gaaaagcatt     1380

ggtctgctga gtccggattt tcaagaagat aacgaaaccg aaatcaactt cctgctgaaa     1440

caggcactga ccattgttgg caccctgccg tttacctata tgctggaaaa atggcgttgg     1500

atggtgttta aaggtgaaat tccgaaagac cagtggatga aaaaatggtg ggaaatgaaa     1560

cgtgaaattg ttggtgttgt tgaaccggtg ccgcatgatg aaacctattg tgatccggca     1620

agtctgtttc atgtgagcaa cgattatagc ttcatccgtt attatacccg tacgctgtat     1680

cagtttcagt ttcaagaggc actgtgtcag gcagcaaaac atgaaggtcc gctgcataaa     1740

tgtgatatca gcaatagcac cgaagcaggc cagaaactgt ttaatatgct gcgtctgggt     1800

aaaagcgaac cgtggacact ggcactggaa aatgttgttg gtgccaaaaa tatgaatgtt     1860

cgtccgctgc tgaactattt tgaaccgctg tttacatggc tgaaggacca gaataaaaac     1920

agctttgttg gttggagcac cgattggagc ccgtatgccg gcggaagcca tcatcaccac     1980

catcatcacc attga                                                      1995


<210>  24
<211>  664
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta10-ACE2 (9 G/S linker)

<400>  24

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Val Ser Gly Trp Arg Leu Phe Lys Lys Ile Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Ala Ala Gln Ser Thr Ile Glu Glu Gln Ala 
    50                  55                  60                  


Lys Thr Phe Leu Asp Lys Phe Asn His Glu Ala Glu Asp Leu Phe Tyr 
65                  70                  75                  80  


Gln Ser Ser Leu Ala Ser Trp Asn Tyr Asn Thr Asn Ile Thr Glu Glu 
                85                  90                  95      


Asn Val Gln Asn Met Asn Asn Ala Gly Asp Lys Trp Ser Ala Phe Leu 
            100                 105                 110         


Lys Glu Gln Ser Thr Leu Ala Gln Met Tyr Pro Leu Gln Glu Ile Gln 
        115                 120                 125             


Asn Leu Thr Val Lys Leu Gln Leu Gln Ala Leu Gln Gln Asn Gly Ser 
    130                 135                 140                 


Ser Val Leu Ser Glu Asp Lys Ser Lys Arg Leu Asn Thr Ile Leu Asn 
145                 150                 155                 160 


Thr Met Ser Thr Ile Tyr Ser Thr Gly Lys Val Cys Asn Pro Asp Asn 
                165                 170                 175     


Pro Gln Glu Cys Leu Leu Leu Glu Pro Gly Leu Asn Glu Ile Met Ala 
            180                 185                 190         


Asn Ser Leu Asp Tyr Asn Glu Arg Leu Trp Ala Trp Glu Ser Trp Arg 
        195                 200                 205             


Ser Glu Val Gly Lys Gln Leu Arg Pro Leu Tyr Glu Glu Tyr Val Val 
    210                 215                 220                 


Leu Lys Asn Glu Met Ala Arg Ala Asn His Tyr Glu Asp Tyr Gly Asp 
225                 230                 235                 240 


Tyr Trp Arg Gly Asp Tyr Glu Val Asn Gly Val Asp Gly Tyr Asp Tyr 
                245                 250                 255     


Ser Arg Gly Gln Leu Ile Glu Asp Val Glu His Thr Phe Glu Glu Ile 
            260                 265                 270         


Lys Pro Leu Tyr Glu His Leu His Ala Tyr Val Arg Ala Lys Leu Met 
        275                 280                 285             


Asn Ala Tyr Pro Ser Tyr Ile Ser Pro Ile Gly Cys Leu Pro Ala His 
    290                 295                 300                 


Leu Leu Gly Asp Met Trp Gly Arg Phe Trp Thr Asn Leu Tyr Ser Leu 
305                 310                 315                 320 


Thr Val Pro Phe Gly Gln Lys Pro Asn Ile Asp Val Thr Asp Ala Met 
                325                 330                 335     


Val Asp Gln Ala Trp Asp Ala Gln Arg Ile Phe Lys Glu Ala Glu Lys 
            340                 345                 350         


Phe Phe Val Ser Val Gly Leu Pro Asn Met Thr Gln Gly Phe Trp Glu 
        355                 360                 365             


Asn Ser Met Leu Thr Asp Pro Gly Asn Val Gln Lys Ala Val Cys His 
    370                 375                 380                 


Pro Thr Ala Trp Asp Leu Gly Lys Gly Asp Phe Arg Ile Leu Met Cys 
385                 390                 395                 400 


Thr Lys Val Thr Met Asp Asp Phe Leu Thr Ala His His Glu Met Gly 
                405                 410                 415     


His Ile Gln Tyr Asp Met Ala Tyr Ala Ala Gln Pro Phe Leu Leu Arg 
            420                 425                 430         


Asn Gly Ala Asn Glu Gly Phe His Glu Ala Val Gly Glu Ile Met Ser 
        435                 440                 445             


Leu Ser Ala Ala Thr Pro Lys His Leu Lys Ser Ile Gly Leu Leu Ser 
    450                 455                 460                 


Pro Asp Phe Gln Glu Asp Asn Glu Thr Glu Ile Asn Phe Leu Leu Lys 
465                 470                 475                 480 


Gln Ala Leu Thr Ile Val Gly Thr Leu Pro Phe Thr Tyr Met Leu Glu 
                485                 490                 495     


Lys Trp Arg Trp Met Val Phe Lys Gly Glu Ile Pro Lys Asp Gln Trp 
            500                 505                 510         


Met Lys Lys Trp Trp Glu Met Lys Arg Glu Ile Val Gly Val Val Glu 
        515                 520                 525             


Pro Val Pro His Asp Glu Thr Tyr Cys Asp Pro Ala Ser Leu Phe His 
    530                 535                 540                 


Val Ser Asn Asp Tyr Ser Phe Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr 
545                 550                 555                 560 


Gln Phe Gln Phe Gln Glu Ala Leu Cys Gln Ala Ala Lys His Glu Gly 
                565                 570                 575     


Pro Leu His Lys Cys Asp Ile Ser Asn Ser Thr Glu Ala Gly Gln Lys 
            580                 585                 590         


Leu Phe Asn Met Leu Arg Leu Gly Lys Ser Glu Pro Trp Thr Leu Ala 
        595                 600                 605             


Leu Glu Asn Val Val Gly Ala Lys Asn Met Asn Val Arg Pro Leu Leu 
    610                 615                 620                 


Asn Tyr Phe Glu Pro Leu Phe Thr Trp Leu Lys Asp Gln Asn Lys Asn 
625                 630                 635                 640 


Ser Phe Val Gly Trp Ser Thr Asp Trp Ser Pro Tyr Ala Gly Gly Ser 
                645                 650                 655     


His His His His His His His His 
            660                 


<210>  25
<211>  2730
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-ACE2-Fc (18 G/S linker)

<400>  25
atgcggcttc cgggtgcgat gccagctctg gccctcaaag gcgagctgct gttgctgtct       60

ctcctgttac ttctggaacc acagatctct cagggcggtg ggggctccat gctgttccga      120

gtaaccatca acagtggtgg cggaggatct ggaggtggat ccggtggcgg aggatctgga      180

ggtggatccg cagcacagag caccattgaa gaacaggcaa aaacctttct ggacaaattt      240

aaccatgaag ccgaggacct gttttatcag agcagcctgg caagctggaa ttataacacc      300

aatattaccg aagagaacgt gcagaatatg aacaatgccg gtgataaatg gtcagccttt      360

ctgaaagaac agagtaccct ggcacagatg tatccgctgc aagaaattca gaatctgacc      420

gttaaactgc aactgcaggc actgcagcag aatggtagca gcgttctgag cgaagataaa      480

agcaaacgcc tgaacaccat tctgaatacc atgagtacca tttatagcac cggtaaagtt      540

tgcaatccgg ataatccgca agaatgtctg ctgctggaac cgggtctgaa tgaaattatg      600

gcaaatagcc tggattataa cgaacgtctg tgggcatggg aaagctggcg tagcgaagtt      660

ggtaaacagc tgcgtccgct gtatgaagaa tatgttgttc tgaaaaatga gatggcacgt      720

gcgaaccact atgaagatta tggtgattat tggcgtggcg attatgaagt taatggtgtg      780

gatggttatg attatagccg tggtcagctg attgaagatg ttgaacatac cttcgaagaa      840

atcaaaccgc tgtacgaaca tctgcatgcc tatgtgcgtg caaaactgat gaatgcatat      900

ccgagctata ttagcccgat tggttgtctg cctgcacatc tgctgggtga tatgtggggt      960

cgtttttgga ccaatctgta tagcctgacc gttccgtttg gtcagaaacc gaatattgat     1020

gttaccgatg caatggttga tcaggcatgg gatgcacagc gtattttcaa agaagccgaa     1080

aaattctttg ttagcgttgg tctgccgaat atgacccaag gcttttggga aaattcaatg     1140

ctgaccgatc cgggtaatgt tcagaaagca gtttgtcatc cgaccgcatg ggatttaggt     1200

aaaggtgatt ttcgtattct gatgtgcacc aaagtgacga tggatgattt tctgaccgca     1260

catcatgaaa tgggccatat tcagtatgat atggcatatg cagcacagcc gtttctgctg     1320

cgtaatggtg caaatgaagg ttttcatgaa gcggttggcg aaattatgag cctgagcgca     1380

gcaaccccga aacatctgaa aagcattggt ctgctgagtc cggattttca agaagataac     1440

gaaaccgaaa tcaacttcct gctgaaacag gcactgacca ttgttggcac cctgccgttt     1500

acctatatgc tggaaaaatg gcgttggatg gtgtttaaag gtgaaattcc gaaagaccag     1560

tggatgaaaa aatggtggga aatgaaacgt gaaattgttg gtgttgttga accggtgccg     1620

catgatgaaa cctattgtga tccggcaagt ctgtttcatg tgagcaacga ttatagcttc     1680

atccgttatt atacccgtac gctgtatcag tttcagtttc aagaggcact gtgtcaggca     1740

gcaaaacatg aaggtccgct gcataaatgt gatatcagca atagcaccga agcaggccag     1800

aaactgttta atatgctgcg tctgggtaaa agcgaaccgt ggacactggc actggaaaat     1860

gttgttggtg ccaaaaatat gaatgttcgt ccgctgctga actattttga accgctgttt     1920

acatggctga aggaccagaa taaaaacagc tttgttggtt ggagcaccga ttggagcccg     1980

tatgccggcg gaagcggagg cggaggttct ggagggggag gtagcacatg cccaccgtgc     2040

ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa acccaaggac     2100

accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt gagccacgaa     2160

gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa tgccaagaca     2220

aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct caccgtcctg     2280

caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa agccctccca     2340

gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc acaggtgtac     2400

accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac ctgcctggtc     2460

aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca gccggagaac     2520

aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct ctacagcaag     2580

ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc cgtgatgcat     2640

gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg taaaggcgga     2700

agccatcatc accaccatca tcaccattga                                      2730


<210>  26
<211>  909
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: Beta9-ACE2-Fc (18 G/S linker)

<400>  26

Met Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu 
1               5                   10                  15      


Leu Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30          


Gly Gly Gly Ser Met Leu Phe Arg Val Thr Ile Asn Ser Gly Gly Gly 
        35                  40                  45              


Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Ser Ala 
    50                  55                  60                  


Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
65                  70                  75                  80  


Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
                85                  90                  95      


Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
            100                 105                 110         


Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
        115                 120                 125             


Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
    130                 135                 140                 


Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
145                 150                 155                 160 


Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
                165                 170                 175     


Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
            180                 185                 190         


Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
        195                 200                 205             


Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
    210                 215                 220                 


Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
225                 230                 235                 240 


Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
                245                 250                 255     


Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
            260                 265                 270         


Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
        275                 280                 285             


His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
    290                 295                 300                 


Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
305                 310                 315                 320 


Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
                325                 330                 335     


Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
            340                 345                 350         


Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
        355                 360                 365             


Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
    370                 375                 380                 


Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
385                 390                 395                 400 


Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
                405                 410                 415     


Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
            420                 425                 430         


Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
        435                 440                 445             


His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
    450                 455                 460                 


His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
465                 470                 475                 480 


Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
                485                 490                 495     


Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
            500                 505                 510         


Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
        515                 520                 525             


Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
    530                 535                 540                 


Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
545                 550                 555                 560 


Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
                565                 570                 575     


Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
            580                 585                 590         


Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
        595                 600                 605             


Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
    610                 615                 620                 


Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
625                 630                 635                 640 


Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
                645                 650                 655     


Asp Trp Ser Pro Tyr Ala Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            660                 665                 670         


Gly Gly Ser Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly 
        675                 680                 685             


Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile 
    690                 695                 700                 


Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu 
705                 710                 715                 720 


Asp Pro Glu Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His 
                725                 730                 735     


Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg 
            740                 745                 750         


Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys 
        755                 760                 765             


Glu Tyr Lys Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu 
    770                 775                 780                 


Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr 
785                 790                 795                 800 


Thr Leu Pro Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu 
                805                 810                 815     


Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp 
            820                 825                 830         


Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val 
        835                 840                 845             


Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp 
    850                 855                 860                 


Lys Ser Arg Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His 
865                 870                 875                 880 


Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro 
                885                 890                 895     


Gly Lys Gly Gly Ser His His His His His His His His 
            900                 905                 


<210>  27
<211>  805
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: ACE2

<400>  27

Met Ser Ser Ser Ser Trp Leu Leu Leu Ser Leu Val Ala Val Thr Ala 
1               5                   10                  15      


Ala Gln Ser Thr Ile Glu Glu Gln Ala Lys Thr Phe Leu Asp Lys Phe 
            20                  25                  30          


Asn His Glu Ala Glu Asp Leu Phe Tyr Gln Ser Ser Leu Ala Ser Trp 
        35                  40                  45              


Asn Tyr Asn Thr Asn Ile Thr Glu Glu Asn Val Gln Asn Met Asn Asn 
    50                  55                  60                  


Ala Gly Asp Lys Trp Ser Ala Phe Leu Lys Glu Gln Ser Thr Leu Ala 
65                  70                  75                  80  


Gln Met Tyr Pro Leu Gln Glu Ile Gln Asn Leu Thr Val Lys Leu Gln 
                85                  90                  95      


Leu Gln Ala Leu Gln Gln Asn Gly Ser Ser Val Leu Ser Glu Asp Lys 
            100                 105                 110         


Ser Lys Arg Leu Asn Thr Ile Leu Asn Thr Met Ser Thr Ile Tyr Ser 
        115                 120                 125             


Thr Gly Lys Val Cys Asn Pro Asp Asn Pro Gln Glu Cys Leu Leu Leu 
    130                 135                 140                 


Glu Pro Gly Leu Asn Glu Ile Met Ala Asn Ser Leu Asp Tyr Asn Glu 
145                 150                 155                 160 


Arg Leu Trp Ala Trp Glu Ser Trp Arg Ser Glu Val Gly Lys Gln Leu 
                165                 170                 175     


Arg Pro Leu Tyr Glu Glu Tyr Val Val Leu Lys Asn Glu Met Ala Arg 
            180                 185                 190         


Ala Asn His Tyr Glu Asp Tyr Gly Asp Tyr Trp Arg Gly Asp Tyr Glu 
        195                 200                 205             


Val Asn Gly Val Asp Gly Tyr Asp Tyr Ser Arg Gly Gln Leu Ile Glu 
    210                 215                 220                 


Asp Val Glu His Thr Phe Glu Glu Ile Lys Pro Leu Tyr Glu His Leu 
225                 230                 235                 240 


His Ala Tyr Val Arg Ala Lys Leu Met Asn Ala Tyr Pro Ser Tyr Ile 
                245                 250                 255     


Ser Pro Ile Gly Cys Leu Pro Ala His Leu Leu Gly Asp Met Trp Gly 
            260                 265                 270         


Arg Phe Trp Thr Asn Leu Tyr Ser Leu Thr Val Pro Phe Gly Gln Lys 
        275                 280                 285             


Pro Asn Ile Asp Val Thr Asp Ala Met Val Asp Gln Ala Trp Asp Ala 
    290                 295                 300                 


Gln Arg Ile Phe Lys Glu Ala Glu Lys Phe Phe Val Ser Val Gly Leu 
305                 310                 315                 320 


Pro Asn Met Thr Gln Gly Phe Trp Glu Asn Ser Met Leu Thr Asp Pro 
                325                 330                 335     


Gly Asn Val Gln Lys Ala Val Cys His Pro Thr Ala Trp Asp Leu Gly 
            340                 345                 350         


Lys Gly Asp Phe Arg Ile Leu Met Cys Thr Lys Val Thr Met Asp Asp 
        355                 360                 365             


Phe Leu Thr Ala His His Glu Met Gly His Ile Gln Tyr Asp Met Ala 
    370                 375                 380                 


Tyr Ala Ala Gln Pro Phe Leu Leu Arg Asn Gly Ala Asn Glu Gly Phe 
385                 390                 395                 400 


His Glu Ala Val Gly Glu Ile Met Ser Leu Ser Ala Ala Thr Pro Lys 
                405                 410                 415     


His Leu Lys Ser Ile Gly Leu Leu Ser Pro Asp Phe Gln Glu Asp Asn 
            420                 425                 430         


Glu Thr Glu Ile Asn Phe Leu Leu Lys Gln Ala Leu Thr Ile Val Gly 
        435                 440                 445             


Thr Leu Pro Phe Thr Tyr Met Leu Glu Lys Trp Arg Trp Met Val Phe 
    450                 455                 460                 


Lys Gly Glu Ile Pro Lys Asp Gln Trp Met Lys Lys Trp Trp Glu Met 
465                 470                 475                 480 


Lys Arg Glu Ile Val Gly Val Val Glu Pro Val Pro His Asp Glu Thr 
                485                 490                 495     


Tyr Cys Asp Pro Ala Ser Leu Phe His Val Ser Asn Asp Tyr Ser Phe 
            500                 505                 510         


Ile Arg Tyr Tyr Thr Arg Thr Leu Tyr Gln Phe Gln Phe Gln Glu Ala 
        515                 520                 525             


Leu Cys Gln Ala Ala Lys His Glu Gly Pro Leu His Lys Cys Asp Ile 
    530                 535                 540                 


Ser Asn Ser Thr Glu Ala Gly Gln Lys Leu Phe Asn Met Leu Arg Leu 
545                 550                 555                 560 


Gly Lys Ser Glu Pro Trp Thr Leu Ala Leu Glu Asn Val Val Gly Ala 
                565                 570                 575     


Lys Asn Met Asn Val Arg Pro Leu Leu Asn Tyr Phe Glu Pro Leu Phe 
            580                 585                 590         


Thr Trp Leu Lys Asp Gln Asn Lys Asn Ser Phe Val Gly Trp Ser Thr 
        595                 600                 605             


Asp Trp Ser Pro Tyr Ala Asp Gln Ser Ile Lys Val Arg Ile Ser Leu 
    610                 615                 620                 


Lys Ser Ala Leu Gly Asp Arg Ala Tyr Glu Trp Asn Asp Asn Glu Met 
625                 630                 635                 640 


Tyr Leu Phe Arg Ser Ser Val Ala Tyr Ala Met Arg Gln Tyr Phe Leu 
                645                 650                 655     


Lys Val Lys Asn Gln Met Ile Leu Phe Gly Glu Glu Asp Val Arg Val 
            660                 665                 670         


Ala Asn Leu Lys Pro Arg Ile Ser Phe Asn Phe Phe Val Thr Ala Pro 
        675                 680                 685             


Lys Asn Val Ser Asp Ile Ile Pro Arg Thr Glu Val Glu Lys Ala Ile 
    690                 695                 700                 


Arg Met Ser Arg Ser Arg Ile Asn Asp Ala Phe Arg Leu Asn Asp Asn 
705                 710                 715                 720 


Ser Leu Glu Phe Leu Gly Ile Gln Pro Thr Leu Gly Pro Pro Asn Gln 
                725                 730                 735     


Pro Pro Val Ser Ile Trp Leu Ile Val Phe Gly Val Val Met Gly Val 
            740                 745                 750         


Ile Val Val Gly Ile Val Ile Leu Ile Phe Thr Gly Ile Arg Asp Arg 
        755                 760                 765             


Lys Lys Lys Asn Lys Ala Arg Ser Gly Glu Asn Pro Tyr Ala Ser Ile 
    770                 775                 780                 


Asp Ile Ser Lys Gly Glu Asn Asn Pro Gly Phe Gln Asn Thr Asp Asp 
785                 790                 795                 800 


Val Gln Thr Ser Phe 
                805 


<210>  28
<211>  223
<212>  PRT
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human IgG1 Fc

<400>  28

Thr Cys Pro Pro Cys Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val 
1               5                   10                  15      


Phe Leu Phe Pro Pro Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr 
            20                  25                  30          


Pro Glu Val Thr Cys Val Val Val Asp Val Ser His Glu Asp Pro Glu 
        35                  40                  45              


Val Lys Phe Asn Trp Tyr Val Asp Gly Val Glu Val His Asn Ala Lys 
    50                  55                  60                  


Thr Lys Pro Arg Glu Glu Gln Tyr Asn Ser Thr Tyr Arg Val Val Ser 
65                  70                  75                  80  


Val Leu Thr Val Leu His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys 
                85                  90                  95      


Cys Lys Val Ser Asn Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile 
            100                 105                 110         


Ser Lys Ala Lys Gly Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro 
        115                 120                 125             


Pro Ser Arg Asp Glu Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu 
    130                 135                 140                 


Val Lys Gly Phe Tyr Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn 
145                 150                 155                 160 


Gly Gln Pro Glu Asn Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser 
                165                 170                 175     


Asp Gly Ser Phe Phe Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg 
            180                 185                 190         


Trp Gln Gln Gly Asn Val Phe Ser Cys Ser Val Met His Glu Ala Leu 
        195                 200                 205             


His Asn His Tyr Thr Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
    210                 215                 220             


<210>  29
<211>  31
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: PDGFRB signal peptide

<400>  29

Arg Leu Pro Gly Ala Met Pro Ala Leu Ala Leu Lys Gly Glu Leu Leu 
1               5                   10                  15      


Leu Leu Ser Leu Leu Leu Leu Leu Glu Pro Gln Ile Ser Gln Gly 
            20                  25                  30      


